LOCUS       NM_001355748            2436 bp    mRNA    linear   ROD 20-NOV-2025
DEFINITION  Mus musculus adenosine deaminase-like (Adal), transcript variant 4,
            mRNA.
ACCESSION   NM_001355748 XM_006500358
VERSION     NM_001355748.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2436)
  AUTHORS   Ogawa,A., Watanabe,S., Ozerova,I., Tsai,A.Y., Kuchitsu,Y.,
            Chong,H.B., Kawakami,T., Fuse,J., Han,W., Kudo,R., Naito,T.,
            Sato,K., Nakazawa,T., Saheki,Y., Hirayama,A., Stadler,P.F.,
            Arisawa,M., Araki,K., Bar-Peled,L., Taguchi,T., Sawa,S., Inaba,K.
            and Wei,F.Y.
  TITLE     Adenosine kinase and ADAL coordinate detoxification of modified
            adenosines to safeguard metabolism
  JOURNAL   Cell 188 (22), 6151-6169 (2025)
   PUBMED   40840445
REFERENCE   2  (bases 1 to 2436)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 2436)
  AUTHORS   Ingham,N.J., Pearson,S.A., Vancollie,V.E., Rook,V., Lewis,M.A.,
            Chen,J., Buniello,A., Martelletti,E., Preite,L., Lam,C.C.,
            Weiss,F.D., Powis,Z., Suwannarat,P., Lelliott,C.J., Dawson,S.J.,
            White,J.K. and Steel,K.P.
  TITLE     Mouse screen reveals multiple new genes underlying mouse and human
            hearing loss
  JOURNAL   PLoS Biol 17 (4), e3000194 (2019)
   PUBMED   30973865
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2436)
  AUTHORS   Lesscher,H.M.B., Bailey,A. and Vanderschuren,L.J.M.J.
  TITLE     Genetic Variability in Adenosine Deaminase-Like Contributes to
            Variation in Alcohol Preference in Mice
  JOURNAL   Alcohol Clin Exp Res 41 (7), 1271-1279 (2017)
   PUBMED   28449374
  REMARK    GeneRIF: genetically protective factor against alcohol consumption
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL845479.8.
            
            On Sep 26, 2017 this sequence version replaced XM_006500358.2.
            
            Transcript Variant: This variant (4) lacks two alternate exons
            compared to variant 1. The resulting isoform (4) is shorter at the
            N-terminus compared to isoform 1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR13422600.598647.1,
                                           SRR1660817.99608.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849385, SAMN00849386
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-239               AL845479.8         52470-52708
            240-450             AL845479.8         54135-54345
            451-581             AL845479.8         54566-54696
            582-642             AL845479.8         55191-55251
            643-769             AL845479.8         62262-62388
            770-850             AL845479.8         63173-63253
            851-989             AL845479.8         64472-64610
            990-1090            AL845479.8         66761-66861
            1091-2436           AL845479.8         67377-68722
FEATURES             Location/Qualifiers
     source          1..2436
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 60.37 cM"
     gene            1..2436
                     /gene="Adal"
                     /gene_synonym="4930578F03Rik; Mapda"
                     /note="adenosine deaminase-like"
                     /db_xref="GeneID:75894"
                     /db_xref="MGI:MGI:1923144"
     exon            1..239
                     /gene="Adal"
                     /gene_synonym="4930578F03Rik; Mapda"
                     /inference="alignment:Splign:2.1.0"
     exon            240..450
                     /gene="Adal"
                     /gene_synonym="4930578F03Rik; Mapda"
                     /inference="alignment:Splign:2.1.0"
     exon            451..581
                     /gene="Adal"
                     /gene_synonym="4930578F03Rik; Mapda"
                     /inference="alignment:Splign:2.1.0"
     exon            582..642
                     /gene="Adal"
                     /gene_synonym="4930578F03Rik; Mapda"
                     /inference="alignment:Splign:2.1.0"
     misc_feature    638..640
                     /gene="Adal"
                     /gene_synonym="4930578F03Rik; Mapda"
                     /note="upstream in-frame stop codon"
     exon            643..769
                     /gene="Adal"
                     /gene_synonym="4930578F03Rik; Mapda"
                     /inference="alignment:Splign:2.1.0"
     CDS             650..1303
                     /gene="Adal"
                     /gene_synonym="4930578F03Rik; Mapda"
                     /EC_number="3.5.4.-"
                     /note="isoform 4 is encoded by transcript variant 4;
                     adenosine deaminase-like protein; N6-Methyl-AMP deaminase"
                     /codon_start=1
                     /product="N6-Methyl-AMP deaminase isoform 4"
                     /protein_id="NP_001342677.1"
                     /db_xref="CCDS:CCDS89543.1"
                     /db_xref="GeneID:75894"
                     /db_xref="MGI:MGI:1923144"
                     /translation="

MAIDRRGGPTIARETVELAKEFFLSTENTVLGLDLSGDPTIGQANDFLEPLLEAKKAGLKLALHLAEIPNREKENQMLLSLLPDRIGHGTFLSASEAGALDQVDFVRQHQIPLELCLTSNIKSQTVPSYDQHHFGFWYSIAHPSVICTDDKGVFATYLSQEYQLAAETFNLTPFQVWDLSYESINYIFACDNTRSELRKRWTHLKQKVLNCNEVNYF"

     misc_feature    <656..1252
                     /gene="Adal"
                     /gene_synonym="4930578F03Rik; Mapda"
                     /note="Superfamily of metallo-dependent hydrolases (also
                     called amidohydrolase superfamily) is a large group of
                     proteins that show conservation in their 3-dimensional
                     fold (TIM barrel) and in details of their active site. The
                     vast majority of the members have a...; Region:
                     metallo-dependent_hydrolases; cl00281"
                     /db_xref="CDD:469705"
     exon            770..850
                     /gene="Adal"
                     /gene_synonym="4930578F03Rik; Mapda"
                     /inference="alignment:Splign:2.1.0"
     exon            851..989
                     /gene="Adal"
                     /gene_synonym="4930578F03Rik; Mapda"
                     /inference="alignment:Splign:2.1.0"
     exon            990..1090
                     /gene="Adal"
                     /gene_synonym="4930578F03Rik; Mapda"
                     /inference="alignment:Splign:2.1.0"
     exon            1091..2436
                     /gene="Adal"
                     /gene_synonym="4930578F03Rik; Mapda"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2413..2418
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Adal"
                     /gene_synonym="4930578F03Rik; Mapda"
                     /note="hexamer: ATTAAA"
     polyA_site      2434
                     /gene="Adal"
                     /gene_synonym="4930578F03Rik; Mapda"
                     /note="major polyA site"
ORIGIN      

acgtaatagccgcggtggataagcggcgtgcgccgcacgggtcccgggaccagcagcgccgcaaaggggtcgcgcacgtatccgtgtgcggcaagcgatcgtttgctgagggagctgctgtcgttggtgctctggaccgttcccgcccggcgctggcggcctcgcgggcccatgacgagaaggtgctcagcaatactgcacacagaacacttaccgagctcccgctcatccgcttctgggtgaaagtgatcccagagccacacatagtgcaagtcttgtttaaaatggcaatctgaaggaagtccagcacctcagagattcccactttgataagtttatcgtccaagccgagaataagagcaaagagaaacttttccttctactgaagtgatggaggccggacagcagtggcctgggaagacagacttttatctgcaattaccaaaagtggagctccatgcccacttgaatggctccattagttccagtaccatgaagaaactgatagccaagaagccacatcttaacgttcatggtcacatgaccatgattgacaaggggaagaaaaggactttacaagaatgtttccagatgttccaagttatacatcagcttactactagtgctgaggacatcctgatggtatttgatggcaattgacagaagaggtggcccaacaatagccagggagactgtcgaacttgctaaagagttcttcctctctactgagaatacagttcttggccttgacctcagtggagatccaactataggacaagctaacgacttcttggagcctcttttagaagctaaaaaagcaggtctgaagttggcattgcatcttgccgagattccaaacagggaaaaagaaaatcagatgctgctgagtctgctccctgacagaattgggcatgggacattccttagcgcctctgaggcaggggccctggaccaagtggacttcgtgaggcaacaccagatacccctcgaactttgtttgacctcaaacatcaaaagccagacagtcccttcttatgatcagcatcactttggattttggtacagcattgctcatccttctgtgatctgtactgatgataaaggtgtttttgcgacatacctttctcaagaatatcaactggcagcagaaacatttaatttgaccccatttcaggtgtgggatctgtcctatgaatccatcaactacatctttgcttgtgacaacaccagatctgaactgagaaaaaggtggactcacttgaagcagaaagtgttaaactgtaatgaggtgaactacttttgattatacattgtagccaagatcttcctgccctgtgtatcctactcagttactgcccacttgaaatgacttcccttgaatttgggtagccaagtatatatggggtctaactccagccccttcaactggtaagttcgaggttttgcatggctgcacttccatgctgcttttgctggagggttgtttagccctcatttcctagtgactcttgtttttacacttagtgctctttagacatttgtgaccgtgaaccttctgagaagttgaaactctttgtcttcagggaaatctctgttttgttaggtttaatcagactccttccacttccttttaccctttcaagtattaacttttttagactgttgatgttaggtcacagtgcacacctgaactgtttgtgtgggtgcctagttgtcaggtgactgtagtaacaaagctagaatctggctttgcccttctcctaatatctttagaaatatataacgggggttggagtaatcccttcagtttaagaaaagaaaagaacgactggcttgaagtaaaaaagacacatagctggagcttctgatttaaaatcaggttctatttggcttccttgtggaagctaccaggacctctacagtgctaggactctcttaaaagatctttgtagcaatggtagcattgtggcttctagggttcttagatcaccaataatcccatgcccacatgatagcctacaggtatagacttatataaacacacatataccatggccacattagccgactttagcagataacaaatgtcttggttggtttttttcacaagctagagtcatttgggaagagggaccctcaactgagaaaatattcccacaagattggcccaatcagtgcttgatcagttagtgggcccatgccacagagggtgataccacccttagggacatggttctgggtggaaagcaaaggaaatggaacaaggcacgaggagcaagccataggcaacactcctccatggcttcagctttagttcctgcctcaacttcccaggatgacagactacaaattataagaggaaataaccctttcctccttacattacatttggttatggtgttttgtcacagcattaaaaacactaaaacaataatg

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