LOCUS       NM_001178322            1377 bp    mRNA    linear   PLN 17-DEC-2024
DEFINITION  Saccharomyces cerevisiae S288C
            dolichyl-P-Man:Man(5)GlcNAc(2)-PP-dolichol
            alpha-1,3-mannosyltransferase (ALG3), partial mRNA.
ACCESSION   NM_001178322
VERSION     NM_001178322.1
DBLINK      BioProject: PRJNA128
KEYWORDS    RefSeq.
SOURCE      Saccharomyces cerevisiae S288C
  ORGANISM  Saccharomyces cerevisiae S288C
            Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina;
            Saccharomycetes; Saccharomycetales; Saccharomycetaceae;
            Saccharomyces.
REFERENCE   1  (bases 1 to 1377)
  AUTHORS   Engel,S.R., Wong,E.D., Nash,R.S., Aleksander,S., Alexander,M.,
            Douglass,E., Karra,K., Miyasato,S.R., Simison,M., Skrzypek,M.S.,
            Weng,S. and Cherry,J.M.
  TITLE     New data and collaborations at the Saccharomyces Genome Database:
            updated reference genome, alleles, and the Alliance of Genome
            Resources
  JOURNAL   Genetics 220 (4) (2022)
   PUBMED   34897464
REFERENCE   2  (bases 1 to 1377)
  AUTHORS   Goffeau,A., Barrell,B.G., Bussey,H., Davis,R.W., Dujon,B.,
            Feldmann,H., Galibert,F., Hoheisel,J.D., Jacq,C., Johnston,M.,
            Louis,E.J., Mewes,H.W., Murakami,Y., Philippsen,P., Tettelin,H. and
            Oliver,S.G.
  TITLE     Life with 6000 genes
  JOURNAL   Science 274 (5287), 546 (1996)
   PUBMED   8849441
REFERENCE   3  (bases 1 to 1377)
  AUTHORS   Feldmann,H., Aigle,M., Aljinovic,G., Andre,B., Baclet,M.C.,
            Barthe,C., Baur,A., Becam,A.M., Biteau,N., Boles,E. et al.
  TITLE     Complete DNA sequence of yeast chromosome II
  JOURNAL   EMBO J. 13 (24), 5795-5809 (1994)
   PUBMED   7813418
REFERENCE   4  (bases 1 to 1377)
  CONSRTM   NCBI Genome Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-DEC-2024) National Center for Biotechnology
            Information, NIH, Bethesda, MD 20894, USA
REFERENCE   5  (bases 1 to 1377)
  CONSRTM   Saccharomyces Genome Database
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2012) Department of Genetics, Stanford
            University, Stanford, CA 94305-5120, USA
  REMARK    Protein update by submitter
REFERENCE   6  (bases 1 to 1377)
  CONSRTM   Saccharomyces Genome Database
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2011) Department of Genetics, Stanford
            University, Stanford, CA 94305-5120, USA
  REMARK    Sequence update by submitter
REFERENCE   7  (bases 1 to 1377)
  CONSRTM   Saccharomyces Genome Database
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2009) Department of Genetics, Stanford
            University, Stanford, CA 94305-5120, USA
COMMENT     REVIEWED REFSEQ: This record has been curated by SGD. This record
            is derived from an annotated genomic sequence (NC_001134).
            
            ##Genome-Annotation-Data-START##
            Annotation Provider :: SGD
            Annotation Status   :: Full Annotation
            Annotation Version  :: R64-4-1
            URL                 :: http://www.yeastgenome.org/
            ##Genome-Annotation-Data-END##
            COMPLETENESS: incomplete on both ends.
FEATURES             Location/Qualifiers
     source          1..1377
                     /organism="Saccharomyces cerevisiae S288C"
                     /mol_type="mRNA"
                     /strain="S288C"
                     /db_xref="taxon:559292"
                     /chromosome="II"
     gene            <1..>1377
                     /gene="ALG3"
                     /locus_tag="YBL082C"
                     /gene_synonym="RHK1"
                     /db_xref="GeneID:852196"
     CDS             1..1377
                     /gene="ALG3"
                     /locus_tag="YBL082C"
                     /gene_synonym="RHK1"
                     /EC_number="2.4.1.258"
                     /experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
                     reticulum [PMID:26928762]"
                     /experiment="EXISTENCE:direct assay:GO:0006488
                     dolichol-linked oligosaccharide biosynthetic process
                     [PMID:11308030]"
                     /experiment="EXISTENCE:direct assay:GO:0052925
                     dol-P-Man:Man(5)GlcNAc(2)-PP-Dol
                     alpha-1,3-mannosyltransferase activity [PMID:11308030]"
                     /experiment="EXISTENCE:mutant phenotype:GO:0005783
                     endoplasmic reticulum [PMID:8842708]"
                     /experiment="EXISTENCE:mutant phenotype:GO:0006486 protein
                     glycosylation [PMID:8842708]"
                     /experiment="EXISTENCE:mutant phenotype:GO:0006488
                     dolichol-linked oligosaccharide biosynthetic process
                     [PMID:8842708]"
                     /note="Dolichol-P-Man dependent alpha(1-3)
                     mannosyltransferase; involved in synthesis of
                     dolichol-linked oligosaccharide donor for N-linked
                     glycosylation of proteins; G353A missense mutation in
                     human ortholog ALG3 implicated in carbohydrate deficient
                     glycoprotein syndrome type IV, which is characterized by
                     microcephaly, severe epilepsy, minimal psychomotor
                     development, partial deficiency of sialic acids in serum
                     glycoproteins; wild-type human ALG3 can complement yeast
                     alg3 mutant"
                     /codon_start=1
                     /product="dolichyl-P-Man:Man(5)GlcNAc(2)-PP-dolichol
                     alpha-1,3-mannosyltransferase"
                     /protein_id="NP_009471.1"
                     /db_xref="GeneID:852196"
                     /db_xref="SGD:S000000178"
                     /translation="

MEGEQSPQGEKSLQRKQFVRPPLDLWQDLKDGVRYVIFDCRANLIVMPLLILFESMLCKIIIKKVAYTEIDYKAYMEQIEMIQLDGMLDYSQVSGGTGPLVYPAGHVLIYKMMYWLTEGMDHVERGQVFFRYLYLLTLALQMACYYLLHLPPWCVVLACLSKRLHSIYVLRLFNDCFTTLFMVVTVLGAIVASRCHQRPKLKKSLALVISATYSMAVSIKMNALLYFPAMMISLFILNDANVILTLLDLVAMIAWQVAVAVPFLRSFPQQYLHCAFNFGRKFMYQWSINWQMMDEEAFNDKRFHLALLISHLIALTTLFVTRYPRILPDLWSSLCHPLRKNAVLNANPAKTIPFVLIASNFIGVLFSRSLHYQFLSWYHWTLPILIFWSGMPFFVGPIWYVLHEWCWNSYPPNSQASTLLLALNTVLLLLLALTQLSGSVALAKSHLRTTSSMEKKLN"

     misc_feature    142..1251
                     /gene="ALG3"
                     /locus_tag="YBL082C"
                     /gene_synonym="RHK1"
                     /note="ALG3 protein; Region: ALG3; pfam05208"
                     /db_xref="CDD:461587"
ORIGIN      

atggaaggtgaacagtctccgcaaggtgaaaagtctctgcaaaggaagcaatttgtcagacctccgctggatctgtggcaggatctcaaggacggtgtgcgctacgtgatcttcgattgtagggccaatcttatcgttatgccccttttgattttgttcgaaagcatgctgtgcaagattatcattaagaaggtagcttacacagagatcgattacaaggcgtacatggagcagatcgagatgattcagctcgatggcatgctggactactctcaggtgagtggtggaacgggcccgctggtgtatccagcaggccacgtcttgatctacaagatgatgtactggctaacagagggaatggaccacgttgagcgcgggcaagtgtttttcagatacttgtatctccttacactggcgttacaaatggcgtgttactaccttttacatctaccaccgtggtgtgtggtcttggcgtgcctctctaaaagattgcactctatttacgtgctacggttattcaatgattgcttcactactttgtttatggtcgtcacggttttgggggctatcgtggccagcaggtgccatcagcgccccaaattaaagaagtcccttgcgctggtgatctccgcaacatacagtatggctgtgagcattaagatgaatgcgctgttgtatttccctgcaatgatgatttctctattcatccttaatgacgcgaacgtaatccttactttgttggatctcgttgcgatgattgcatggcaagtcgcagttgcagtgcccttcctgcgcagctttccgcaacagtacctgcattgcgcttttaatttcggcaggaagtttatgtaccaatggagtatcaattggcaaatgatggatgaagaggctttcaatgataagaggttccacttggcccttttaatcagccacctgatagcgctcaccacactgttcgtcacaagataccctcgcatcctgcccgatttatggtcttccctgtgccatccgctgaggaaaaatgcagtgctcaatgccaatcccgccaagactattccattcgttctaatcgcatccaacttcatcggcgtcctattttcaaggtccctccactaccagtttctatcctggtatcactggactttgcctatactgatcttttggtcgggaatgcccttcttcgttggtcccatttggtacgtcttgcacgagtggtgctggaattcctatccaccaaactcacaagcaagcacgctattgttggcattgaatactgttctgttgcttctattggccttgacgcagctatctggttcggtcgccctcgccaaaagccatcttcgtaccaccagctctatggaaaaaaagctcaactga

//