LOCUS       NM_031737               3221 bp    mRNA    linear   ROD 20-MAR-2023
DEFINITION  Rattus norvegicus NK6 homeobox 1 (Nkx6-1), mRNA.
ACCESSION   NM_031737 XM_346455
VERSION     NM_031737.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 3221)
  AUTHORS   Pavluch V, Engstova H, Spackova J and Jezek P.
  TITLE     Deficiency of transcription factor Nkx6.1 does not prevent insulin
            secretion in INS-1E cells
  JOURNAL   Sci Rep 13 (1), 683 (2023)
   PUBMED   36639413
  REMARK    GeneRIF: Deficiency of transcription factor Nkx6.1 does not prevent
            insulin secretion in INS-1E cells.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3221)
  AUTHORS   Spacek T, Pavluch V, Alan L, Capkova N, Engstova H, Dlaskova A,
            Berkova Z, Saudek F and Jezek P.
  TITLE     Nkx6.1 decline accompanies mitochondrial DNA reduction but subtle
            nucleoid size decrease in pancreatic islet beta-cells of diabetic
            Goto Kakizaki rats
  JOURNAL   Sci Rep 7 (1), 15674 (2017)
   PUBMED   29142323
  REMARK    GeneRIF: The beta-cell maintenance factor Nkx6.1 mRNA and protein
            were declining with age (>12-fold, 10 months) and decreasing with
            fasting hyperglycemia in Goto Kakizaki rats, probably
            predetermining the impaired mtDNA replication (copy number
            decrease), while spatial expansion of mtDNA kept nucleoids with
            only smaller sizes than those containing much higher mtDNA in
            non-diabetic beta-cells.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3221)
  AUTHORS   Ray JD, Kener KB, Bitner BF, Wright BJ, Ballard MS, Barrett EJ,
            Hill JT, Moss LG and Tessem JS.
  TITLE     Nkx6.1-mediated insulin secretion and beta-cell proliferation is
            dependent on upregulation of c-Fos
  JOURNAL   FEBS Lett 590 (12), 1791-1803 (2016)
   PUBMED   27164028
  REMARK    GeneRIF: These data demonstrate that c-Fos is critical for
            Nkx6.1-mediated expansion of functional beta-cell mass.
REFERENCE   4  (bases 1 to 3221)
  AUTHORS   Hobson A, Draney C, Stratford A, Becker TC, Lu D, Arlotto M and
            Tessem JS.
  TITLE     Aurora Kinase A is critical for the Nkx6.1 mediated beta-cell
            proliferation pathway
  JOURNAL   Islets 7 (1), e1027854 (2015)
   PUBMED   26030060
  REMARK    GeneRIF: The homeobox beta-cell transcription factor Nkx6.1 induces
            beta-cell proliferation by activating the orphan nuclear receptors
            Nr4a1 and Nr4a3.
REFERENCE   5  (bases 1 to 3221)
  AUTHORS   Zhao X, Chen Y, Zhu Q, Huang H, Teng P, Zheng K, Hu X, Xie B, Zhang
            Z, Sander M and Qiu M.
  TITLE     Control of astrocyte progenitor specification, migration and
            maturation by Nkx6.1 homeodomain transcription factor
  JOURNAL   PLoS One 9 (10), e109171 (2014)
   PUBMED   25285789
  REMARK    GeneRIF: Nkx6.1 plays a vital role in astrocyte specification and
            differentiation in the ventral spinal cord.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 3221)
  AUTHORS   Sun T, Dong H, Wu L, Kane M, Rowitch DH and Stiles CD.
  TITLE     Cross-repressive interaction of the Olig2 and Nkx2.2 transcription
            factors in developing neural tube associated with formation of a
            specific physical complex
  JOURNAL   J Neurosci 23 (29), 9547-9556 (2003)
   PUBMED   14573534
REFERENCE   7  (bases 1 to 3221)
  AUTHORS   Vallstedt A, Muhr J, Pattyn A, Pierani A, Mendelsohn M, Sander M,
            Jessell TM and Ericson J.
  TITLE     Different levels of repressor activity assign redundant and
            specific roles to Nkx6 genes in motor neuron and interneuron
            specification
  JOURNAL   Neuron 31 (5), 743-755 (2001)
   PUBMED   11567614
REFERENCE   8  (bases 1 to 3221)
  AUTHORS   Briscoe J, Pierani A, Jessell TM and Ericson J.
  TITLE     A homeodomain protein code specifies progenitor cell identity and
            neuronal fate in the ventral neural tube
  JOURNAL   Cell 101 (4), 435-445 (2000)
   PUBMED   10830170
REFERENCE   9  (bases 1 to 3221)
  AUTHORS   Jorgensen MC, Vestergard Petersen H, Ericson J, Madsen OD and Serup
            P.
  TITLE     Cloning and DNA-binding properties of the rat pancreatic
            beta-cell-specific factor Nkx6.1
  JOURNAL   FEBS Lett 461 (3), 287-294 (1999)
   PUBMED   10567713
REFERENCE   10 (bases 1 to 3221)
  AUTHORS   Oster A, Jensen J, Serup P, Galante P, Madsen OD and Larsson LI.
  TITLE     Rat endocrine pancreatic development in relation to two homeobox
            gene products (Pdx-1 and Nkx 6.1)
  JOURNAL   J Histochem Cytochem 46 (6), 707-715 (1998)
   PUBMED   9603781
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from
            JACYVU010000248.1.
            
            On Nov 26, 2020 this sequence version replaced NM_031737.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF004431.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383, SAMEA5760389
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1470              JACYVU010000248.1  3431892-3433361
            1471-1643           JACYVU010000248.1  3434926-3435098
            1644-3221           JACYVU010000248.1  3437248-3438825
FEATURES             Location/Qualifiers
     source          1..3221
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="14"
                     /map="14p22"
     gene            1..3221
                     /gene="Nkx6-1"
                     /gene_synonym="Nkx6.1; Nkx61; Nkx6a"
                     /note="NK6 homeobox 1"
                     /db_xref="GeneID:65193"
                     /db_xref="RGD:69318"
     exon            1..1470
                     /gene="Nkx6-1"
                     /gene_synonym="Nkx6.1; Nkx61; Nkx6a"
                     /inference="alignment:Splign:2.1.0"
     misc_feature    504..506
                     /gene="Nkx6-1"
                     /gene_synonym="Nkx6.1; Nkx61; Nkx6a"
                     /note="upstream in-frame stop codon"
     CDS             798..1895
                     /gene="Nkx6-1"
                     /gene_synonym="Nkx6.1; Nkx61; Nkx6a"
                     /note="homeobox protein NK-6 homolog A; NK6 transcription
                     factor homolog A; NK6 transcription factor related, locus
                     1; NK homeobox, family 6, A"
                     /codon_start=1
                     /product="homeobox protein Nkx-6.1"
                     /protein_id="NP_113925.1"
                     /db_xref="GeneID:65193"
                     /db_xref="RGD:69318"
                     /translation="

MLAVGAMEGPRQSAFLLSSPPLAALHSMAEMKTPLYPAAYPPLPTGPPSSSSSSSSSSSPSPPLGAHNPGGLKPPAAGGLSSLGSPPQQLSAATPHGINDILSRPSMPVASGAALPSASPSGSSSSSSSSASATSASAAAAAAAAAAAAAASSPAGLLAGLPRFSSLSPPPPPPGLYFSPSAAAVAAVGRYPKPLAELPGRTPIFWPGVMQSPPWRDARLACTPHQGSILLDKDGKRKHTRPTFSGQQIFALEKTFEQTKYLAGPERARLAYSLGMTESQVKVWFQNRRTKWRKKHAAEMATAKKKQDSETERLKGTSENEEDDDDYNKPLDPNSDDEKITQLLKKHKSSGGSLLLHASEAEGSS"

     misc_feature    900..1205
                     /gene="Nkx6-1"
                     /gene_synonym="Nkx6.1; Nkx61; Nkx6a"
                     /note="propagated from UniProtKB/Swiss-Prot (O35762.1);
                     Region: Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite"
     misc_feature    1101..1604
                     /gene="Nkx6-1"
                     /gene_synonym="Nkx6.1; Nkx61; Nkx6a"
                     /note="propagated from UniProtKB/Swiss-Prot (O35762.1);
                     Region: Repressor domain. /evidence=ECO:0000250"
     misc_feature    1365..1367
                     /gene="Nkx6-1"
                     /gene_synonym="Nkx6.1; Nkx61; Nkx6a"
                     /note="Asymmetric dimethylarginine.
                     /evidence=ECO:0000250|UniProtKB:P78426; propagated from
                     UniProtKB/Swiss-Prot (O35762.1); methylation site"
     misc_feature    order(1509..1523,1527..1529,1578..1580,1596..1598,
                     1635..1637,1641..1646,1653..1658,1662..1670,1674..1679)
                     /gene="Nkx6-1"
                     /gene_synonym="Nkx6.1; Nkx61; Nkx6a"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:238039"
     misc_feature    1515..1676
                     /gene="Nkx6-1"
                     /gene_synonym="Nkx6.1; Nkx61; Nkx6a"
                     /note="Homeobox domain; Region: Homeobox; pfam00046"
                     /db_xref="CDD:425441"
     misc_feature    order(1515..1517,1524..1526,1644..1646,1653..1658,
                     1665..1667)
                     /gene="Nkx6-1"
                     /gene_synonym="Nkx6.1; Nkx61; Nkx6a"
                     /note="specific DNA base contacts [nucleotide binding];
                     other site"
                     /db_xref="CDD:238039"
     misc_feature    1680..1892
                     /gene="Nkx6-1"
                     /gene_synonym="Nkx6.1; Nkx61; Nkx6a"
                     /note="propagated from UniProtKB/Swiss-Prot (O35762.1);
                     Region: Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite"
     misc_feature    1716..1892
                     /gene="Nkx6-1"
                     /gene_synonym="Nkx6.1; Nkx61; Nkx6a"
                     /note="propagated from UniProtKB/Swiss-Prot (O35762.1);
                     Region: Involved in DNA-binding. /evidence=ECO:0000250"
     exon            1471..1643
                     /gene="Nkx6-1"
                     /gene_synonym="Nkx6.1; Nkx61; Nkx6a"
                     /inference="alignment:Splign:2.1.0"
     exon            1644..3221
                     /gene="Nkx6-1"
                     /gene_synonym="Nkx6.1; Nkx61; Nkx6a"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      

ccggcttgcacaagcggacggacgatcggaaccggcccgtcagacttttcccacttaggtttattttctctttcttcctttttcggtcccgctactccggcgccgcgtggcccggaggggaagcaggcgcgtgggcgcgccaagtgcccagccgccggagcggtgccgccactgccctttctggcgcagtaacgccggtcccacggcggtagcgggcgcgatctccgcgagagccgtttgcgctccatcccctgggcgagcggcgagtgcgactcagctgcgcggccgccgggagcgcggagcgtccgagcgagaacagaggcgcgcaccgggcggaacgcggcccgctttgaagctcccctaggcgggcgagccggcccccaccctcttacatcaaagcgatcgctccgcgcctcccaaccttgttgcaaactctctgggccagccccgggtacgtcttcctgatttcccgcgggggtggagagatgagaagcagagctctctgagtcgggagcgagggaccagcgcccgggatcgaatcccggactcccggagtcgagaaagcgccaagcgggcccgcgcccctgccgccctcgcccgccgccgcctcccgcggggcatttggacattttctgctgcgcagctcgcaccccagctctcggagcccgccgcagagtcacacttcgcttgcgcgcgagcgcttccccagcgcctccggctctcctgcgcctcgggctctcctgagccccggcggggccaccggcctgcttggctgcgggttcgggtctggctgtgggatgttagctgtgggggcgatggagggcccccggcagagcgcgttcctgctcagcagcccgcccttggccgccctgcacagcatggccgagatgaagaccccgctctaccctgccgcctatcccccgctgcccaccgggcccccctcctcctcgtcctcgtcctcctcgtcctcgtcgccctccccacctttgggcgcacacaacccgggcggtttgaagcccccggccgcggggggcctctcgtccctgggcagccccccgcagcagctttcggcggccactccgcacggcatcaacgacatcctgagccggccctccatgccggtggcctcgggggccgccctgccctccgcctcgccctcggggtcttcttcctcctcctcctcgtccgcctccgccacctcggcctctgcggccgccgctgctgctgccgctgccgccgccgctgccgcctcatcgcccgccgggctgttggccggcctgccccgcttcagcagcctgagccctccgccaccgccgcccgggctctactttagccccagcgccgcggccgtggccgccgtgggccggtaccccaagcccctggccgagctgcccggtcggacgcccatcttctggcctggggtgatgcagagcccgccttggagggacgcgcgccttgcctgtaccccccatcaaggatccattttgttggacaaagatgggaagagaaaacacaccagacccacattctccggccagcagatcttcgccctggagaagactttcgaacaaacgaagtacttggcaggaccagagagagcacgcttggcctattctctggggatgacggagagtcaggtcaaggtctggttccagaatcggcggaccaagtggagaaagaagcacgcagccgagatggccacggccaagaagaagcaggactcggagactgagcggctcaaggggacttcggagaacgaggaggacgacgacgattacaacaagcccctggacccgaactctgacgacgagaaaatcactcagctgctgaaaaagcacaaatccagcggaggcagcctcctgctgcacgcgtcggaggccgagggctcgtcctgagcgcggcccgcaccgcggggatcccgaccgcgccccacagccggtcccgcggtccccccccgcaccccggctgctcgcccggcctttactattttctaagatgtacatatctatttttttaacctagaaattttggcggggagggtgggggtcgtcggtaggacggcgcggtgatggcgaggaaaggagcccgccaagtgcactgctcaaaaaaccccgcagccgaacccgcatccccgcggcttcccaggacccttcccacccgcgacgtcaggcgtccccggtccaggctcggtcctttgctttgcgttccctgagcaccgggccgtgtttccgaaaggtcgtcggccaccgcctggtgggaatctcagagccaccgagggcagctgccaccaagggagaaagggacgctacccctccccccaactgctctgccgaccaagtaacctgttgacggctctctatgtaaataaatcgtgagtcacactgactagaagttactttattgtgaatatttaaaatgtaaaaggctttttggaatttggtataaagagatgaactgtactctcgttgatttaaggtattcacaccacttggccgcctttcatattaacttgaaaagcttagaaaaacgacgtcatcgccctactcagacgcctcccgactcactcctctttcggctctgcttctttcctaaccgcttttctccttcaccaattccggcagggccccaggcagccccaggcccccaccccggtccagctccaagacttgcagtctgagatcccgggacccacgagcttctcacaccagcctttgggttcccttccgaatttgcacagtcgtttgttgctatttattagtgattaaaaaaagatattggggagggaaggactacagtaccttgtatttaatttaactcctttctgataaaaatgcgtgttaaaagtagatggtgggaattgttacaagcgctctgggtcagaacataaacgttccatttaattgctgtaattgaattcgaagtgtttttgtatcataagaatactatagactatgtaaattgttttcttttaagctaataacggtgatatatcagcctctttaacacttattaatttatatgaggaattcggtttgtaaagaaaaggcagcccctttgcgagaaccatgaaatgtaatgcactttctgtttcaaaggataaatcgaattaaaaacagcaacaaaaaatagtttgaagacgtctgttgccttaagggaacatgtacaggttacctggcctcatcagagatctcaaataaagtcaagagcacatttaaaaaaaa

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