LOCUS       NM_001290811            2475 bp    mRNA    linear   ROD 20-NOV-2025
DEFINITION  Mus musculus adenosine deaminase-like (Adal), transcript variant 2,
            mRNA.
ACCESSION   NM_001290811 XM_006500354
VERSION     NM_001290811.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2475)
  AUTHORS   Ogawa,A., Watanabe,S., Ozerova,I., Tsai,A.Y., Kuchitsu,Y.,
            Chong,H.B., Kawakami,T., Fuse,J., Han,W., Kudo,R., Naito,T.,
            Sato,K., Nakazawa,T., Saheki,Y., Hirayama,A., Stadler,P.F.,
            Arisawa,M., Araki,K., Bar-Peled,L., Taguchi,T., Sawa,S., Inaba,K.
            and Wei,F.Y.
  TITLE     Adenosine kinase and ADAL coordinate detoxification of modified
            adenosines to safeguard metabolism
  JOURNAL   Cell 188 (22), 6151-6169 (2025)
   PUBMED   40840445
REFERENCE   2  (bases 1 to 2475)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 2475)
  AUTHORS   Ingham,N.J., Pearson,S.A., Vancollie,V.E., Rook,V., Lewis,M.A.,
            Chen,J., Buniello,A., Martelletti,E., Preite,L., Lam,C.C.,
            Weiss,F.D., Powis,Z., Suwannarat,P., Lelliott,C.J., Dawson,S.J.,
            White,J.K. and Steel,K.P.
  TITLE     Mouse screen reveals multiple new genes underlying mouse and human
            hearing loss
  JOURNAL   PLoS Biol 17 (4), e3000194 (2019)
   PUBMED   30973865
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2475)
  AUTHORS   Lesscher,H.M.B., Bailey,A. and Vanderschuren,L.J.M.J.
  TITLE     Genetic Variability in Adenosine Deaminase-Like Contributes to
            Variation in Alcohol Preference in Mice
  JOURNAL   Alcohol Clin Exp Res 41 (7), 1271-1279 (2017)
   PUBMED   28449374
  REMARK    GeneRIF: genetically protective factor against alcohol consumption
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK048809.1, AK016299.1 and AK049697.1.
            
            On Mar 20, 2014 this sequence version replaced XM_006500354.1.
            
            Transcript Variant: This variant (2) contains alternate 5' exon
            structure, and it thus differs in its 5' UTR and initiates
            translation at a downstream in-frame start codon, compared to
            variant 1. The encoded isoform (b) is shorter at the N-terminus,
            compared to variant 1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK049697.1, AK016299.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-4                 AK048809.1         2-5
            5-1149              AK016299.1         2-1146
            1150-2475           AK049697.1         1143-2468
FEATURES             Location/Qualifiers
     source          1..2475
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 60.37 cM"
     gene            1..2475
                     /gene="Adal"
                     /gene_synonym="4930578F03Rik; Mapda"
                     /note="adenosine deaminase-like"
                     /db_xref="GeneID:75894"
                     /db_xref="MGI:MGI:1923144"
     exon            1..239
                     /gene="Adal"
                     /gene_synonym="4930578F03Rik; Mapda"
                     /inference="alignment:Splign:2.1.0"
     exon            240..450
                     /gene="Adal"
                     /gene_synonym="4930578F03Rik; Mapda"
                     /inference="alignment:Splign:2.1.0"
     misc_feature    449..451
                     /gene="Adal"
                     /gene_synonym="4930578F03Rik; Mapda"
                     /note="upstream in-frame stop codon"
     exon            451..511
                     /gene="Adal"
                     /gene_synonym="4930578F03Rik; Mapda"
                     /inference="alignment:Splign:2.1.0"
     CDS             461..1342
                     /gene="Adal"
                     /gene_synonym="4930578F03Rik; Mapda"
                     /EC_number="3.5.4.-"
                     /note="isoform b is encoded by transcript variant 2;
                     adenosine deaminase-like protein; N6-Methyl-AMP deaminase"
                     /codon_start=1
                     /product="N6-Methyl-AMP deaminase isoform b"
                     /protein_id="NP_001277740.1"
                     /db_xref="CCDS:CCDS71121.1"
                     /db_xref="GeneID:75894"
                     /db_xref="MGI:MGI:1923144"
                     /translation="

MFQVIHQLTTSAEDILMVTKDVIKEFADDGVKYLELRSTPREENATGMTRKTYVESVLEGIKQCKQENLDIDVRYLMAIDRRGGPTIARETVELAKEFFLSTENTVLGLDLSGDPTIGQANDFLEPLLEAKKAGLKLALHLAEIPNREKENQMLLSLLPDRIGHGTFLSASEAGALDQVDFVRQHQIPLELCLTSNIKSQTVPSYDQHHFGFWYSIAHPSVICTDDKGVFATYLSQEYQLAAETFNLTPFQVWDLSYESINYIFACDNTRSELRKRWTHLKQKVLNCNEVNYF"

     misc_feature    461..1291
                     /gene="Adal"
                     /gene_synonym="4930578F03Rik; Mapda"
                     /note="Adenosine/AMP deaminase. Adenosine deaminases
                     (ADAs) are present in pro- and eukaryotic organisms and
                     catalyze the zinc dependent irreversible deamination of
                     adenosine nucleosides to inosine nucleosides and ammonia.
                     The eukaryotic AMP deaminase...; Region: ADA_AMPD;
                     cd00443"
                     /db_xref="CDD:238250"
     exon            512..599
                     /gene="Adal"
                     /gene_synonym="4930578F03Rik; Mapda"
                     /inference="alignment:Splign:2.1.0"
     exon            600..681
                     /gene="Adal"
                     /gene_synonym="4930578F03Rik; Mapda"
                     /inference="alignment:Splign:2.1.0"
     exon            682..808
                     /gene="Adal"
                     /gene_synonym="4930578F03Rik; Mapda"
                     /inference="alignment:Splign:2.1.0"
     exon            809..889
                     /gene="Adal"
                     /gene_synonym="4930578F03Rik; Mapda"
                     /inference="alignment:Splign:2.1.0"
     exon            890..1028
                     /gene="Adal"
                     /gene_synonym="4930578F03Rik; Mapda"
                     /inference="alignment:Splign:2.1.0"
     exon            1029..1129
                     /gene="Adal"
                     /gene_synonym="4930578F03Rik; Mapda"
                     /inference="alignment:Splign:2.1.0"
     exon            1130..2475
                     /gene="Adal"
                     /gene_synonym="4930578F03Rik; Mapda"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2452..2457
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Adal"
                     /gene_synonym="4930578F03Rik; Mapda"
                     /note="hexamer: ATTAAA"
     polyA_site      2473
                     /gene="Adal"
                     /gene_synonym="4930578F03Rik; Mapda"
                     /note="major polyA site"
ORIGIN      

acgtaatagccgcggtggataagcggcgtgcgccgcacgggtcccgggaccagcagcgccgcaaaggggtcgcgcacgtatccgtgtgcggcaagcgatcgtttgctgagggagctgctgtcgttggtgctctggaccgttcccgcccggcgctggcggcctcgcgggcccatgacgagaaggtgctcagcaatactgcacacagaacacttaccgagctcccgctcatccgcttctgggtgaaagtgatcccagagccacacatagtgcaagtcttgtttaaaatggcaatctgaaggaagtccagcacctcagagattcccactttgataagtttatcgtccaagccgagaataagagcaaagagaaacttttccttctactgaagtgatggaggccggacagcagtggcctgggaagacagacttttatctgcaattaccaaaagtgatgtttccagatgttccaagttatacatcagcttactactagtgctgaggacatcctgatggtcacaaaagacgtcattaaggaatttgcagatgatggtgtcaagtacctggagctgaggagcactcccagagaagaaaatgctactggaatgactaggaagacttacgtggaatccgtccttgaaggcataaaacaatgcaaacaggaaaacttagatattgatgttaggtatttgatggcaattgacagaagaggtggcccaacaatagccagggagactgtcgaacttgctaaagagttcttcctctctactgagaatacagttcttggccttgacctcagtggagatccaactataggacaagctaacgacttcttggagcctcttttagaagctaaaaaagcaggtctgaagttggcattgcatcttgccgagattccaaacagggaaaaagaaaatcagatgctgctgagtctgctccctgacagaattgggcatgggacattccttagcgcctctgaggcaggggccctggaccaagtggacttcgtgaggcaacaccagatacccctcgaactttgtttgacctcaaacatcaaaagccagacagtcccttcttatgatcagcatcactttggattttggtacagcattgctcatccttctgtgatctgtactgatgataaaggtgtttttgcgacatacctttctcaagaatatcaactggcagcagaaacatttaatttgaccccatttcaggtgtgggatctgtcctatgaatccatcaactacatctttgcttgtgacaacaccagatctgaactgagaaaaaggtggactcacttgaagcagaaagtgttaaactgtaatgaggtgaactacttttgattatacattgtagccaagatcttcctgccctgtgtatcctactcagttactgcccacttgaaatgacttcccttgaatttgggtagccaagtatatatggggtctaactccagccccttcaactggtaagttcgaggttttgcatggctgcacttccatgctgcttttgctggagggttgtttagccctcatttcctagtgactcttgtttttacacttagtgctctttagacatttgtgaccgtgaaccttctgagaagttgaaactctttgtcttcagggaaatctctgttttgttaggtttaatcagactccttccacttccttttaccctttcaagtattaacttttttagactgttgatgttaggtcacagtgcacacctgaactgtttgtgtgggtgcctagttgtcaggtgactgtagtaacaaagctagaatctggctttgcccttctcctaatatctttagaaatatataacgggggttggagtaatcccttcagtttaagaaaagaaaagaacgactggcttgaagtaaaaaagacacatagctggagcttctgatttaaaatcaggttctatttggcttccttgtggaagctaccaggacctctacagtgctaggactctcttaaaagatctttgtagcaatggtagcattgtggcttctagggttcttagatcaccaataatcccatgcccacatgatagcctacaggtatagacttatataaacacacatataccatggccacattagccgactttagcagataacaaatgtcttggttggtttttttcacaagctagagtcatttgggaagagggaccctcaactgagaaaatattcccacaagattggcccaatcagtgcttgatcagttagtgggcccatgccacagagggtgataccacccttagggacatggttctgggtggaaagcaaaggaaatggaacaaggcacgaggagcaagccataggcaacactcctccatggcttcagctttagttcctgcctcaacttcccaggatgacagactacaaattataagaggaaataaccctttcctccttacattacatttggttatggtgttttgtcacagcattaaaaacactaaaacaataatg

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