LOCUS       XM_023734735            3153 bp    mRNA    linear   MAM 29-JAN-2024
DEFINITION  PREDICTED: Trichechus manatus latirostris nuclear factor kappa B
            subunit 1 (NFKB1), transcript variant X3, mRNA.
ACCESSION   XM_023734735
VERSION     XM_023734735.1
DBLINK      BioProject: PRJNA189960
KEYWORDS    RefSeq.
SOURCE      Trichechus manatus latirostris (Florida manatee)
  ORGANISM  Trichechus manatus latirostris
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Afrotheria; Sirenia; Trichechidae; Trichechus.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_004444007.1) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Updated annotation
            Annotation Name             :: GCF_000243295.1-RS_2024_01
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.2
            Annotation Method           :: Best-placed RefSeq; Gnomon;
                                           cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 01/26/2024
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3153
                     /organism="Trichechus manatus latirostris"
                     /mol_type="mRNA"
                     /isolate="Lorelei"
                     /sub_species="latirostris"
                     /db_xref="taxon:127582"
                     /chromosome="Unknown"
                     /sex="female"
     gene            1..3153
                     /gene="NFKB1"
                     /note="nuclear factor kappa B subunit 1; Derived by
                     automated computational analysis using gene prediction
                     method: Gnomon. Supporting evidence includes similarity
                     to: 25 Proteins, and 100% coverage of the annotated
                     genomic feature by RNAseq alignments, including 7 samples
                     with support for all annotated introns"
                     /db_xref="GeneID:101351866"
     CDS             179..3091
                     /gene="NFKB1"
                     /codon_start=1
                     /product="nuclear factor NF-kappa-B p105 subunit isoform
                     X3"
                     /protein_id="XP_023590503.1"
                     /db_xref="GeneID:101351866"
                     /translation="

MAEDDPYLVGHEQMFHLEQLNHTIFNPELFQPEMPLPTADGPYLQILEQPKQRGFRFRYVCEGPSHGGLPGASSEKNKKSYPQVKICNYVGPAKVIVQLVTNGKNIHLHAHSLVGKHCEDGICTVTAGPKDMVVGFANLGILHVTKKKVFETLEARMTEACIRGYNPGLLVHPDLAYLQAEGGGDRQLTDREKEIIRQAALHQTKEMDLSVVRLMFTAFLPDSTGSFTRRLEPVVSDAIYDSKAPNASNLKIVRMDRTAGCVTGGEEIYLLCDKVQKDDIQIRFYEEEENGGVWEGFGDFSPTDVHRQFAIVFKTPKYKDINITKPASVFVQLRRKSDLETSEPKPFLYYPEIKDKEEVQRKRQKLMPNFSDSFGGGSGAGAGGGGMFGSGGGGGGTGSTGPGYGFPPYGFPTYGGIAFHSGTTKSNAGMKHGDIGTPSKKDHKGCDDRDAINLSGKVTETTEQDKESSSEHEEVTLTYTVGVKEENCRFQDNLFLEKAMQLAKRHANALFDYAVTGDVKMLLAVQRHLTAVQDENGDSVLHLAIIHLHAQLVRDLLEVTAGVISDDIINMRNDLYQTPLHLAVITSQEDVVEDLLRAGADLSLLDRLGNSVLHLAAKEGHDKILSILLKHKKAALLINQPNGEGLNAIHIAMMSNSMSCLRLLMAAGADIDAQEQKSGRTALHLAVEHDNISLAGCLLLEGDAHVDSTTFDGTTPLHIAAGRGSTRLAALLKAAGADPLVENFEPLYDLDDSWERDGEDEGVVPGTTPLDMAANWQVFDILNGKPYESELTSDDLLAQGDMKQLAEDAKLQLYKLLEIPDPDKNWATLAQKLGLGILNNAFRLSPAPSKTLMDNYEVSGGTVKELVEALRQMGYTEAIEVIQTAFCSSGTATTSSVKTPSQAHSLPLSPSSTRQQIDELRDNESICDSGVETSFRKLSFTESLTSSGSLLTLNKMPHDYGQHGSIEGNV"

     misc_feature    302..907
                     /gene="NFKB1"
                     /note="N-terminal sub-domain of the Rel homology domain
                     (RHD) of nuclear factor of kappa B1 (NF-kappa B1); Region:
                     RHD-n_NFkB1; cd07935"
                     /db_xref="CDD:143651"
     misc_feature    order(344..346,350..355,359..364,371..382,605..607,
                     611..616,905..907)
                     /gene="NFKB1"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:143651"
     misc_feature    926..1231
                     /gene="NFKB1"
                     /note="IPT domain of the transcription factor NFkappaB and
                     related transcription factors. NFkappaB is considered a
                     central regulator of stress responses, activated by
                     different stressful conditions, including physical stress,
                     oxidative stress, and exposure to...; Region:
                     IPT_NFkappaB; cd01177"
                     /db_xref="CDD:238582"
     misc_feature    order(929..931,935..943,947..955,1073..1081,1118..1120,
                     1154..1156,1211..1213,1220..1222,1226..1228)
                     /gene="NFKB1"
                     /note="ankyrin protein binding site [polypeptide binding];
                     other site"
                     /db_xref="CDD:238582"
     misc_feature    order(935..940,944..946,983..985,989..991,995..997,
                     1094..1099,1106..1108,1112..1114)
                     /gene="NFKB1"
                     /note="dimerization interface [polypeptide binding]; other
                     site"
                     /db_xref="CDD:238582"
     misc_feature    order(998..1000,1004..1006,1097..1102)
                     /gene="NFKB1"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:238582"
     misc_feature    1646..2467
                     /gene="NFKB1"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     Region: ANKYR; COG0666"
                     /db_xref="CDD:440430"
     misc_feature    order(1784..1786,1790..1792,1802..1807,1814..1822,
                     1826..1831,1841..1843,1850..1852,1895..1897,1901..1903,
                     1907..1909,1919..1924,1931..1939,1943..1948,1958..1960,
                     1967..1969,1994..1996,2000..2002,2006..2008,2018..2023,
                     2030..2038,2042..2047,2057..2059,2066..2068)
                     /gene="NFKB1"
                     /note="oligomer interface [polypeptide binding]; other
                     site"
                     /db_xref="CDD:293786"
     misc_feature    1784..1897
                     /gene="NFKB1"
                     /note="ANK repeat [structural motif]; Region: ANK repeat"
                     /db_xref="CDD:293786"
     misc_feature    1901..1996
                     /gene="NFKB1"
                     /note="ANK repeat [structural motif]; Region: ANK repeat"
                     /db_xref="CDD:293786"
     misc_feature    2108..2200
                     /gene="NFKB1"
                     /note="ANK repeat [structural motif]; Region: ANK repeat"
                     /db_xref="CDD:293786"
     misc_feature    2210..2308
                     /gene="NFKB1"
                     /note="ANK repeat [structural motif]; Region: ANK repeat"
                     /db_xref="CDD:293786"
     misc_feature    2606..2827
                     /gene="NFKB1"
                     /note="Death domain of the Nuclear Factor-KappaB1
                     precursor protein p105; Region: Death_NFkB1_p105; cd08797"
                     /db_xref="CDD:260063"
ORIGIN      

cgcggcgctgactggcccggcccggcgataccgcgcgccctcccgctgcgacccgccctgcacccggacggcttccagctccggcctcttcctccccgagcccccaggcccgcgccgccccggagggggagcccacaggcaccgtgaggccagcgcagactcgcgaccaggctccagaatggcagaagacgatccatatttggtagggcatgaacaaatgtttcacttggagcaattgaatcatacgatatttaatccagaattatttcaaccagagatgccactgccaacagcagatggcccataccttcaaatattagaacaacctaaacagagaggattccgtttccgttatgtatgtgaaggcccatcccatggtggccttcctggtgcatctagtgaaaagaacaagaagtcttaccctcaagtcaaaatctgcaactatgtgggacctgcaaaggttattgttcagctggtcacaaatggaaaaaacatccacctgcatgcgcacagcctggtggggaagcactgtgaggatgggatctgcactgtgactgctggacccaaggacatggtggtcggctttgcaaacctgggtatacttcatgtgacaaagaaaaaagtatttgagacactggaagcacgaatgacagaggcatgtataagaggctataaccctggacttttagtgcatcctgaccttgcctatttgcaagcagaaggtggaggagacaggcaactcacagatcgggaaaaggagataatccgccaggccgctcttcatcagaccaaagagatggacctcagcgtggtgcggctcatgtttacagcttttcttccagacagcacgggcagcttcacaaggcgtttggagcccgtggtctcagatgccatctatgacagcaaagcccccaatgcgtccaatttgaaaattgtaagaatggacaggacagccggatgtgtaactggaggggaagagatttatcttctctgtgacaaggttcagaaagatgacatacagattcgattttatgaagaggaggaaaatggtggagtctgggaaggatttggagatttttcacccacagatgttcataggcagtttgccattgtcttcaaaaccccaaagtataaagatatcaacattacaaaaccagcctctgtgttcgtccagcttcgaaggaaatctgatttggaaactagtgaaccaaaaccttttctctactaccctgaaatcaaagataaagaggaagtgcagaggaaacggcagaagctcatgcccaatttttcggatagtttcggtggtggtagtggtgctggagctggaggcggaggcatgttcggtagtggcggtggaggagggggtactggaagtacaggcccagggtatggcttccccccctacggatttcctacatatggtggaatcgccttccattctggaaccactaaatctaatgctgggatgaagcatggagacataggcaccccatctaaaaaagaccataaaggttgtgatgacagagatgctataaatctttctgggaaggtaactgaaaccacagagcaagataaggagtccagcagtgagcatgaagaggttactctgacgtatacagtaggagtaaaggaagagaattgcaggtttcaggataacctctttctagagaaggctatgcagcttgccaagcggcacgctaatgccctttttgactacgcggtaacaggagatgtgaagatgctgctggctgtccagcgccatcttactgcagtgcaggatgagaatggggacagcgtcttacacttagcgatcatccaccttcatgctcagcttgtgagggatctgctagaagtcacagcaggtgtgatttctgatgacatcatcaacatgagaaatgatctttaccagacgcccctgcacttagcagtaatcaccagtcaagaagatgtggtggaggacttgctgagggccggggctgacctgagtcttctggaccgcttgggtaactctgttttacacctagctgccaaagaaggacatgataaaattctcagtatcttactcaagcacaaaaaggcagcactacttatcaaccagcccaacggggaaggtttgaacgccattcacatagccatgatgagcaacagcatgtcgtgtctgcggctgctgatggccgccggcgcagacatagatgctcaggagcagaagtctgggcgcacggcactgcacctggctgtggagcatgacaacatctccttggctggctgcctgcttctggagggtgatgcccatgtggacagtactacctttgatggaactacacccttgcacatagcagctgggagagggtccaccaggctggcagctcttctcaaagcagcaggagcagatccactggtggagaactttgagcctctctatgacctggatgactcttgggaaagggatggagaggacgaaggggtcgtgcctggaaccacacctttagatatggctgccaactggcaggtttttgacatattaaatgggaaaccatatgagtcagagttaacatctgatgatttactggcacaaggagacatgaaacagctggctgaagatgcaaagttgcagctctacaagttgctagaaatccctgatccagacaaaaactgggccactctggcacagaaattaggtctgggtatacttaataatgctttccgactgagtcctgctccttctaaaactcttatggacaactacgaggtctctggagggacagtaaaggaactggtagaggccctgagacagatgggttacacggaagcaattgaagtcatccagacagccttctgcagctcaggaacagcaaccaccagctcagtgaagacgccttctcaggcccactcgctgcctctctcaccttcctctacacggcagcaaatagatgagctccgagataacgaaagcatctgtgacagcggcgtggagacctccttccgcaaactcagcttcacagagtctctgaccagcagcggctcattgctaactctgaacaaaatgccccacgattatgggcagcacggatctatagaaggcaatgtttaacctgctggtgattttcaacactgcaaaactgaagccctgaaattccaccacattgtccaaaa

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