LOCUS       XM_028438780            3768 bp    mRNA    linear   VRT 30-JUN-2025
DEFINITION  PREDICTED: Gouania willdenowi NRDE-2, necessary for RNA
            interference, domain containing (nrde2), mRNA.
ACCESSION   XM_028438780
VERSION     XM_028438780.1
DBLINK      BioProject: PRJNA527273
KEYWORDS    RefSeq.
SOURCE      Gouania willdenowi (blunt-snouted clingfish)
  ORGANISM  Gouania willdenowi
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Ovalentaria; Blenniimorphae; Blenniiformes;
            Gobiesocoidei; Gobiesocidae; Gobiesocinae; Gouania.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_041065.1) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Updated annotation
            Annotation Name             :: GCF_900634775.1-RS_2025_06
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.4
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 06/27/2025
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3768
                     /organism="Gouania willdenowi"
                     /mol_type="mRNA"
                     /db_xref="taxon:441366"
                     /chromosome="22"
     gene            1..3768
                     /gene="nrde2"
                     /note="NRDE-2, necessary for RNA interference, domain
                     containing; Derived by automated computational analysis
                     using gene prediction method: Gnomon. Supporting evidence
                     includes similarity to: 4 Proteins, and 100% coverage of
                     the annotated genomic feature by RNAseq alignments,
                     including 3 samples with support for all annotated
                     introns"
                     /db_xref="GeneID:114456798"
     CDS             42..3620
                     /gene="nrde2"
                     /codon_start=1
                     /product="protein NRDE2 homolog"
                     /protein_id="XP_028294581.1"
                     /db_xref="GeneID:114456798"
                     /translation="

MQGVIMALFPAFAEEGGQKLEKPPKDLDWLNNKSFQIGDALSLHSRFLENTSEQLNNRQARDYDSTEEESGDTTHRRKKSEKKKKKKHKKKSRRHSESSASIFPSDLKREEETKSQQDALLASRFCWLEDFLSSTEQPFCVDRKPDPANWTYKSLYRGDVARYKRKGSSSLGVDARTQEISWEESDTKKKQKGRDSKRAADRYFSTVCRLLLRSELPVPVLPGCPAPNEASALSSASFLPLIDNEGERKGGQTGDSVQRSSVDPLNVYDSSTSLWRQGKGQQDQQIPDTHTNRESFGLMTGRTEEFNRQLREQPTDTQLWIQFIRYQDELNAAVFGGEEEQTGGESAERRRSSYRVVLEKKLSIAERAINANPVCIELQLERLWICQELWEPAVLAKEWKKLVFLHPNSAPLWREYLLFTQSYFSNFTVSKVNSAYGKCLSTLSAVRDGSMLSHPALPRTEEEMLDIFVQQCHFLRQSGHSEKAVSLFQAMIDFTFFKPDSVRKLTTKQQVEFFEPFWDSGEARVGEFGAKGWKTWMHQQERGGWLQPNAEEEEEEEDEDEEEVKDRSQPRWRVWLQVESTREFAHWLPWRPDKAKGQSEEDCEDPDRQVLFDDIGPSLICLSSAELQLCLLLHFLSFLGLPVDPVLSGIPCLPGLLLENLCLLTQGNKPQHPLTWYDLPEHGADSVGHMTTLQGARKCAGLGKMGEKFVTNVLNMILAVLPAHHKGVLSLSWIQYEKLKVQRYLCNSNKKCLRTQGKRSKQVVKRLLKEPENRSSLVLWREYAHLEWMLGNQDEARKVLSTASALDGSKGLSSRALCELCLLWSQLEVEKAEAERDGRHIDVTASPAVCILTRLTEGTSSFSSSTQTLSPVSILKARRSYEQSLIANLSALENLCGLQSREKGIEDVLRLRSLVGCYALFQYLTVNIQAANAVYTQARDRVDALYHTLTLEQKQGSSSEARRFAADPSSTSAPQHSIRRLASECEALAVQQACLLRHHNTVSVFPLSMLRETLTSSLVTCSSSAPLWSIYLQVENRYHSAGRARRFFHSVSRDNSSIVPRLFAIVAEQQRKQLVDAAQRSCCHDDALPMLPENGLSNRIRGLFESTSVTKMGAHCPLLWRMYVYFLVSEGKTDKAKGIFYKALQNIPWVKGLYMDAVQLFPENPQEFVDLMTEKEIRLRLPLEELDILLED"

     misc_feature    453..770
                     /gene="nrde2"
                     /note="MTR4-interacting domain (MID) found in nuclear
                     exosome regulator NRDE2 and similar proteins; Region:
                     NRDE2_MID; cd22200"
                     /db_xref="CDD:412062"
     misc_feature    order(453..479,486..491,501..506,510..512,516..560,
                     567..575,579..587,606..614,624..626,642..644,648..653,
                     660..680,711..722,741..770)
                     /gene="nrde2"
                     /note="MTR4 binding site [polypeptide binding]; other
                     site"
                     /db_xref="CDD:412062"
     misc_feature    954..1919
                     /gene="nrde2"
                     /note="necessary for RNA interference; Region: NRDE-2;
                     pfam08424"
                     /db_xref="CDD:462472"
ORIGIN      

ccattgtagtgacgagtgacgtcacactcctgcgactgtttatgcaaggcgtcatcatggcgttgtttccagcgtttgcagaagaagggggtcaaaaactcgagaaaccacccaaagatttagactggctgaacaataagagttttcagatcggggatgctctctctcttcacagtcgatttttagaaaacaccagtgagcagctgaacaacaggcaagcaagagattatgactcaactgaggaggagagtggagacacaacacacagaaggaagaagagtgaaaagaaaaagaagaagaaacataagaagaagagtaggaggcattctgagagcagtgcatccatcttccccagtgatcttaaaagggaggaagagacaaaaagccaacaagatgctctgttagcaagtcgtttctgttggttggaggatttcctgtcatctacagagcagcctttctgtgtggaccggaaacctgaccctgctaactggacttacaagtctctgtacagaggagacgtagcaagatacaagaggaaaggcagctcatctttgggtgtggatgctcgcacacaggagatcagctgggaagagtcagacactaaaaagaaacaaaaaggcagggacagtaaaagagctgcagacagatacttttccactgtgtgtcggctgctgctaaggtcagagcttcctgtgccggtgttacctggatgccctgctcccaacgaggcctccgctctcagctccgcctccttcctccccctgattgacaatgagggggagagaaaaggaggacagactggggacagtgtgcagcgctcctcagtagaccccctcaacgtatacgactcatccacatcactctggcgacagggcaaagggcagcaggatcaacagatacctgacacacacacaaacagagagagcttcgggctgatgactggtagaacagaggagttcaacaggcagctcagagaacagcccacagacacacaactctggattcagttcatacgataccaggatgagctgaatgctgctgtgtttggaggagaggaggaacaaactggaggggaatcagctgaacgtcgtaggtcttcgtacagagtggtcctggagaaaaagctcagcattgcagagcgtgcaatcaacgccaaccctgtctgcatagaactgcagctggagagactttggatctgccaggagctctgggagcctgcagtcctcgctaaggagtggaagaaactggtgttcctccacccaaacagcgccccactgtggagagagtatctccttttcactcagagctacttcagtaacttcactgtgtccaaagtgaactctgcctacgggaagtgtctgagcaccttgagtgcagtgagagatggcagtatgctgtctcatcctgctctgcccaggacagaggaggagatgttagacatctttgtccagcagtgtcacttcctgcgccagtcaggtcactcggaaaaggctgtctctctatttcaagccatgattgactttacctttttcaaacctgacagtgttcggaaactgaccaccaagcagcaggttgaattctttgagccgttctgggacagtggggaagccagagtgggtgagtttggggccaaaggctggaagacctggatgcaccaacaagagcgaggagggtggctgcagccaaatgcagaagaagaagaggaggaggaggatgaagatgaagaggaggtgaaggaccggagtcagcctagatggagggtgtggctgcaagtggagtcaactcgtgaatttgctcactggttgccttggcggcccgacaaagctaagggccaatcagaggaggactgtgaggaccctgacagacaggtgctgtttgatgacatcggtccatccctgatatgtctgtcttcagccgagctccagctttgtcttctccttcatttcttgtctttcctgggtctgccggttgaccccgtgctctcagggattccttgtctgcctggcctgctcctggaaaacctttgcctgcttactcagggtaataagcctcagcaccctctgacttggtatgacctgccagagcatggcgccgactctgtaggccacatgactactctccaaggagcccgaaagtgcgctgggcttgggaaaatgggtgaaaagtttgtcaccaatgtgctcaatatgatcctggcagtccttcctgcccaccacaaaggcgttctgtcacttagctggatccagtatgagaagttgaaggttcagcgatatttgtgcaacagcaacaaaaagtgccttcgcactcagggaaagcgcagtaagcaagtggtgaagcggctgctcaaagagccggaaaaccgttcatctctggtgttgtggcgagagtacgcccacctggagtggatgctggggaaccaggatgaggctcgcaaagtattatccacagcatcagcattggacggtagcaaagggctgagcagccgcgccctctgtgagctctgcctgctgtggtctcagttagaggtggagaaagcagaagcagagcgagatggacgacacattgacgtgaccgcatctccagccgtgtgcattctgacaaggctgactgaaggaacttcttcattctcctcatcaacccagactctctctccagtctctatcctgaaagcacggaggtcctatgaacagtctctgattgccaatctgtcagctctagaaaacctctgcggactacagtccagagaaaaaggtatcgaggacgtgctgagattgaggagtctagttggctgttatgctctgttccagtatcttacagtaaacatccaagctgcaaatgctgtctacacacaggcgagagacagagtggatgctctgtaccacactctgactttagaacagaagcagggcagcagcagtgaagcacgccgctttgcagctgatcccagctccacctctgcaccacagcacagcattagaaggctggcctctgagtgtgaggccctggctgtgcagcaggcttgtcttctgcggcatcacaacactgtcagtgtatttccactgtcaatgctcagagaaacactgacatcttctctcgtaacctgttccagcagtgcccctctctggagcatttatttacaggtggagaatcgttaccatagtgctggcagggcacgcaggttttttcactcagtgtccagagacaacagcagcatagtgccacgcctctttgctattgttgctgaacaacaaaggaagcagttagtggatgctgctcagagatcctgttgccatgatgatgccctccccatgctgccagaaaatggcctcagcaaccgtattcgtggattgttcgaaagcacctcagtaacaaagatgggtgctcactgtccattactctggaggatgtacgtttactttctggtgtcagaaggaaaaacagacaaagccaaaggaatattctacaaagccttacagaatataccttgggtgaagggtttgtatatggatgcagtgcagctgtttccagagaacccgcaggagtttgtagatctgatgacagagaaagaaatccgtctgaggctgcctctggaggagttggatatacttttggaagactgaccacacacaactccttgaagaacaatccacacacacacaagaacatgggctcaaaattgcaaatattggagctaaaacactgagcacaagatgacagcaagcatattcatctgataattgtcaataaaaatgtgattttctgaagtaa

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