LOCUS       NM_001285898            2161 bp    mRNA    linear   ROD 05-AUG-2023
DEFINITION  Mus musculus calreticulin 4 (Calr4), transcript variant 5, mRNA.
ACCESSION   NM_001285898
VERSION     NM_001285898.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Diaz Del Moral S, Barrena S, Hernandez-Torres F, Aranega A,
            Villaescusa JM, Gomez Doblas JJ, Franco D, Jimenez-Navarro M,
            Munoz-Chapuli R and Carmona R.
  TITLE     Deletion of the Wilms' Tumor Suppressor Gene in the Cardiac
            Troponin-T Lineage Reveals Novel Functions of WT1 in Heart
            Development
  JOURNAL   Front Cell Dev Biol 9, 683861 (2021)
   PUBMED   34368133
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2161)
  AUTHORS   Ansarullah, Jain C, Far FF, Homberg S, Wissmiller K, von Hahn FG,
            Raducanu A, Schirge S, Sterr M, Bilekova S, Siehler J, Wiener J,
            Oppenlander L, Morshedi A, Bastidas-Ponce A, Collden G, Irmler M,
            Beckers J, Feuchtinger A, Grzybek M, Ahlbrecht C, Feederle R,
            Plettenburg O, Muller TD, Meier M, Tschop MH, Coskun U and Lickert
            H.
  TITLE     Inceptor counteracts insulin signalling in beta-cells to control
            glycaemia
  JOURNAL   Nature 590 (7845), 326-331 (2021)
   PUBMED   33505018
  REMARK    Erratum:[Nature. 2021 Apr;592(7852):E1. PMID: 33712809]
REFERENCE   3  (bases 1 to 2161)
  AUTHORS   Dey S and Matsunami H.
  TITLE     Calreticulin chaperones regulate functional expression of
            vomeronasal type 2 pheromone receptors
  JOURNAL   Proc Natl Acad Sci U S A 108 (40), 16651-16656 (2011)
   PUBMED   21933956
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL627103.13, AK077094.1, AK139540.1 and BG083829.2.
            
            Transcript Variant: This variant (5) has alternate 5' exon
            structure and it thus differs in the 5' UTR and initiates
            translation at a downstream in-frame start codon, compared to
            variant 1. The encoded isoform (c) is shorter at the N-terminus,
            compared to isoform a. Variants 3, 4 and 5 all encode isoform c.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC044740.2, SRR1660813.189233.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849384, SAMN01164131
                                           [ECO:0000348]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-371               AL627103.13        87877-88247
            372-1758            AK077094.1         212-1598
            1759-2141           AK139540.1         1509-1891
            2142-2161           BG083829.2         516-535
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="4"
                     /map="4 51.1 cM"
     gene            1..2161
                     /gene="Calr4"
                     /gene_synonym="4933403L16Rik"
                     /note="calreticulin 4"
                     /db_xref="GeneID:108802"
                     /db_xref="MGI:MGI:2140435"
     exon            1..371
                     /gene="Calr4"
                     /gene_synonym="4933403L16Rik"
                     /inference="alignment:Splign:2.1.0"
     misc_feature    335..337
                     /gene="Calr4"
                     /gene_synonym="4933403L16Rik"
                     /note="upstream in-frame stop codon"
     exon            372..473
                     /gene="Calr4"
                     /gene_synonym="4933403L16Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            474..677
                     /gene="Calr4"
                     /gene_synonym="4933403L16Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             644..1585
                     /gene="Calr4"
                     /gene_synonym="4933403L16Rik"
                     /note="isoform c is encoded by transcript variant 5"
                     /codon_start=1
                     /product="calreticulin 4 isoform c"
                     /protein_id="NP_001272827.1"
                     /db_xref="CCDS:CCDS71441.1"
                     /db_xref="GeneID:108802"
                     /db_xref="MGI:MGI:2140435"
                     /translation="

MHSESQYYIMFGPDICGFGNNRLQVILSHKGKYHENNKTLKCRINKDTHLYTLILRPNATYEVKIDNQKVTSGGLEDDWDFLPPKKIKDPYARKPRKWDERQQIEDPDDKKPEDWEDSEFIPDPDAKKPDDWNEAMDGVWEGPLIPNVKYMGEWKPRIIDNPNYQGEWIHPEIDNPKYRPDPTIGHYHNISVLGLDLWQVKSGSIFDNFLLTNDEEFAEEVGNMTWGLRKDVEQQWRELYEEMEKQKEAEETKKKREKERARQEDVWGLDEEDEEDAEEDDEEEAEERLKQEGSAARASLDQKEAHLDQKDEL"

     misc_feature    <644..1276
                     /gene="Calr4"
                     /gene_synonym="4933403L16Rik"
                     /note="Calreticulin family; Region: Calreticulin;
                     pfam00262"
                     /db_xref="CDD:459737"
     exon            678..772
                     /gene="Calr4"
                     /gene_synonym="4933403L16Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            773..982
                     /gene="Calr4"
                     /gene_synonym="4933403L16Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            983..1096
                     /gene="Calr4"
                     /gene_synonym="4933403L16Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1097..1240
                     /gene="Calr4"
                     /gene_synonym="4933403L16Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1241..1333
                     /gene="Calr4"
                     /gene_synonym="4933403L16Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1334..2161
                     /gene="Calr4"
                     /gene_synonym="4933403L16Rik"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      

gtcgaaaactgcaagggagagtccatagagtttaagcaacagaaaggggccaggagaaattgattgttcgtgataatcattactaatttaaggagtttgaaatgttttgctagtctcttccctgagggagcaggttttactattttacttcacaaagcataagtgtgtgcaagggcttagtcctcaggggcttattttccatctacttttgtgactatagcgcttcttgccctacaaccgcaccctcccaatggcagttttcaggacttaacccccgcgaatgcagtgttggtggaaaagtggctcaggtgcttggcatttgagcagctcctcctgacaggacataaatagtgggttccaggtactgtgtgatggatggacaaaacggtgggtacaatctaaacatcagtcagattacggccaattccagctagcctcgggaaaattttatcaagataaagagagagataaaggtctccagaccacagaagatgccaagttctacgccctttccaccaggtttaagccattcagcaatgagaatgagaccttggtggttcagttttcagtaaaacacgagcaaggcatcgattgcggcggtggctacgtgaaactctttcctgccgcactgaaccaggaggacatgcactcagaatcccagtattacatcatgttcggcccggacatctgtggctttggcaacaacagactacaggtcatcctttctcacaaagggaaataccatgagaacaacaagaccctcaagtgcaggattaataaagacactcacctgtacactctgatccttcgccctaatgctacttatgaggttaaaattgacaaccagaaggtgacaagtggaggactggaagatgactgggacttcttgcctcccaagaaaataaaagacccctacgccaggaaaccaaggaaatgggatgaacgacaacagatagaggatcctgacgataagaaacctgaggactgggaggactctgaattcatcccagacccggatgccaagaagccagatgactggaatgaggcgatggatggagtgtgggaagggcccttgataccaaacgtcaagtacatgggagaatggaaacctcggatcattgacaaccccaattaccagggcgagtggattcacccagagatagacaaccccaaatacaggcctgaccccaccattggtcactatcacaacattagtgtccttggtctggatctttggcaggtgaaatcaggcagcatctttgacaatttccttctaacaaatgatgaagagtttgctgaagaggttggaaatatgacctggggattaagaaaggatgtggagcagcagtggagagagctgtatgaggaaatggagaaacagaaggaggcggaggagaccaagaagaagagggaaaaggagagagccaggcaagaggacgtctggggactagatgaggaggatgaggaggacgctgaagaggacgacgaggaggaagcagaggaaaggctcaagcaggaaggcagtgcagcgagggcgtctctggaccagaaggaagcccacttggatcagaaggatgaactgtaactgcacagacactgggtgtggccgaagtcagagacggtgcttagagagggaaatgggccctcgcttagcaaattatgggaagtaaaacgactcattctttttagataaactttggatttatagttctactccccttccctgctctatttgctcattggtagaaagggaatgataattttatctcatgaaatggcccttgggactgaatgaggcaatgcctgtaacatgcccaaagtaaatacttgtaaaaaaaatgtagtagttaaaactactatcattatcatggtgtttatgaaattaattgttggtaaataagcttctatataaacttggttcttactttcaactggggtttttcttttgtttaaatatatgcaattcttagtatttatggagcagtgctaacagttgaacccaatgtttccttttttttttttttttttaaatgtattgagcacctaaagccaaaagcagaacatcctttggacgtttggaaaaatacttcatgaaagtcaccttgtttgcctgctgctcttgccttatgtttaaacaattgttacttgtttgcaagtttgc

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