LOCUS       NM_001347463            2695 bp    mRNA    linear   ROD 23-JUN-2025
DEFINITION  Mus musculus 5'-nucleotidase domain containing 1 (Nt5dc1),
            transcript variant 2, mRNA.
ACCESSION   NM_001347463 XM_017313964
VERSION     NM_001347463.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2695)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2695)
  AUTHORS   Bitto,E., Bingman,C.A., Wesenberg,G.E., McCoy,J.G. and
            Phillips,G.N. Jr.
  TITLE     Structure of pyrimidine 5'-nucleotidase type 1. Insight into
            mechanism of action and inhibition during lead poisoning
  JOURNAL   J Biol Chem 281 (29), 20521-20529 (2006)
   PUBMED   16672222
  REMARK    GeneRIF: Inhibited by lead which binds at the active site and my
            inhibit lead poisoning.
REFERENCE   3  (bases 1 to 2695)
  AUTHORS   Piao,Y., Ko,N.T., Lim,M.K. and Ko,M.S.
  TITLE     Construction of long-transcript enriched cDNA libraries from
            submicrogram amounts of total RNAs by a universal PCR amplification
            method
  JOURNAL   Genome Res 11 (9), 1553-1558 (2001)
   PUBMED   11544199
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BB618670.1, AK049656.1 and BC098476.1.
            
            On Nov 29, 2016 this sequence version replaced XM_017313964.1.
            
            Transcript Variant: This variant (2) lacks an exon in the 5' coding
            region compared to variant 1. This variant represents translation
            initiation at a downstream start codon compared to variant 1; the
            5'-most initiation codon, as used in variant 1, is associated with
            a truncated ORF that would render the transcript a candidate for
            nonsense-mediated decay (NMD). Leaky scanning results in
            translation initiation at the downstream start codon to encode an
            isoform (2) that has a shorter N-terminus, compared to isoform 1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK049656.1, SRR7345562.1068740.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-23                BB618670.1         3-25
            24-2001             AK049656.1         1-1978
            2002-2695           BC098476.1         2024-2717
FEATURES             Location/Qualifiers
     source          1..2695
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="10"
                     /map="10 18.85 cM"
     gene            1..2695
                     /gene="Nt5dc1"
                     /gene_synonym="6030401B09Rik; Nt5c2l1"
                     /note="5'-nucleotidase domain containing 1"
                     /db_xref="GeneID:319638"
                     /db_xref="MGI:MGI:2442446"
     exon            1..191
                     /gene="Nt5dc1"
                     /gene_synonym="6030401B09Rik; Nt5c2l1"
                     /inference="alignment:Splign:2.1.0"
     exon            192..283
                     /gene="Nt5dc1"
                     /gene_synonym="6030401B09Rik; Nt5c2l1"
                     /inference="alignment:Splign:2.1.0"
     exon            284..355
                     /gene="Nt5dc1"
                     /gene_synonym="6030401B09Rik; Nt5c2l1"
                     /inference="alignment:Splign:2.1.0"
     exon            356..492
                     /gene="Nt5dc1"
                     /gene_synonym="6030401B09Rik; Nt5c2l1"
                     /inference="alignment:Splign:2.1.0"
     exon            493..572
                     /gene="Nt5dc1"
                     /gene_synonym="6030401B09Rik; Nt5c2l1"
                     /inference="alignment:Splign:2.1.0"
     misc_feature    521..523
                     /gene="Nt5dc1"
                     /gene_synonym="6030401B09Rik; Nt5c2l1"
                     /note="upstream in-frame stop codon"
     exon            573..747
                     /gene="Nt5dc1"
                     /gene_synonym="6030401B09Rik; Nt5c2l1"
                     /inference="alignment:Splign:2.1.0"
     CDS             689..1417
                     /gene="Nt5dc1"
                     /gene_synonym="6030401B09Rik; Nt5c2l1"
                     /note="isoform 2 is encoded by transcript variant 2;
                     5'-nucleotidase, cytosolic II-like 1; 5'-nucleotidase
                     domain-containing protein 1; cytosolic 5'-nucleotidase
                     II-like protein 1"
                     /codon_start=1
                     /product="5'-nucleotidase domain-containing protein 1
                     isoform 2"
                     /protein_id="NP_001334392.1"
                     /db_xref="GeneID:319638"
                     /db_xref="MGI:MGI:2442446"
                     /translation="

MLITSSHSDYCKLLGSYILGEDFADLFDIVITNALKPGFFSHFPSQRPFYTLENDEEKDELPSLDKPGWYSQGNAAHLYELLKKMTSKPEPKVVYFGDSMHSDIFPAHHYTNWETVLILEELQGPEMEKPEEAEPLEKRGKYEAPMVKPLNTLSNKWGSYFIDSVSGRGRAEDSVVYTWSSKRISTYSTIAIPNIESIAELPLDYKFTRFSTNNSKMAGYYPLVHHTLGSQDTDSKIISTEK"

     misc_feature    <692..1054
                     /gene="Nt5dc1"
                     /gene_synonym="6030401B09Rik; Nt5c2l1"
                     /note="The haloacid dehalogenase (HAD) superfamily
                     includes carbon and phosphorus hydrolases such as
                     2-haloalkonoate dehalogenase, epoxide hydrolase,
                     phosphoserine phosphatase, phosphomannomutase,
                     phosphoglycolate phosphatase, P-type ATPase, among others.
                     These...; Region: Haloacid Dehalogenase-like Hydrolases;
                     cl21460"
                     /db_xref="CDD:473868"
     exon            748..845
                     /gene="Nt5dc1"
                     /gene_synonym="6030401B09Rik; Nt5c2l1"
                     /inference="alignment:Splign:2.1.0"
     exon            846..964
                     /gene="Nt5dc1"
                     /gene_synonym="6030401B09Rik; Nt5c2l1"
                     /inference="alignment:Splign:2.1.0"
     exon            965..1117
                     /gene="Nt5dc1"
                     /gene_synonym="6030401B09Rik; Nt5c2l1"
                     /inference="alignment:Splign:2.1.0"
     exon            1118..1286
                     /gene="Nt5dc1"
                     /gene_synonym="6030401B09Rik; Nt5c2l1"
                     /inference="alignment:Splign:2.1.0"
     exon            1287..2657
                     /gene="Nt5dc1"
                     /gene_synonym="6030401B09Rik; Nt5c2l1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2636..2641
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Nt5dc1"
                     /gene_synonym="6030401B09Rik; Nt5c2l1"
                     /note="hexamer: AATAAA"
     polyA_site      2657
                     /gene="Nt5dc1"
                     /gene_synonym="6030401B09Rik; Nt5c2l1"
                     /note="major polyA site"
ORIGIN      

gctccgcgtcctgcctgcgagttacgcttgtagagtcccggcaacttctcgcatgctctaccggccttccggtctgcgttacccgccgctgccctgccatggctcagcacttctccctgggcgcctgcgacgtggtcggcttcgatctcgaccacacgctgtgccgctacaacctgcccgagagcgcccggctcatttataacagctttgctcagttcttagtcaaggagaaaggatatgatgaggggctgctcactctgactccagaggactgggatttctgttgcaagggtttggcattggatctggaagatgggaccttcattaaacttgcagctgatggcactgtgctcagggcaagccacggcaccaagatgatgactccagaggcactgactgaagcctttggcaagaaggagtggaggcactgtgtgtcagacaaacgctgtacatcagacaagcccggtgtgtccgacattccttgctgctcaggaaagtgttatttctatgataactactttgacctgccgggggctcttctgtgtgccagagtggtggactctttaacaaagaaaattgtggattcttctttccggaaataaaaagaaatctaggcaaatatgtgcaccgctgtcctgagtctgtaagaaagtggcttcgacagctgaaggacgctgggaaaattactatgttgataactagttctcacagtgattactgtaaacttcttggcagttacatccttggggaggattttgcagacctttttgacattgtgattacaaacgcattaaagcctggattcttctcacatttcccaagccagaggcctttctacacactcgagaatgatgaagagaaggatgagctgccgtctctggataaaccgggctggtactcccaagggaacgctgcccacctttatgagcttctgaagaaaatgaccagcaagcctgaacccaaggtcgtttactttggtgacagcatgcattcagacattttcccagcccatcactatactaactgggagactgtccttattctcgaggagcttcaggggccagaaatggaaaagcctgaggaagcagagcctctggagaaaagaggaaaatatgaggcaccaatggtaaagcctctaaacaccttgtctaataagtggggctcatactttattgactcagtctccggacgggggagggcagaagactctgtggtttatacatggtcctctaagagaatcagcacctacagtacgattgcaattccaaacattgaatctattgcagagttacccctggactacaaatttacaagattttctacaaacaattcaaaaatggctggttactatcccttggtccatcataccttgggttctcaagacacagacagcaaaataatatctactgaaaaatgaaccaacctatgttcagtagacatactgtttaaaaaatgttagttttccacaacaatgcaaatgttttgtaagaacaatttttcaatgaaaattgaccaaatggacacttgtcagtctgtctttttaaaatataagtaattttatataaatccttttaatgtttaacagttcttattatattaatatttttagtaccctagactaaaagagaacatgactgatcttttctatgctggccgttttgaggattcttccaaggtgatagacaaatacatgcatctacctggtccagcagtttccaagcctgtccacagtctaatagcagagcagcatagaacttgaaaatgcaaatctgagtgcttcctcccaaacctacgaaatcagaaactttgggagtggggccagcaatggcttttaagcaaacttccagtaaatcgaatgctctattatgatttgagacctagtaagtaaaccctgttctgagtattcatacttttaattgaaagtgtaatctgaattgataacaccaaagtctaggttattgttccaggctattaggctttttgctatttcttttttcccccaaaatacataaagagacatgatttgttaagataatattacctcgttccttagtattaatttctcacattaggaagagttaaatttatacctattaaattcagctttgtccttggaaagggagctggaagacacagttgcttaggtacacttcaggttaggatatgttgtgctctgtgtggttttgataagttaacatgtgcatcagatcatgtatatataagatattcagattcagttgatgttttataagttcctgggtcctcttttgcctgttaacttcactgtacataaatggatgggattagaaaatattatcattatttttgtcttggaatttagcatattaacatgtgcactgaaaattttattttcttaaaatttccaaatattcagataaatcctaactgtactatgaagatgtgtttctcagggtaattaaaaaggaaacagaacttatttctttactatcagtatatttgatattgatcaataagaatacatatacagagatatatttgatatccaaaatgtcattagagtattataatgagcgcatgcaatcaagcctagggccaaataaatgctcttatctatctattatgtgctttaagtagagaattcactttgctttaaaataaagctatttttccttataaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaa

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