LOCUS       NM_026626               3665 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus EF-hand calcium binding domain 2 (Efcab2), mRNA.
ACCESSION   NM_026626
VERSION     NM_026626.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3665)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 3665)
  AUTHORS   Shawki,H.H., Ishikawa-Yamauchi,Y., Kawashima,A., Katoh,Y.,
            Matsuda,M., Al-Soudy,A.S., Minisy,F.M., Kuno,A., Gulibaikelamu,X.,
            Hirokawa,T., Takahashi,S. and Oishi,H.
  TITLE     EFCAB2 is a novel calcium-binding protein in mouse testis and sperm
  JOURNAL   PLoS One 14 (4), e0214687 (2019)
   PUBMED   30933994
  REMARK    GeneRIF: EFCAB2 is a novel calcium-binding protein in mouse testis
            and sperm.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3665)
  AUTHORS   Lewandowski,J.P., Du,F., Zhang,S., Powell,M.B., Falkenstein,K.N.,
            Ji,H. and Vokes,S.A.
  TITLE     Spatiotemporal regulation of GLI target genes in the mammalian limb
            bud
  JOURNAL   Dev Biol 406 (1), 92-103 (2015)
   PUBMED   26238476
REFERENCE   4  (bases 1 to 3665)
  AUTHORS   Koscielny,G., Yaikhom,G., Iyer,V., Meehan,T.F., Morgan,H.,
            Atienza-Herrero,J., Blake,A., Chen,C.K., Easty,R., Di Fenza,A.,
            Fiegel,T., Grifiths,M., Horne,A., Karp,N.A., Kurbatova,N.,
            Mason,J.C., Matthews,P., Oakley,D.J., Qazi,A., Regnart,J.,
            Retha,A., Santos,L.A., Sneddon,D.J., Warren,J., Westerberg,H.,
            Wilson,R.J., Melvin,D.G., Smedley,D., Brown,S.D., Flicek,P.,
            Skarnes,W.C., Mallon,A.M. and Parkinson,H.
  TITLE     The International Mouse Phenotyping Consortium Web Portal, a
            unified point of access for knockout mice and related phenotyping
            data
  JOURNAL   Nucleic Acids Res 42 (Database issue), D802-D809 (2014)
   PUBMED   24194600
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC157809.4.
            
            On Apr 11, 2018 this sequence version replaced NM_026626.2.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK029880.1, BC025062.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849380, SAMN00849381
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-116               AC157809.4         30463-30578
            117-201             AC157809.4         61847-61931
            202-274             AC157809.4         80261-80333
            275-354             AC157809.4         99327-99406
            355-397             AC157809.4         100130-100172
            398-464             AC157809.4         100270-100336
            465-3665            AC157809.4         105758-108958
FEATURES             Location/Qualifiers
     source          1..3665
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="1"
                     /map="1 83.16 cM"
     gene            1..3665
                     /gene="Efcab2"
                     /gene_synonym="1700073K01Rik; D830011E08Rik"
                     /note="EF-hand calcium binding domain 2"
                     /db_xref="GeneID:68226"
                     /db_xref="MGI:MGI:1915476"
     exon            1..116
                     /gene="Efcab2"
                     /gene_synonym="1700073K01Rik; D830011E08Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             86..580
                     /gene="Efcab2"
                     /gene_synonym="1700073K01Rik; D830011E08Rik"
                     /note="EF-hand calcium-binding domain-containing protein
                     2"
                     /codon_start=1
                     /product="dynein regulatory complex protein 8"
                     /protein_id="NP_080902.1"
                     /db_xref="CCDS:CCDS15557.1"
                     /db_xref="GeneID:68226"
                     /db_xref="MGI:MGI:1915476"
                     /translation="

MAEERDAEGTEALIAELHKKIKDAFEVFDHESNNTVDVREIGTIIRSLGCCPTEGELHDFIAEIEEEEPTGYIRFEKFIPVMTRALVERRYRPAAEDILLRAFEVLDPAKRGFLTKDELVKYMTEEGEPFSQEEMEEMLSAAIDPESNTINYRDYITMMVVDEN"

     misc_feature    113..565
                     /gene="Efcab2"
                     /gene_synonym="1700073K01Rik; D830011E08Rik"
                     /note="calmodulin; Provisional; Region: PTZ00184"
                     /db_xref="CDD:185504"
     exon            117..201
                     /gene="Efcab2"
                     /gene_synonym="1700073K01Rik; D830011E08Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            202..274
                     /gene="Efcab2"
                     /gene_synonym="1700073K01Rik; D830011E08Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            275..354
                     /gene="Efcab2"
                     /gene_synonym="1700073K01Rik; D830011E08Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            355..397
                     /gene="Efcab2"
                     /gene_synonym="1700073K01Rik; D830011E08Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            398..464
                     /gene="Efcab2"
                     /gene_synonym="1700073K01Rik; D830011E08Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            465..3665
                     /gene="Efcab2"
                     /gene_synonym="1700073K01Rik; D830011E08Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      576..581
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Efcab2"
                     /gene_synonym="1700073K01Rik; D830011E08Rik"
                     /note="hexamer: ATTAAA"
     polyA_site      612
                     /gene="Efcab2"
                     /gene_synonym="1700073K01Rik; D830011E08Rik"
                     /note="major polyA site"
     polyA_site      668
                     /gene="Efcab2"
                     /gene_synonym="1700073K01Rik; D830011E08Rik"
                     /note="major polyA site"
     regulatory      764..769
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Efcab2"
                     /gene_synonym="1700073K01Rik; D830011E08Rik"
                     /note="hexamer: CATAAA"
     polyA_site      788
                     /gene="Efcab2"
                     /gene_synonym="1700073K01Rik; D830011E08Rik"
                     /note="major polyA site"
     regulatory      1126..1131
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Efcab2"
                     /gene_synonym="1700073K01Rik; D830011E08Rik"
                     /note="hexamer: AGTAAA"
     polyA_site      1150
                     /gene="Efcab2"
                     /gene_synonym="1700073K01Rik; D830011E08Rik"
     regulatory      3643..3648
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Efcab2"
                     /gene_synonym="1700073K01Rik; D830011E08Rik"
                     /note="hexamer: AACAAA"
     polyA_site      3665
                     /gene="Efcab2"
                     /gene_synonym="1700073K01Rik; D830011E08Rik"
                     /note="major polyA site"
ORIGIN      

accgtgcatccaggggctttctccgcccgcggggtcccgcagggttcgtgctggatgtctcccgggcgaccggctccgcaccaagatggcggaggaaagggacgcggaagggacagaggcactaattgcagaacttcataagaagatcaaagacgcatttgaagtgttcgaccatgaatcgaataatacagtagatgtgagagagattgggacaattatcaggtcattaggatgctgcccgactgaaggagagctgcatgatttcattgcagagatagaggaagaagaacccactggctacattcgatttgaaaaatttattccagtgatgaccagagcccttgtagaaagaagatacagaccagctgctgaagatatccttttgcgagctttcgaggttttggatccagctaaacgcgggtttctcactaaggatgaacttgtcaagtacatgactgaggaaggtgagcctttttcccaagaggaaatggaagaaatgttgtctgctgcaattgatcccgaatcaaacaccattaattatagggactacataacaatgatggtggtagatgaaaattaaatgctgcaaagacttcttttttctccttttaagacaactgtaaacattcaatttctagttgaaatgataatttgaaaatttgtcctaattaatttcacattttattgtataatttctacatttagtgccttatgacataattgtttcaaggtattttgtttactagagatgatcagtttaaaacataaatttatttagtatgaccagatttatggaaggacaagttaattatttcaaaggaattgtacagtattttaatgttaaatgtatcgttgcttattttccacaactaggccaatgatacattgaaactaattagaaagtgagattgatcctaaccccctaacatttgccatagaaagctcttgcagggcaggatggccacagactagatgtgagtggcagcttggtgatggctgcctttcagagcagcagtaaacacttcagcaagcagaatatggagggagagcgaccagaataactattaataattgatttcttatgaatgccaattacaaatctcatttgagagtctaataactttgagtaaaattgaaaattgctcatccagcattttgccatttatggaatagctaaaggaggtgagataattggatccagagaaaggaagactgaatgaaagctgataatggcaacttctttttttgctctttagaattctcacctattcagaatatctgtcatgggaatattgtcctggatactgcgtgtattcatctgttgttaccagaatgtagtatctgataagggctgactccagaagaaagagattcatttagctcacagttatagatggtcaaggtccaggcagcattgtacaggctgagctagggccacccgtgtatgcatctcaccaaggtacagctagccagacagaagcacaagcaagcaagagcagagaggctgggaggaggggtcaggcctgttccttttacaatagtcctctcataagaattactttccaggctggggagatggctcaatgggtaaagcatttgctgagtatcattagataatgtggtggaagctggagatgaaagatggataaacctctaattaatcaaggtcacagtttagtgagggagtcttgcaagtaagtcaatgagcaaccacacattcaggggactgaaggttgcacaaaagaagcaaaagaagcctggtgggatgagggtgctttctggtgggcagactcaaagaggtaacacatgacttacaaactcagtagttcttaagttttgacaaatagtcattctttgagcagtttacttcagagcatgtcagcagcaggacagcagatcaaacccccagaggtgtcctgaaacgcaagaatctgggattgaaagtatggttgatagatatttggaacctgatgagagatgagaggttgaaataagaaccatatcctaagagataatgagctaattggggttaaggtttcagtgtttgaacgtgaactttctctgagaatcagagagaaaggagctggagttgtaaatagcctgagaaatactttgaagggtcaaggccagagggaaggagaccagctggtaggctgcttcgttggcagtgagagatgggctagggaaagtcaagcacaaatcctaagtgtctggcttgaacacttggtaaataataatatcagccattcagataagatgcccaaggcttaggctaataggcttgactgtggtcatctccagcttgaagagctggtagaagataaaaagatcaagtggataaataaggggacatctgctagagacacaggctttgaagttgttagcacacggtgttctggaaagaaaataaaagatgaccaggacagaactgggagtacagaagaggacccctcagagcatggggagaagccctgaaaggaggaaaagctgaagaaagagaatccatggcagtagagtctcttaaggaagaaggaaccaagtcagaagtggtagagttgtgaggtcgctgatgatgaggaaatccctcagtttcagcaccaacacatccactgagaacctctctcagggcagatttgtgaagtggcaggcaggagagttagtgacttcaggaataaacagatgtgaggaagagcctggaggacacgtgtcgagtggtgaagagagagaaggtagaggggaggggtttggaagatgtaaacggtgcaagaatgctgcaagcttgtatttcaagagttgagacatggcagaggagcagaggacactcagaggggcagagatccagggctacaaaggtcttgaccttggtcatggacatgaactaatgtatatagtccctgcctagtggcttctagctcttataacgtgtgacctctctgcttctgcctccctagtgctaggattctaggcctgaacctctgcacccagttatactgtctctatgaatgagaaggaagatagtaggttatagagctagaggagatgatagaaatctgaagggctttggggctttgtgagaaatgagagagttggggaataatctacaaaagaattttcaaggattttgtccagtaacactggcccagaagcaaaatatgtagagtgttgtagtgagtcaagatggtgaaatgaacagacaaaaaaaaaaaaaaaaaaaaaaaagatcaagattgatgagtcagcagaaatgagtgaccttgatggtctgtaggcaaaggtgggagggaaatccagctaggagtagacttgcatggaagattgtaagactccaaagggcaaacagtgtgtggtgagaaggcctgtcattcctgagaacaatatgctctgcttgcagtttgacttctctgtactattactgagcatttctctgtgttattattaattagtaatagtatacagtgtatatacatctctcactatctgtcaggcactgttaagtgttttacttaacctactcatttaaaattcctgatgacccagtgaagtagttaccattactatgagggagtggcaaagaagttttgtaactataagggactgcattaatcagcctttgtaggctttgtgatgctgaatcttcaccacaacaaaactaatttttcatgtca

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