LOCUS       NM_001393886            1796 bp    mRNA    linear   ROD 10-SEP-2023
DEFINITION  Rattus norvegicus sequestosome 1 (Sqstm1), transcript variant 5,
            mRNA.
ACCESSION   NM_001393886 XM_006246215
VERSION     NM_001393886.1
KEYWORDS    RefSeq.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 1796)
  AUTHORS   Chernyshova EV, Gureev AP, Sadovnikova IS, Plotnikov EY, Silachev
            DN, Zorov DB and Popov VN.
  TITLE     The Relationship of p62 Gene Expression with Integrity of
            Mitochondrial DNA and the Level of Lipid Peroxidation Products in
            Skeletal Muscles of Rats of Different Ages Exposed to Different
            Feeding Protocols
  JOURNAL   Bull Exp Biol Med 175 (2), 245-248 (2023)
   PUBMED   37466855
  REMARK    GeneRIF: The Relationship of p62 Gene Expression with Integrity of
            Mitochondrial DNA and the Level of Lipid Peroxidation Products in
            Skeletal Muscles of Rats of Different Ages Exposed to Different
            Feeding Protocols.
REFERENCE   2  (bases 1 to 1796)
  AUTHORS   Zeng T, Zhang S, He Y, Liu Z and Cheng Q.
  TITLE     MiR-361-5p promotes oxygen-glucose deprivation/re-oxygenation
            induced neuronal injury by negatively regulating SQSTM1 in vitro
  JOURNAL   Metab Brain Dis 36 (8), 2359-2368 (2021)
   PUBMED   34581931
  REMARK    GeneRIF: MiR-361-5p promotes oxygen-glucose
            deprivation/re-oxygenation induced neuronal injury by negatively
            regulating SQSTM1 in vitro.
REFERENCE   3  (bases 1 to 1796)
  AUTHORS   Feng X, Chen L, Guo W, Zhang Y, Lai X, Shao L and Li Y.
  TITLE     Graphene oxide induces p62/SQSTM-dependent apoptosis through the
            impairment of autophagic flux and lysosomal dysfunction in PC12
            cells
  JOURNAL   Acta Biomater 81, 278-292 (2018)
   PUBMED   30273743
  REMARK    GeneRIF: Graphene oxide induces p62/SQSTM-dependent apoptosis
            through the impairment of autophagic flux and lysosomal dysfunction
            in PC12 cells.
REFERENCE   4  (bases 1 to 1796)
  AUTHORS   Bresciani A, Spiezia MC, Boggio R, Cariulo C, Nordheim A, Altobelli
            R, Kuhlbrodt K, Dominguez C, Munoz-Sanjuan I, Wityak J, Fodale V,
            Marchionini DM and Weiss A.
  TITLE     Quantifying autophagy using novel LC3B and p62 TR-FRET assays
  JOURNAL   PLoS One 13 (3), e0194423 (2018)
   PUBMED   29554128
  REMARK    GeneRIF: Quantifying autophagy using novel LC3B and p62 TR-FRET
            assays.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 1796)
  AUTHORS   Chung H, Choi J and Park S.
  TITLE     Ghrelin protects adult rat hippocampal neural stem cells from
            excessive autophagy during oxygen-glucose deprivation
  JOURNAL   Endocr J 65 (1), 63-73 (2018)
   PUBMED   29057768
  REMARK    GeneRIF: Data suggest that neuroprotection via ghrelin involves
            up-regulation of Bcl-2 (B cell lymphoma 2 associated oncogene) and
            p-62/Sqstm1 (ubiquitin-binding protein p62) plus down-regulation of
            LC3-II (microtubule-associated protein 1 light chain 3) and
            Beclin-1. Studies were conducted in adult hippocampal neural stem
            cells in primary culture.
REFERENCE   6  (bases 1 to 1796)
  AUTHORS   Wooten MW, Seibenhener ML, Mamidipudi V, Diaz-Meco MT, Barker PA
            and Moscat J.
  TITLE     The atypical protein kinase C-interacting protein p62 is a scaffold
            for NF-kappaB activation by nerve growth factor
  JOURNAL   J Biol Chem 276 (11), 7709-7712 (2001)
   PUBMED   11244088
REFERENCE   7  (bases 1 to 1796)
  AUTHORS   Kuusisto E, Suuronen T and Salminen A.
  TITLE     Ubiquitin-binding protein p62 expression is induced during
            apoptosis and proteasomal inhibition in neuronal cells
  JOURNAL   Biochem Biophys Res Commun 280 (1), 223-228 (2001)
   PUBMED   11162503
REFERENCE   8  (bases 1 to 1796)
  AUTHORS   Gong J, Xu J, Bezanilla M, van Huizen R, Derin R and Li M.
  TITLE     Differential stimulation of PKC phosphorylation of potassium
            channels by ZIP1 and ZIP2
  JOURNAL   Science 285 (5433), 1565-1569 (1999)
   PUBMED   10477520
REFERENCE   9  (bases 1 to 1796)
  AUTHORS   Nakaso K, Kitayama M, Ishii T, Bannai S, Yanagawa T, Kimura K,
            Nakashima K, Ohama E and Yamada K.
  TITLE     Effects of kainate-mediated excitotoxicity on the expression of rat
            counterparts of A170 and MSP23 stress proteins in the brain
  JOURNAL   Brain Res Mol Brain Res 69 (2), 155-163 (1999)
   PUBMED   10366737
REFERENCE   10 (bases 1 to 1796)
  AUTHORS   Joung I, Strominger JL and Shin J.
  TITLE     Molecular cloning of a phosphotyrosine-independent ligand of the
            p56lck SH2 domain
  JOURNAL   Proc Natl Acad Sci U S A 93 (12), 5991-5995 (1996)
   PUBMED   8650207
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JACYVU010000219.1.
            
            On Mar 19, 2021 this sequence version replaced XM_006246215.3.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR8487231.146512.1 [ECO:0000332]
            ##Evidence-Data-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-233               JACYVU010000219.1  22569752-22569984   c
            234-329             JACYVU010000219.1  22567698-22567793   c
            330-556             JACYVU010000219.1  22567230-22567456   c
            557-698             JACYVU010000219.1  22566986-22567127   c
            699-919             JACYVU010000219.1  22561454-22561674   c
            920-1001            JACYVU010000219.1  22561170-22561251   c
            1002-1796           JACYVU010000219.1  22558831-22559625   c
FEATURES             Location/Qualifiers
     source          1..1796
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="10"
                     /map="10q22"
     gene            1..1796
                     /gene="Sqstm1"
                     /gene_synonym="Osi; ZIP; ZIP3"
                     /note="sequestosome 1"
                     /db_xref="GeneID:113894"
                     /db_xref="RGD:69287"
     exon            1..233
                     /gene="Sqstm1"
                     /gene_synonym="Osi; ZIP; ZIP3"
                     /inference="alignment:Splign:2.1.0"
     misc_feature    23..25
                     /gene="Sqstm1"
                     /gene_synonym="Osi; ZIP; ZIP3"
                     /note="upstream in-frame stop codon"
     CDS             35..1159
                     /gene="Sqstm1"
                     /gene_synonym="Osi; ZIP; ZIP3"
                     /note="isoform 5 is encoded by transcript variant 5;
                     oxidative stress induced; protein kinase
                     C-zeta-interacting protein; ubiquitin-binding protein p62;
                     PKC-zeta-interacting protein"
                     /codon_start=1
                     /product="sequestosome-1 isoform 5"
                     /protein_id="NP_001380815.1"
                     /db_xref="GeneID:113894"
                     /db_xref="RGD:69287"
                     /translation="

MASLTVKAYLLGKEEAAREIRRFSFCFSPEPEAEAAAGPGPCERLLSRVAVLFPALRPGGFQAHYRDEDGDLVAFSSDEELTMAMSYVKDDIFRIYIKEKKECRREHRPPCAQEARSMVHPNVICDGCNGPVVGTRYKCSVCPDYDLCSVCEGKGLHREHSKLIFPNPFGHLSDSFSHSRWLRKLKHGHFGWPGWEMGPPGNWSPRPPRAGDGRPCPTAESGIEVDIDVEHGGKRSRLTPTSAESSSTGTEDKSGTQPSSCSSEVSKPDGAGEGPAQSLTEQMKKIALESVGQPEELMESDNCSGGDDDWTHLSSKEVDPSTEADPRLIESLSQMLSMGFSDEGGWLTRLLQTKNYDIGAALDTIQYSKHPPPL"

     misc_feature    44..334
                     /gene="Sqstm1"
                     /gene_synonym="Osi; ZIP; ZIP3"
                     /note="The PB1 domain is an essential part of p62 scaffold
                     protein (alias sequestosome 1,SQSTM) involved in cell
                     signaling, receptor internalization, and protein turnover.
                     The PB1 domain is a modular domain mediating specific
                     protein-protein interaction which...; Region: PB1_p62;
                     cd06402"
                     /db_xref="CDD:99723"
     misc_feature    401..523
                     /gene="Sqstm1"
                     /gene_synonym="Osi; ZIP; ZIP3"
                     /note="Zinc finger, ZZ type. Zinc finger present in
                     Drosophila ref(2)P, NBR1, Human sequestosome 1 and related
                     proteins. The ZZ motif coordinates two zinc ions and most
                     likely participates in ligand binding or molecular
                     scaffolding. Drosophila ref(2)P appears...; Region:
                     ZZ_NBR1_like; cd02340"
                     /db_xref="CDD:239080"
     misc_feature    order(407..409,416..418,449..451,458..460,476..478,
                     485..487,503..505,512..514)
                     /gene="Sqstm1"
                     /gene_synonym="Osi; ZIP; ZIP3"
                     /note="Zinc-binding sites [ion binding]; other site"
                     /db_xref="CDD:239080"
     misc_feature    order(407..409,416..418,476..478,485..487)
                     /gene="Sqstm1"
                     /gene_synonym="Osi; ZIP; ZIP3"
                     /note="zinc cluster 1 [ion binding]; other site"
                     /db_xref="CDD:239080"
     misc_feature    order(410..412,440..442,446..448,464..466,470..472)
                     /gene="Sqstm1"
                     /gene_synonym="Osi; ZIP; ZIP3"
                     /note="putative charged binding surface [active]"
                     /db_xref="CDD:239080"
     misc_feature    order(449..451,458..460,503..505,512..514)
                     /gene="Sqstm1"
                     /gene_synonym="Osi; ZIP; ZIP3"
                     /note="zinc cluster 2 [ion binding]; other site"
                     /db_xref="CDD:239080"
     misc_feature    1001..1156
                     /gene="Sqstm1"
                     /gene_synonym="Osi; ZIP; ZIP3"
                     /note="UBA domain-like superfamily; Region: UBA_like_SF;
                     cl21463"
                     /db_xref="CDD:451254"
     misc_feature    order(1046..1054,1061..1072,1079..1084,1091..1093,
                     1124..1132)
                     /gene="Sqstm1"
                     /gene_synonym="Osi; ZIP; ZIP3"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]; other site"
                     /db_xref="CDD:270505"
     exon            234..329
                     /gene="Sqstm1"
                     /gene_synonym="Osi; ZIP; ZIP3"
                     /inference="alignment:Splign:2.1.0"
     exon            330..556
                     /gene="Sqstm1"
                     /gene_synonym="Osi; ZIP; ZIP3"
                     /inference="alignment:Splign:2.1.0"
     exon            557..698
                     /gene="Sqstm1"
                     /gene_synonym="Osi; ZIP; ZIP3"
                     /inference="alignment:Splign:2.1.0"
     exon            699..919
                     /gene="Sqstm1"
                     /gene_synonym="Osi; ZIP; ZIP3"
                     /inference="alignment:Splign:2.1.0"
     exon            920..1001
                     /gene="Sqstm1"
                     /gene_synonym="Osi; ZIP; ZIP3"
                     /inference="alignment:Splign:2.1.0"
     exon            1002..1796
                     /gene="Sqstm1"
                     /gene_synonym="Osi; ZIP; ZIP3"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      

gcccagctgtttcctccgtacctagtctgcggttatggcttcgctcacggtgaaggcctatctactgggcaaggaggaggcggcccgcgagatccgccgcttcagcttctgcttcagcccggagccggaggcggaagccgcggctggcccggggccctgcgagaggctgctgagccgggtggctgtgctgttccccgcgctgcggcctggaggctttcaggcgcactaccgcgatgaggatggggacttggtcgccttctccagtgatgaggaactgacaatggccatgtcctatgtgaaagatgacatcttccgcatctacattaaagagaagaaggagtgccggcgggaacatcgccccccatgtgctcaggaggcacgaagcatggtgcaccccaacgtgatttgtgatggttgcaatgggcctgtggtgggaactcgctataagtgcagtgtgtgccccgactacgacctgtgcagcgtctgcgaggggaagggcctgcacagggagcacagcaagctcatctttcccaacccctttggccacctctctgatagcttctctcatagccgctggcttcggaagctgaaacatgggcactttggctggcctggctgggagatgggcccaccagggaactggagcccacgtcctcctcgcgcaggggatggtcgcccttgccccacagctgagtcgggcatcgaggttgacattgatgtggaacatggagggaagagaagccgcctgacacccacctctgcagaaagttccagcacaggcacagaagataagagtggtactcagccaagcagctgctcttcggaagtcagcaaacctgacggggccggggagggccctgctcagtctctgacagagcagatgaagaagatagccttggagtcggtgggacagccagaggaactgatggagtcggataactgctcaggaggggatgacgactggacgcatttgtcttctaaagaagtggacccatccacagaggctgatccccggctgattgagtcactctctcagatgctgtccatgggtttctcggatgaaggcggctggctcaccaggctcctacagaccaagaattatgacatcggggctgctctggacacgatccagtattcaaagcaccctccaccattgtgacagtgctgtggccaagtcccacaacccacctcccttgtcttctagttgcatcatgtagagtagcagggcttctaaggcccagtgtcttggcattcttctagaaccttcaggtgggactgtgaggccttcttaggcagtaggaaagtgcatgagaagagagtctgagtgtgcacatgctgacccctgagcacagatccaagcagctgtggctgggcttccgctgctttccctcggcctggcctttgccagggagctgtggagtcatgctgcactccacttgctcttgccgccagcattgcacctgcagtccatcatccctgcctgataaccttctgtgttttctttattaaaagggattagtacaactgatagttattttcaacaaataaagccattatgtttagagggggactgtcctcagtgagtgaaaggtggcaggctaccggggcctacggctcctgggtgatggagaacttaggtgaagccaactgaaagatcttatcattcactgtgcccccttttaatggccagtttagtttttgtgtctaatgttaatgcttaaagtacaaaccccctatgtagcttcctctgacttgatttttaagtaacctgtaataaagtggcatatgcactttaa

//