LOCUS       NM_001107276            4899 bp    mRNA    linear   ROD 22-MAR-2023
DEFINITION  Rattus norvegicus piwi-like RNA-mediated gene silencing 2 (Piwil2),
            mRNA.
ACCESSION   NM_001107276 XM_001069687 XM_224334
VERSION     NM_001107276.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 4899)
  AUTHORS   Lu Y, Zheng X, Hu W, Bian S, Zhang Z, Tao D, Liu Y and Ma Y.
  TITLE     Cancer/testis antigen PIWIL2 suppresses circadian rhythms by
            regulating the stability and activity of BMAL1 and CLOCK
  JOURNAL   Oncotarget 8 (33), 54913-54924 (2017)
   PUBMED   28903391
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4899)
  AUTHORS   Wenda JM, Homolka D, Yang Z, Spinelli P, Sachidanandam R, Pandey RR
            and Pillai RS.
  TITLE     Distinct Roles of RNA Helicases MVH and TDRD9 in PIWI
            Slicing-Triggered Mammalian piRNA Biogenesis and Function
  JOURNAL   Dev Cell 41 (6), 623-637 (2017)
   PUBMED   28633017
REFERENCE   3  (bases 1 to 4899)
  AUTHORS   Sivagurunathan S, Palanisamy K, Arunachalam JP and Chidambaram S.
  TITLE     Possible role of HIWI2 in modulating tight junction proteins in
            retinal pigment epithelial cells through Akt signaling pathway
  JOURNAL   Mol Cell Biochem 427 (1-2), 145-156 (2017)
   PUBMED   28025795
REFERENCE   4  (bases 1 to 4899)
  AUTHORS   Barau J, Teissandier A, Zamudio N, Roy S, Nalesso V, Herault Y,
            Guillou F and Bourc'his D.
  TITLE     The DNA methyltransferase DNMT3C protects male germ cells from
            transposon activity
  JOURNAL   Science 354 (6314), 909-912 (2016)
   PUBMED   27856912
REFERENCE   5  (bases 1 to 4899)
  AUTHORS   Yang Z, Chen KM, Pandey RR, Homolka D, Reuter M, Janeiro BK,
            Sachidanandam R, Fauvarque MO, McCarthy AA and Pillai RS.
  TITLE     PIWI Slicing and EXD1 Drive Biogenesis of Nuclear piRNAs from
            Cytosolic Targets of the Mouse piRNA Pathway
  JOURNAL   Mol Cell 61 (1), 138-152 (2016)
   PUBMED   26669262
REFERENCE   6  (bases 1 to 4899)
  AUTHORS   Watanabe T, Totoki Y, Toyoda A, Kaneda M, Kuramochi-Miyagawa S,
            Obata Y, Chiba H, Kohara Y, Kono T, Nakano T, Surani MA, Sakaki Y
            and Sasaki H.
  TITLE     Endogenous siRNAs from naturally formed dsRNAs regulate transcripts
            in mouse oocytes
  JOURNAL   Nature 453 (7194), 539-543 (2008)
   PUBMED   18404146
REFERENCE   7  (bases 1 to 4899)
  AUTHORS   Kuramochi-Miyagawa S, Watanabe T, Gotoh K, Totoki Y, Toyoda A,
            Ikawa M, Asada N, Kojima K, Yamaguchi Y, Ijiri TW, Hata K, Li E,
            Matsuda Y, Kimura T, Okabe M, Sakaki Y, Sasaki H and Nakano T.
  TITLE     DNA methylation of retrotransposon genes is regulated by Piwi
            family members MILI and MIWI2 in murine fetal testes
  JOURNAL   Genes Dev 22 (7), 908-917 (2008)
   PUBMED   18381894
REFERENCE   8  (bases 1 to 4899)
  AUTHORS   Aravin AA, Sachidanandam R, Girard A, Fejes-Toth K and Hannon GJ.
  TITLE     Developmentally regulated piRNA clusters implicate MILI in
            transposon control
  JOURNAL   Science 316 (5825), 744-747 (2007)
   PUBMED   17446352
REFERENCE   9  (bases 1 to 4899)
  AUTHORS   Aravin A, Gaidatzis D, Pfeffer S, Lagos-Quintana M, Landgraf P,
            Iovino N, Morris P, Brownstein MJ, Kuramochi-Miyagawa S, Nakano T,
            Chien M, Russo JJ, Ju J, Sheridan R, Sander C, Zavolan M and Tuschl
            T.
  TITLE     A novel class of small RNAs bind to MILI protein in mouse testes
  JOURNAL   Nature 442 (7099), 203-207 (2006)
   PUBMED   16751777
REFERENCE   10 (bases 1 to 4899)
  AUTHORS   Kuramochi-Miyagawa S, Kimura T, Ijiri TW, Isobe T, Asada N, Fujita
            Y, Ikawa M, Iwai N, Okabe M, Deng W, Lin H, Matsuda Y and Nakano T.
  TITLE     Mili, a mammalian member of piwi family gene, is essential for
            spermatogenesis
  JOURNAL   Development 131 (4), 839-849 (2004)
   PUBMED   14736746
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from CH473951.1.
            
            On or before Oct 4, 2007 this sequence version replaced
            XM_224334.4, XM_001069687.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: mixed/partial sample support SAMD00132266,
                              SAMD00132270 [ECO:0000350]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..4899
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="15"
                     /map="15p11"
     gene            1..4899
                     /gene="Piwil2"
                     /note="piwi-like RNA-mediated gene silencing 2"
                     /db_xref="GeneID:306011"
                     /db_xref="RGD:1306964"
     exon            1..138
                     /gene="Piwil2"
                     /inference="alignment:Splign:2.1.0"
     exon            139..375
                     /gene="Piwil2"
                     /inference="alignment:Splign:2.1.0"
     CDS             184..3099
                     /gene="Piwil2"
                     /note="piwi like homolog 2; piwi-like 2"
                     /codon_start=1
                     /product="piwi-like protein 2"
                     /protein_id="NP_001100746.1"
                     /db_xref="GeneID:306011"
                     /db_xref="RGD:1306964"
                     /translation="

MDPVRPLFRGPTPVHPSQCVRMPGCWPQAPRPLEPAWGRAGPAGRGLVFRKPEESSPPLQPVQKESVGLVSMFRGMGLDTALRAPSKREVPPLGRGVLGRGLSANVVRKDREESRSSLPDPSVLAAGDSKLAEASVGWSRMLGRGSSDVSLLPLGRAAGSIGRGVDKPPSAFGLTARDPPRLPQPPTLSPTSLHSSDPPPVLTIERKEKELLVKQGSKGTPQSLGLNLIKIQCHNEAVYQYHVTFSPNVECKSMRFGMLKDHQSVTGNVTAFDGSILYLPVKLQQLVELKSQRKTDDAEISIKIQLTKILEPCSDLCIPFYNVVFRRVMKLLDMKLVGRNFYDPTSAMVLQQHRLQIWPGYAASIRRTDGGLFLLADVSHKVIRNDSVLDVMHAIYQQNKEHFQDECSKLLVGSIVITRYNNRTYRIDDVDWNKTPKDSFVMSDGKEITFLEYYSKNYGITVKEDDQPLLIHRPSERQNNHGTLLKGEILLLPELSFMTGIPEKMKKDFRAMKDLTQQINLSPKQHHSALECLLQRISQNETANNELTRWGLSLQKDVHKIEGRLLPMERINLRNTSFVTSEDLNWVKEVTRDASILTIPMHFWALFYPKRAMDQARELVNMLEKIAGPIGMRISPPAWVELKDDRIETYIRTIQSLLGVEGKIQMVVCIIMGTRDDLYGAIKKLCCVQSPVPSQVINVRTIGQPTRLRSVAQKILLQMNCKLGGELWGVDIPLKQLMVIGMDVYHDPSRGMRSVVGFVASINLTLTKWYSRVVFQMPHQEIVDSLKLCLVGSLKKYYEVNHCLPEKIVVYRDGVSDGQLKTVANYEIPQLQKCFEAFDNYHPKMVVFVVQKKISTNLYLAAPDHFVTPSPGTVVDHTITSCEWVDFYLLAHHVRQGCGIPTHYICVLNTANLSPDHMQRLTFKLCHMYWNWPGTIRVPAPCKYAHKLAFLSGQILHHEPAIQLCENLFFL"

     misc_feature    1192..1335
                     /gene="Piwil2"
                     /note="Argonaute linker 1 domain; Region: ArgoL1;
                     pfam08699"
                     /db_xref="CDD:430160"
     misc_feature    1336..1689
                     /gene="Piwil2"
                     /note="PAZ domain, Piwi_like subfamily. In multi-cellular
                     organisms, the Piwi protein appears to be essential for
                     the maintenance of germline stem cells. In the Drosophila
                     male germline, Piwi was shown to be involved in the
                     silencing of retrotransposons in the...; Region:
                     PAZ_piwi_like; cd02845"
                     /db_xref="CDD:239211"
     misc_feature    order(1468..1470,1507..1509,1531..1533,1543..1545,
                     1597..1599,1648..1650,1654..1656)
                     /gene="Piwil2"
                     /note="nucleic acid-binding interface [nucleotide
                     binding]; other site"
                     /db_xref="CDD:239211"
     misc_feature    1711..3045
                     /gene="Piwil2"
                     /note="PIWI domain, Piwi-like subfamily found in
                     eukaryotes. This domain is found in Piwi and closely
                     related proteins, where it is believed to perform a
                     crucial role in germline cells, via RNA silencing. RNA
                     silencing refers to a group of related...; Region:
                     Piwi_piwi-like_Euk; cd04658"
                     /db_xref="CDD:240016"
     misc_feature    order(2218..2220,2230..2232,2266..2277,2284..2286,
                     2314..2316,2323..2325,2335..2337,2347..2349)
                     /gene="Piwil2"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240016"
     misc_feature    order(2410..2412,2416..2418,2620..2622,3019..3021)
                     /gene="Piwil2"
                     /note="active site"
                     /db_xref="CDD:240016"
     exon            376..463
                     /gene="Piwil2"
                     /inference="alignment:Splign:2.1.0"
     exon            464..602
                     /gene="Piwil2"
                     /inference="alignment:Splign:2.1.0"
     exon            603..809
                     /gene="Piwil2"
                     /inference="alignment:Splign:2.1.0"
     exon            810..920
                     /gene="Piwil2"
                     /inference="alignment:Splign:2.1.0"
     exon            921..1038
                     /gene="Piwil2"
                     /inference="alignment:Splign:2.1.0"
     exon            1039..1163
                     /gene="Piwil2"
                     /inference="alignment:Splign:2.1.0"
     exon            1164..1244
                     /gene="Piwil2"
                     /inference="alignment:Splign:2.1.0"
     exon            1245..1358
                     /gene="Piwil2"
                     /inference="alignment:Splign:2.1.0"
     exon            1359..1547
                     /gene="Piwil2"
                     /inference="alignment:Splign:2.1.0"
     exon            1548..1632
                     /gene="Piwil2"
                     /inference="alignment:Splign:2.1.0"
     exon            1633..1722
                     /gene="Piwil2"
                     /inference="alignment:Splign:2.1.0"
     exon            1723..1863
                     /gene="Piwil2"
                     /inference="alignment:Splign:2.1.0"
     exon            1864..1977
                     /gene="Piwil2"
                     /inference="alignment:Splign:2.1.0"
     exon            1978..2166
                     /gene="Piwil2"
                     /inference="alignment:Splign:2.1.0"
     exon            2167..2268
                     /gene="Piwil2"
                     /inference="alignment:Splign:2.1.0"
     exon            2269..2385
                     /gene="Piwil2"
                     /inference="alignment:Splign:2.1.0"
     exon            2386..2474
                     /gene="Piwil2"
                     /inference="alignment:Splign:2.1.0"
     exon            2475..2580
                     /gene="Piwil2"
                     /inference="alignment:Splign:2.1.0"
     exon            2581..2834
                     /gene="Piwil2"
                     /inference="alignment:Splign:2.1.0"
     exon            2835..2942
                     /gene="Piwil2"
                     /inference="alignment:Splign:2.1.0"
     exon            2943..4899
                     /gene="Piwil2"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      

agtgtgttggaggaatgcgggtcctggaataggagggaaaggaggtggctcgaggagagagcgagcgagcactcgcttcggggctcagtggcacaagacctaaaaagaaatctaggcaagactccagttggtggaaaggtaacgaattggaacagaaccaacagtcatttctactgcctctcaatggatcctgtccggccattgttcagggggcctactccagtccacccatctcaatgtgttcggatgccaggctgttggccgcaagctcctagacctttggaaccagcttggggtagggcaggacctgcaggcagaggccttgtgtttagaaagccagaggaatccagcccaccactccagccagtacaaaaggagtctgtgggtttggtgtccatgtttcgtggcatgggtcttgacacagcattgcgggccccttcaaaacgagaagtgcctcctttaggcagaggagttctaggtcgaggcttgtctgctaacgtagtccgcaaggacagagaagaatcccgttcctctttgcctgatccttcggtgctggcagctggggacagcaagctggcagaggcttctgttggatggagtagaatgctgggaagaggtagttctgatgtctctttgttaccactgggaagagcagctggtagtataggcagaggagtggacaaacctcccagtgcctttggccttactgctcgggatccccctcggctgccccagcctccaactctgtctccaacttcactgcactcctctgatcctcctccagtcctgactatagagcgaaaggaaaaagagcttttggtcaagcaaggatcaaaaggaacacctcagtctttaggactgaacctcatcaaaatccagtgtcataacgaagcagtttatcaatatcatgtaactttcagccccaatgtggagtgcaaaagcatgaggtttggcatgttgaaggaccaccaatctgtcactggaaacgttactgcttttgatggctctattctttatcttcctgttaagcttcaacaacttgttgagttaaaaagccagagaaaaactgatgatgccgagatcagcatcaagattcagctgacaaagatcctggagccatgttccgacttgtgcattcccttctacaatgttgtcttccggcgggtaatgaaacttttggatatgaagcttgtggggagaaacttctatgatcctacaagtgccatggtactgcagcaacacagattgcagatctggcctggctatgcagctagtatccggaggacagatgggggtctcttcttgctcgctgatgtctctcataaggtcattcggaatgactctgtactggatgtcatgcatgctatctaccagcagaacaaggagcacttccaggacgagtgcagcaagcttctggtgggcagcattgtcatcacacgctacaacaaccgtacctaccgcattgacgatgtggactggaacaagacccctaaagacagctttgtaatgtcggatgggaaggagatcacattcctggagtactacagtaaaaactatgggatcacagttaaagaagatgaccagccgctgctcatccaccggcccagtgagagacagaataaccatggcacgttgctgaagggtgagatcctgctgctgcctgagctttccttcatgacggggattcctgagaagatgaagaaggacttcagggccatgaaggacttgactcagcagattaacctgagccccaagcagcaccacagtgctttggaatgcctgctgcagagaatttcacaaaatgagacagccaacaacgagctgacccgctgggggctcagtctgcaaaaagatgtccacaagatcgaaggccgtcttctaccaatggagaggattaacttaaggaatacttcatttgtcacatcggaggacctgaactgggttaaggaggtgaccagagatgcttccattctgactattcccatgcatttctgggcactcttttatccaaagagagcaatggaccaagccagagaactggttaacatgttggaaaagattgctggccccattggcatgcgcataagccccccagcctgggttgaactgaaggatgaccgaatagagacctatatcaggaccattcagtccttactgggagttgaggggaagatacaaatggtcgtttgcatcatcatgggcacacgggatgatctctatggggccatcaagaaactgtgctgtgtgcagtccccagtgccctcacaggtcatcaatgtccgaaccattggtcaacccaccaggcttcggagtgtggctcagaaaattttacttcagatgaactgtaaactgggtggtgagctctggggagtagatattccactgaaacaattaatggtgattgggatggatgtgtaccacgaccccagcagaggcatgcgctctgtggttggctttgttgccagcataaatctcacactcaccaaatggtactcgagggtggtgttccagatgccgcatcaggagattgtggacagcctgaagctctgcctggtgggctccttgaaaaagtattatgaggtgaaccactgtctcccagagaagattgtggtgtaccgagatggagtgtctgatggccagctaaagacagttgccaactatgagatccctcagctgcagaagtgctttgaagcctttgataactaccatcccaagatggtggtatttgtagttcagaagaaaatcagcaccaatctgtaccttgctgctcctgaccactttgtgaccccctcccctgggactgtggttgatcataccataaccagctgtgagtgggtggatttctaccttcttgctcatcatgtgcgacagggctgtggcatacctacacactacatctgtgttctgaacactgcaaatctgagccctgatcacatgcagaggctgactttcaaactatgccacatgtactggaattggcctggtaccatccgagttccagctccttgcaagtatgcccacaagctagctttcctgtccggacagattttgcatcatgaaccagccatccagctatgtgagaacctgttcttcctgtaactgggaacttggacaccagctgcaaggagcaactggattccagctcagctcattcttacagaatcaacagaaatggcagtggagttttacgtttgcattttctctttctccatccttgtagaattagatttctgttcttcttttaaccctgatattatagtagagtggtgtggtacatgcctgttactccagcacatgggagactgaagcagaaggatctttagttcaaggccagcctggactacatggaaagttccaagctagccaagattatagagtgagaccctatctcaaaagacaaaaccaaaccactgtccccacaaagcccacaacaaaaccaaagcctggtgtcaaggaagcaagtttctgagtagcagccagcagctgccttgatggagcagagtgatgttagcagtgccgctgctctgccgagcatgggcctacggacatgtccagtccccctaccaaataagtgaaggacataaaaaacagctcccaaggcagcatgagcatcagtttataaaccagagtttatgcctacaagatcatcctatttctgggccagaagtgacatgaatgaagtagtcattgcagatcttaaaggagagagttactatgttcagagagagttagtgtttcagagagagcagctgtttgcatgcatggaagatgtaggaaaaccagtgtaaggcacagtcaggtgagctgacttgacccaggccagtgagtttggccactccttggcctcctgagagtccagctagagctgcttttctggaccttttagtcatttacgaccatcagattgctttcctgcagcctgactgacacattcatgtgcacatacacacttttgatctttgtgactaatgtgaaataaattcagttaggacattcagcagacctaggaacattctggatggagtataggtttttttctgcaaaaatctgtaaatgagattatgcaagagtctcttccagctgtgggctggtgttgcttggaaattttcaaaatcccaaagtttcatgcttcccaaagttggcttggacaaatgtgatgctctcacctgagcctagacatgtaggaagttttcctagcgccagtgggcttctgtatttgggtgaagcactggttttctgcgttttttttttcccttctgtttcagaatcttcaagtttcttcaggcttaatgaaccatcccttgctttactgggttttaaaagctaattttacttaacctcttcaaatttgtgtgtatctatttatgttttggtgtggtggatggatggtaggggactgagcagaaattcagtgtgctaggagagcctcactgggaagtactgaagagccacagggtctgtgggagtcagtgttagcactcaccaagccaggccaggccttgggtttgaaggcaacaccagcaaagcctttagttcagtatagcacccagaggttggcgagggataagtgttggggtcttcaaatacaacccctggtgtttggagtctgtgaccaaggccagactgagacctgaaaggtaaaagccataggattaaacagagcagactgaaaaatgagtctttaggttgaacacattccactttctggatttctctgtgagtattttgagtttgtctttagacgttgctattcctagcactccttttctctttaggtagaacagacacttaaccataatgccagaatgtactttctatacctgatgtccttgccttgtttttcagtttcagggccaggtaattgggccctgtggtgtaaaataaaaacagtgtatgtgt

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