LOCUS       NM_138884               3255 bp    mRNA    linear   ROD 26-JUN-2025
DEFINITION  Rattus norvegicus aldo-keto reductase family 1, member D1 (Akr1d1),
            mRNA.
ACCESSION   NM_138884 XM_346688
VERSION     NM_138884.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 3255)
  AUTHORS   Zhu,Y., Fillenwarth,M.J., Crabb,D., Lumeng,L. and Lin,R.C.
  TITLE     Identification of the 37-kd rat liver protein that forms an
            acetaldehyde adduct in vivo as delta 4-3-ketosteroid 5
            beta-reductase
  JOURNAL   Hepatology 23 (1), 115-122 (1996)
   PUBMED   8550030
REFERENCE   2  (bases 1 to 3255)
  AUTHORS   Kondo,K.H., Kai,M.H., Setoguchi,Y., Eggertsen,G., Sjoblom,P.,
            Setoguchi,T., Okuda,K.I. and Bjorkhem,I.
  TITLE     Cloning and expression of cDNA of human delta 4-3-oxosteroid 5
            beta-reductase and substrate specificity of the expressed enzyme
  JOURNAL   Eur J Biochem 219 (1-2), 357-363 (1994)
   PUBMED   7508385
REFERENCE   3  (bases 1 to 3255)
  AUTHORS   Onishi,Y., Noshiro,M., Shimosato,T. and Okuda,K.
  TITLE     delta 4-3-Oxosteroid 5 beta-reductase. Structure and function
  JOURNAL   Biol Chem Hoppe Seyler 372 (12), 1039-1049 (1991)
   PUBMED   1789929
REFERENCE   4  (bases 1 to 3255)
  AUTHORS   Onishi,Y., Noshiro,M., Shimosato,T. and Okuda,K.
  TITLE     Molecular cloning and sequence analysis of cDNA encoding delta
            4-3-ketosteroid 5 beta-reductase of rat liver
  JOURNAL   FEBS Lett 283 (2), 215-218 (1991)
   PUBMED   1710579
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000004.1.
            
            On Dec 2, 2020 this sequence version replaced NM_138884.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: D17309.1, FQ219730.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760400, SAMEA5760476
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-153               JAXUCZ010000004.1  67121294-67121446
            154-318             JAXUCZ010000004.1  67123764-67123928
            319-435             JAXUCZ010000004.1  67127059-67127175
            436-513             JAXUCZ010000004.1  67136531-67136608
            514-636             JAXUCZ010000004.1  67141161-67141283
            637-746             JAXUCZ010000004.1  67143311-67143420
            747-912             JAXUCZ010000004.1  67144850-67145015
            913-995             JAXUCZ010000004.1  67149990-67150072
            996-3255            JAXUCZ010000004.1  67152284-67154543
FEATURES             Location/Qualifiers
     source          1..3255
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="4"
                     /map="4q23"
     gene            1..3255
                     /gene="Akr1d1"
                     /note="aldo-keto reductase family 1, member D1"
                     /db_xref="GeneID:192242"
                     /db_xref="RGD:620752"
     exon            1..153
                     /gene="Akr1d1"
                     /inference="alignment:Splign:2.1.0"
     CDS             61..1038
                     /gene="Akr1d1"
                     /EC_number="1.3.1.3"
                     /note="3-oxo-5-beta-steroid 4-dehydrogenase;
                     delta(4)-3-oxosteroid 5-beta-reductase;
                     delta(4)-3-ketosteroid 5-beta-reductase; aldo-keto
                     reductase family 1, member D1 (delta
                     4-3-ketosteroid-5-beta-reductase)"
                     /codon_start=1
                     /product="aldo-keto reductase family 1 member D1"
                     /protein_id="NP_620239.2"
                     /db_xref="GeneID:192242"
                     /db_xref="RGD:620752"
                     /translation="

MNLSTANHHIPLNDGNSIPIIGLGTYSDPRPVPGKTFIAVKTAIDEGYRHIDGAYVYRNEHEVGEAIREKVAEGKVKREEIFYCGKLWSTDHDPEMVRPALERTLQTLKLDYIDLYIIEMPMAFKPGEEFYPKDENGRVIYHKSNLCATWEALEACKDAGLVKSLGVSNFNRRQLEVILNKPGLKYKPVTNQVECHPYFTQTKLLKFCQQHDIVIVAYSPLGTCRNPLWVNVSSPPLLKDELLTSLGKKYNKTQAQIVLRFDIQRGLVVIPKSTTPERIKENFQIFDFSLTKEEMKDIEALNKNVRFVEMLMWSDHPEYPFHDEY"

     misc_feature    103..1023
                     /gene="Akr1d1"
                     /note="AKR1D family of aldo-keto reductase (AKR); Region:
                     AKR_AKR1D1-3; cd19109"
                     /db_xref="CDD:381335"
     misc_feature    order(130..138,214..216,229..231,316..318,322..324,
                     415..417,451..453,562..567,634..636,712..732,745..747,
                     772..774,823..825,868..885,892..894,901..906,997..999)
                     /gene="Akr1d1"
                     /note="active site"
                     /db_xref="CDD:381335"
     misc_feature    order(130..138,214..216,229..231)
                     /gene="Akr1d1"
                     /note="catalytic tetrad [active]"
                     /db_xref="CDD:381335"
     exon            154..318
                     /gene="Akr1d1"
                     /inference="alignment:Splign:2.1.0"
     exon            319..435
                     /gene="Akr1d1"
                     /inference="alignment:Splign:2.1.0"
     exon            436..513
                     /gene="Akr1d1"
                     /inference="alignment:Splign:2.1.0"
     exon            514..636
                     /gene="Akr1d1"
                     /inference="alignment:Splign:2.1.0"
     exon            637..746
                     /gene="Akr1d1"
                     /inference="alignment:Splign:2.1.0"
     exon            747..912
                     /gene="Akr1d1"
                     /inference="alignment:Splign:2.1.0"
     exon            913..995
                     /gene="Akr1d1"
                     /inference="alignment:Splign:2.1.0"
     exon            996..3255
                     /gene="Akr1d1"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      

ctatttgtacttctgtcctgttcagaatcactacttgaagcttcagatcccttctctacaatgaacctcagcactgcaaaccaccacatacccctaaatgatggtaacagcattccgatcatcgggcttggaacctactcagaccctagaccggtacccggcaagacctttatagcagtgaagacagctattgacgaggggtaccggcatattgatggggcctacgtctaccgaaatgaacatgaagttggtgaggccatcagagaaaaggtggcagaagggaaggtaaagagggaagagattttctactgtggaaagttatggagtacagaccatgatccagagatggtccgcccagccctggaaaggaccctgcagaccctcaagctagattacatagacctttatatcattgagatgcccatggcctttaagcctggagaggaattttatcctaaagatgagaatggccgagtgatataccacaaatcaaatctgtgtgccacgtgggaggcactggaagcttgcaaagacgctggcttggtgaaatcccttggggtgtctaattttaaccgcaggcagctggaggtcatcttgaacaagccaggactcaagtacaagcctgtcaccaaccaggtggagtgccacccgtatttcacccagacaaaactcttgaagttctgccagcagcatgacatcgtcattgtcgcatacagccccttagggacctgtcgcaacccgttatgggtgaatgtatcttctccacccttgttaaaggatgaactcctaacctcgctggggaaaaagtacaataagacacaagctcaaattgtgttgcgtttcgacatccagcgagggttggttgtcatccctaaaagtactacccctgaaaggatcaaagaaaactttcagatctttgacttctctctcaccaaagaagaaatgaaggacattgaagccttgaataaaaacgtgcgctttgtggagatgctcatgtggagtgatcatcctgaatacccatttcatgacgaatactgaacatggaaatttcttcagtggagttttttttgtctttcattcgggatcttgagctgattaatcccctccagtcgtagaaataaatggggtcttgctatcttcagattaagttgagcagaatacaccatgcttaacttgtgtatagtattaattcaacttagctctgatgaagagaagatttaaatatatctaaatagtttgttggaaaatgctttactagtatctttagctcaatgtgttctagattgttgatggaaattaccgaactttaattcaaaaataacaaaatatataaaatacaagatacaatataaaatataaaatacaaaaatataaaaacaggttctaacccaggagtctgagactaccatagggagtttgatttgtttagcatttagcaggacaacaaagaggttggcatgcttccaggtgctgaccataaccatgacccaaacaaggtcatggacacaagatggacacagccaagacacaaatccacataccaatattaatgcaccttgagtatagacagaactggaagtctagaaagtcctaaagaagaacataaagcaggatgtcccggggctagaggttctcactggaggacttgcctggcagactggagacccagggtgagggtgtgggtgaggactatcagcaaagccataacgattctgtttttaccctttgtttccatccagtgtgcccattcagcttttaatgtgcttgaaggacagacagttaccgattccttcttaaattgtttttgttcagggcagtggggaattgagacagggtctcaggtagtctgctcatgtagctgcttacaaacttgctgtgcagctgagaccagttttgaattctgaacccctggtctctcttccatttttcatggcctgagcttccacctgactggtaagccccacttgtactgtgctgtaatcattcttgcccacattcatcaaggaaaccaccatgtaaagtggcaagaaacaatcaatatatacaaaccaagatttgtatcataaaatggtctgaagatggtgatttttaaaagaatgatattttaaaatagcccatttctttctttctttctgaatcttttaaaaattatttttattttatgtgcattggtgtttagcctgcatgtgtatctatgagaattgtttgaactcatggaactggagttacagacagtggtgagctgtaggtaggtgctgggaattgaacccaagtcattgggaagagctgcaagtgctcttaactgctgagccatctccccagcccccaaatgaatttgggaaatactagaagataaagctttgtaaaagtagttttttaagatcttaagaatatattgaggtagctatctgaattttgacaaagctgcaagttcttttttttaaattggatttttaaaaatttatttacatttcaaatgttatcccctctcctgggttcccatccacagacctccctatctcatcccccctctcccttcttctatgagagtgttcacccacctacccactccttccctcctccctgccctgacattcccctacactagggggtcgagtcttggcaggaccaagggcctctcctcccattggtgcccaacaaggccatcctctgctacataggcagctggagccatgggtctgtccatgtgtactctctggatggtggtttagtccctgggagctctggttggttggttctgttgttatggggttgcaaaccccttcagctcctttaatcctttctctaattcctccattggggaccctgttctcagttctatggttggctgtgagcatccacctgtgtatttgtcatgctctggcagagcctctcaggagaatcaggctcctgtcagcagagcacaagtaattttagcgtatataaatttttaaaaagtgaattaaatgattacaactgtatctatttttaattcatctgaaatacagtacatttgtttatattagatcacttccaaagggtgagagaattcttcaaaaataatatatttttaatatcaatagaagaaatcaaatggcccaaagagaaacaagtttttgagaaattatcacttaaaaaatgtgatcaacatgaaccatttttgttcaatactgttcaatattgttaacaatgctgttctctggaaaatattttattcacatgaattaataaaatataatgatttgttctaccaca

//