LOCUS       NM_001290812            2369 bp    mRNA    linear   ROD 20-NOV-2025
DEFINITION  Mus musculus adenosine deaminase-like (Adal), transcript variant 3,
            mRNA.
ACCESSION   NM_001290812
VERSION     NM_001290812.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2369)
  AUTHORS   Ogawa,A., Watanabe,S., Ozerova,I., Tsai,A.Y., Kuchitsu,Y.,
            Chong,H.B., Kawakami,T., Fuse,J., Han,W., Kudo,R., Naito,T.,
            Sato,K., Nakazawa,T., Saheki,Y., Hirayama,A., Stadler,P.F.,
            Arisawa,M., Araki,K., Bar-Peled,L., Taguchi,T., Sawa,S., Inaba,K.
            and Wei,F.Y.
  TITLE     Adenosine kinase and ADAL coordinate detoxification of modified
            adenosines to safeguard metabolism
  JOURNAL   Cell 188 (22), 6151-6169 (2025)
   PUBMED   40840445
REFERENCE   2  (bases 1 to 2369)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 2369)
  AUTHORS   Ingham,N.J., Pearson,S.A., Vancollie,V.E., Rook,V., Lewis,M.A.,
            Chen,J., Buniello,A., Martelletti,E., Preite,L., Lam,C.C.,
            Weiss,F.D., Powis,Z., Suwannarat,P., Lelliott,C.J., Dawson,S.J.,
            White,J.K. and Steel,K.P.
  TITLE     Mouse screen reveals multiple new genes underlying mouse and human
            hearing loss
  JOURNAL   PLoS Biol 17 (4), e3000194 (2019)
   PUBMED   30973865
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2369)
  AUTHORS   Lesscher,H.M.B., Bailey,A. and Vanderschuren,L.J.M.J.
  TITLE     Genetic Variability in Adenosine Deaminase-Like Contributes to
            Variation in Alcohol Preference in Mice
  JOURNAL   Alcohol Clin Exp Res 41 (7), 1271-1279 (2017)
   PUBMED   28449374
  REMARK    GeneRIF: genetically protective factor against alcohol consumption
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK048809.1, AK039677.1 and AK049697.1.
            
            Transcript Variant: This variant (3) contains alternate 5' exon
            structure, and it thus differs in its 5' UTR and initiates
            translation at a downstream in-frame start codon, and it also
            includes an additional in-frame exon in the 3' coding region,
            compared to variant 1. The encoded isoform (c) is shorter at the
            N-terminus, compared to variant 1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK039677.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-11                AK048809.1         2-12
            12-850              AK039677.1         2-840
            851-1738            AK039677.1         842-1729
            1739-2369           AK049697.1         1838-2468
FEATURES             Location/Qualifiers
     source          1..2369
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 60.37 cM"
     gene            1..2369
                     /gene="Adal"
                     /gene_synonym="4930578F03Rik; Mapda"
                     /note="adenosine deaminase-like"
                     /db_xref="GeneID:75894"
                     /db_xref="MGI:MGI:1923144"
     exon            1..182
                     /gene="Adal"
                     /gene_synonym="4930578F03Rik; Mapda"
                     /inference="alignment:Splign:2.1.0"
     exon            183..393
                     /gene="Adal"
                     /gene_synonym="4930578F03Rik; Mapda"
                     /inference="alignment:Splign:2.1.0"
     exon            394..467
                     /gene="Adal"
                     /gene_synonym="4930578F03Rik; Mapda"
                     /inference="alignment:Splign:2.1.0"
     misc_feature    439..441
                     /gene="Adal"
                     /gene_synonym="4930578F03Rik; Mapda"
                     /note="upstream in-frame stop codon"
     exon            468..594
                     /gene="Adal"
                     /gene_synonym="4930578F03Rik; Mapda"
                     /inference="alignment:Splign:2.1.0"
     CDS             475..1236
                     /gene="Adal"
                     /gene_synonym="4930578F03Rik; Mapda"
                     /EC_number="3.5.4.-"
                     /note="isoform c is encoded by transcript variant 3;
                     adenosine deaminase-like protein; N6-Methyl-AMP deaminase"
                     /codon_start=1
                     /product="N6-Methyl-AMP deaminase isoform c"
                     /protein_id="NP_001277741.1"
                     /db_xref="CCDS:CCDS79836.1"
                     /db_xref="GeneID:75894"
                     /db_xref="MGI:MGI:1923144"
                     /translation="

MAIDRRGGPTIARETVELAKEFFLSTENTVLGLDLSGDPTIGQANDFLEPLLEAKKAGLKLALHLAEIPNREKENQMLLSLLPDRIGHGTFLSASEAGALDQVDFVRQHQIPLVLEANAELYMCRAGPLLLRHIPTQNKSTSQVMNILWELCLTSNIKSQTVPSYDQHHFGFWYSIAHPSVICTDDKGVFATYLSQEYQLAAETFNLTPFQVWDLSYESINYIFACDNTRSELRKRWTHLKQKVLNCNEVNYF"

     misc_feature    <481..1185
                     /gene="Adal"
                     /gene_synonym="4930578F03Rik; Mapda"
                     /note="Superfamily of metallo-dependent hydrolases (also
                     called amidohydrolase superfamily) is a large group of
                     proteins that show conservation in their 3-dimensional
                     fold (TIM barrel) and in details of their active site. The
                     vast majority of the members have a...; Region:
                     metallo-dependent_hydrolases; cl00281"
                     /db_xref="CDD:469705"
     exon            595..675
                     /gene="Adal"
                     /gene_synonym="4930578F03Rik; Mapda"
                     /inference="alignment:Splign:2.1.0"
     exon            676..814
                     /gene="Adal"
                     /gene_synonym="4930578F03Rik; Mapda"
                     /inference="alignment:Splign:2.1.0"
     exon            815..922
                     /gene="Adal"
                     /gene_synonym="4930578F03Rik; Mapda"
                     /inference="alignment:Splign:2.1.0"
     exon            923..1023
                     /gene="Adal"
                     /gene_synonym="4930578F03Rik; Mapda"
                     /inference="alignment:Splign:2.1.0"
     exon            1024..2369
                     /gene="Adal"
                     /gene_synonym="4930578F03Rik; Mapda"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2346..2351
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Adal"
                     /gene_synonym="4930578F03Rik; Mapda"
                     /note="hexamer: ATTAAA"
     polyA_site      2367
                     /gene="Adal"
                     /gene_synonym="4930578F03Rik; Mapda"
                     /note="major polyA site"
ORIGIN      

acgtaatagccgcggtggataagcggcgtgcgccgcacgggtcccgggaccagcagcgccgcaaaggggtcgcgcacgtatccgtgtgcggcaagcgatcgtttgctgagggagctgctgtcgttggtgctctggaccgttcccgcccggcgctggcggcctcgcgggcccatgacgagaaggtgaaagtgatcccagagccacacatagtgcaagtcttgtttaaaatggcaatctgaaggaagtccagcacctcagagattcccactttgataagtttatcgtccaagccgagaataagagcaaagagaaacttttccttctactgaagtgatggaggccggacagcagtggcctgggaagacagacttttatctgcaattaccaaaagtgatgtttccagatgttccaagttatacatcagcttactactagtgctgaggacatcctgatggtgagacacagaggtatttgatggcaattgacagaagaggtggcccaacaatagccagggagactgtcgaacttgctaaagagttcttcctctctactgagaatacagttcttggccttgacctcagtggagatccaactataggacaagctaacgacttcttggagcctcttttagaagctaaaaaagcaggtctgaagttggcattgcatcttgccgagattccaaacagggaaaaagaaaatcagatgctgctgagtctgctccctgacagaattgggcatgggacattccttagcgcctctgaggcaggggccctggaccaagtggacttcgtgaggcaacaccagatacccctcgtgttggaagcaaatgcagagctctacatgtgcagggcaggccctcttctgctgaggcacatccccactcagaacaagtccacatcacaagtaatgaacattttgtgggaactttgtttgacctcaaacatcaaaagccagacagtcccttcttatgatcagcatcactttggattttggtacagcattgctcatccttctgtgatctgtactgatgataaaggtgtttttgcgacatacctttctcaagaatatcaactggcagcagaaacatttaatttgaccccatttcaggtgtgggatctgtcctatgaatccatcaactacatctttgcttgtgacaacaccagatctgaactgagaaaaaggtggactcacttgaagcagaaagtgttaaactgtaatgaggtgaactacttttgattatacattgtagccaagatcttcctgccctgtgtatcctactcagttactgcccacttgaaatgacttcccttgaatttgggtagccaagtatatatggggtctaactccagccccttcaactggtaagttcgaggttttgcatggctgcacttccatgctgcttttgctggagggttgtttagccctcatttcctagtgactcttgtttttacacttagtgctctttagacatttgtgaccgtgaaccttctgagaagttgaaactctttgtcttcagggaaatctctgttttgttaggtttaatcagactccttccacttccttttaccctttcaagtattaacttttttagactgttgatgttaggtcacagtgcacacctgaactgtttgtgtgggtgcctagttgtcaggtgactgtagtaacaaagctagaatctggctttgcccttctcctaatatctttagaaatatataacgggggttggagtaatcccttcagtttaagaaaagaaaagaacgactggcttgaagtaaaaaagacacatagctggagcttctgatttaaaatcaggttctatttggcttccttgtggaagctaccaggacctctacagtgctaggactctcttaaaagatctttgtagcaatggtagcattgtggcttctagggttcttagatcaccaataatcccatgcccacatgatagcctacaggtatagacttatataaacacacatataccatggccacattagccgactttagcagataacaaatgtcttggttggtttttttcacaagctagagtcatttgggaagagggaccctcaactgagaaaatattcccacaagattggcccaatcagtgcttgatcagttagtgggcccatgccacagagggtgataccacccttagggacatggttctgggtggaaagcaaaggaaatggaacaaggcacgaggagcaagccataggcaacactcctccatggcttcagctttagttcctgcctcaacttcccaggatgacagactacaaattataagaggaaataaccctttcctccttacattacatttggttatggtgttttgtcacagcattaaaaacactaaaacaataatg

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