LOCUS       NM_153178              13793 bp    mRNA    linear   ROD 08-APR-2024
DEFINITION  Mus musculus argonaute RISC catalytic subunit 2 (Ago2), mRNA.
ACCESSION   NM_153178
VERSION     NM_153178.5
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 13793)
  AUTHORS   Li,J., Yao,H., Zhao,F., An,J., Wang,Q., Mu,J., Liu,Z., Zou,M.H. and
            Xie,Z.
  TITLE     Pycard deficiency inhibits microRNA maturation and prevents
            neointima formation by promoting chaperone-mediated autophagic
            degradation of AGO2/argonaute 2 in adipose tissue
  JOURNAL   Autophagy 20 (3), 629-644 (2024)
   PUBMED   37963060
  REMARK    GeneRIF: Pycard deficiency inhibits microRNA maturation and
            prevents neointima formation by promoting chaperone-mediated
            autophagic degradation of AGO2/argonaute 2 in adipose tissue.
REFERENCE   2  (bases 1 to 13793)
  AUTHORS   Sala,L., Kumar,M., Prajapat,M., Chandrasekhar,S., Cosby,R.L., La
            Rocca,G., Macfarlan,T.S., Awasthi,P., Chari,R., Kruhlak,M. and
            Vidigal,J.A.
  TITLE     AGO2 silences mobile transposons in the nucleus of quiescent cells
  JOURNAL   Nat Struct Mol Biol 30 (12), 1985-1995 (2023)
   PUBMED   37985687
  REMARK    GeneRIF: AGO2 silences mobile transposons in the nucleus of
            quiescent cells.
REFERENCE   3  (bases 1 to 13793)
  AUTHORS   Feng,Z. and Wu,J.
  TITLE     hsa_circ_0129047 Upregulates LYVE1 to Inhibit Hepatocellular
            Carcinoma Progression by Sponging miR-492
  JOURNAL   Dis Markers 2023, 6978234 (2023)
   PUBMED   37810197
  REMARK    GeneRIF: hsa_circ_0129047 Upregulates LYVE1 to Inhibit
            Hepatocellular Carcinoma Progression by Sponging miR-492.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 13793)
  AUTHORS   Li,Z., Jin,T., Yang,R., Guo,J., Niu,Z., Gao,H., Song,X., Zhang,Q.,
            Ning,Z., Ren,L., Wang,Y., Fan,X., Liang,H., Li,T. and He,W.
  TITLE     Long non-coding RNA PFI inhibits apoptosis of alveolar epithelial
            cells to alleviate lung injury via miR-328-3p/Creb1 axis
  JOURNAL   Exp Cell Res 430 (1), 113685 (2023)
   PUBMED   37330182
  REMARK    GeneRIF: Long non-coding RNA PFI inhibits apoptosis of alveolar
            epithelial cells to alleviate lung injury via miR-328-3p/Creb1
            axis.
REFERENCE   5  (bases 1 to 13793)
  AUTHORS   Shui,B., Beyett,T.S., Chen,Z., Li,X., La Rocca,G., Gazlay,W.M.,
            Eck,M.J., Lau,K.S., Ventura,A. and Haigis,K.M.
  TITLE     Oncogenic K-Ras suppresses global miRNA function
  JOURNAL   Mol Cell 83 (14), 2509-2523 (2023)
   PUBMED   37402366
REFERENCE   6  (bases 1 to 13793)
  AUTHORS   Lu,J., Qian,J., Chen,F., Tang,X., Li,C. and Cardoso,W.V.
  TITLE     Differential expression of components of the microRNA machinery
            during mouse organogenesis
  JOURNAL   Biochem Biophys Res Commun 334 (2), 319-323 (2005)
   PUBMED   16036130
REFERENCE   7  (bases 1 to 13793)
  AUTHORS   McKee,A.E., Minet,E., Stern,C., Riahi,S., Stiles,C.D. and
            Silver,P.A.
  TITLE     A genome-wide in situ hybridization map of RNA-binding proteins
            reveals anatomically restricted expression in the developing mouse
            brain
  JOURNAL   BMC Dev Biol 5, 14 (2005)
   PUBMED   16033648
  REMARK    Publication Status: Online-Only
REFERENCE   8  (bases 1 to 13793)
  AUTHORS   Liu,J., Carmell,M.A., Rivas,F.V., Marsden,C.G., Thomson,J.M.,
            Song,J.J., Hammond,S.M., Joshua-Tor,L. and Hannon,G.J.
  TITLE     Argonaute2 is the catalytic engine of mammalian RNAi
  JOURNAL   Science 305 (5689), 1437-1441 (2004)
   PUBMED   15284456
  REMARK    GeneRIF: essential for mouse development and cells lacking
            Argonaute2 are unable to mount an experimental response to siRNAs
REFERENCE   9  (bases 1 to 13793)
  AUTHORS   Doi,N., Zenno,S., Ueda,R., Ohki-Hamazaki,H., Ui-Tei,K. and Saigo,K.
  TITLE     Short-interfering-RNA-mediated gene silencing in mammalian cells
            requires Dicer and eIF2C translation initiation factors
  JOURNAL   Curr Biol 13 (1), 41-46 (2003)
   PUBMED   12526743
REFERENCE   10 (bases 1 to 13793)
  AUTHORS   Carmell,M.A., Xuan,Z., Zhang,M.Q. and Hannon,G.J.
  TITLE     The Argonaute family: tentacles that reach into RNAi, developmental
            control, stem cell maintenance, and tumorigenesis
  JOURNAL   Genes Dev 16 (21), 2733-2742 (2002)
   PUBMED   12414724
  REMARK    Review article
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC161581.3 and AC118640.8.
            
            On Jul 1, 2022 this sequence version replaced NM_153178.4.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC096465.1, SRR13948564.4563749.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-168               AC161581.3         105098-105265       c
            169-364             AC118640.8         34597-34792
            365-485             AC118640.8         43049-43169
            486-667             AC118640.8         50120-50301
            668-804             AC118640.8         52702-52838
            805-939             AC118640.8         54074-54208
            940-1027            AC118640.8         54628-54715
            1028-1175           AC118640.8         55851-55998
            1176-1295           AC118640.8         56913-57032
            1296-1418           AC118640.8         57163-57285
            1419-1552           AC118640.8         57706-57839
            1553-1737           AC118640.8         59914-60098
            1738-1897           AC118640.8         61666-61825
            1898-1988           AC118640.8         64769-64859
            1989-2183           AC118640.8         67260-67454
            2184-2318           AC118640.8         69341-69475
            2319-2420           AC118640.8         72689-72790
            2421-2620           AC118640.8         73798-73997
            2621-13793          AC118640.8         74146-85318
FEATURES             Location/Qualifiers
     source          1..13793
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="15"
                     /map="15 33.92 cM"
     gene            1..13793
                     /gene="Ago2"
                     /gene_synonym="1110029L17Rik; 2310051F07Rik; Eif2c2;
                     Gerp95; Gm10365; mKIAA4215"
                     /note="argonaute RISC catalytic subunit 2"
                     /db_xref="GeneID:239528"
                     /db_xref="MGI:MGI:2446632"
     exon            1..168
                     /gene="Ago2"
                     /gene_synonym="1110029L17Rik; 2310051F07Rik; Eif2c2;
                     Gerp95; Gm10365; mKIAA4215"
                     /inference="alignment:Splign:2.1.0"
     misc_feature    21..23
                     /gene="Ago2"
                     /gene_synonym="1110029L17Rik; 2310051F07Rik; Eif2c2;
                     Gerp95; Gm10365; mKIAA4215"
                     /note="upstream in-frame stop codon"
     CDS             147..2729
                     /gene="Ago2"
                     /gene_synonym="1110029L17Rik; 2310051F07Rik; Eif2c2;
                     Gerp95; Gm10365; mKIAA4215"
                     /EC_number="3.1.26.n2"
                     /note="argonaute 2; Piwi/Argonaute family protein meIF2C2;
                     eukaryotic translation initiation factor 2C, 2; argonaute
                     RISC catalytic component 2"
                     /codon_start=1
                     /product="protein argonaute-2"
                     /protein_id="NP_694818.3"
                     /db_xref="CCDS:CCDS37098.1"
                     /db_xref="GeneID:239528"
                     /db_xref="MGI:MGI:2446632"
                     /translation="

MYSGAGPVLASPAPTTSPIPGYAFKPPPRPDFGTTGRTIKLQANFFEMDIPKIDIYHYELDIKPEKCPRRVNREIVEHMVQHFKTQIFGDRKPVFDGRKNLYTAMPLPIGRDKVELEVTLPGEGKDRIFKVSIKWVSCVSLQALHDALSGRLPSVPFETIQALDVVMRHLPSMRYTPVGRSFFTASEGCSNPLGGGREVWFGFHQSVRPSLWKMMLNIDVSATAFYKAQPVIEFVCEVLDFKSIEEQQKPLTDSQRVKFTKEIKGLKVEITHCGQMKRKYRVCNVTRRPASHQTFPLQQESGQTVECTVAQYFKDRHKLVLRYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIRATARSAPDRQEEISKLMRSASFNTDPYVREFGIMVKDEMTDVTGRVLQPPSILYGGRNKAIATPVQGVWDMRNKQFHTGIEIKVWAIACFAPQRQCTEVHLKSFTEQLRKISRDAGMPIQGQPCFCKYAQGADSVEPMFRHLKNTYAGLQLVVVILPGKTPVYAEVKRVGDTVLGMATQCVQMKNVQRTTPQTLSNLCLKINVKLGGVNNILLPQGRPPVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDAHPNRYCATVRVQQHRQEIIQDLAAMVRELLIQFYKSTRFKPTRIIFYRDGVSEGQFQQVLHHELLAIREACIKLEKDYQPGITFIVVQKRHHTRLFCTDKNERVGKSGNIPAGTTVDTKITHPTEFDFYLCSHAGIQGTSRPSHYHVLWDDNRFSSDELQILTYQLCHTYVRCTRSVSIPAPAYYAHLVAFRARYHLVDKEHDSAEGSHTSGQSNGRDHQALAKAVQVHQDTLRTMYFA"

     misc_feature    165..2723
                     /gene="Ago2"
                     /gene_synonym="1110029L17Rik; 2310051F07Rik; Eif2c2;
                     Gerp95; Gm10365; mKIAA4215"
                     /note="protein argonaute; Provisional; Region: PLN03202"
                     /db_xref="CDD:215631"
     misc_feature    1080..1097
                     /gene="Ago2"
                     /gene_synonym="1110029L17Rik; 2310051F07Rik; Eif2c2;
                     Gerp95; Gm10365; mKIAA4215"
                     /note="propagated from UniProtKB/Swiss-Prot (Q8CJG0.3);
                     Region: Interaction with guide RNA. /evidence=ECO:0000250"
     misc_feature    1308..1310
                     /gene="Ago2"
                     /gene_synonym="1110029L17Rik; 2310051F07Rik; Eif2c2;
                     Gerp95; Gm10365; mKIAA4215"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q9UKV8; propagated from
                     UniProtKB/Swiss-Prot (Q8CJG0.3); phosphorylation site"
     misc_feature    1323..2600
                     /gene="Ago2"
                     /gene_synonym="1110029L17Rik; 2310051F07Rik; Eif2c2;
                     Gerp95; Gm10365; mKIAA4215"
                     /note="PIWI domain, Argonaute-like subfamily. Argonaute is
                     the central component of the RNA-induced silencing complex
                     (RISC) and related complexes. The PIWI domain is the
                     C-terminal portion of Argonaute and consists of two
                     subdomains, one of which provides the...; Region:
                     Piwi_ago-like; cd04657"
                     /db_xref="CDD:240015"
     misc_feature    1719..1847
                     /gene="Ago2"
                     /gene_synonym="1110029L17Rik; 2310051F07Rik; Eif2c2;
                     Gerp95; Gm10365; mKIAA4215"
                     /note="propagated from UniProtKB/Swiss-Prot (Q8CJG0.3);
                     Region: Interaction with guide RNA. /evidence=ECO:0000250"
     misc_feature    order(1734..1736,1746..1748,1782..1793,1800..1802,
                     1824..1826,1833..1835,1845..1847,1857..1859)
                     /gene="Ago2"
                     /gene_synonym="1110029L17Rik; 2310051F07Rik; Eif2c2;
                     Gerp95; Gm10365; mKIAA4215"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240015"
     misc_feature    1908..1919
                     /gene="Ago2"
                     /gene_synonym="1110029L17Rik; 2310051F07Rik; Eif2c2;
                     Gerp95; Gm10365; mKIAA4215"
                     /note="propagated from UniProtKB/Swiss-Prot (Q8CJG0.3);
                     Region: Interaction with GW182 family members.
                     /evidence=ECO:0000255"
     misc_feature    order(1938..1940,1944..1946,2154..2156,2568..2570)
                     /gene="Ago2"
                     /gene_synonym="1110029L17Rik; 2310051F07Rik; Eif2c2;
                     Gerp95; Gm10365; mKIAA4215"
                     /note="active site"
                     /db_xref="CDD:240015"
     misc_feature    2097..2129
                     /gene="Ago2"
                     /gene_synonym="1110029L17Rik; 2310051F07Rik; Eif2c2;
                     Gerp95; Gm10365; mKIAA4215"
                     /note="propagated from UniProtKB/Swiss-Prot (Q8CJG0.3);
                     Region: Interaction with GW182 family members.
                     /evidence=ECO:0000255"
     misc_feature    2247..2249
                     /gene="Ago2"
                     /gene_synonym="1110029L17Rik; 2310051F07Rik; Eif2c2;
                     Gerp95; Gm10365; mKIAA4215"
                     /note="4-hydroxyproline.
                     /evidence=ECO:0000255|HAMAP-Rule:MF_03031; propagated from
                     UniProtKB/Swiss-Prot (Q8CJG0.3); hydroxylation site"
     misc_feature    2274..2279
                     /gene="Ago2"
                     /gene_synonym="1110029L17Rik; 2310051F07Rik; Eif2c2;
                     Gerp95; Gm10365; mKIAA4215"
                     /note="propagated from UniProtKB/Swiss-Prot (Q8CJG0.3);
                     Region: Interaction with guide RNA. /evidence=ECO:0000250"
     misc_feature    2406..2432
                     /gene="Ago2"
                     /gene_synonym="1110029L17Rik; 2310051F07Rik; Eif2c2;
                     Gerp95; Gm10365; mKIAA4215"
                     /note="propagated from UniProtKB/Swiss-Prot (Q8CJG0.3);
                     Region: Interaction with guide RNA. /evidence=ECO:0000250"
     misc_feature    2517..2585
                     /gene="Ago2"
                     /gene_synonym="1110029L17Rik; 2310051F07Rik; Eif2c2;
                     Gerp95; Gm10365; mKIAA4215"
                     /note="propagated from UniProtKB/Swiss-Prot (Q8CJG0.3);
                     Region: Interaction with guide RNA. /evidence=ECO:0000250"
     misc_feature    2619..2621
                     /gene="Ago2"
                     /gene_synonym="1110029L17Rik; 2310051F07Rik; Eif2c2;
                     Gerp95; Gm10365; mKIAA4215"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q9UKV8; propagated from
                     UniProtKB/Swiss-Prot (Q8CJG0.3); phosphorylation site"
     misc_feature    2631..2633
                     /gene="Ago2"
                     /gene_synonym="1110029L17Rik; 2310051F07Rik; Eif2c2;
                     Gerp95; Gm10365; mKIAA4215"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q9UKV8; propagated from
                     UniProtKB/Swiss-Prot (Q8CJG0.3); phosphorylation site"
     misc_feature    2640..2642
                     /gene="Ago2"
                     /gene_synonym="1110029L17Rik; 2310051F07Rik; Eif2c2;
                     Gerp95; Gm10365; mKIAA4215"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q9UKV8; propagated from
                     UniProtKB/Swiss-Prot (Q8CJG0.3); phosphorylation site"
     misc_feature    2649..2651
                     /gene="Ago2"
                     /gene_synonym="1110029L17Rik; 2310051F07Rik; Eif2c2;
                     Gerp95; Gm10365; mKIAA4215"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q9UKV8; propagated from
                     UniProtKB/Swiss-Prot (Q8CJG0.3); phosphorylation site"
     exon            169..364
                     /gene="Ago2"
                     /gene_synonym="1110029L17Rik; 2310051F07Rik; Eif2c2;
                     Gerp95; Gm10365; mKIAA4215"
                     /inference="alignment:Splign:2.1.0"
     exon            365..485
                     /gene="Ago2"
                     /gene_synonym="1110029L17Rik; 2310051F07Rik; Eif2c2;
                     Gerp95; Gm10365; mKIAA4215"
                     /inference="alignment:Splign:2.1.0"
     exon            486..667
                     /gene="Ago2"
                     /gene_synonym="1110029L17Rik; 2310051F07Rik; Eif2c2;
                     Gerp95; Gm10365; mKIAA4215"
                     /inference="alignment:Splign:2.1.0"
     exon            668..804
                     /gene="Ago2"
                     /gene_synonym="1110029L17Rik; 2310051F07Rik; Eif2c2;
                     Gerp95; Gm10365; mKIAA4215"
                     /inference="alignment:Splign:2.1.0"
     exon            805..939
                     /gene="Ago2"
                     /gene_synonym="1110029L17Rik; 2310051F07Rik; Eif2c2;
                     Gerp95; Gm10365; mKIAA4215"
                     /inference="alignment:Splign:2.1.0"
     exon            940..1027
                     /gene="Ago2"
                     /gene_synonym="1110029L17Rik; 2310051F07Rik; Eif2c2;
                     Gerp95; Gm10365; mKIAA4215"
                     /inference="alignment:Splign:2.1.0"
     exon            1028..1175
                     /gene="Ago2"
                     /gene_synonym="1110029L17Rik; 2310051F07Rik; Eif2c2;
                     Gerp95; Gm10365; mKIAA4215"
                     /inference="alignment:Splign:2.1.0"
     exon            1176..1295
                     /gene="Ago2"
                     /gene_synonym="1110029L17Rik; 2310051F07Rik; Eif2c2;
                     Gerp95; Gm10365; mKIAA4215"
                     /inference="alignment:Splign:2.1.0"
     exon            1296..1418
                     /gene="Ago2"
                     /gene_synonym="1110029L17Rik; 2310051F07Rik; Eif2c2;
                     Gerp95; Gm10365; mKIAA4215"
                     /inference="alignment:Splign:2.1.0"
     exon            1419..1552
                     /gene="Ago2"
                     /gene_synonym="1110029L17Rik; 2310051F07Rik; Eif2c2;
                     Gerp95; Gm10365; mKIAA4215"
                     /inference="alignment:Splign:2.1.0"
     exon            1553..1737
                     /gene="Ago2"
                     /gene_synonym="1110029L17Rik; 2310051F07Rik; Eif2c2;
                     Gerp95; Gm10365; mKIAA4215"
                     /inference="alignment:Splign:2.1.0"
     exon            1738..1897
                     /gene="Ago2"
                     /gene_synonym="1110029L17Rik; 2310051F07Rik; Eif2c2;
                     Gerp95; Gm10365; mKIAA4215"
                     /inference="alignment:Splign:2.1.0"
     exon            1898..1988
                     /gene="Ago2"
                     /gene_synonym="1110029L17Rik; 2310051F07Rik; Eif2c2;
                     Gerp95; Gm10365; mKIAA4215"
                     /inference="alignment:Splign:2.1.0"
     exon            1989..2183
                     /gene="Ago2"
                     /gene_synonym="1110029L17Rik; 2310051F07Rik; Eif2c2;
                     Gerp95; Gm10365; mKIAA4215"
                     /inference="alignment:Splign:2.1.0"
     exon            2184..2318
                     /gene="Ago2"
                     /gene_synonym="1110029L17Rik; 2310051F07Rik; Eif2c2;
                     Gerp95; Gm10365; mKIAA4215"
                     /inference="alignment:Splign:2.1.0"
     exon            2319..2420
                     /gene="Ago2"
                     /gene_synonym="1110029L17Rik; 2310051F07Rik; Eif2c2;
                     Gerp95; Gm10365; mKIAA4215"
                     /inference="alignment:Splign:2.1.0"
     exon            2421..2620
                     /gene="Ago2"
                     /gene_synonym="1110029L17Rik; 2310051F07Rik; Eif2c2;
                     Gerp95; Gm10365; mKIAA4215"
                     /inference="alignment:Splign:2.1.0"
     exon            2621..13793
                     /gene="Ago2"
                     /gene_synonym="1110029L17Rik; 2310051F07Rik; Eif2c2;
                     Gerp95; Gm10365; mKIAA4215"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      

gccgccccggccgcgtcgggtaaacctgttctgcgaggcggccgcgccggggcggatcgagcggccggcggctcctcgcggctcgcggcgtcggggcccgtggagcgcgcggccggccctcggccgcggccctcggcaacgccaccatgtactcgggagccggccccgttcttgcttctcctgctccgacaacatcacccatcccaggatatgccttcaaacctccacctcggccggacttcggcaccaccgggagaacaatcaaactacaggccaatttctttgaaatggacatccccaaaattgacatctatcactatgaattggacatcaaacctgagaaatgccctcggagagtgaacagggaaattgtggagcacatggtccagcactttaaaactcagatcttcggggaccggaagccagtgtttgatggaaggaagaatctgtacacagcaatgccccttccgatcggcagggacaaggtggagctggaggtcacgctcccgggagaaggcaaagatcgcatctttaaggtatccatcaagtgggtgtcgtgcgtgagcctgcaggcgttacacgatgcactttcggggcggctgcccagcgtccccttcgagacgatccaggccctggacgttgtcatgaggcacttaccatccatgaggtacacccctgttggccgttccttcttcactgcatctgaaggctgttccaaccctctgggtgggggcagagaagtgtggtttggcttccatcagtccgtccgaccttctctttggaaaatgatgctgaatattgatgtatcggcaacagcgttttacaaggcacagccagtgatcgagtttgtttgtgaagttttggattttaaaagtattgaagaacaacaaaaacctctgacagattcccaaagggtaaagtttaccaaagaaatcaaaggtctaaaggtggagataacgcactgtggtcagatgaagaggaagtaccgtgtctgcaatgtgacccggcggcctgccagtcaccaaacgttcccactgcagcaggagagtgggcagacagtggagtgtacagtggcccagtacttcaaggacaggcacaagctggttctgcgctacccccacctcccgtgtttacaagtcggacaggagcagaaacacacctaccttcctttggaggtctgtaacatagttgctggacagagatgtataaaaaaattaacagacaatcagacctcaaccatgatcagagcaactgccaggtcagcacctgatcgccaagaggagatcagcaaactgatgcgaagtgcaagtttcaatacagacccatatgttcgtgaatttggaatcatggtgaaagatgagatgacagatgtgactgggcgggttctgcagccgccctccatcctctacgggggcaggaacaaagcaattgccacccctgtccagggtgtgtgggacatgcgaaacaagcagttccacacgggcatcgagatcaaggtgtgggccatcgcctgcttcgctccccagcgccagtgtacagaagtccatctcaagtccttcacagagcaactgaggaagatctcgagagatgctgggatgccgatccagggtcagccctgcttctgtaaatacgcacagggtgcagacagtgtggagcccatgttccgacacctgaagaacacatacgctggcctccagctggtggtggtcatcctgcctggcaaaactcctgtgtatgcggaagtcaagcgtgtgggagacacagtgctggggatggccacacagtgcgtccagatgaagaacgtgcagaggaccacgccacagaccctatccaatctctgcttaaagatcaatgtcaaactgggaggcgtcaacaacatcctgctgccacagggcaggcctccagtgttccagcaacctgtcatcttcctgggagccgatgtcacccacccaccagctggggatgggaagaaaccttctattgctgccgtcgtgggcagcatggacgcacaccccaaccgctactgtgccaccgtgcgcgtgcagcagcaccgacaggagatcatccaggacctggctgccatggtacgagagttgctcattcagttctacaagtccacccgcttcaagcccacccgcatcatcttctaccgcgatggcgtctccgagggccagttccagcaggttctccaccatgagctcctggccatcagagaggcctgcatcaagctggagaaggactatcagccaggaatcacgttcatcgtggtgcagaagcggcaccacacacgcctcttctgcacagacaaaaatgagcgggttgggaagagtgggaacattcccgcaggcacaaccgtggacacgaagatcacccaccccactgagtttgacttctacctgtgcagtcacgccggcatccaggggacaagccgtccttcccactaccacgtgctttgggatgacaatcgtttttcttctgatgagctgcagattctgacctaccagctgtgtcacacctacgtgcgctgcacacgctctgtgtcaatacccgcgccagcttactatgctcacctggtggccttccgagccaggtaccacctggtggataaagaacatgacagcgctgaaggaagccatacctctgggcagagcaacgggcgagatcaccaagcgctggccaaggcggtccaggtccaccaggacacactgcgcaccatgtactttgcttgacctgttgtattgtttagcgattgtgtaccaagttggattcacacgagaccagctacactcagaccaacagatgcccagcccttctgtgacagccagcatcgaacatgagacgtctttggttttattagatcctccgttttccagaatgccttccgtcccagatgtcagacttggattttgagctgcaggcctgtatgagaccccagtgtaggaaacatggtttgccaaacctataagctgctcactgagagtcccgtttcctaaaaacaaaacaacaacaaaatgtcctaaaaccagtctatgaactctcagggctttaaaacactttaatttatttggtcatccaatttacttgtttttaacacctgattctcactgaaaccgatgggctccatctagctgtgaaggctctgagcatgaaagccacaccatttccattggggttttaaagcctggccctaagaagttgtcactaatgttgagttctaagggatgccatgcgccctgcagccccagggcgtatggctgctaggtctccacagtgtctctcgaggtcactgcctgccctcgaggcctgaacaaaggcacagtggccaaggagatggtcacgtgtgtttacggcagtgacaagtccgagagattggcagacaagtgccattttgataaaaatttatttaaaaagagaaaaaaagacaatatactgacagtctaatcataagatttgtcctgtaggactgtgacatttattttccaaagtttctaaagaatacgggtctgtggtgataaatacagtacaatcctttttcactgttaatgtctttaatgtaagagaagaatatatgtatctggtttgcagtattaatgtggtaggtagatgctgtttccctttcgttgacgaggggcgcttcttgtgacctgcttcacatatcaggagctgtgtggagcctggagacagtccacctcttgtggcttttagtgcttaagtagaggaatatgcaactggcttcatgtaccagcgatgccccctcctccctccccacccccagtgtggactgtgtagcctgtacctctggaaggacagtgcgcttgccagtaacctgccgtcctccctgcctcgtccatgtgtgtcgctcagccgatgaagcacaaatggaatttatttattcttccattgctgggaaacaagttcacgtaagagtgcacttctctcagtgtgcagttatggaaggtgggtttcataggatctatgcactgaagtggagagagagctgcccatagcggctctgccagcatgggactggcaggtaggaatggactgtgcccgaggacctgaccgtgggcaaggccagcagggaaggcacagactttctctgggtgttgtttgcttctgggccccagtgtgggttttccagcccagagaccattgaactgagaaggggccactacgagtggctctgcggatggcagcagccacgtcagtgtgtgtgtcctgtggggctttctgtgcttgtcccgaagctgagcaagcatcgggctcgctccgttccacatacgcgtgcgttcccagaaggagacgagccttctcttgtgtgctgctctgtgctctgtgggttttttctacccctgctgatgggcctcatcccccagctctgcgtcatttacctctgaccctgtggaggagggagggtgcccaccctctcccctgccatggcacctcatgctagaggacacagtaccacctttgccccgttccccatctgctctggagctggtgggaagggcagacgcagttacctgggaagtcacaccctcagcaggtgtgtgtatccgtgtatgcatgtgggtgtcttactaatgacaatagtctcttcttgctgtgtctttagaaatcttttaaagtaacaggaccccccctcccccccagtctaagaagcactgggactgcctgctatcccattggcaagtggcaccttctcaaactgtttccaaaacgatagaaaaacactcagttggcctaacatggtcaggctagggaactggttaaactagtgaacttttagaatctgaaattggattgttttcaaccattcttagaaccgcgtgtttctaaggtgtgtcttcagccccttgccatgggatcttagtcatgcaagtgagttttttagaatgcagattctcacctgctgccagcttcttggatctggcactgtgttcgaggccacccctcaacccccaccgtggctctgtaggcgctttctgaggccgtccccccttatgatgccagggggacaggaaggaacccctatcagagctcacacctaggaacacttgtgggctggggcaaaaaatcttgagctgggagttttgttttgatcaccttgaaggataggttttctttctgatgggccaaagtactggaagcaaagtactttctgtagtagcccatgcttacttgtgacacttttaatatgaaatgtatccccagaccaaatgtccctaattgagttagatacacaccatttacatgtctgatggtgcagagctcataaatggctatagttccaatttctcaggtaaataattttccccaattcccgccctccccttattattaagattagtttcgagacagtgtgagacctcagagtgcggtagcagctccggccatcttctgcaggagccagctttttgtgtgtatctgtacccaagactggtgacaggtgccgttttctcctgaggtgatacctggccaagtttgacgactgcccacttgctagctgtgttcccaaccccactgttaagtaaggtgtgccttgctttaagcaaactgaatgtgattgtgttgtgctctgtatccatttggaaggaaaactaggatgtgcctgtctctttaagagaggctacccaattagcctttcatggtgggactctcctagtagtacactgtaagatactcactcttaaaaacccatcatgacatccatccctttaccactgtgtgcaccatgcagcaggtccctccctccccaagctgatgaagcaatgcaaaactctagagagacatcgaggccccattccatagatttagcccttagtgatactagcaaaactcccaccctgtcactgcgtgttttaagcccagattctcctagtccccatcaagtctgaaaccgagcacgcctgtacttctagtcaggggctgttgggttttggcttccttctcccttttggggaaatcctcacatgcacacttgataccactttgggtctgctctgccttctccagatttggcctcttggccactcctcacacccacccgcttgctggcctccaggccacagggcactgccagtgaggagactgctagctccgtgctgtctgcagctggcccaacatctgtatcctaatgtttacctgtcaccgagcggcgcttgatagaaagatggcccctgcagcgggcttgggcaggtttggtttttttggttttggttttgtttttccattgtcttgttcttgtgttattttattggctttaaatgcaaatgttgctatttattttttcctctctctcctgctctggtttgactaagctgatctggctatcccaatgcatgtggtacctgatagatggatatggtagagattatttcccctgccttttataactgtcccagtgtttaccttcaggcttgctaatgctttatttttggaagccacagatgtagtttttctggatgaattgcttttcctatagatgcagcggtatgcttttgcattgatctgcagggtgatttgaattttatttttacaagcagtttttcaatcaaattctatatttgatacaatgtgttaaggttagaccagcccgaagtcatgcagctggggacccctgagtgtccaggaggccttagggctgggtggcctctcttggtagtccctgtggtggtagcactggtcacaggaacctgcagggtccttcagcatggccctgaactcttggattgttacaaggattttttttttttcatgggcattttgctacacagacagtggacaatcccggtggccccagacaagccataagcaacctttctgtgacctccccctgactcatctctcacacccatgagaaaaggaatgtgccctgagtagaagctacacttccttggtgttcatctgcctcacttgtgaatgcagatattttgtttgttttaagcccttccctgcagtaaccatgctggctggctctcacataggcagagcacacgtgactcttgtcacaagcgccccgttgcaggcatgccgactcctgtaagttgcactgatctctcaacagtttcctttattgcgtccaactccccatctgctgtactcatttgaagtgattgtaaacagattcacagtggcaagagtgggacattgcctcacgtctgccatcccaacacacatttcagtattacatgctcgagtgtgcgggctgggctcttactctctcgagcattgtgggctagccttgtctcaacactctgacaagaagttctccctctctatacaggacagcatgctctttgagacctgaaggcttgaatgtaacagattagttaggagtctttgtggcgctttggtctgtttagttttcttgtgatctgcaaggccccccaaaagtatgtccttctagttccgtgtggcctttgtcagcatggacagcatatgaatagcccagtcagtgttaacaggccattgctacattctttggtcagcccctcaacttaaagttgtgaggtcactgctgtgtcagactgtcatttgtggtccatccatgggagacctaaagcctggtgcagccctttatgccatgtgaccttgctgggacctaagtacatggtctgtttagccccacgtggactgtctgtagtcagggtgcccagagtctgaaacaaaaggaattattacactcaaaccgcgggggaggggggtactggggacggacggacagacagacagacagacagacagacagacagacagtgccattgcagacattgcagagccctccctcaccttgtgtctttggatttgttcagccattttatttgtctcaagttgaatgtaccggcttagccaaatcactacagaaggtggtttctttaaaccttttagacagatgcataaaagctacctctgacttctcatagatttgtgtttgctctgaagcatcgctatccacactgaatatagccaaaatatcgcctgaataaaccgagttacgaactcctctgttcagttctggcttgaaaatgtgtgagtgccatactgtgatctggggccgcagctcctctgctgccactgacccagggactcctcagcaccacccagctgcaagatcctagcgtccgagaggctggtagactggcttctctgcatggggaacgtgcaccctccaggctggcgggcgtgactgcgcagggccgcgtgcggctgagcctggagatggtctgttcctgcctggctttgctttcctgttgaatcgtgtaagaaggcatctttacccctgcttctgaatggattgaaagtggctttcctgctctctcctccctttttgtacactataaactcataaaggtgcttcaacaccgactcttgtgcagataatgtagttaagcagttgcatggattcagatagtgcaggcacagctgaccttgggaccctggtgaaagtgaaggactgaaagccctgcgctccatttggaaggagcaatgggctctgcttgactagaaggtccctttagtgttggcagaacagagatttgtcatggggacctgccacctctgaccaagctgtcctgtggctgtctgctttgctgtccaggaggtcatcctcttacatcatggctccacctgcatctccttggcctctccacctaaagtttccttggaaagtcagcataagatcccagatttccctgtttagcttgagggacagatggacagtaccatgaagggacagattgacagtgtcgtgaaggtcggtgcctggggattgggctgggcttaggggagctcttctaaaggttgtctctgcgccctccccaacctcccccacatacacccccattgtattgttttggggggtgtcatcttccattcttttgacttggacagctcagtacccttaagagccctcacatatttctctgtgtagagaacctgaggcgtggagggtggtggggtgtctgcaacacccctgacctgcaccccaggttacttaaatcccctgacttggctggaatttgaaaatccagggaagcacagggtgctagatatgttacacttagggtcacacccaggtgccgtggtgtaagccacagccctcagggcaccagcacagaggcagccagcctccccactcgtgacatttgggaaggatactaaacaagccagagccctggccgctgcagtattcagctaacacttctttttcccggttgaaaggagaacactatttgaaaccttctaatgccctggtggttctagttcctccgttcctgagggctaaaaggttgaatgagctcctgttttgcttagagctgcctttatttgagtccatttaaaggtgaagcacgggactgtctcctctccctgagtcaccagcaagtaggtgcggagacccttctcattcagtcctttctgtatcttcaagcgccccaacatcagtgggatcacgagagcacccacatcagccatcagtggagtcaagtatacatatggggacagaaattggcaaatttacaggttgtaatggccactccagcctgtgacagggcaggccctccatcctgcacagcaggtggcctctgtcctacctccaggcactggctttactatctggacacctagttgagagccttgaaagtctgttcttggtgtccttttagaatcatgctggggctggtcgttagttgaccctgcccagaagctgtcagccctcagctctttcttgcaaactgtgatgccaccttgtagcctacggctgtggagccccgtcctgggttctttgtgccctgaaatgcaggtgcacctctgctgggggttggtggggcactggcaaaggctacttgctggccttgtgttctctgcccctcagcaagactgtctgttgtagtggaagtaggtttagagggatttggaatcatgtctttgtgaatcgagtgtgacgtcatcaggggtacggtgctacttggtcctaatgttttggaatgcttttccgtcgttgttactccattgttactctctgttctatgacactgagggagactgctcacttaggtgtggtgtcacataggactccagctttgatgtgctgtgcgggtggtgtgattatcatctctgaccttctgaagccaggggtggtacccatccctagctcagtctaattaaatctttcctccctgtcaagtgattgctttgggtggttttaacaggagtagtccagtctggatattctattttcttaattacatagaaaaggcccgtgcagcactagctgcagacagataacacatttaacaattttggttcctcttcaaagccaaatagacctatgttgattaaactccaagagggtacaattcagcattaaattaaaatttgagttgtgaatttgagagggaatttcagtatagttgataaagttaagtggcaagtgagtatttataaaagttataagagaataattgtaagtttctgtacaacaatttggctactgcctcataaatacaggaagagaccgtagccctgaatccgactctctcacctcccacataccttggactacctgcctaatatgtatttgagggggtttaacccttggttttatcaaattttctagttttattttatttttataaaataattgaaaaagaaccaaaaaggaatccagaagttccccagtggctcctcccccagtcttaatcccctcccccccacacacacacacacactcaaatgttttagaagacattttgaatcaaaatgtattccacccatttaacgtttgctactttctgagaagtggagtggctacgagggcctgtttcagatggtactctgtatgtcatggacatgtttgtacctgtacagtggatctgtgtgacgacgtcttgtgcttgcggtttagaaaccacgcagtgctgttttgtaaattgacagtttctgtgtaaactattatttatatcccttggcccctctgtataatttttcatttgctacatggattgtataattaattataaaattatagtacctgctaaacccaattctgaggcttcatgtcattgtctttggtttgataccagtctgatcgggaggctgcccaggccttgcgcaataccagtagtgatgttacttaaagttaagccttaatcagacagtgaatttgtgcatttcagagtatgcaaaaaaaaaaaaaaaaaaaaaagcagagtgagtttcaactgcccaggtcaagatgttcaaccctaactaacaggttccttctggagtgaaaaagtctaatgggttccctctggggtgaaaaaagggccgttacccgggttacactcttccctcccacctgcgctctcccatgtggcgtgatgaaactgatcgtgtgcatttctgtggcagtctatctagagacctttgcagggttgtcttcaaatgtctggtttctctcctctggtctgtgacgagcgagcgtctactgccgggagcacagctggggcgtaggtttcaccaacctgctcagcggccaagctctccgctgttactgccacctgctattgcttctgcatagattataacagatttgtgtcgggtaaaagcatcttcaatcttgaggtaaattagaggggacccagctttcttttacaatgtggatctacctcagttaaacagttgggtgctattaactaagtctgtcaaattaaattggaaagtaaccaaacagtgggacataactaactccacgtgaaacttgaagacatcatttccgtttctttaataatactttttatttattttgtgccttttatgttaaccctaatgcgttttttaaaattcagaatagtcatttttctcttgcgtgtggggagcccaactgaccatacaagcagccatctaactgctagagtatactgggatcctaacctaccctcttgggaaacggagggggaaaatgtgtccaagagcaaatttcacacatcacataccccagctcttacatgcttaccttcatagctttcgcttcatttctgtgccatctgaaggggcaacccccaaaactgacactcgagtgctttgtctctgctttttcagctgtccgtgtctctcttctctggcatgttttctataagactttctttcataaacagcaagtggactgttgtcgatcccatctcctggcttgcacagtgaaggtgctaaacgccaggagcagaggcgggggcaagctcacggttgtctggaggccaggccggaggcggggaggatgtgcacgatgcggcagcccgcggtttatgaagtcaggaacactctggtaagaaaataagatcggagatcgataactttccatgcctttatatttggcagtttacaacttttcctactacggcaacctttttgccataattctttggaggtaacacaatattttgaagtcttatgaatttgattttataccagtgagaagtcaaagttttagcggtctttcctgtagtgtatggttttactcttttaaagtcatctctggattttagaagagaacctattgtacttggtggctccaaaaggtgatttggggagccctgtcccttcccttcttgctctgctcccccccccccctttccctttgcagggtctcaaaaaaggaaaacaagaaaaaaaaatgatggttctagttagatttgtgggggttttttattgttggtttttttttttaagcttccatcaggttgatacttaagctatattttatagaataaatccccctttagtatgctgcatatggatatgaagctcagagctgttgcactttctctgtaatcaatgccctccaactggctcgctggaagcattttcttttaacctaatgtgtactttgggcaggtgatgaaatttacacaggtacctcaagtcaagacctgcgcgtgctgcatttggatttttggttttgttttgttttgcttttctatgtatcattatgttttacttaattttgttctggtgttatgctttttggaccccaatagtctgttgaaagaaaaacctaaaaagttagtatccttgttttctcccccctgcagtatgtggttctaaaggtcaccccccccccccattaggaatctcccccaagcacacccgaggaaaagggtacgacgggcgcttctcagcgggccgtgccattgagatcttttatattcatgctgcttctagtcccttctccacatcacctaagggtgggtacctccattgcgatgttgtggcatcttaacttttacacacagacctcatgggtgtccactgaggaagtagtatgagcctaaagacactggcagcgtgcagcctggcctgggagtggcggccttcgcatctctctctctctcagcactcagccacgctgacctggccgggtggtccgcgtggacaggacagctggtgaagcaagccggatgctcagaacactgcgcgtctcctgtcctgttttaagcttagctgggtcctttgttttcacagcttcctaggttaaatagtttgcactatttttgcaataaattcatgggaaacctaatggctacttatgttgttgttcttactgtgtgtataaactaacactaaaggtttgctatagtgttttcttttcacctccttatgtagccccataacatgcacagaatgctgtaaacctgattaagatatgatgtggatattttataaagttattttgtatggtgtcgattttgttttgccgcatagtgtgtcagcaaaaaataaaataaaataaaattcctcgtatttacagctgccta

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