LOCUS       XM_018878309            1221 bp    mRNA    linear   PLN 07-JAN-2024
DEFINITION  Sugiyamaella lignohabitans sphingosine N-acyltransferase LAG1
            (LAG1), partial mRNA.
ACCESSION   XM_018878309
VERSION     XM_018878309.1
DBLINK      BioProject: PRJNA342695
            BioSample: SAMN04417247
KEYWORDS    RefSeq.
SOURCE      Sugiyamaella lignohabitans
  ORGANISM  Sugiyamaella lignohabitans
            Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina;
            Dipodascomycetes; Dipodascales; Trichomonascaceae; Sugiyamaella.
REFERENCE   1  (bases 1 to 1221)
  AUTHORS   Bellasio,M., Peymann,A., Valli,M., Sipitzky,M., Graf,A., Sauer,M.,
            Marx,H. and Mattanovich,D.
  TITLE     Complete genome sequence and transcriptome regulation of the
            pentose utilising yeast Sugiyamaella lignohabitans
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1221)
  CONSRTM   NCBI Genome Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2024) National Center for Biotechnology
            Information, NIH, Bethesda, MD 20894, USA
REFERENCE   3  (bases 1 to 1221)
  AUTHORS   Peymann,A. and Graf,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2016) Department of Biotechnology, University of
            Natural Resources and Life Sciences, Muthgasse 18, Vienna, Vienna
            1190, Austria
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. This record is derived from an annotated genomic
            sequence (NC_031672).
            COMPLETENESS: incomplete on both ends.
FEATURES             Location/Qualifiers
     source          1..1221
                     /organism="Sugiyamaella lignohabitans"
                     /mol_type="mRNA"
                     /strain="CBS 10342"
                     /type_material="culture from holotype of Candida
                     lignohabitans"
                     /db_xref="taxon:796027"
                     /chromosome="A"
     gene            <1..>1221
                     /gene="LAG1"
                     /locus_tag="AWJ20_1430"
                     /db_xref="GeneID:30033232"
     CDS             1..1221
                     /gene="LAG1"
                     /locus_tag="AWJ20_1430"
                     /inference="similar to AA sequence:KEGG_Orthology:K04709"
                     /note="Ceramide synthase component; involved in synthesis
                     of ceramide from C26(acyl)-coenzyme A and
                     dihydrosphingosine or phytosphingosine, functionally
                     equivalent to Lac1p; forms ER foci upon DNA replication
                     stress; homolog of human CERS2, a tumor metastasis
                     suppressor gene whose silencing enahnces
                     invasion/metastasis of prostate cancer cells; LAG1 has a
                     paralog, LAC1, that arose from the whole genome
                     duplication; GO_component: GO:0061576 - acyl-CoA ceramide
                     synthase complex [Evidence IDA] [PMID 15692566];
                     GO_component: GO:0005783 - endoplasmic reticulum [Evidence
                     IEA]; GO_component: GO:0005783 - endoplasmic reticulum
                     [Evidence IDA] [PMID 11914276]; GO_component: GO:0005783 -
                     endoplasmic reticulum [Evidence IDA] [PMID 14562095];
                     GO_component: GO:0005783 - endoplasmic reticulum [Evidence
                     IDA] [PMID 22842922]; GO_component: GO:0005789 -
                     endoplasmic reticulum membrane [Evidence IEA];
                     GO_component: GO:0016021 - integral component of membrane
                     [Evidence IEA,IEA]; GO_component: GO:0016021 - integral
                     component of membrane [Evidence ISM] [PMID 12192589];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050291 - sphingosine N-acyltransferase
                     activity [Evidence IEA]; GO_function: GO:0050291 -
                     sphingosine N-acyltransferase activity [Evidence IMP]
                     [PMID 11387200]; GO_function: GO:0050291 - sphingosine
                     N-acyltransferase activity [Evidence IMP] [PMID 11694577];
                     GO_function: GO:0050291 - sphingosine N-acyltransferase
                     activity [Evidence IDA] [PMID 15692566]; GO_function:
                     GO:0016740 - transferase activity [Evidence IEA];
                     GO_process: GO:0046513 - ceramide biosynthetic process
                     [Evidence IMP] [PMID 11694577]; GO_process: GO:0046513 -
                     ceramide biosynthetic process [Evidence IDA] [PMID
                     15692566]; GO_process: GO:0006629 - lipid metabolic
                     process [Evidence IEA]; GO_process: GO:0001302 -
                     replicative cell aging [Evidence IEP,IMP] [PMID 8195187]"
                     /codon_start=1
                     /product="sphingosine N-acyltransferase LAG1"
                     /protein_id="XP_018735625.1"
                     /db_xref="GeneID:30033232"
                     /translation="

MARQRRTSSVGNIPLGDNAGPALSTMQPAASSVRASAKRRRALSGVSGQPSSSRRLWLRYRELTYRNTWINPLLLMGFFYAWFYLSEDQSENHFLHKFLRLSYAVEGTGTGPENPTLYGKGPKDFAFVGFHILFFTFFREFCMQVILHPMAIYCGISKKSKISRFMEQAYSIVYYGLSGPFGLYIMYHTPIWFFNTTAFYEHYPHKTHEALFKLFYLLQASFWAQQSVVLMLQLEKPRKDFYELVFHHIVTVALIFLSYRFHFTWIGLAVYITMDISDVFLATSKTLNYLDSPITKPFFIVFAIVWIYMRHYLNIVFLWSILTEFRTVGDFELNWDTQQYKCWISQIITFGLVFALQLVNLYWLFLIFRIAWRLVFSEIAEDPRSEDDDDDDEDESSPAPESKKDQ"

     misc_feature    4..1152
                     /gene="LAG1"
                     /locus_tag="AWJ20_1430"
                     /note="Protein transporter of the TRAM (translocating
                     chain-associating membrane) superfamily, longevity
                     assurance factor [Intracellular trafficking and
                     secretion]; Region: LAG1; COG5058"
                     /db_xref="CDD:227391"
ORIGIN      

atggcaagacagagaagaacttcaagtgttggaaatataccgcttggcgacaacgcaggtccggccctttctacaatgcaaccagcagcctcgagtgttcgggcttctgccaagcggagaagagccttgagcggagtgtcggggcagccctcgtctagtcgtcggctatggctgagatatcgtgaattgacttatcgcaacacttggatcaatccattgttgttaatgggattcttttatgcgtggttttatttgtcggaagaccagtctgaaaaccactttttacacaagttcttgcgtttatcttatgcagttgaaggtactggtactggtcctgaaaaccctaccttgtacggtaaaggacctaaagatttcgcatttgttggtttccatattctttttttcactttctttcgagaattctgtatgcaagtgattcttcatccaatggccatttactgtggcatcagcaagaaatctaagatctcccggttcatggaacaagcttattccattgtctactacggtctgtccggtcctttcggtttgtacattatgtaccatacacccatctggttcttcaacaccactgccttttacgaacactatcctcataagacccatgaggctctttttaagctgttctatctgttacaagcgtctttctgggcccaacagtcggtggttctaatgctgcaattggaaaagcctcgtaaagatttctacgagcttgtttttcaccatatagtgactgtggctctcatcttcctgtcgtaccgattccatttcacctggatcggtcttgctgtatacattactatggacatcagtgacgtgtttttagccacttcgaagaccctcaactatctggattcccctataaccaagccctttttcattgttttcgccattgtttggatatacatgcgtcactatttaaacattgtgttcctatggtctattctcaccgagttccgtaccgttggcgactttgaactaaactgggacacccagcaatacaagtgctggatctcacaaatcattacctttggtcttgtatttgccctacagttggtcaacctgtactggctgttcctgattttccgcatcgcctggagattggtgttctctgaaatcgccgaggacccccggtccgaagacgacgacgatgacgacgaagatgaatcctcgcccgctcccgagtccaagaaagaccaatag

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