LOCUS       XM_029493919            3778 bp    mRNA    linear   VRT 15-JUL-2025
DEFINITION  PREDICTED: Echeneis naucrates NRDE-2, necessary for RNA
            interference, domain containing (nrde2), mRNA.
ACCESSION   XM_029493919
VERSION     XM_029493919.1
DBLINK      BioProject: PRJNA548465
KEYWORDS    RefSeq.
SOURCE      Echeneis naucrates (live sharksucker)
  ORGANISM  Echeneis naucrates
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Carangaria; Carangiformes; Echeneidae; Echeneis.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_042532.1) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Updated annotation
            Annotation Name             :: GCF_900963305.1-RS_2025_07
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.4
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 07/15/2025
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3778
                     /organism="Echeneis naucrates"
                     /mol_type="mRNA"
                     /db_xref="taxon:173247"
                     /chromosome="22"
     gene            1..3778
                     /gene="nrde2"
                     /note="NRDE-2, necessary for RNA interference, domain
                     containing; Derived by automated computational analysis
                     using gene prediction method: Gnomon. Supporting evidence
                     includes similarity to: 1 Protein, and 100% coverage of
                     the annotated genomic feature by RNAseq alignments,
                     including 1 sample with support for all annotated introns"
                     /db_xref="GeneID:115035880"
     CDS             18..3614
                     /gene="nrde2"
                     /codon_start=1
                     /product="protein NRDE2 homolog"
                     /protein_id="XP_029349779.1"
                     /db_xref="GeneID:115035880"
                     /translation="

MALFPAFAETSSNKVEDSSKDWLNNKSFQSTEALSLHKRFLENKEPSVDKEVSSAEEKREEDVPPKKKKKKSGKRKKKKKHKKKSGGHSDSSGSDSDTIYPSDLKREEEAKKSQAAPLASCFSWLDDLQSPTEHPFCVDRKPDPANWTYKSLYRGDVARYKRKGRSSLGLDPVRQGVSWEESESNKKQKSGEKKKGDNRYFSRLSRQILRSNAAVPTLPTVPECSDVISTSSFLLLGDDEGANKEKETSDRVQTSSVNPLGVYDSSTALWLQGKGQQDKTEEQNQDIEAGQGALLMARRTEEFNRQLREQPADTQLWIKFIRYQDEVSLAEFGSEEMQQDHDSTERRKSSYRAVLEKKLSIAERAVDTNPTCIALQLERLRICQELWEPSVLAREWKKLVFLHPNSAPLWREYLLFTQSYFSNFTVPKVNSAYGKCLSTLSAVRDGSMVSHPALPGIEEDMLDIFIQQCHFLRQCGHSEKAVSLFQAMMDFTFYKPDSVREMSTKQQAEFFEPFWDSGEARVGELGARGWKAWMHQQERGGWLQPSADDDEEEDEENEEEVKDPSQPRGTVWLDVESSREAAHWLPWRPDKAKGQSEEDCEDPDRQVLFDDIGPSLICLSSPELQLRLLLHFLSFLGLPVDSVLSPPPCLSGLLLESLSLLTQVNEFQRPLTSHNLPDRGVNSVGHMTTLQGTRKMVGLGKQGERFVTNLLNMVQPILPPHHRAVLSLSWMQYEKLKVFHCLRTGNKKHLRSQGKSSKRVAKRLLKEPDNRSSLVLWREYAHLEWMLGNLDEGRKVFSTATALGGTKGLSSPTLCELCQLWSQLEVEDGAPMQGGGLTDVTASPAVVVLTQLAEGTSSSSSSHSLSPVSILKARRSYEQALTASLSALDQVCSSPQTTRKGDEDLLGGKLRLSGLVGCYALFQYLTVGIQAATAVYTQAREKMDNLHHTLALDKPFNSNKETHHTSTDTKNSAADSSQISRRNVSKVAFECQILAVQQAALLRYHNSICVFPLATLRQMLTSALTTWSSSAPLWSIYVQVENRYHSAGRARRFFHSVTRETSSVVPRLFAIVAEQQRKQLVDAAQRSCCHDAALPILPENGLSNRIRGLFEGAIATEMGAHCPLLWRMYMHFLVSEGKVDKAKGIFYKALQSIPWVKGLYMDAVQLFPEHLQEFVDLMTEKELRVRLPLEELDILLED"

     misc_feature    420..731
                     /gene="nrde2"
                     /note="MTR4-interacting domain (MID) found in nuclear
                     exosome regulator NRDE2 and similar proteins; Region:
                     NRDE2_MID; cd22200"
                     /db_xref="CDD:412062"
     misc_feature    order(420..446,453..458,468..473,477..479,483..527,
                     534..542,546..554,588..596,606..611,615..620,627..647,
                     669..680,702..731)
                     /gene="nrde2"
                     /note="MTR4 binding site [polypeptide binding]; other
                     site"
                     /db_xref="CDD:412062"
     misc_feature    921..1886
                     /gene="nrde2"
                     /note="necessary for RNA interference; Region: NRDE-2;
                     pfam08424"
                     /db_xref="CDD:462472"
ORIGIN      

tatatgcgacgttcgccatggctctgtttccagcgtttgcagagacatcgagtaataaagttgaagattcgtccaaagactggctgaacaacaagagttttcagagcacagaagccctttctctccacaagcggtttttagagaacaaggagccaagtgttgacaaagaggtgagctcagctgaggagaagagggaagaagatgtgccccccaaaaagaaaaaaaagaagagtggaaagaggaaaaagaagaaaaaacacaaaaagaagagtggggggcattcagatagcagtgggtctgactctgacaccatctacccaagtgatctcaaaagagaagaggaggctaaaaagtcacaggctgctccgttagcaagttgtttctcctggttggatgaccttcagtcacccacagagcatccattctgcgttgaccgaaaaccagatccagccaactggacatacaagtccctttacagaggagatgtagctaggtataagaggaaaggcagatcatcattaggtctggatcctgtcagacagggagtcagctgggaggagtcagagtcaaataaaaaacagaagagtggggagaagaagaaaggagacaacagatatttctctagactcagccgccagatactgagatccaatgctgctgttcctacgttaccaacagttcctgaatgtagtgatgtcatcagcacctcctccttcttactgctgggtgatgatgaaggggcgaacaaagaaaaagagactagtgacagagtgcagacatcctctgtaaatccactcggtgtatatgactcttcaacagccctttggctgcaggggaagggacagcaggacaagacagaggagcagaaccaggacatagaggcaggacagggcgccttgcttatggccagaaggactgaagagtttaacaggcagctcagagagcagccagcagacacacagctatggataaaattcatacgataccaggatgaggtgagtttagcagagtttggaagtgaggagatgcaacaggaccacgattcaactgagcgtcgcaaatcctcctaccgagcagtcctggaaaaaaagctgagcattgctgaacgcgctgtggacaccaatcccacctgcatagctctgcagctggagaggctcaggatctgccaggagctgtgggagccctctgttctggctagagaatggaagaaactggtgttcctccacccaaatagtgcccccctatggagggagtatctgctcttcactcagagctacttcagcaacttcactgtgccaaaagtcaactctgcctatggaaagtgtctaagcacactcagcgctgtgcgggacggcagcatggtctctcatccagccctgccagggattgaggaggatatgctggatattttcatccagcagtgtcatttcctgcgccagtgtggacattcagagaaagcagtctctctatttcaggccatgatggattttactttctacaaacctgacagtgtacgagaaatgtccaccaagcagcaggctgagttttttgagccgttctgggacagtggggaggcgagggttggggagttgggggcaagaggctggaaagcctggatgcaccaacaagagcgtggaggatggctgcaacccagtgcagatgatgatgaggaagaggatgaagagaatgaggaagaggtgaaggatccaagccaaccgagaggaacagtctggctggatgtggagtcatcccgggaagcagcacactggttgccgtggagacctgacaaagctaagggccaatcagaagaggactgtgaggacccagatagacaggtgttatttgatgacatcggtccatccttaatttgcttatcctcaccagagctccaacttcgccttcttctccatttcttatcatttctggggctgcccgtggactctgtgctctctcctcccccctgcctgtctggcctgctgctggagagcctgtctctgctcactcaggtgaatgagttccagcgtccactgacctcccacaatctacctgaccgtggggttaactctgttggtcacatgaccactcttcagggaaccaggaaaatggtggggcttggaaaacagggtgagaggtttgtcacaaatttgttgaatatggtccaacctatccttcctcctcaccaccgagcagttctgtccctaagctggatgcagtatgaaaaactcaaggtctttcactgcttgcgcactggcaacaaaaagcatctgcgatctcaaggcaagagcagcaagcgagtggccaagcgacttctcaaggagcctgacaaccgctcttcgttggtgctgtggcgagagtatgcccacctggagtggatgctgggaaaccttgacgaaggtcgcaaagtcttctccacagccacagcattgggaggaaccaaagggctgagcagccccacactctgtgagctgtgtcagctgtggtcccagctggaggtggaggatggagcacccatgcaaggaggaggactaactgatgtaaccgcgtccccagctgtggtggtattaacccagctagcagaaggaacttcctcatcctcttcctcccattccctctctccagtatctatccttaaagccaggaggtcttatgaacaagctttgacagccagcttgtcagcacttgaccaagtctgcagcagtccgcagaccaccagaaaaggtgatgaagacctccttggaggcaaactgaggctgagtggcctggtaggttgctacgctctgttccagtacctcacagtgggaatccaagccgctactgctgtgtacacccaggccagagaaaagatggacaacctgcatcatacgctggcacttgacaagccatttaacagcaataaagaaactcaccacacaagcactgatacaaaaaactcagcagctgattccagccaaatcagcagacgcaatgtcagtaaagtagcctttgagtgtcagatactagcagtacaacaggcagccttactgaggtaccacaacagtatctgtgtctttccactggcaacattgagacaaatgctgacctctgccctcacaacttggtccagcagtgcccccctctggagcatttatgtacaggtagagaatcgttaccacagcgctggccgggcccgccgcttctttcactctgtaaccagggaaaccagcagtgtggtgccacgcctctttgccattgttgcggaacagcaaaggaagcagctggtggatgctgctcagaggtcttgttgccatgacgccgccctgcccatcctaccagagaatggcctcagcaaccgtatccgtggtctttttgaaggcgctatagcaacagagatgggtgctcactgtcctttgctttggaggatgtacatgcatttcctggtctcagaagggaaggtggataaagccaaaggaattttctataaggccttacagagtattccctgggtgaagggtttgtacatggatgccgtacagctgttccctgagcatctgcaagagtttgtagatctgatgacagagaaagaactccgagtgagactgcctttagaagaactggatatactactggaagactgaccacacacctagctgctgtaatgaatagtgaagttggaatgactttacctaaagatccacaaggggccattcctttgtagatgtctgaaaaggctttattttcacagtaaatagcaacatgttttattacttgataatgtcaataaatgggattcttatttgtt

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