LOCUS       XM_028963589            2769 bp    mRNA    linear   VRT 02-JUL-2025
DEFINITION  PREDICTED: Denticeps clupeoides argonaute RISC component 4 (ago4),
            transcript variant X2, mRNA.
ACCESSION   XM_028963589
VERSION     XM_028963589.1
DBLINK      BioProject: PRJNA533386
KEYWORDS    RefSeq.
SOURCE      Denticeps clupeoides (denticle herring)
  ORGANISM  Denticeps clupeoides
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Clupei; Clupeiformes;
            Denticipitoidei; Denticipitidae; Denticeps.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_041726.1) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Updated annotation
            Annotation Name             :: GCF_900700375.1-RS_2025_06
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.4
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 06/27/2025
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2769
                     /organism="Denticeps clupeoides"
                     /mol_type="mRNA"
                     /db_xref="taxon:299321"
                     /chromosome="20"
     gene            1..2769
                     /gene="ago4"
                     /note="argonaute RISC component 4; Derived by automated
                     computational analysis using gene prediction method:
                     Gnomon. Supporting evidence includes similarity to: 32
                     Proteins, and 100% coverage of the annotated genomic
                     feature by RNAseq alignments, including 1 sample with
                     support for all annotated introns"
                     /db_xref="GeneID:114770010"
     CDS             160..2751
                     /gene="ago4"
                     /codon_start=1
                     /product="protein argonaute-4 isoform X2"
                     /protein_id="XP_028819422.1"
                     /db_xref="GeneID:114770010"
                     /translation="

MEALGPGPPAPTSLFQPPRRPGLGTVGKPIRLLANHFQVQIPKIDVYHYDIDIKPEKRPRRVNREVVDTMVRHFKMQIFGDRQPGYDGKRNMYTAHPLPIGRDRVDLEVTLPGEGKDQTFRVSLQWVSVVSLQMLLEALSGHLNEVPEDSVQALDVITRHLPSMRYTPVGRSFFSPPEGYYHPLGGGREVWFGFHQSVRPAMWNMMLNIDVSATAFYRAQPVIEFMCEVLDIQNINEQTKPLTDSQRVKFTKEIRGLKVEVTHCGQMKRKYRVCNVTRRPASHQTFPLQLENGQAMECTVAQYFKQKYSLQLKYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIKATARSAPDRQEEISRLVKSNSMVGGPDPYLKEFGIVVHNDMTEVTGRVLPAPMLQYGGRNKTVATPNQGVWDMRGKQFYAGIEIKVWAVACFAPQKQCREDLLKSFTDQLRKISKDAGMPIQGQPCFCKYAQGADSVEPMFKHLKMSYVGLQLIVVILPGKTPVYAEVKRVGDTLLGMATQCVQVKNVVKTSPQTLSNLCLKINAKLGGINNVLVPHQRPSVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDGHPSRYCATVRVQTSRQDLSQEQLYSQEVIQDLTNMVRELLIQFYKSTRFKPTRIIYYRGGVSEGQMKQVAWPELIAIRKACISLEEDYRPGITYIVVQKRHHTRLFCSDKAERVGKSGNVPAGTTVDSTITHPSEFDFYLCSHAGIQGTSRPSHYHVLWDDNCFTADELQLLTYQLCHTYVRCTRSVSIPAPAYYARLVAFRARYHLVDKDHDSAEGSHVSGQSNGRDPQALAKAVQIHYDTQHTMYFA"

     misc_feature    241..630
                     /gene="ago4"
                     /note="N-terminal domain of argonaute; Region: ArgoN;
                     pfam16486"
                     /db_xref="CDD:465134"
     misc_feature    661..813
                     /gene="ago4"
                     /note="Argonaute linker 1 domain; Region: ArgoL1;
                     pfam08699"
                     /db_xref="CDD:462567"
     misc_feature    814..1176
                     /gene="ago4"
                     /note="PAZ domain, argonaute_like subfamily. Argonaute is
                     part of the RNA-induced silencing complex (RISC), and is
                     an endonuclease that plays a key role in the RNA
                     interference pathway. The PAZ domain has been named after
                     the proteins Piwi,Argonaut, and Zwille; Region:
                     PAZ_argonaute_like; cd02846"
                     /db_xref="CDD:239212"
     misc_feature    order(970..972,1015..1017,1057..1059,1069..1071,
                     1123..1125,1144..1146,1150..1152)
                     /gene="ago4"
                     /note="nucleic acid-binding interface [nucleotide
                     binding]; other site"
                     /db_xref="CDD:239212"
     misc_feature    1315..2622
                     /gene="ago4"
                     /note="PIWI domain, Argonaute-like subfamily. Argonaute is
                     the central component of the RNA-induced silencing complex
                     (RISC) and related complexes. The PIWI domain is the
                     C-terminal portion of Argonaute and consists of two
                     subdomains, one of which provides the...; Region:
                     Piwi_ago-like; cd04657"
                     /db_xref="CDD:240015"
     misc_feature    order(1726..1728,1738..1740,1774..1785,1792..1794,
                     1816..1818,1825..1827,1837..1839,1849..1851)
                     /gene="ago4"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240015"
     misc_feature    order(1930..1932,1936..1938,2176..2178,2590..2592)
                     /gene="ago4"
                     /note="active site"
                     /db_xref="CDD:240015"
ORIGIN      

atcacacagaagacagcgcaccctcgacggtccgcggacagctacgcacctctcccgtttctcaaattagcacccatcagcttcgcgataccgcccccaatttcagtgtcggtacggagccgccaggccgggacagagacggagagagagccctcggccatggaagcgctcggacccggcccgcctgctcctacctcactcttccagccaccacgtcggcccggcctgggcaccgtggggaagccgattcgcctgctggccaatcacttccaagtgcagatccccaagatcgacgtctaccattatgacatcgacatcaagcctgagaagcggccaaggagggtcaacagagaggtggttgacaccatggtcagacacttcaagatgcagatctttggagaccggcagcctggatatgatggcaagaggaacatgtacacggctcatccactacccattggaagagacagggtcgacctggaagtcacgcttccaggcgaagggaaggaccagaccttcagggtgtctctgcagtgggtgtcggtggttagtctgcagatgctcctggaagccctctccggtcacctgaacgaagttcccgaggactccgtgcaggcgctggatgtcatcacccgtcacctgccttccatgaggtatactccagtcggccggtccttcttctccccacccgagggctactatcatccgctcggtgggggcagagaggtgtggtttgggttccaccagtccgtccgtccagctatgtggaacatgatgctcaatattgacgtttcagcaacagctttctaccgtgcccagcctgtgatcgagtttatgtgtgaggtcctggacatccagaacatcaacgaacagaccaagcccctcaccgactcgcagcgtgtcaagttcaccaaggagatccgaggacttaaagtggaagttacgcactgtggtcagatgaaaaggaagtaccgggtctgcaatgtcacacgccgcccagccagccaccagacgttccccctccaactagaaaacggccaggccatggagtgcaccgtggcccagtacttcaaacagaagtacagcctgcagctcaaatatccacaccttccctgcctgcaggtggggcaggaacagaagcacacctacctgcccctggaggtatgtaacatagtggccggacagcgctgtataaagaagctcaccgataatcagacctcaacgatgatcaaagccacggcccgctctgcacctgatcggcaggaggagatcagtaggctggtcaaaagcaacagcatggtgggcggacccgacccctacctgaaagagtttggcatcgttgtgcataatgatatgactgaggtgactgggcgggtcctcccagcgcccatgctgcagtatgggggccggaacaagacggtggccacgcccaaccagggtgtgtgggacatgagggggaagcagttctacgccggcatcgagataaaggtgtgggccgtggcctgctttgcccctcagaagcaatgccgagaggacctactcaagagctttacggaccagctgaggaagatctcaaaggatgctgggatgccgatccaagggcagccctgcttctgcaagtacgcccaaggagccgacagcgtggagcccatgttcaagcacctcaagatgtcttatgtggggctgcagctgattgtggtgattttgccaggaaaaacaccagtgtatgcggaggtgaagcgtgtgggagacacgctgttgggcatggccactcagtgtgtgcaggtgaagaacgtggtgaagacgtcgccccagaccctgtccaacctctgcctcaagatcaacgccaagctgggcggcatcaacaacgtgctagtgccccaccagaggccttccgtgttccagcagccggtcatcttccttggtgcagacgtcacgcacccgccagcaggcgacgggaagaagccgtccatcgcggcggtggtgggcagcatggacggccaccccagccgctactgcgccacggtgcgcgtgcagacgtctcggcaggacctgtcgcaggagcagctctacagccaggaggtcattcaggatctgaccaacatggtgcgcgagctgcttattcagttctacaagtccacccgcttcaagcccacgcgcatcatctactaccgcgggggcgtgtccgagggccagatgaaacaggttgcgtggcccgagctcatagccatcagaaaggcctgtatcagcctggaggaggactaccggcctggcatcacttacatcgtagtgcagaagcgtcatcacaccaggctcttctgctccgacaaggctgagagggttggaaagagcggcaatgttccagcgggtaccaccgtggacagcaccatcacacacccatctgagtttgatttttacctgtgcagccatgctggtattcaaggcaccagtcggccctcacactaccacgtcctgtgggatgacaactgcttcacagcggacgagctgcaactgctcacctaccaactgtgccacacctacgtgcgctgcactcgctccgtttcaatcccagcgccagcttactacgctcgtctggtagccttccgtgcacgttaccatctggtggacaaagaccatgacagtgcggagggcagccatgtgtcaggccagagtaacggccgagaccctcaggctctggcgaaggcagttcagattcactatgacacccagcacaccatgtacttcgcctgacccaagccagcaggaggc

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