LOCUS       XM_073951019            3438 bp    mRNA    linear   VRT 12-MAY-2025
DEFINITION  PREDICTED: Danio rerio piwi-like RNA-mediated gene silencing 2
            (piwil2), transcript variant X1, mRNA.
ACCESSION   XM_073951019
VERSION     XM_073951019.1
DBLINK      BioProject: PRJNA1248012
KEYWORDS    RefSeq.
SOURCE      Danio rerio (zebrafish)
  ORGANISM  Danio rerio
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Ostariophysi;
            Cypriniformes; Danionidae; Danioninae; Danio.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_133180) annotated using gene prediction method: Gnomon,
            supported by mRNA and EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_049306965.1-RS_2025_04
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.3
            Annotation Method           :: Best-placed RefSeq; Gnomon;
                                           cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 04/11/2025
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3438
                     /organism="Danio rerio"
                     /mol_type="mRNA"
                     /strain="Tuebingen"
                     /isolation_source="Zebrafish international resource
                     center"
                     /db_xref="taxon:7955"
                     /chromosome="5"
                     /sex="male"
                     /tissue_type="Fibroblasts and whole tissue"
                     /dev_stage="adult"
                     /ecotype="United States"
                     /geo_loc_name="USA"
                     /collection_date="2022-07-19"
                     /collected_by="National Human Genome Research Institute"
                     /genotype="Double heterozygous"
     gene            1..3438
                     /gene="piwil2"
                     /gene_synonym="sb:eu354; si:dkey-88f5.1; zili"
                     /note="piwi-like RNA-mediated gene silencing 2; Derived by
                     automated computational analysis using gene prediction
                     method: Gnomon. Supporting evidence includes similarity
                     to: 1 mRNA, 8 ESTs, 6248 long SRA reads, 26 Proteins"
                     /db_xref="GeneID:572134"
                     /db_xref="ZFIN:ZDB-GENE-060526-345"
     misc_feature    1
                     /gene="piwil2"
                     /gene_synonym="sb:eu354; si:dkey-88f5.1; zili"
                     /experiment="COORDINATES: cap analysis [ECO:0007248]"
                     /note="transcription start site"
     CDS             93..3233
                     /gene="piwil2"
                     /gene_synonym="sb:eu354; si:dkey-88f5.1; zili"
                     /codon_start=1
                     /product="piwi-like protein 2 isoform X1"
                     /protein_id="XP_073807120.1"
                     /db_xref="GeneID:572134"
                     /db_xref="ZFIN:ZDB-GENE-060526-345"
                     /translation="

MDPKRPTFPSPPGVIRAPWQQSTEDQSQLLDQPSLGRARGLIMPIDEPLPGRGRAFSVLGEMPVRFGRGITQSIAAEPLVGMARGVRLPMEEGGFGRGRGFLLPTPEPTVGIGRGAAIGPVPTLDIQKAEVEEKMPELQAEVAPTVAKVGSPGTGSSLVSMFRGLGIEPGKTWGRGAAPVGRGAAGDMGADLQPKPTIIGASLTPEREEVRSEESISFLGRGFTGFGRAAMPHMTVGRGPIGPLSPSPSVAAPFSLISASSSSEDAPVAPGTPPKVEVKIETVKEPLQKIGTKGSPIPIGSNYIPICCKNDAVFQYHVTFTPNVESLSMRFGMMKEHRPTTGEVVAFDGSILYLPKRLEEVVHLKAERKTDNQEIDIKIQLTKILPPSSDLCIPFYNVVLRRVMKILGLKLVGRNHYDPNAVVILGKHRLQVWPGYSTSIKHTDGGLYLVVDVSHKVLRNDSVLDVMNLIYQGSRESFQDECTKEFVGSIVITRYNNRTYRIDDIEWSKSPKDTFTLADGSVTTFVDYYRKNYGITIKELDQPLLIHRPKERSRPGGKVITGEILLLPELSFMTGIPEKMRKDFRAMKDLTMHINVGAQQHTQSLKQLLHNINSNNEALSELGRWGLSISQEILVTQGRTLPSETICLHSASFVTSPAVDWSRELVRDPSISTVPLNCWAVFYPRRATDQAEELVTTFSRVAGPMGMRVERPIRVELRDDRTETFVKSIHSQLTSEPRVQLVVCIMTGNRDDLYSAIKKLCCIQSPVPSQAINVRTISQPQKLRSVAQKILLQINCKLGGELWTVNVPLKYLMVIGVDVHHDTSKKSRSVMGFVASLNSMLTKWYSRVTFQMPNEEIINGFRVCLLAALQKYYEVNHAFPEKIVIYRDGVSDGQLKTVEHYEIPQILKCFETIPNYEPKLAFIVVQKRISTTLYSYGSDHFGTPSPGTVLDHTVTNRDWVDFYLMAHSIRQGCGLPTHYITVYNTANLTPDHLQRLTFKMCHLYWNWPGTIRVPAPCKYAHKLAFLSGQYLHSEPAIQLSEKLFFL"

     misc_feature    1326..1469
                     /gene="piwil2"
                     /gene_synonym="sb:eu354; si:dkey-88f5.1; zili"
                     /note="Argonaute linker 1 domain; Region: ArgoL1;
                     pfam08699"
                     /db_xref="CDD:462567"
     misc_feature    1473..1886
                     /gene="piwil2"
                     /gene_synonym="sb:eu354; si:dkey-88f5.1; zili"
                     /note="This domain is named PAZ after the proteins Piwi
                     Argonaut and Zwille; Region: PAZ; smart00949"
                     /db_xref="CDD:198017"
     misc_feature    order(1602..1604,1641..1643,1665..1667,1677..1679,
                     1731..1733,1782..1784,1788..1790)
                     /gene="piwil2"
                     /gene_synonym="sb:eu354; si:dkey-88f5.1; zili"
                     /note="nucleic acid-binding interface [nucleotide
                     binding]; other site"
                     /db_xref="CDD:239211"
     misc_feature    1845..3179
                     /gene="piwil2"
                     /gene_synonym="sb:eu354; si:dkey-88f5.1; zili"
                     /note="PIWI domain, Piwi-like subfamily found in
                     eukaryotes. This domain is found in Piwi and closely
                     related proteins, where it is believed to perform a
                     crucial role in germline cells, via RNA silencing. RNA
                     silencing refers to a group of related...; Region:
                     Piwi_piwi-like_Euk; cd04658"
                     /db_xref="CDD:240016"
     misc_feature    order(2352..2354,2364..2366,2400..2411,2418..2420,
                     2448..2450,2457..2459,2469..2471,2481..2483)
                     /gene="piwil2"
                     /gene_synonym="sb:eu354; si:dkey-88f5.1; zili"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240016"
     misc_feature    order(2544..2546,2550..2552,2754..2756,3153..3155)
                     /gene="piwil2"
                     /gene_synonym="sb:eu354; si:dkey-88f5.1; zili"
                     /note="active site"
                     /db_xref="CDD:240016"
     polyA_site      3438
                     /gene="piwil2"
                     /gene_synonym="sb:eu354; si:dkey-88f5.1; zili"
                     /experiment="COORDINATES: polyA evidence [ECO:0006239]"
ORIGIN      

agtttacccgggcatttggaggagcgaacagtgcattcaccgtctctcacaggtgtcaaacagaattacagtgaagtgcaacagaggaagaaatggatccaaaacgaccaacctttcccagtcccccaggggtcatccgggcaccatggcagcagtcgacagaggatcagagtcaacttttagatcagccctcattgggacgggccagaggtcttatcatgcccatagatgaacctcttcctggacgaggtagagcttttagtgtactcggagaaatgccggtgagatttggtagagggataactcagtctatagctgctgagcctctggtgggcatggctagaggtgtgaggttacctatggaagagggaggttttgggcgaggacgaggttttttgctgcccactcctgagcccactgttgggatagggagaggggctgctattggaccagtgcctacactggacattcaaaaagcagaggtggaggaaaagatgcctgagctgcaagcggaggtggctccaactgttgccaaggttggttcccctgggactggatcgtcactggtgtccatgttcaggggtctgggaattgagccaggaaagacatggggtagaggagctgcacctgttggacggggtgcagcaggcgatatgggggcagatttgcagccaaaacccaccatcattggagcatctctgactccagaaagagaagaggttcgctcagaagaatcgatcagtttcctgggaagaggctttactggttttggcagagcagcaatgcctcatatgactgttgggcgtggaccgattggcccattaagtccttcaccttctgtcgctgcccctttctctttgatttctgcttcctcatcctctgaagatgccccggttgctccaggaacacctcctaaagttgaagtcaaaattgagacagtgaaggagcctctgcagaagataggaactaaaggcagtcccatacccattggatcaaactacatccctatctgctgcaaaaacgatgcagtgtttcagtaccatgttacttttacgccgaacgttgaatcacttagcatgcgctttggaatgatgaaggagcatcggcccactactggagaagtagtggcttttgacggatctatcctctacctgccaaaacgtctggaagaggtggttcatctaaaagctgaaaggaaaaccgacaatcaggagatcgacatcaagatccaattgacgaagattctgcctcccagctctgacctttgtatcccgttttacaacgtggtcttaaggagagtgatgaaaatactggggctgaagctggtgggcagaaaccattacgaccccaatgcggttgtgatcctgggcaaacacaggcttcaggtgtggcctggatactccaccagtattaagcacactgacggtggcctctacctggttgtggatgtctcgcataaggtgctgcgaaacgactctgtgctggatgtcatgaatctgatttaccaaggaagccgtgagagctttcaggatgaatgcacaaaggagtttgtgggttcaatcgtcatcacacgctataataaccgcacttaccgtattgatgacatcgagtggtccaagtctcccaaagacactttcaccttggctgatggatctgtgaccacgtttgtcgattactacagaaagaactatgggattaccataaaggaactggaccaaccgctgctcattcatcgtccaaaagagaggtccagaccaggaggaaaggttataacaggtgaaatccttctgttgccggagctttccttcatgactggcatccctgagaaaatgaggaaagacttcagggccatgaaggatctgaccatgcacatcaatgtgggagctcagcaacacacccagagtctcaagcagctgctgcacaacatcaacagcaacaatgaagctctctcagagctgggccgctggggcctcagcatcagccaggagattctagtgactcaaggaagaactctgcccagtgagaccatctgcttgcattcagcctcttttgtcacatctccggcagtagactggtccagagagttggttcgagacccctccatcagcactgtccctctgaattgctgggctgttttctatcccagacgggcaaccgatcaggctgaggagctggtgaccactttttcaagggtagctggtcctatgggcatgagagtggaacgacccatccgtgtagagttgcgcgatgatcgaaccgagaccttcgtcaagagcattcactctcaactcaccagcgagcctcgtgtacagctagtcgtgtgcatcatgacaggaaacagagatgacctgtatagtgccatcaaaaagctttgctgcatccagagtccagtaccatcacaggcaatcaacgttcggaccatctcacagccgcagaagctccgcagtgttgctcaaaagatccttttgcagattaactgcaagcttggaggagagctgtggacggtgaacgttcctctgaaatacttgatggtgattggagtggatgttcaccatgataccagcaagaagagcagatctgtcatgggcttcgtggcaagtctcaacagcatgttgaccaaatggtactccagggtgaccttccaaatgcctaatgaggagattatcaacggctttagagtgtgtcttctcgctgccctgcagaaatactacgaggtgaaccatgccttccctgagaagattgtcatctatcgcgacggtgtgtctgacggacagctgaagaccgtggagcactacgagatccctcagattctgaaatgcttcgagaccatcccaaactacgagcccaagcttgcattcatcgtggtgcagaaaagaatcagcacaaccctgtactcctacgggtcagaccacttcgggacaccctcgccaggaactgtgctggaccacactgtgacaaacagagactgggtggacttctacctaatggcacattccattcgacagggttgtggcctgcccactcattacataacagtgtacaacaccgcaaacctcacccctgaccacctgcagaggttgaccttcaagatgtgtcacctgtactggaactggcctggaacaatacgcgttcctgcaccgtgcaaatatgcccacaaactggctttcctgtctggccagtacctccattcagagcctgcaatccagctgtcagagaaactgttttttctgtgaatgaaaggcattcagcatctcaacagaaccctggatgggtcagacctttgactttcgttcttagttttgttttaatcctgttcttaggaaagtgctaatacgtgttgtttacatgttaaaaaagaaatcctccaagttttgtttttgtgatttttagtttgtttttgtggagtgttaataaagtgtcacactttttcccccctca

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