LOCUS       XM_030064345           12710 bp    mRNA    linear   VRT 08-JUL-2025
DEFINITION  PREDICTED: Myripristis murdjan argonaute RISC catalytic component 2
            (ago2), mRNA.
ACCESSION   XM_030064345
VERSION     XM_030064345.1
DBLINK      BioProject: PRJNA556033
KEYWORDS    RefSeq.
SOURCE      Myripristis murdjan (pinecone soldierfish)
  ORGANISM  Myripristis murdjan
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Holocentriformes; Holocentridae; Myripristis.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_043990.1) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Updated annotation
            Annotation Name             :: GCF_902150065.1-RS_2025_07
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.4
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 07/03/2025
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12710
                     /organism="Myripristis murdjan"
                     /mol_type="mRNA"
                     /db_xref="taxon:586833"
                     /chromosome="11"
     gene            1..12710
                     /gene="ago2"
                     /note="argonaute RISC catalytic component 2; Derived by
                     automated computational analysis using gene prediction
                     method: Gnomon. Supporting evidence includes similarity
                     to: 20 Proteins, and 100% coverage of the annotated
                     genomic feature by RNAseq alignments, including 5 samples
                     with support for all annotated introns"
                     /db_xref="GeneID:115368287"
     CDS             231..2843
                     /gene="ago2"
                     /codon_start=1
                     /product="protein argonaute-2"
                     /protein_id="XP_029920205.1"
                     /db_xref="GeneID:115368287"
                     /translation="

MYSSAGAAEMLEGPLSSGSIGSDPPSPPVPEYVFKPPSRPDFGTMGRTIKLQANFFEMEIPKLEVYHYDIDIKPEKCPRRVNREIVEHMVQHFKTQIFGDRKPVYDGRKNLYTAMPLPIGRDKVELEVTIPGEGKDRSFKVAIKWVSCVSLQALHEALSGRLPNIPFETIQALDVVMRHLPSMRYTPVGRSFFTPSEGCSNPLGGGREVWFGFHQSVRPSLWKMMLNIDVSATAFYKAQPVIEFMCEVLDFKSIEEQQKPLTDSQRVKFTKEIKGLKVEITHCGQMKRKYRVCNVTRRPASHQTFPLQQENGQTIECTVAQYFKDKYKLILRYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIRATARSAPDRQDEISKLMRSANFNTDPYVREFGVMVRDEMTEVNGRVLQAPSILYGGRNKAIATPIQGVWDMRNKQFHTGIEIKVWAIACFAPQRQCTEILLKAFTDQLRKISRDAGMPIQGQPCFCKYAQGADSVEPMFKHLKYTYQGLQLVVVILPGKTPVYAEVKRVGDTVLGMATQCVQVKNVQKTTPQTLSNLCLKINVKLGGVNNILLPQGRPLVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDAHPSRYCATVRVQQHRQDIIQDLATMVRELLIQFYKSTRFKPTRIIYYRDGISEGQFNQVLQHELLAIREACIKLEKDYQPGITFVVVQKRHHTRLFCMDRNERVGKSGNIPAGTTVDTKITHPSEFDFYLCSHAGIQGTSRPSHYHVLWDDNHFTSDELQVLTYQLCHTYVRCTRSVSIPAPAYYAHLVAFRARYHLVDKEHDSAEGSHTSGQSNGRDQQALAKAVQIHQDTLRTMYFA"

     misc_feature    537..761
                     /gene="ago2"
                     /note="N-terminal domain of argonaute; Region: ArgoN;
                     pfam16486"
                     /db_xref="CDD:465134"
     misc_feature    789..941
                     /gene="ago2"
                     /note="Argonaute linker 1 domain; Region: ArgoL1;
                     pfam08699"
                     /db_xref="CDD:462567"
     misc_feature    942..1304
                     /gene="ago2"
                     /note="PAZ domain, argonaute_like subfamily. Argonaute is
                     part of the RNA-induced silencing complex (RISC), and is
                     an endonuclease that plays a key role in the RNA
                     interference pathway. The PAZ domain has been named after
                     the proteins Piwi,Argonaut, and Zwille; Region:
                     PAZ_argonaute_like; cd02846"
                     /db_xref="CDD:239212"
     misc_feature    order(1098..1100,1143..1145,1185..1187,1197..1199,
                     1251..1253,1272..1274,1278..1280)
                     /gene="ago2"
                     /note="nucleic acid-binding interface [nucleotide
                     binding]; other site"
                     /db_xref="CDD:239212"
     misc_feature    1437..2714
                     /gene="ago2"
                     /note="PIWI domain, Argonaute-like subfamily. Argonaute is
                     the central component of the RNA-induced silencing complex
                     (RISC) and related complexes. The PIWI domain is the
                     C-terminal portion of Argonaute and consists of two
                     subdomains, one of which provides the...; Region:
                     Piwi_ago-like; cd04657"
                     /db_xref="CDD:240015"
     misc_feature    order(1848..1850,1860..1862,1896..1907,1914..1916,
                     1938..1940,1947..1949,1959..1961,1971..1973)
                     /gene="ago2"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240015"
     misc_feature    order(2052..2054,2058..2060,2268..2270,2682..2684)
                     /gene="ago2"
                     /note="active site"
                     /db_xref="CDD:240015"
ORIGIN      

atctcggctcccgttttgtgtgttggggggatggggccgtgccgaggatgagaaagctctcgtggtgcaaatatttaccacttgactaactaaaatatttcttcccgaactggacttccctcgctctttttgtacagagatactttcgggccacgtatgtgtttgggaggcatacgggaacagtaaagtggagtacccgtacgtccacggcacacgcttaaatcgacaaaatgtattcctctgctggagctgctgagatgcttgagggacccctttcatctgggtcaataggctctgaccccccatctcccccagtgccagagtatgtgttcaagccgccatcacggccagactttggcaccatgggcaggacaatcaagctgcaggctaacttctttgagatggaaatccccaaactagaggtgtaccattacgacattgacatcaagcctgagaaatgccccaggagggtcaatcgggaaattgtggagcacatggtccagcactttaaaacacaaatttttggggatcgaaaacctgtatatgacgggaggaagaatctctacacagccatgcccttgcctataggcagagacaaagtggagcttgaagtgaccattcccggtgaaggcaaggaccgcagcttcaaagtggccatcaagtgggtgtcctgtgttagtctgcaggctctgcacgaggcgctatcgggacggctacccaacatcccctttgagaccattcaagccctggacgtggtcatgagacatttgccctctatgaggtatactcccgtgggccgctccttcttcactccctcagaaggatgttcaaaccccctcggtgggggcagagaggtgtggtttgggttccaccagtcggtcaggccttccctctggaaaatgatgctcaacattgatgtttcggccactgcattctacaaagcccagcctgtaattgagttcatgtgtgaggtcttggatttcaaaagcattgaagaacaacagaagcccttaacagactctcagagagtaaaatttactaaggaaatcaaaggtttgaaagtggagatcactcactgtgggcagatgaagaggaagtacagagtatgcaatgtgaccaggagaccagccagccaccagacgttccctctgcagcaggagaatggccaaactattgaatgcacagtagcacagtatttcaaagacaagtacaagctgattctgcggtatccacacctcccatgtctccaggtgggacaggagcagaagcacacctacctgcctctagaggtgtgtaacatagtggcaggccagcgctgcatcaagaagctaacagacaatcagacctccactatgatccgtgccacagcccgatcagcacccgaccgtcaggatgagatcagcaaattgatgagaagtgccaactttaacactgacccttatgttcgggagtttggagtgatggtgagggatgagatgacagaagtgaacggccgcgtattacaggcaccttccattctctatggaggcaggaacaaagcaatagctacaccaatccagggagtgtgggacatgaggaacaagcaattccacaccggcattgagatcaaagtgtgggccatcgcttgctttgccccgcagagacagtgcactgaaattctgctcaaagcatttacagatcagctgaggaaaatctctcgggatgcggggatgccgatccagggtcagccttgcttctgtaagtacgcccagggagccgacagcgtggagcccatgttcaaacacctcaaatacacctaccagggcctccagctggtggtggtcatcctgccagggaagacgccagtctatgctgaggtgaagcgtgttggtgacacggtgctgggcatggctacacagtgtgtgcaggtgaagaacgttcagaaaaccacacctcagaccctctccaacctctgtctgaagatcaatgtcaagctgggcggtgtcaacaacatcctcctccctcagggcaggcctctggtgttccagcagcctgtcattttccttggtgcagatgtgacacatccgcctgcaggagatgggaagaagccctctatcgctgctgtggtcggcagtatggatgctcaccccagccgctattgtgctacagtgcgggtccagcagcaccgccaggatatcatccaggacctggccacaatggtgagggagctgctcatccagttttacaagtccacacgcttcaagcccactcgtatcatctactaccgcgatggcatttctgagggccagttcaaccaggttcttcagcacgagctgttggcaatccgcgaggcctgcatcaagctagagaaggactaccagcctggcatcacctttgtggttgtgcagaagagacatcacaccagattgttctgcatggacaggaatgagcgggttggcaagagtggcaacattcctgcaggcaccacagtggacaccaaaatcactcatccgtcagagtttgacttctacctttgcagtcacgctggcatacagggcacaagcaggccatctcactaccatgtgctctgggacgacaaccacttcacttcggatgagctgcaggtcctcacctaccagctgtgccacacctatgtgcgctgcacccggtcagtctccatcccagcaccagcctactatgcccacttggtggctttccgcgcccgctatcacttggtcgacaaagagcacgacagtgctgaaggcagtcacacatcaggccagagcaatgggcgtgaccagcaggccttggccaaggctgttcagatccaccaggacacccttcgcaccatgtactttgcctgagtatgcaggttggcgggggtgagatcccactgctggaacccagtacacctccctgcctcactgtcaggtgtagatacatcacagtggtctcaaacagtgaattccctacccagcaggcctgtcaattttccagtgtttcctcaatcatataagcccacatgtgcaagcccctaacccacccaaacgttctagcgattagactgtaaagtttaaaaaaatcctctgtattggggtttggatggcggagttattactggtatttacatattgtgaattagtgtgtatttgtaaatgtcatggggtcatgtgtttacaaagtatgagttaaaggtagagaggtttgtagactgacagaagaaatctaccatttctacactagtgggcagtatcattctataatgggtgtggtttagacttcagaagaacccatccactttatctttctcctgtttttattctatttgataggggcctggtgcttgaacagaggagggggcatcaaaagatcgggagcggggggcactcacagttcataggcccaatcattttgttcctctgacaaacattatttatcagccaatcaaagcaaagtgcactgaccagaatggatactttaatcaggacgaaagtacttaatatatcgaaggttgcgtctcaaatcctactttaaattctcacgtagggcactatgtgaagaaaagtgccatttgagaccaccattgtgttgacatttttatgtacagctatgaatcaagtggaccgcatttgctgggtttttcagtatttgtccttgattgacactggtgaccaacatgtttttatgtcaggtttgctgacaaacatcagatttgtatatgacccttttaaaccaacacttattttattttagaagagggcctttaattttgatgagattgaaagaacagcaattttctgctttcctgcctcgcttcttacttggaattcattaagttgcatcacttatctttttcccatgctgccgagaaatggttgggactggctcccgacaaggacacctttcaagtgtactctgagccgtgttgatacgagaatacatgatacagacatctacatgggggaacaataatgaaatcttgcgtgtccccatctgtttttatagtctcgctattttaattttaatcgtttttgtaatgaatatgcatgtagatgactcatattttttagacgtttaggtatttgtgataattgtttattttttttcttttagacacattaaccctagttctgttttttttatatatatatatatatatggcagcaaaatagtctctctgcagtgtcattgtcagaactggatgttggtctggcaacttagtatccaggcgtttggtacagtagaatgagacctgcaacatttgcctctgtttcaacagagcctggagtgaagaatgacagagtaaaatttaattgatcaagattccctaagtcaatttcattatagtggttgctccatgaattaggagcacttgtggccacacaaaagcttggattttatgcaaatgattttttgtgagtctttatagtatgcagaattgttgattatagggtatattttgaataagctttcaggggagactgataaaactattatagaagcagttttttaaaaaatgactggcccatatgtactccaaattaacactgtctatttgtatacacagaacttaaaatgcaaatgttctattgccttccaagtctgtcgctgatattgtactaataactctagacatttcctagcttcccatctaagttttaagttttactacttacaggttaagttggcgaatgttcatatagtcttgattggaggggaataatctctattttctaactatgcattgtttttaaccaagagatttagcagtggggtttctctcatgaatttgggtcatttatagaggtagctaatcagggcttttatgtttgtttgttgttgatgtttttttttgttttttttttcctttgcatttttgcatttagtagcgtttcttagtgcttattagtatcttcatgcctttgtaacggtgtgaaggtttaatgtcctcatttgtattgttgtagattttatagcacaaaaattgggtatatcctttgtaacacacaatctttcttagc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