LOCUS       NM_017250               2102 bp    mRNA    linear   ROD 28-DEC-2023
DEFINITION  Rattus norvegicus 5-hydroxytryptamine receptor 2B (Htr2b), mRNA.
ACCESSION   NM_017250 XM_017596306
VERSION     NM_017250.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Binetti J, Bertran L, Riesco D, Aguilar C, Martinez S, Sabench F,
            Porras JA, Camaron J, Castillo DD, Richart C and Auguet T.
  TITLE     Deregulated Serotonin Pathway in Women with Morbid Obesity and
            NAFLD
  JOURNAL   Life (Basel) 10 (10), 245 (2020)
   PUBMED   33081272
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2102)
  AUTHORS   Borkowski LF, Craig TA, Stricklin OE, Johnson KA and Nichols NL.
  TITLE     5-HT2A/B receptor expression in the phrenic motor nucleus in a rat
            model of ALS (SOD1G93A)
  JOURNAL   Respir Physiol Neurobiol 279, 103471 (2020)
   PUBMED   32504811
  REMARK    GeneRIF: 5-HT2A/B receptor expression in the phrenic motor nucleus
            in a rat model of ALS (SOD1(G93A)).
REFERENCE   3  (bases 1 to 2102)
  AUTHORS   Perim RR, Fields DP and Mitchell GS.
  TITLE     Cross-talk inhibition between 5-HT2B and 5-HT7 receptors in phrenic
            motor facilitation via NADPH oxidase and PKA
  JOURNAL   Am J Physiol Regul Integr Comp Physiol 314 (5), R709-R715 (2018)
   PUBMED   29384698
  REMARK    GeneRIF: Cross-talk inhibition occurs between 5-HT2B and 5-HT7
            receptors in phrenic motor facilitation via NADPH oxidase and PKA
            pathway.
REFERENCE   4  (bases 1 to 2102)
  AUTHORS   Naito K, Moteki H, Kimura M, Natsume H and Ogihara M.
  TITLE     Serotonin 5-HT2B Receptor-Stimulated DNA Synthesis and
            Proliferation Are Mediated by Autocrine Secretion of Transforming
            Growth Factor-alpha in Primary Cultures of Adult Rat Hepatocytes
  JOURNAL   Biol Pharm Bull 39 (4), 570-577 (2016)
   PUBMED   26804134
  REMARK    GeneRIF: 5-HT stimulates DNA synthesis and proliferation in primary
            cultures of adult rat hepatocytes by acting via autocrine secretion
            of TGF-alpha induced by the serotonin 5-HT2B receptor/Gq/PLC/Ca2+
            pathway.
REFERENCE   5  (bases 1 to 2102)
  AUTHORS   Michishita M, Yano K, Kasahara K, Tomita K and Matsuzaki O.
  TITLE     Increased expression of 5-HT(2A) and 5-HT(2B) receptors in detrusor
            muscle after partial bladder outlet obstruction in rats
  JOURNAL   Biomed Res 36 (3), 187-194 (2015)
   PUBMED   26106048
  REMARK    GeneRIF: pBOO induced up-regulation of the 5-HT(2A) and 5-HT(2B)
            receptors in the detrusor muscle and subserosal layers of the
            bladder, and such up-regulation may be related to the enhanced
            bladder contractile response to 5-HT.
REFERENCE   6  (bases 1 to 2102)
  AUTHORS   Bonhaus DW, Bach C, DeSouza A, Salazar FH, Matsuoka BD, Zuppan P,
            Chan HW and Eglen RM.
  TITLE     The pharmacology and distribution of human 5-hydroxytryptamine2B
            (5-HT2B) receptor gene products: comparison with 5-HT2A and 5-HT2C
            receptors
  JOURNAL   Br J Pharmacol 115 (4), 622-628 (1995)
   PUBMED   7582481
REFERENCE   7  (bases 1 to 2102)
  AUTHORS   Borman RA and Burleigh DE.
  TITLE     Functional evidence for a 5-HT2B receptor mediating contraction of
            longitudinal muscle in human small intestine
  JOURNAL   Br J Pharmacol 114 (8), 1525-1527 (1995)
   PUBMED   7599919
REFERENCE   8  (bases 1 to 2102)
  AUTHORS   Choi DS, Birraux G, Launay JM and Maroteaux L.
  TITLE     The human serotonin 5-HT2B receptor: pharmacological link between
            5-HT2 and 5-HT1D receptors
  JOURNAL   FEBS Lett 352 (3), 393-399 (1994)
   PUBMED   7926008
REFERENCE   9  (bases 1 to 2102)
  AUTHORS   Kursar JD, Nelson DL, Wainscott DB, Cohen ML and Baez M.
  TITLE     Molecular cloning, functional expression, and pharmacological
            characterization of a novel serotonin receptor
            (5-hydroxytryptamine2F) from rat stomach fundus
  JOURNAL   Mol Pharmacol 42 (4), 549-557 (1992)
   PUBMED   1331748
REFERENCE   10 (bases 1 to 2102)
  AUTHORS   Foguet M, Hoyer D, Pardo LA, Parekh A, Kluxen FW, Kalkman HO,
            Stuhmer W and Lubbert H.
  TITLE     Cloning and functional characterization of the rat stomach fundus
            serotonin receptor
  JOURNAL   EMBO J 11 (9), 3481-3487 (1992)
   PUBMED   1505525
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JACYVU010000215.1.
            
            On or before Mar 18, 2021 this sequence version replaced
            XM_017596306.2, NM_017250.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            CDS exon combination :: X66842.1 [ECO:0000331]
            RNAseq introns       :: single sample supports all introns
                                    SAMEA5760389, SAMN06621351 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-101               JACYVU010000215.1  19825672-19825772   c
            102-680             JACYVU010000215.1  19824225-19824803   c
            681-881             JACYVU010000215.1  19815174-19815374   c
            882-2102            JACYVU010000215.1  19811767-19812987   c
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="9"
                     /map="9q35"
     gene            1..2102
                     /gene="Htr2b"
                     /gene_synonym="5-HT-2B; 5-HT-2F; 5-HT2B"
                     /note="5-hydroxytryptamine receptor 2B"
                     /db_xref="GeneID:29581"
                     /db_xref="RGD:61801"
     exon            1..101
                     /gene="Htr2b"
                     /gene_synonym="5-HT-2B; 5-HT-2F; 5-HT2B"
                     /inference="alignment:Splign:2.1.0"
     exon            102..680
                     /gene="Htr2b"
                     /gene_synonym="5-HT-2B; 5-HT-2F; 5-HT2B"
                     /inference="alignment:Splign:2.1.0"
     misc_feature    314..316
                     /gene="Htr2b"
                     /gene_synonym="5-HT-2B; 5-HT-2F; 5-HT2B"
                     /note="upstream in-frame stop codon"
     CDS             332..1771
                     /gene="Htr2b"
                     /gene_synonym="5-HT-2B; 5-HT-2F; 5-HT2B"
                     /note="serotonin receptor 2B; stomach fundus serotonin
                     receptor; 5-hydroxytryptamine (serotonin) receptor 2B, G
                     protein-coupled"
                     /codon_start=1
                     /product="5-hydroxytryptamine receptor 2B"
                     /protein_id="NP_058946.2"
                     /db_xref="GeneID:29581"
                     /db_xref="RGD:61801"
                     /translation="

MASSYKMSEQSTISEHILQKTCDHLILTDRSGLKAESAAEEMKQTAENQGNTVHWAALLIFAVIIPTIGGNILVILAVSLEKRLQYATNYFLMSLAVADLLVGLFVMPIALLTIMFEATWPLPLALCPAWLFLDVLFSTASIMHLCAISLDRYIAIKKPIQANQCNSRTTAFVKITVVWLISIGIAIPVPIKGIEADVVNAHNITCELTKDRFGSFMLFGSLAAFFAPLTIMIVTYFLTIHALRKKAYLVRNRPPQRLTRWTVSTVLQREDSSFSSPEKMAMLDGSHKDKILPNSIDETLMRRMSSAGKKPAQTISNEQRASKVLGIVFLFFLLMWCPFFITNVTLALCDSCNQTTLKTLLQIFVWVGYVSSGVNPLIYTLFNKTFREAFGRYITCNYQATKSVKVLRKCSSTLYFGNSMVENSKFFTKHGIRNGINPAMYQSPVRLRSSTIQSSSIILLNTFLTENDGDKVEDQVSYI"

     misc_feature    491..1522
                     /gene="Htr2b"
                     /gene_synonym="5-HT-2B; 5-HT-2F; 5-HT2B"
                     /note="seven-transmembrane G protein-coupled receptor
                     superfamily; Region: 7tm_GPCRs; cl28897"
                     /db_xref="CDD:452889"
     misc_feature    497..571
                     /gene="Htr2b"
                     /gene_synonym="5-HT-2B; 5-HT-2F; 5-HT2B"
                     /note="TM helix 1 [structural motif]; Region: TM helix 1"
                     /db_xref="CDD:410628"
     misc_feature    497..565
                     /gene="Htr2b"
                     /gene_synonym="5-HT-2B; 5-HT-2F; 5-HT2B"
                     /note="propagated from UniProtKB/Swiss-Prot (P30994.1);
                     transmembrane region"
     misc_feature    596..664
                     /gene="Htr2b"
                     /gene_synonym="5-HT-2B; 5-HT-2F; 5-HT2B"
                     /note="TM helix 2 [structural motif]; Region: TM helix 2"
                     /db_xref="CDD:410628"
     misc_feature    599..667
                     /gene="Htr2b"
                     /gene_synonym="5-HT-2B; 5-HT-2F; 5-HT2B"
                     /note="propagated from UniProtKB/Swiss-Prot (P30994.1);
                     transmembrane region"
     misc_feature    713..781
                     /gene="Htr2b"
                     /gene_synonym="5-HT-2B; 5-HT-2F; 5-HT2B"
                     /note="TM helix 3 [structural motif]; Region: TM helix 3"
                     /db_xref="CDD:410628"
     misc_feature    716..781
                     /gene="Htr2b"
                     /gene_synonym="5-HT-2B; 5-HT-2F; 5-HT2B"
                     /note="propagated from UniProtKB/Swiss-Prot (P30994.1);
                     transmembrane region"
     misc_feature    782..790
                     /gene="Htr2b"
                     /gene_synonym="5-HT-2B; 5-HT-2F; 5-HT2B"
                     /note="propagated from UniProtKB/Swiss-Prot (P30994.1);
                     Region: DRY motif, important for ligand-induced
                     conformation changes.
                     /evidence=ECO:0000250|UniProtKB:P41595"
     misc_feature    842..904
                     /gene="Htr2b"
                     /gene_synonym="5-HT-2B; 5-HT-2F; 5-HT2B"
                     /note="propagated from UniProtKB/Swiss-Prot (P30994.1);
                     transmembrane region"
     misc_feature    848..898
                     /gene="Htr2b"
                     /gene_synonym="5-HT-2B; 5-HT-2F; 5-HT2B"
                     /note="TM helix 4 [structural motif]; Region: TM helix 4"
                     /db_xref="CDD:410628"
     misc_feature    938..940
                     /gene="Htr2b"
                     /gene_synonym="5-HT-2B; 5-HT-2F; 5-HT2B"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (P30994.1); glycosylation site"
     misc_feature    953..955
                     /gene="Htr2b"
                     /gene_synonym="5-HT-2B; 5-HT-2F; 5-HT2B"
                     /note="Hydrophobic barrier that decreases the speed of
                     ligand binding and dissociation.
                     /evidence=ECO:0000250|UniProtKB:P41595; propagated from
                     UniProtKB/Swiss-Prot (P30994.1); other site"
     misc_feature    962..973
                     /gene="Htr2b"
                     /gene_synonym="5-HT-2B; 5-HT-2F; 5-HT2B"
                     /note="propagated from UniProtKB/Swiss-Prot (P30994.1);
                     Region: [DE]RFG motif, may stabilize a conformation that
                     preferentially activates signaling via beta-arrestin
                     family members. /evidence=ECO:0000250|UniProtKB:P41595"
     misc_feature    971..1042
                     /gene="Htr2b"
                     /gene_synonym="5-HT-2B; 5-HT-2F; 5-HT2B"
                     /note="TM helix 5 [structural motif]; Region: TM helix 5"
                     /db_xref="CDD:410628"
     misc_feature    977..1045
                     /gene="Htr2b"
                     /gene_synonym="5-HT-2B; 5-HT-2F; 5-HT2B"
                     /note="propagated from UniProtKB/Swiss-Prot (P30994.1);
                     transmembrane region"
     misc_feature    1295..1363
                     /gene="Htr2b"
                     /gene_synonym="5-HT-2B; 5-HT-2F; 5-HT2B"
                     /note="TM helix 6 [structural motif]; Region: TM helix 6"
                     /db_xref="CDD:410628"
     misc_feature    1301..1363
                     /gene="Htr2b"
                     /gene_synonym="5-HT-2B; 5-HT-2F; 5-HT2B"
                     /note="propagated from UniProtKB/Swiss-Prot (P30994.1);
                     transmembrane region"
     misc_feature    1388..1390
                     /gene="Htr2b"
                     /gene_synonym="5-HT-2B; 5-HT-2F; 5-HT2B"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (P30994.1); glycosylation site"
     misc_feature    1403..1480
                     /gene="Htr2b"
                     /gene_synonym="5-HT-2B; 5-HT-2F; 5-HT2B"
                     /note="TM helix 7 [structural motif]; Region: TM helix 7"
                     /db_xref="CDD:410628"
     misc_feature    1409..1474
                     /gene="Htr2b"
                     /gene_synonym="5-HT-2B; 5-HT-2F; 5-HT2B"
                     /note="propagated from UniProtKB/Swiss-Prot (P30994.1);
                     transmembrane region"
     misc_feature    1454..1468
                     /gene="Htr2b"
                     /gene_synonym="5-HT-2B; 5-HT-2F; 5-HT2B"
                     /note="propagated from UniProtKB/Swiss-Prot (P30994.1);
                     Region: NPxxY motif, important for ligand-induced
                     conformation changes and signaling.
                     /evidence=ECO:0000250|UniProtKB:P41595"
     misc_feature    1760..1768
                     /gene="Htr2b"
                     /gene_synonym="5-HT-2B; 5-HT-2F; 5-HT2B"
                     /note="propagated from UniProtKB/Swiss-Prot (P30994.1);
                     Region: PDZ-binding.
                     /evidence=ECO:0000250|UniProtKB:P41595"
     exon            681..881
                     /gene="Htr2b"
                     /gene_synonym="5-HT-2B; 5-HT-2F; 5-HT2B"
                     /inference="alignment:Splign:2.1.0"
     exon            882..2102
                     /gene="Htr2b"
                     /gene_synonym="5-HT-2B; 5-HT-2F; 5-HT2B"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      

gctgaggctccgagaagtcaggaggaggatctgtcaggggagggagtccagctgaacagacacaggacagcaggcttaggaaagccagaagcagacaagcatcttctgaaatctaagcctctagaaggactagaatctggatgtcttacctgcaaacatggacagatatgtacacagtcccatcttggagaacctgaatctttttagaagaaagaaggccaccttggctgggagtgtctggaggataccatgctttgcaaaagcagatgacctgctagcaactgaccatgctgaccactgtctggaactggactgagtcacagaaaggcgaatggcttcatcttataaaatgtctgaacaaagcacaatttctgagcacattttgcagaaaacatgtgatcacctgatcttgactgaccgttctggattaaaggcagaatcagcagcagaggaaatgaagcagactgccgagaaccaggggaatacagtgcactgggcagctctcctgatcttcgcggtaataatccccaccattggcgggaacatcctggttattctggctgtttcactggagaaaaggctgcagtacgctaccaactactttctaatgtccttggcggtggctgatttgctggttggattgtttgtgatgccgattgctctcttaacaatcatgtttgaggctacatggcccctcccactggccctgtgtcctgcctggttattccttgatgttctcttttcaactgcctccatcatgcatctctgtgccatttccctggatcgctatatagccatcaaaaagccaattcaggccaatcagtgcaattcccggactactgcattcgtcaagattacggtggtatggttaatttcaataggcatcgccatcccagtccctattaaaggaatagaggctgatgtggtcaacgcacacaacatcacctgtgagctgacaaaggaccgctttggcagtttcatgctctttgggtcactggctgctttctttgcacctctcaccatcatgatagtcacctactttctcaccattcacgctttgcggaagaaagcttacttggtcagaaacaggccacctcaacgcctaacacggtggactgtgtccacagttctccaaagggaagactcatccttttcatcaccagaaaagatggcgatgctggatggctctcacaaggataaaattctacctaactcaattgatgagacactgatgagaagaatgtcctcagcaggaaaaaaaccagcccagaccatttctaatgaacagagagcctcaaaggtccttggaattgtgtttctcttctttctgcttatgtggtgcccctttttcattacaaacgtaactttagctctgtgtgattcctgcaaccagactactctcaaaacactcctgcagatatttgtgtgggtaggctacgtttcctcgggagtgaatcctttgatctataccctcttcaataagacatttcgggaagcatttggcaggtacatcacctgcaattaccaggccacaaagtcagtaaaagtgcttagaaagtgttctagtacactctattttgggaattcaatggtagaaaactctaaatttttcacaaaacatggaattcgaaatgggatcaaccctgccatgtaccagagcccagtaaggctccgaagttcaaccattcagtcttcatccatcattctcctcaatacatttctcactgaaaacgatggtgacaaagtagaagaccaagtcagctacatatagtggaatggggcagccctcatctgactgagggaggggatgaggaggacgcaagcaaaccaaggaaaaggcaagagtgaagcactaaggttgtccagtttccttatctaaacaaactcaacgcacgggtatagtagttccgtatggctacaaacaaaagcattccctactctggtattcaaatggaacaaaattaaataagtggatatacttcagtctttaaaaagaaaagaaggggttggggatttagctcagtggtagagagtttgcctagtaagtgcaaggccctgggttcggttctcagctccagaaaaaaagaaaataaaaaaaaaaa

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