LOCUS       NM_001412181            2757 bp    mRNA    linear   PRI 10-APR-2024
DEFINITION  Homo sapiens tRNA-yW synthesizing protein 1 homolog B (TYW1B),
            transcript variant 4, mRNA.
ACCESSION   NM_001412181
VERSION     NM_001412181.1
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2757)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   2  (bases 1 to 2757)
  AUTHORS   Willer,C.J., Schmidt,E.M., Sengupta,S., Peloso,G.M., Gustafsson,S.,
            Kanoni,S., Ganna,A., Chen,J., Buchkovich,M.L., Mora,S.,
            Beckmann,J.S., Bragg-Gresham,J.L., Chang,H.Y., Demirkan,A., Den
            Hertog,H.M., Do,R., Donnelly,L.A., Ehret,G.B., Esko,T.,
            Feitosa,M.F., Ferreira,T., Fischer,K., Fontanillas,P., Fraser,R.M.,
            Freitag,D.F., Gurdasani,D., Heikkila,K., Hypponen,E., Isaacs,A.,
            Jackson,A.U., Johansson,A., Johnson,T., Kaakinen,M., Kettunen,J.,
            Kleber,M.E., Li,X., Luan,J., Lyytikainen,L.P., Magnusson,P.K.E.,
            Mangino,M., Mihailov,E., Montasser,M.E., Muller-Nurasyid,M.,
            Nolte,I.M., O'Connell,J.R., Palmer,C.D., Perola,M., Petersen,A.K.,
            Sanna,S., Saxena,R., Service,S.K., Shah,S., Shungin,D., Sidore,C.,
            Song,C., Strawbridge,R.J., Surakka,I., Tanaka,T., Teslovich,T.M.,
            Thorleifsson,G., Van den Herik,E.G., Voight,B.F., Volcik,K.A.,
            Waite,L.L., Wong,A., Wu,Y., Zhang,W., Absher,D., Asiki,G.,
            Barroso,I., Been,L.F., Bolton,J.L., Bonnycastle,L.L., Brambilla,P.,
            Burnett,M.S., Cesana,G., Dimitriou,M., Doney,A.S.F., Doring,A.,
            Elliott,P., Epstein,S.E., Ingi Eyjolfsson,G., Gigante,B.,
            Goodarzi,M.O., Grallert,H., Gravito,M.L., Groves,C.J., Hallmans,G.,
            Hartikainen,A.L., Hayward,C., Hernandez,D., Hicks,A.A., Holm,H.,
            Hung,Y.J., Illig,T., Jones,M.R., Kaleebu,P., Kastelein,J.J.P.,
            Khaw,K.T., Kim,E., Klopp,N., Komulainen,P., Kumari,M.,
            Langenberg,C., Lehtimaki,T., Lin,S.Y., Lindstrom,J., Loos,R.J.F.,
            Mach,F., McArdle,W.L., Meisinger,C., Mitchell,B.D., Muller,G.,
            Nagaraja,R., Narisu,N., Nieminen,T.V.M., Nsubuga,R.N., Olafsson,I.,
            Ong,K.K., Palotie,A., Papamarkou,T., Pomilla,C., Pouta,A.,
            Rader,D.J., Reilly,M.P., Ridker,P.M., Rivadeneira,F., Rudan,I.,
            Ruokonen,A., Samani,N., Scharnagl,H., Seeley,J., Silander,K.,
            Stancakova,A., Stirrups,K., Swift,A.J., Tiret,L.,
            Uitterlinden,A.G., van Pelt,L.J., Vedantam,S., Wainwright,N.,
            Wijmenga,C., Wild,S.H., Willemsen,G., Wilsgaard,T., Wilson,J.F.,
            Young,E.H., Zhao,J.H., Adair,L.S., Arveiler,D., Assimes,T.L.,
            Bandinelli,S., Bennett,F., Bochud,M., Boehm,B.O., Boomsma,D.I.,
            Borecki,I.B., Bornstein,S.R., Bovet,P., Burnier,M., Campbell,H.,
            Chakravarti,A., Chambers,J.C., Chen,Y.I., Collins,F.S.,
            Cooper,R.S., Danesh,J., Dedoussis,G., de Faire,U., Feranil,A.B.,
            Ferrieres,J., Ferrucci,L., Freimer,N.B., Gieger,C., Groop,L.C.,
            Gudnason,V., Gyllensten,U., Hamsten,A., Harris,T.B., Hingorani,A.,
            Hirschhorn,J.N., Hofman,A., Hovingh,G.K., Hsiung,C.A.,
            Humphries,S.E., Hunt,S.C., Hveem,K., Iribarren,C., Jarvelin,M.R.,
            Jula,A., Kahonen,M., Kaprio,J., Kesaniemi,A., Kivimaki,M.,
            Kooner,J.S., Koudstaal,P.J., Krauss,R.M., Kuh,D., Kuusisto,J.,
            Kyvik,K.O., Laakso,M., Lakka,T.A., Lind,L., Lindgren,C.M.,
            Martin,N.G., Marz,W., McCarthy,M.I., McKenzie,C.A., Meneton,P.,
            Metspalu,A., Moilanen,L., Morris,A.D., Munroe,P.B., Njolstad,I.,
            Pedersen,N.L., Power,C., Pramstaller,P.P., Price,J.F., Psaty,B.M.,
            Quertermous,T., Rauramaa,R., Saleheen,D., Salomaa,V.,
            Sanghera,D.K., Saramies,J., Schwarz,P.E.H., Sheu,W.H.,
            Shuldiner,A.R., Siegbahn,A., Spector,T.D., Stefansson,K.,
            Strachan,D.P., Tayo,B.O., Tremoli,E., Tuomilehto,J., Uusitupa,M.,
            van Duijn,C.M., Vollenweider,P., Wallentin,L., Wareham,N.J.,
            Whitfield,J.B., Wolffenbuttel,B.H.R., Ordovas,J.M., Boerwinkle,E.,
            Palmer,C.N.A., Thorsteinsdottir,U., Chasman,D.I., Rotter,J.I.,
            Franks,P.W., Ripatti,S., Cupples,L.A., Sandhu,M.S., Rich,S.S.,
            Boehnke,M., Deloukas,P., Kathiresan,S., Mohlke,K.L., Ingelsson,E.
            and Abecasis,G.R.
  CONSRTM   Global Lipids Genetics Consortium
  TITLE     Discovery and refinement of loci associated with lipid levels
  JOURNAL   Nat Genet 45 (11), 1274-1283 (2013)
   PUBMED   24097068
REFERENCE   3  (bases 1 to 2757)
  AUTHORS   Teslovich,T.M., Musunuru,K., Smith,A.V., Edmondson,A.C.,
            Stylianou,I.M., Koseki,M., Pirruccello,J.P., Ripatti,S.,
            Chasman,D.I., Willer,C.J., Johansen,C.T., Fouchier,S.W., Isaacs,A.,
            Peloso,G.M., Barbalic,M., Ricketts,S.L., Bis,J.C., Aulchenko,Y.S.,
            Thorleifsson,G., Feitosa,M.F., Chambers,J., Orho-Melander,M.,
            Melander,O., Johnson,T., Li,X., Guo,X., Li,M., Shin Cho,Y., Jin
            Go,M., Jin Kim,Y., Lee,J.Y., Park,T., Kim,K., Sim,X., Twee-Hee
            Ong,R., Croteau-Chonka,D.C., Lange,L.A., Smith,J.D., Song,K., Hua
            Zhao,J., Yuan,X., Luan,J., Lamina,C., Ziegler,A., Zhang,W.,
            Zee,R.Y., Wright,A.F., Witteman,J.C., Wilson,J.F., Willemsen,G.,
            Wichmann,H.E., Whitfield,J.B., Waterworth,D.M., Wareham,N.J.,
            Waeber,G., Vollenweider,P., Voight,B.F., Vitart,V.,
            Uitterlinden,A.G., Uda,M., Tuomilehto,J., Thompson,J.R., Tanaka,T.,
            Surakka,I., Stringham,H.M., Spector,T.D., Soranzo,N., Smit,J.H.,
            Sinisalo,J., Silander,K., Sijbrands,E.J., Scuteri,A., Scott,J.,
            Schlessinger,D., Sanna,S., Salomaa,V., Saharinen,J., Sabatti,C.,
            Ruokonen,A., Rudan,I., Rose,L.M., Roberts,R., Rieder,M.,
            Psaty,B.M., Pramstaller,P.P., Pichler,I., Perola,M., Penninx,B.W.,
            Pedersen,N.L., Pattaro,C., Parker,A.N., Pare,G., Oostra,B.A.,
            O'Donnell,C.J., Nieminen,M.S., Nickerson,D.A., Montgomery,G.W.,
            Meitinger,T., McPherson,R., McCarthy,M.I., McArdle,W., Masson,D.,
            Martin,N.G., Marroni,F., Mangino,M., Magnusson,P.K., Lucas,G.,
            Luben,R., Loos,R.J., Lokki,M.L., Lettre,G., Langenberg,C.,
            Launer,L.J., Lakatta,E.G., Laaksonen,R., Kyvik,K.O., Kronenberg,F.,
            Konig,I.R., Khaw,K.T., Kaprio,J., Kaplan,L.M., Johansson,A.,
            Jarvelin,M.R., Janssens,A.C., Ingelsson,E., Igl,W., Kees
            Hovingh,G., Hottenga,J.J., Hofman,A., Hicks,A.A., Hengstenberg,C.,
            Heid,I.M., Hayward,C., Havulinna,A.S., Hastie,N.D., Harris,T.B.,
            Haritunians,T., Hall,A.S., Gyllensten,U., Guiducci,C., Groop,L.C.,
            Gonzalez,E., Gieger,C., Freimer,N.B., Ferrucci,L., Erdmann,J.,
            Elliott,P., Ejebe,K.G., Doring,A., Dominiczak,A.F., Demissie,S.,
            Deloukas,P., de Geus,E.J., de Faire,U., Crawford,G., Collins,F.S.,
            Chen,Y.D., Caulfield,M.J., Campbell,H., Burtt,N.P.,
            Bonnycastle,L.L., Boomsma,D.I., Boekholdt,S.M., Bergman,R.N.,
            Barroso,I., Bandinelli,S., Ballantyne,C.M., Assimes,T.L.,
            Quertermous,T., Altshuler,D., Seielstad,M., Wong,T.Y., Tai,E.S.,
            Feranil,A.B., Kuzawa,C.W., Adair,L.S., Taylor,H.A. Jr.,
            Borecki,I.B., Gabriel,S.B., Wilson,J.G., Holm,H.,
            Thorsteinsdottir,U., Gudnason,V., Krauss,R.M., Mohlke,K.L.,
            Ordovas,J.M., Munroe,P.B., Kooner,J.S., Tall,A.R., Hegele,R.A.,
            Kastelein,J.J., Schadt,E.E., Rotter,J.I., Boerwinkle,E.,
            Strachan,D.P., Mooser,V., Stefansson,K., Reilly,M.P., Samani,N.J.,
            Schunkert,H., Cupples,L.A., Sandhu,M.S., Ridker,P.M., Rader,D.J.,
            van Duijn,C.M., Peltonen,L., Abecasis,G.R., Boehnke,M. and
            Kathiresan,S.
  TITLE     Biological, clinical and population relevance of 95 loci for blood
            lipids
  JOURNAL   Nature 466 (7307), 707-713 (2010)
   PUBMED   20686565
REFERENCE   4  (bases 1 to 2757)
  AUTHORS   Noma,A. and Suzuki,T.
  TITLE     Ribonucleome analysis identified enzyme genes responsible for
            wybutosine synthesis
  JOURNAL   Nucleic Acids Symp Ser (Oxf) (50), 65-66 (2006)
   PUBMED   17150819
REFERENCE   5  (bases 1 to 2757)
  AUTHORS   Waas,W.F., de Crecy-Lagard,V. and Schimmel,P.
  TITLE     Discovery of a gene family critical to wyosine base formation in a
            subset of phenylalanine-specific transfer RNAs
  JOURNAL   J Biol Chem 280 (45), 37616-37622 (2005)
   PUBMED   16162496
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC211469.4.
            
            Summary: Wybutosine is a hypermodified guanosine found in
            phenylalanine tRNA. Wybutosine functions to stabilize
            codon-anticodon interactions during ribosome decoding and therefore
            supports the maintenance of the reading frame. In yeast, the
            homolog of this gene is essential for the synthesis of wybutosine.
            The human genome contains two closely related genes that putatively
            function in wybutosine synthesis. The open reading frame of this
            locus is disrupted in some individuals. Thus, this locus appears to
            be an evolving pseudogene, but may still be functional in some
            members of the population. [provided by RefSeq, Apr 2014].
            
            ##Evidence-Data-START##
            Transcript exon combination :: DB229087.1, DB127909.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2162328, SAMN03568911
                                           [ECO:0000348]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-129               AC211469.4         80539-80667
            130-260             AC211469.4         81754-81884
            261-362             AC211469.4         93258-93359
            363-557             AC211469.4         98074-98268
            558-2757            AC211469.4         101383-103582
FEATURES             Location/Qualifiers
     source          1..2757
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="7"
                     /map="7q11.22-q11.23"
     gene            1..2757
                     /gene="TYW1B"
                     /gene_synonym="LINC00069; NCRNA00069; RSAFD2"
                     /note="tRNA-yW synthesizing protein 1 homolog B"
                     /db_xref="GeneID:441250"
                     /db_xref="HGNC:HGNC:33908"
     exon            1..129
                     /gene="TYW1B"
                     /gene_synonym="LINC00069; NCRNA00069; RSAFD2"
                     /inference="alignment:Splign:2.1.0"
     misc_feature    42..44
                     /gene="TYW1B"
                     /gene_synonym="LINC00069; NCRNA00069; RSAFD2"
                     /note="upstream in-frame stop codon"
     CDS             126..911
                     /gene="TYW1B"
                     /gene_synonym="LINC00069; NCRNA00069; RSAFD2"
                     /EC_number="4.1.3.44"
                     /note="isoform 4 is encoded by transcript variant 4;
                     radical S-adenosyl methionine and flavodoxin domains 1;
                     tRNA-yW synthesizing protein 1 homolog B (non-protein
                     coding); tRNA wybutosine-synthesizing protein 1 homolog B;
                     radical S-adenosyl methionine and flavodoxin
                     domain-containing protein 2; long intergenic non-protein
                     coding RNA 69; S-adenosyl-L-methionine-dependent tRNA
                     4-demethylwyosine synthase TYW1B"
                     /codon_start=1
                     /product="S-adenosyl-L-methionine-dependent tRNA
                     4-demethylwyosine synthase TYW1B isoform 4"
                     /protein_id="NP_001399110.1"
                     /db_xref="GeneID:441250"
                     /db_xref="HGNC:HGNC:33908"
                     /translation="

MDPSADTWDLSSPLISLWINRFYIYLGFAVSISLWICVQIVIEMQGFATVLAEAVTSLDLPVAIINLKEYDPDDHLIEEVTSKNVCVFLVATYTDGLPTESAEWFCKWLEEASIDFRFGKTYLKGMRDAVFGLGNSAYASHFNKVGKNVDKWLWMLGVHRVMSRGEGDCDVVKSKHGSIEANFRAWKTKFISQLQALQKGERKKSCGGHCKKGKCESHQHGSEEREEGSQEQDELHHRDTKVYRLCVHLLMLSPSAWRSRA"

     misc_feature    258..>725
                     /gene="TYW1B"
                     /gene_synonym="LINC00069; NCRNA00069; RSAFD2"
                     /note="Flavoprotein (flavin reductase) subunit CysJ of
                     sulfite and N-hydroxylaminopurine reductases [Nucleotide
                     transport and metabolism, Inorganic ion transport and
                     metabolism]; Region: CysJ; COG0369"
                     /db_xref="CDD:440138"
     exon            130..260
                     /gene="TYW1B"
                     /gene_synonym="LINC00069; NCRNA00069; RSAFD2"
                     /inference="alignment:Splign:2.1.0"
     exon            261..362
                     /gene="TYW1B"
                     /gene_synonym="LINC00069; NCRNA00069; RSAFD2"
                     /inference="alignment:Splign:2.1.0"
     exon            363..557
                     /gene="TYW1B"
                     /gene_synonym="LINC00069; NCRNA00069; RSAFD2"
                     /inference="alignment:Splign:2.1.0"
     exon            558..2757
                     /gene="TYW1B"
                     /gene_synonym="LINC00069; NCRNA00069; RSAFD2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      922..927
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TYW1B"
                     /gene_synonym="LINC00069; NCRNA00069; RSAFD2"
                     /note="hexamer: AATAAA"
     polyA_site      956
                     /gene="TYW1B"
                     /gene_synonym="LINC00069; NCRNA00069; RSAFD2"
                     /note="major polyA site"
     regulatory      2732..2737
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TYW1B"
                     /gene_synonym="LINC00069; NCRNA00069; RSAFD2"
                     /note="hexamer: ATTAAA"
     polyA_site      2757
                     /gene="TYW1B"
                     /gene_synonym="LINC00069; NCRNA00069; RSAFD2"
                     /note="major polyA site"
ORIGIN      

acagttctgcagccactcggtacggcgctaacgccgcgaggtagctcggtgcgtctcgcagtaccagtgcgaacctttgggctctccaggtccgagatcctagtctcctgtcggctctgaggaggatggatccttctgcggatacatgggacctctcctcacctttaatatcattatggataaacaggttttacatttatctgggctttgctgttagcattagcctttggatttgtgtccagattgtcatcgagatgcagggatttgcaacagttcttgctgaagcagttacgtccctggatctgcctgtggccattattaatctaaaagaatatgatccagatgatcatctgatagaagaggtgactagtaaaaatgtctgtgtcttcctggttgcgacatacactgacggcctaccaaccgaaagtgcagagtggttctgcaaatggttagaggaagcatccattgattttcgatttggcaaaacttacctgaagggtatgagagatgcggtatttggcctgggaaattctgcctatgctagccacttcaacaaggttggcaaaaatgttgacaagtggctctggatgcttggcgtgcatcgtgtgatgagtcgaggggagggcgactgcgacgtggttaaaagcaagcacggcagcattgaggccaacttcagagcatggaagaccaagttcatctcccagctgcaggcacttcagaaaggggagagaaagaagtcctgtggcggccactgcaagaaaggcaaatgtgaatctcaccaacatggctcagaggagagggaggaaggatctcaagagcaggacgaattgcatcacagagacaccaaggtataccgcctgtgtgttcatctcttgatgctttccccctctgcttggagatctcgagcttgagctcttcctcaataaaccatctcgttggcttctggttagaaggaagatctggctgagtgcagtggctcgcacttgtaatccccacactttgggaggccaaggtgggggtattgcttgagtccaggagttcaagaccagcctgggcaacatagcaataccctatctttaccacaataattttaaatttagcaaggcatggtggtgcatgcctgttgtctcagctacgtaggaggtggagttgggaggattgcttgagcccaggaatttgagactgcagtgagctgtgatcatgccactgccctccagcctgggcagcagagtgagaccctgccccaaaaacaaagacagaaaaaataacaaaaaaagaaggaagatgtgaaactcatgtggtggtgtttcagagagagagagaggccttaggaattaagccatatcgtgctagatgctttccagaatctcttctttaggccgggcacggtggctcacgcttgtaatcccagcacttttgggaggccaaggtgggtggatcacttgaagtcaagagttcgagaccatgctggccaacatggcgaaaccccacctctactaaaaatacaaagattagccaggtgtggtggcgggcacctgtaatcccagctactcgggaggctgaggcatgagaatcgcttgaacccgggaggtggaggtcacagtgatctgagatcacgcgactgcactccagcctgggtgacagagtgagactcagtctcaaaaaaaaaaaaaaaacccacacacacacaaccaaaacgtcttctttaatccttgcattgttagagtcaagtagatttttaccacattttgcaagttgcgaaacggagccacagagaagttaaatttgtcccagcctgagctcacgtctcattatcctgagatcacacatacctcctgacttcaatgactccattagtcactcaaactgaaggctgcaagtcatcctagattccgctttttccctcacattctgagcatttctcttgcttccttctttaaataccccaattcctttgaagctcctgtcttcaggctgtaacactcctgtcatcttcacagctgttatcttttggccacctggtcagttgcataattcagcagcaactttacattctgactcattgtttctctctccatccctgttcctatcattattctttggggctgtggcatccttgcggatgatctgtctagcaccctggcctgcagtttcttggccttcacacctccagcaaccatttctccactgaccttggtctctcccttacataggcacatctgaaattcaagttcctctcatgcttactatgacctcattctgtggcctctactttaacaacactccttttttttttttttgagaaagggtctcattctgtcacccccagtcagagtacaacggtatgatcatggctcactgcagcctcaacctccccaggcagtggtgatcctcccacttcagcctcccaagtagctgggattataggcatgtgccaccatgcctagctaatttgtctattttttgcagagatggagtttcaccatgttgcccaggctggttttgaacacttgggctcaagtgatccactggcccggcctcccaaaatgctgggatcataggcatgagcaactgtgcctggcctttaaaattcttttgatctggccgggcgtggtggctcacgcctgtaatcccagcactttgggaggctgaggcgggtagatcattcaggcagccacccgtggatttggagcccactgcatggctccttttcgtattcctaaaccctttcattaaagcattgccccttttgatgta

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