LOCUS       NM_029475               2367 bp    mRNA    linear   ROD 20-NOV-2025
DEFINITION  Mus musculus adenosine deaminase-like (Adal), transcript variant 1,
            mRNA.
ACCESSION   NM_029475 XM_001003523 XM_485055 XM_900839 XM_900849 XM_900856
            XM_900859 XM_900862 XM_917792 XM_925262 XM_925265 XM_925269
            XM_925272 XM_925275 XM_986097 XM_986134 XM_986169 XM_986201
            XM_986236 XM_986298
VERSION     NM_029475.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2367)
  AUTHORS   Ogawa,A., Watanabe,S., Ozerova,I., Tsai,A.Y., Kuchitsu,Y.,
            Chong,H.B., Kawakami,T., Fuse,J., Han,W., Kudo,R., Naito,T.,
            Sato,K., Nakazawa,T., Saheki,Y., Hirayama,A., Stadler,P.F.,
            Arisawa,M., Araki,K., Bar-Peled,L., Taguchi,T., Sawa,S., Inaba,K.
            and Wei,F.Y.
  TITLE     Adenosine kinase and ADAL coordinate detoxification of modified
            adenosines to safeguard metabolism
  JOURNAL   Cell 188 (22), 6151-6169 (2025)
   PUBMED   40840445
REFERENCE   2  (bases 1 to 2367)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 2367)
  AUTHORS   Ingham,N.J., Pearson,S.A., Vancollie,V.E., Rook,V., Lewis,M.A.,
            Chen,J., Buniello,A., Martelletti,E., Preite,L., Lam,C.C.,
            Weiss,F.D., Powis,Z., Suwannarat,P., Lelliott,C.J., Dawson,S.J.,
            White,J.K. and Steel,K.P.
  TITLE     Mouse screen reveals multiple new genes underlying mouse and human
            hearing loss
  JOURNAL   PLoS Biol 17 (4), e3000194 (2019)
   PUBMED   30973865
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2367)
  AUTHORS   Lesscher,H.M.B., Bailey,A. and Vanderschuren,L.J.M.J.
  TITLE     Genetic Variability in Adenosine Deaminase-Like Contributes to
            Variation in Alcohol Preference in Mice
  JOURNAL   Alcohol Clin Exp Res 41 (7), 1271-1279 (2017)
   PUBMED   28449374
  REMARK    GeneRIF: genetically protective factor against alcohol consumption
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK048809.1, BC050879.1 and AK049697.1.
            
            On Mar 20, 2014 this sequence version replaced NM_029475.1.
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (a). The 5' UTR of this variant is incomplete due to lack of a
            complete transcript and splice pattern ambiguity in the upstream
            region.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC050879.1, SRR1660817.85134.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-48                AK048809.1         241-288
            49-2365             BC050879.1         1-2317
            2366-2367           AK049697.1         2467-2468
FEATURES             Location/Qualifiers
     source          1..2367
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 60.37 cM"
     gene            1..2367
                     /gene="Adal"
                     /gene_synonym="4930578F03Rik; Mapda"
                     /note="adenosine deaminase-like"
                     /db_xref="GeneID:75894"
                     /db_xref="MGI:MGI:1923144"
     exon            1..211
                     /gene="Adal"
                     /gene_synonym="4930578F03Rik; Mapda"
                     /inference="alignment:Splign:2.1.0"
     misc_feature    116..118
                     /gene="Adal"
                     /gene_synonym="4930578F03Rik; Mapda"
                     /note="upstream in-frame stop codon"
     CDS             152..1234
                     /gene="Adal"
                     /gene_synonym="4930578F03Rik; Mapda"
                     /EC_number="3.5.4.-"
                     /note="isoform a is encoded by transcript variant 1;
                     adenosine deaminase-like protein; N6-Methyl-AMP deaminase"
                     /codon_start=1
                     /product="N6-Methyl-AMP deaminase isoform a"
                     /protein_id="NP_083751.1"
                     /db_xref="CCDS:CCDS16633.1"
                     /db_xref="GeneID:75894"
                     /db_xref="MGI:MGI:1923144"
                     /translation="

MEAGQQWPGKTDFYLQLPKVELHAHLNGSISSSTMKKLIAKKPHLNVHGHMTMIDKGKKRTLQECFQMFQVIHQLTTSAEDILMVTKDVIKEFADDGVKYLELRSTPREENATGMTRKTYVESVLEGIKQCKQENLDIDVRYLMAIDRRGGPTIARETVELAKEFFLSTENTVLGLDLSGDPTIGQANDFLEPLLEAKKAGLKLALHLAEIPNREKENQMLLSLLPDRIGHGTFLSASEAGALDQVDFVRQHQIPLELCLTSNIKSQTVPSYDQHHFGFWYSIAHPSVICTDDKGVFATYLSQEYQLAAETFNLTPFQVWDLSYESINYIFACDNTRSELRKRWTHLKQKVLNCNEVNYF"

     misc_feature    200..1183
                     /gene="Adal"
                     /gene_synonym="4930578F03Rik; Mapda"
                     /note="Adenosine/AMP deaminase. Adenosine deaminases
                     (ADAs) are present in pro- and eukaryotic organisms and
                     catalyze the zinc dependent irreversible deamination of
                     adenosine nucleosides to inosine nucleosides and ammonia.
                     The eukaryotic AMP deaminase...; Region: ADA_AMPD;
                     cd00443"
                     /db_xref="CDD:238250"
     misc_feature    order(218..220,224..226,770..772,779..781,842..844,
                     1025..1030)
                     /gene="Adal"
                     /gene_synonym="4930578F03Rik; Mapda"
                     /note="active site"
                     /db_xref="CDD:238250"
     misc_feature    842..844
                     /gene="Adal"
                     /gene_synonym="4930578F03Rik; Mapda"
                     /note="Important for catalytic activity.
                     /evidence=ECO:0000250|UniProtKB:P03958; propagated from
                     UniProtKB/Swiss-Prot (Q80SY6.1); other site"
     exon            212..342
                     /gene="Adal"
                     /gene_synonym="4930578F03Rik; Mapda"
                     /inference="alignment:Splign:2.1.0"
     exon            343..403
                     /gene="Adal"
                     /gene_synonym="4930578F03Rik; Mapda"
                     /inference="alignment:Splign:2.1.0"
     exon            404..491
                     /gene="Adal"
                     /gene_synonym="4930578F03Rik; Mapda"
                     /inference="alignment:Splign:2.1.0"
     exon            492..573
                     /gene="Adal"
                     /gene_synonym="4930578F03Rik; Mapda"
                     /inference="alignment:Splign:2.1.0"
     exon            574..700
                     /gene="Adal"
                     /gene_synonym="4930578F03Rik; Mapda"
                     /inference="alignment:Splign:2.1.0"
     exon            701..781
                     /gene="Adal"
                     /gene_synonym="4930578F03Rik; Mapda"
                     /inference="alignment:Splign:2.1.0"
     exon            782..920
                     /gene="Adal"
                     /gene_synonym="4930578F03Rik; Mapda"
                     /inference="alignment:Splign:2.1.0"
     exon            921..1021
                     /gene="Adal"
                     /gene_synonym="4930578F03Rik; Mapda"
                     /inference="alignment:Splign:2.1.0"
     exon            1022..2367
                     /gene="Adal"
                     /gene_synonym="4930578F03Rik; Mapda"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2344..2349
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Adal"
                     /gene_synonym="4930578F03Rik; Mapda"
                     /note="hexamer: ATTAAA"
     polyA_site      2365
                     /gene="Adal"
                     /gene_synonym="4930578F03Rik; Mapda"
                     /note="major polyA site"
ORIGIN      

gtgaaagtgatcccagagccacacatagtgcaagtcttgtttaaaatggcaatctgaaggaagtccagcacctcagagattcccactttgataagtttatcgtccaagccgagaataagagcaaagagaaacttttccttctactgaagtgatggaggccggacagcagtggcctgggaagacagacttttatctgcaattaccaaaagtggagctccatgcccacttgaatggctccattagttccagtaccatgaagaaactgatagccaagaagccacatcttaacgttcatggtcacatgaccatgattgacaaggggaagaaaaggactttacaagaatgtttccagatgttccaagttatacatcagcttactactagtgctgaggacatcctgatggtcacaaaagacgtcattaaggaatttgcagatgatggtgtcaagtacctggagctgaggagcactcccagagaagaaaatgctactggaatgactaggaagacttacgtggaatccgtccttgaaggcataaaacaatgcaaacaggaaaacttagatattgatgttaggtatttgatggcaattgacagaagaggtggcccaacaatagccagggagactgtcgaacttgctaaagagttcttcctctctactgagaatacagttcttggccttgacctcagtggagatccaactataggacaagctaacgacttcttggagcctcttttagaagctaaaaaagcaggtctgaagttggcattgcatcttgccgagattccaaacagggaaaaagaaaatcagatgctgctgagtctgctccctgacagaattgggcatgggacattccttagcgcctctgaggcaggggccctggaccaagtggacttcgtgaggcaacaccagatacccctcgaactttgtttgacctcaaacatcaaaagccagacagtcccttcttatgatcagcatcactttggattttggtacagcattgctcatccttctgtgatctgtactgatgataaaggtgtttttgcgacatacctttctcaagaatatcaactggcagcagaaacatttaatttgaccccatttcaggtgtgggatctgtcctatgaatccatcaactacatctttgcttgtgacaacaccagatctgaactgagaaaaaggtggactcacttgaagcagaaagtgttaaactgtaatgaggtgaactacttttgattatacattgtagccaagatcttcctgccctgtgtatcctactcagttactgcccacttgaaatgacttcccttgaatttgggtagccaagtatatatggggtctaactccagccccttcaactggtaagttcgaggttttgcatggctgcacttccatgctgcttttgctggagggttgtttagccctcatttcctagtgactcttgtttttacacttagtgctctttagacatttgtgaccgtgaaccttctgagaagttgaaactctttgtcttcagggaaatctctgttttgttaggtttaatcagactccttccacttccttttaccctttcaagtattaacttttttagactgttgatgttaggtcacagtgcacacctgaactgtttgtgtgggtgcctagttgtcaggtgactgtagtaacaaagctagaatctggctttgcccttctcctaatatctttagaaatatataacgggggttggagtaatcccttcagtttaagaaaagaaaagaacgactggcttgaagtaaaaaagacacatagctggagcttctgatttaaaatcaggttctatttggcttccttgtggaagctaccaggacctctacagtgctaggactctcttaaaagatctttgtagcaatggtagcattgtggcttctagggttcttagatcaccaataatcccatgcccacatgatagcctacaggtatagacttatataaacacacatataccatggccacattagccgactttagcagataacaaatgtcttggttggtttttttcacaagctagagtcatttgggaagagggaccctcaactgagaaaatattcccacaagattggcccaatcagtgcttgatcagttagtgggcccatgccacagagggtgataccacccttagggacatggttctgggtggaaagcaaaggaaatggaacaaggcacgaggagcaagccataggcaacactcctccatggcttcagctttagttcctgcctcaacttcccaggatgacagactacaaattataagaggaaataaccctttcctccttacattacatttggttatggtgttttgtcacagcattaaaaacactaaaacaataatg

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