LOCUS       XM_028422496            6747 bp    mRNA    linear   VRT 15-JUL-2025
DEFINITION  PREDICTED: Parambassis ranga argonaute RISC component 4 (ago4),
            transcript variant X5, mRNA.
ACCESSION   XM_028422496
VERSION     XM_028422496.1
DBLINK      BioProject: PRJNA526690
KEYWORDS    RefSeq.
SOURCE      Parambassis ranga (Indian glassy fish)
  ORGANISM  Parambassis ranga
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Ovalentaria; Ambassidae; Parambassis.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_041023.1) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Updated annotation
            Annotation Name             :: GCF_900634625.1-RS_2025_07
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.4
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 07/15/2025
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6747
                     /organism="Parambassis ranga"
                     /mol_type="mRNA"
                     /db_xref="taxon:210632"
                     /chromosome="2"
     gene            1..6747
                     /gene="ago4"
                     /note="argonaute RISC component 4; Derived by automated
                     computational analysis using gene prediction method:
                     Gnomon. Supporting evidence includes similarity to: 2
                     Proteins, and 100% coverage of the annotated genomic
                     feature by RNAseq alignments, including 3 samples with
                     support for all annotated introns"
                     /db_xref="GeneID:114446704"
     CDS             167..2806
                     /gene="ago4"
                     /codon_start=1
                     /product="protein argonaute-4 isoform X5"
                     /protein_id="XP_028278297.1"
                     /db_xref="GeneID:114446704"
                     /translation="

MEALGPGPPAPTSLFQPPRRPGLGTVGKPIRLLANHFQVQIPKIDVYHYDIDIKPEKRPRRVNREVVDTMVRHFKMQIFGDRQPGYDGKRNMYTAHPLPIGRDRVDLEVTLPGEGKDQTFKVSLQWVSVVSLQMLLEALSGHLNEVPEDSVQALDVITRHLPSMRYTPVGRSFFSPPEGYYHPLGGGREVWFGFHQSVRPAMWNMMLNIDVSATAFYRAQPVIEFMCEVLDIQNINEQTKPLTDSQRVKFTKEIRGLKVEVTHCGQMKRKYRVCNVTRRPASHQTFPLQLENGQAMECTVAQYFKQKYNLQLKYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIKATARSAPDRQEEISRLVKSNSMVGGPDPYLKEFGIVVHNDMTEVTGRVLPAPMLQYGGRVSTDTGRDCGRGLSPQNKTVATPNQGVWDMRGKQFYAGIEIKVWAVACFAPQKQCREDLLKSFTDQLRKISKDAGMPIQGQPCFCKYAQGADSVEPMFKHLKMSYVGLQLIVVILPGKTPVYAEVKRVGDTLLGMATQCVQVKNVVKTSPQTLSNLCLKINAKLGGINNVLVPHQRPSVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDGHPSRYCATVRVQTSRQDMSQEQLFSQEVIQDLTNMVRELLIQFYKSTRFKPTRIIYYRGGVSEGQMKQVAWPELIAIRKACISLEEDYRPGITYIVVQKRHHTRLFCSDKAERVGKSGNVPAGTTVDSTITHPSEFDFYLCSHAGIQGTSRPSHYHVLWDDNCFTADELQLLTYQLCHTYVRCTRSVSIPAPAYYARLVAFRARYHLVDKDHDSAEGSHVSGQSNGRDPQALAKAVQIHYDTQHTMYFA"

     misc_feature    248..637
                     /gene="ago4"
                     /note="N-terminal domain of argonaute; Region: ArgoN;
                     pfam16486"
                     /db_xref="CDD:465134"
     misc_feature    668..820
                     /gene="ago4"
                     /note="Argonaute linker 1 domain; Region: ArgoL1;
                     pfam08699"
                     /db_xref="CDD:462567"
     misc_feature    821..1183
                     /gene="ago4"
                     /note="PAZ domain, argonaute_like subfamily. Argonaute is
                     part of the RNA-induced silencing complex (RISC), and is
                     an endonuclease that plays a key role in the RNA
                     interference pathway. The PAZ domain has been named after
                     the proteins Piwi,Argonaut, and Zwille; Region:
                     PAZ_argonaute_like; cd02846"
                     /db_xref="CDD:239212"
     misc_feature    order(977..979,1022..1024,1064..1066,1076..1078,
                     1130..1132,1151..1153,1157..1159)
                     /gene="ago4"
                     /note="nucleic acid-binding interface [nucleotide
                     binding]; other site"
                     /db_xref="CDD:239212"
     misc_feature    1322..2677
                     /gene="ago4"
                     /note="PIWI domain, Argonaute-like subfamily. Argonaute is
                     the central component of the RNA-induced silencing complex
                     (RISC) and related complexes. The PIWI domain is the
                     C-terminal portion of Argonaute and consists of two
                     subdomains, one of which provides the...; Region:
                     Piwi_ago-like; cd04657"
                     /db_xref="CDD:240015"
     misc_feature    order(1781..1783,1793..1795,1829..1840,1847..1849,
                     1871..1873,1880..1882,1892..1894,1904..1906)
                     /gene="ago4"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240015"
     misc_feature    order(1985..1987,1991..1993,2231..2233,2645..2647)
                     /gene="ago4"
                     /note="active site"
                     /db_xref="CDD:240015"
ORIGIN      

agcagcggtagttcaggtcagtcagtccagtcagcagtcatccacgcatctatctatattcccttttcttaaattagccactttaagcttcgcgataccgcccccaatttcagcgtcggtacggagccgccaggccgggacagagacggagagagaggcctcggccatggaagcgctcggaccaggcccgcctgcccctacctctctgtttcagcctcctcggcgtcccggccttggcacggtggggaaacccatccggctccttgccaaccacttccaggtgcagattcccaagattgatgtctatcactatgatatcgacatcaagcctgagaaacggcctcgaagggttaacagggaagtggtggataccatggtgcggcacttcaagatgcagatctttggagacagacagcctggttacgacgggaagaggaacatgtacacagcacatccactgccaatcgggagagacagggtggatctggaggtgaccctgcctggcgaggggaaagatcagacgttcaaggtgtctttgcagtgggtgtctgtggtcagtctccagatgctgctggaagccctgtcgggtcaccttaacgaggttccagaggactccgttcaggccctggatgtcatcacgcggcaccttccttccatgagatacactccagtggggcgttcgtttttctcccctccggagggttattaccatccgctcggtggaggcagggaggtgtggttcggtttccatcagtccgtccgtcctgccatgtggaacatgatgctcaacatcgatgtgtcggccactgctttctaccgtgctcagcctgtgatagagttcatgtgtgaggtgcttgacattcagaacatcaacgaacagaccaaaccactgacggactcgcagcgtgtcaaattcaccaaggaaataagaggcttgaaagttgaggtaacacactgtggtcagatgaagaggaaatatcgtgtgtgcaatgtcacgcgccgacctgccagtcaccaaacgttccccttgcagctagagaacggccaggctatggagtgcacagtagctcagtatttcaagcagaagtacaatctccagctcaaatatccacacttaccttgtctgcaagtgggacaagaacagaagcacacataccttcccctggaggtctgtaacattgtagcaggccagcgctgtatcaagaaactaacagacaaccagacatccaccatgattaaagctacagctcgctctgcccctgacagacaggaagagatcagcagactggtcaaaagtaacagcatggttgggggcccggatccctacctgaaggaatttggtattgttgtgcataatgacatgacagaagtaaccggtcgtgtcctcccagcgcccatgcttcagtatgggggccgggtgagtacagacacagggagggactgtggcaggggactctctccgcagaataaaaccgtggccacacccaaccagggcgtgtgggacatgagagggaagcagttctacgccggcatcgagatcaaggtctgggctgtggcctgcttcgctccacagaaacagtgtcgagaggacctgctcaagagtttcacagaccagctacgaaagatctcaaaggatgctgggatgcccattcaggggcagccatgtttctgtaaatatgcccaaggagctgacagcgtagagcccatgttcaaacaccttaagatgtcttatgtaggtctgcagctgattgtggtcatcctgcctggcaaaactccggtctacgctgaggtgaagcgggtgggtgacaccctcctcggcatggccacccagtgcgtgcaggtgaagaatgtggtgaagacgtccccccagacactctccaacctctgcctcaagatcaacgctaagctaggaggcatcaacaacgtcctggtgcctcaccagaggccttccgtgtttcagcagccggtcattttcctgggtgctgatgtcacacatcctcctgccggtgatgggaagaagccgtccatcgcagcggtggtgggcagcatggatggccaccccagccgttactgtgcaacagtgcgagtccaaacatcccgacaagacatgtcccaggagcagctcttcagtcaggaggttattcaagatttgaccaacatggtgcgggagctgctcattcagttctacaagtcgacccgttttaagcccacacgcatcatctactatcgtggcggtgtgtcggagggacagatgaagcaggtggcgtggccggagctcatagccatcaggaaggcgtgcatcagtctggaggaggattaccggccgggcattacttacatcgtagtccagaagcgccaccacactcgcctcttctgctctgacaaagctgagcgggttggaaagagcggtaatgttccagccggcaccacagtggacagcaccatcacacacccgtccgagttcgacttctacctgtgcagccatgctgggattcagggaaccagtcgtccctcccactaccacgtcctgtgggatgacaactgcttcacggccgatgaactgcagctgctcacttaccagctgtgccacacctatgtccgctgcacacggtctgtttccatcccggcgccggcctactacgccaggctagtggcttttcgagcccgctaccacctggtggacaaagaccacgacagtgctgaaggcagccatgtgtcgggccagagtaacggccgagacccacaggcattagcaaaggcagttcagatccactatgatacccagcacaccatgtactttgcctgagctaccaagcatgtccgccagtccacctgtggattcttctctctctctctctctatatatttttttttttttaaatctcttgtttgccagtttctcttcgtttcctcatctctctctctctctctatccttgtagtctatctcttctcgttttactcactcttcattttctgaatctgcaccaaagcggctcactgcggaggaagatgaggaggagggggggggggtcttctgtttcacggaacagtctccaaccagcgttcccaatcgagtcgccttttcaaccagcaatccagcactgcgaacccagagggtttccaatgcacaaaagaaaaagaaaaaaaaaagacgaaaaaacaaaaacaaaccatacacgacacgcatactacaagttgagccattattttgagtttgtttttgttttttctatttgaatgtctgcactcatgtgtttgtaagatacactgagcgagggagtggattggcacaagtcgtgtcagcgcagctctttagctctcccagctaagcaggactcttatctacttttacctcaaagcccctttgggcgaaatctgtcacaaggtcttgggtttatcgggtggtggggagggaactctacacgaacagagatgaaggggagctgaccttttgagaatgatatatatattatatatattttttttcctttttatgagcgtgtgttttaacttttctccttttcagtcttttacaagcgaggtgacctgggtgtccatgcccattgctactttcctcccatcttagcgaagcaagtctgcgtgtgtgcgtgtgtgtgtgtgtgtatgtgcgcgcatctctgtatatacatgtactgctgtatgagagagtgtttgtgtgtcgtttcatggcaggccactgaattgtaaagggaaatcaaggaaataactgctgtaaatgcctcagatttgttgtttttatattcacacataaaatacatataagcatatatttacatctatagagcatcttcgagacatatgacttaaaacaggcctcatagaggctggtgtgggtatctgtagcagtcctgggtcgctcgttcgctgaaatgctttgagttacatgtgagcatgtgcagtcgggtcaggtggtggtggtgggggggtgtttgaatccgagaaagtaacttgatgcatttcatgtcgtaaccgcagccaggtctgaagtgtagcagtagcaaccgtggtctaggcctgtgactggcttagcattgggacccgaaggcattaaacacaaagttctggtcttatcagccttttctatctgtgtggaaaagcaggatcttatttttttttctttggacctgattatgcgaaagattatatgatgacactgctggacaattcagtttttttagttgttttttttttttcctaggtctcctttgctaatattagccaactcattgcactgacccgggacgctaggcaagcaacagagggcactctcaatctctcttacctttatgcatttatacatacgaatcaacaaagcagatttgtaaaagtttaatataagatgttttgtggttatcttaaaaaaaaatactgttgtttagacttagtagtttaccattgatgttgttgttgttatcgttgttgtcaatgttgttactattgtgatgaaattttaaggttggcatcaaagccagttatatagttaactagtgtgacttcaggaatagacttcagttgttgagctgcaggttaaagaggggtagagctttgggttggatctttttttttttgtagcattctggttaacggtgagacgaagtttggccccaggatagatgatgatgatgatgatgatgatgattcttacacagtggtagatgtgtgtgctgctcactcggcagctagtttcgatcaaatggatggagacaatcaggactcgtgatcagagtgattaaagggattgtttttttttttaatataaaaaaaaaagcggcttgcgataggcagtgagttgaatttttagactttttaaaatggtttctattaactactctgtgtgtatttgaagtatacccatcttactatttaacagtgtttgtttcatatttttatgcatttttaacactggaattaaattgacaatacgcatcagcactgcccttggaccccacctctgttcaaaccaagtggcgccccctggtgttacagacaatgcaggacaggcgaggttggaagatgttgcgcacgcctcatcaggggggggacagctcagtgaaaactcttttgtaataaaccacccgtaaagcaacaggtttactatgaatgagtttgagcaccttcctgttgaaggcgtgagcgttggtgaaggatgtgggggaggcgggggaggagggggggggggtgtattccaggcattgcatgcaataagttttgtttttaaatgggggggcagatcagatttttaaaacgtgcactagtcttaccaagccaggctctgattgaatattcatctgccgtctcctaacgggaacaaaaatcccagtatttactcactgagctccagtcgcatcactccccgtttgtaacgcgacatcacgaagcaatattaacccagttcaagttttcaaatgtggaaaaacgaagctataattctactcattgtggttgttggtctactctcgctattgtgtgtctgtactttttattatttttttgaatttttatctctttggttcattttatttgcttgtatcaatcagtgggaatgtgcatttggtggaatagttatgtaggctttagacaattttcttttcaagtaacaaaatgtagactttgctcgcctgcatgccagagtattgtccttttaaacaaaaaaaaaaacaaaaaaaagaagaaaaaacctttttcaatcatatttattatgaggaaagaaaaaatataaacaccttttttggggggttggggtgggattgggtgggtaaatgtttatcaatttattgggactgtttttagcctagcaatatttgtccggtctctctattttgaaagattcctacacaaaaacgtcagtaatttggcaggtacaagctgtttatttgttttgttttgggcttttttttttttttttttttgaggggagggggggggggggctgcggctctttgcgttgcgttcgaattcaaacgaggtactttcttcaaacgtggacccgttaaagagccaaccaccccccccctttctcatcgttgccagggttactgttgcgagcatccattttgtctcctggttcctggtttttgtacctgtggccatgccttttggtttgcggctctcctgtttccatagcaactctgctgaccccttagcaaatgcacttcctctttgtggaggggtctctgcggcatcggcggagcaggattacctctttttaacctttttaaaaaaagatccaaaattctttctgtgccccaatagaaagagagcatgaattatttttaaattcctctcactttgatatattgttatatcttgacagatttgtaatttgacattgtgtattgtgtattttattttgttttccctctcttggttccttttttcgggggtttgttatagctctgctatgtttatttttttttttttttttgttttgttttctttccgctagtggaaactgtgtgtgtgattgtctgatagcaatagaaccacagcattggaacacacgccagttctgtgaatggagtgtcccgattctgtgaatgaacggaaccctttctgattggttgtaaagagggcgggggtgtgttgtagccccgtcctcagcagagggccattcactcgctgttattgctattatgtgacatgttcaaatacatttttttttttagcctgcagggaattcttgtgttaatgtgcaaagtaataaattggtattttatgcctataa

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