LOCUS       NM_201465               2917 bp    mRNA    linear   VRT 08-OCT-2023
DEFINITION  Danio rerio calreticulin 3b (calr3b), mRNA.
ACCESSION   NM_201465
VERSION     NM_201465.3
KEYWORDS    RefSeq.
SOURCE      Danio rerio (zebrafish)
  ORGANISM  Danio rerio
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Ostariophysi;
            Cypriniformes; Danionidae; Danioninae; Danio.
REFERENCE   1  (bases 1 to 2917)
  AUTHORS   Grimholt U.
  TITLE     Whole genome duplications have provided teleosts with many roads to
            peptide loaded MHC class I molecules
  JOURNAL   BMC Evol Biol 18 (1), 25 (2018)
   PUBMED   29471808
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2917)
  AUTHORS   Lim KH, Chang YC, Chiang YH, Lin HC, Chang CY, Lin CS, Huang L,
            Wang WT, Gon-Shen Chen C, Chou WC and Kuo YY.
  TITLE     Expression of CALR mutants causes mpl-dependent thrombocytosis in
            zebrafish
  JOURNAL   Blood Cancer J 6 (10), e481 (2016)
   PUBMED   27716741
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2917)
  AUTHORS   Shan Y, Zhang Y, Zhuo X, Li X, Peng J and Fang W.
  TITLE     Matrix metalloproteinase-9 plays a role in protecting zebrafish
            from lethal infection with Listeria monocytogenes by enhancing
            macrophage migration
  JOURNAL   Fish Shellfish Immunol 54, 179-187 (2016)
   PUBMED   27068748
REFERENCE   4  (bases 1 to 2917)
  AUTHORS   Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I,
            Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y and
            Bobe J.
  TITLE     Gene evolution and gene expression after whole genome duplication
            in fish: the PhyloFish database
  JOURNAL   BMC Genomics 17, 368 (2016)
   PUBMED   27189481
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2917)
  AUTHORS   Singh SK, Sethi S, Aravamudhan S, Kruger M and Grabher C.
  TITLE     Proteome mapping of adult zebrafish marrow neutrophils reveals
            partial cross species conservation to human peripheral neutrophils
  JOURNAL   PLoS One 8 (9), e73998 (2013)
   PUBMED   24019943
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2917)
  AUTHORS   Le X, Pugach EK, Hettmer S, Storer NY, Liu J, Wills AA, DiBiase A,
            Chen EY, Ignatius MS, Poss KD, Wagers AJ, Langenau DM and Zon LI.
  TITLE     A novel chemical screening strategy in zebrafish identifies common
            pathways in embryogenesis and rhabdomyosarcoma development
  JOURNAL   Development 140 (11), 2354-2364 (2013)
   PUBMED   23615277
REFERENCE   7  (bases 1 to 2917)
  AUTHORS   Cuello S, Ximenez-Embun P, Ruppen I, Schonthaler HB, Ashman K,
            Madrid Y, Luque-Garcia JL and Camara C.
  TITLE     Analysis of protein expression in developmental toxicity induced by
            MeHg in zebrafish
  JOURNAL   Analyst 137 (22), 5302-5311 (2012)
   PUBMED   23014190
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            FQ377641.15, EH579061.1, CABZ01066723.1, EH602296.1 and
            CABZ01066724.1.
            
            On Jun 19, 2015 this sequence version replaced NM_201465.2.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: GFIL01002926.1, GDQQ01000357.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA4476742, SAMEA4476785
                                           [ECO:0000348]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-37                FQ377641.15        1488-1524           c
            38-200              EH579061.1         30-192
            201-220             CABZ01066723.1     11786-11805
            221-315             EH579061.1         213-307
            316-415             CABZ01066723.1     13779-13878
            416-615             EH579061.1         408-607
            616-787             CABZ01066723.1     16121-16292
            788-902             EH579061.1         780-894
            903-916             CABZ01066723.1     19209-19222
            917-948             EH602296.1         264-295             c
            949-949             CABZ01066723.1     19359-19359
            950-1143            EH602296.1         69-262              c
            1144-1144           CABZ01066723.1     19943-19943
            1145-1176           EH602296.1         36-67               c
            1177-2917           CABZ01066724.1     859-2599
FEATURES             Location/Qualifiers
     source          1..2917
                     /organism="Danio rerio"
                     /mol_type="mRNA"
                     /strain="Tuebingen"
                     /db_xref="taxon:7955"
                     /chromosome="2"
                     /map="2"
     gene            1..2917
                     /gene="calr3b"
                     /gene_synonym="calr3; calrl; id:ibd1087; wu:fc59g10"
                     /note="calreticulin 3b"
                     /db_xref="GeneID:321315"
                     /db_xref="ZFIN:ZDB-GENE-030131-9907"
     exon            1..191
                     /gene="calr3b"
                     /gene_synonym="calr3; calrl; id:ibd1087; wu:fc59g10"
                     /inference="alignment:Splign:2.1.0"
     misc_feature    86..88
                     /gene="calr3b"
                     /gene_synonym="calr3; calrl; id:ibd1087; wu:fc59g10"
                     /note="upstream in-frame stop codon"
     CDS             98..1351
                     /gene="calr3b"
                     /gene_synonym="calr3; calrl; id:ibd1087; wu:fc59g10"
                     /note="calreticulin like"
                     /codon_start=1
                     /product="calreticulin 3b precursor"
                     /protein_id="NP_958873.3"
                     /db_xref="GeneID:321315"
                     /db_xref="ZFIN:ZDB-GENE-030131-9907"
                     /translation="

MQISLLQLISAALLALAANAEVYLNEQFLDGDAWRSRWVNSEHKSDYGQFKLTAGNFYGDAEKDKGLQTSQDARFYATSVRFEPFSNEGKTLVIQFTVKHEQKIDCGGGYVKVFPADLNQADMHGDSQYYIMFGPDICGYSTKKVHVIFNYKGQNHLIKKDIKCKDDELTHLYTLILNPDQTYEVKIDNEKVESGSLEEDWDFLPPKKIKDPEAKKPEDWDDRAKIDDETDTKPEDWDKPENIPDPDAKKPEDWDEDMDGEWEPAMIPNPEYKGEWKPKQIDNPNYKGAWVHPEIDNPEHVPDDQIYKFDNIGVLGLDLWQVKSGTIFDNFFISDDVKEAEEFGNETWGATKGPEKKMKEEQEEKKRKEEEEKNKEQNTEAADEEEEDEGEEEEEEDETEEPQEDEGDEDVLQKDEL"

     sig_peptide     98..157
                     /gene="calr3b"
                     /gene_synonym="calr3; calrl; id:ibd1087; wu:fc59g10"
                     /inference="COORDINATES: ab initio prediction:SignalP:4.0"
     misc_feature    173..1096
                     /gene="calr3b"
                     /gene_synonym="calr3; calrl; id:ibd1087; wu:fc59g10"
                     /note="Calreticulin family; Region: Calreticulin;
                     pfam00262"
                     /db_xref="CDD:459737"
     exon            192..293
                     /gene="calr3b"
                     /gene_synonym="calr3; calrl; id:ibd1087; wu:fc59g10"
                     /inference="alignment:Splign:2.1.0"
     exon            294..497
                     /gene="calr3b"
                     /gene_synonym="calr3; calrl; id:ibd1087; wu:fc59g10"
                     /inference="alignment:Splign:2.1.0"
     exon            498..592
                     /gene="calr3b"
                     /gene_synonym="calr3; calrl; id:ibd1087; wu:fc59g10"
                     /inference="alignment:Splign:2.1.0"
     exon            593..802
                     /gene="calr3b"
                     /gene_synonym="calr3; calrl; id:ibd1087; wu:fc59g10"
                     /inference="alignment:Splign:2.1.0"
     exon            803..916
                     /gene="calr3b"
                     /gene_synonym="calr3; calrl; id:ibd1087; wu:fc59g10"
                     /inference="alignment:Splign:2.1.0"
     exon            917..1060
                     /gene="calr3b"
                     /gene_synonym="calr3; calrl; id:ibd1087; wu:fc59g10"
                     /inference="alignment:Splign:2.1.0"
     exon            1061..1153
                     /gene="calr3b"
                     /gene_synonym="calr3; calrl; id:ibd1087; wu:fc59g10"
                     /inference="alignment:Splign:2.1.0"
     exon            1154..2917
                     /gene="calr3b"
                     /gene_synonym="calr3; calrl; id:ibd1087; wu:fc59g10"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      

ccacgcgcttttcccgagcaactgaggaaccgagggcagcagagcgcgagagcgagcgtgcgggagtcatttctttaaaaaaaattaattaacgaagatgcaaatttcattattacagttaatttcggctgcattattggcactggcagccaatgcagaagtgtacttaaacgagcagtttctggacggagatgcctggaggagtcgatgggttaattcggagcacaaatctgactacgggcagtttaaactgaccgctggaaacttctacggagacgctgagaaagataagggcctgcagaccagtcaagatgctcgtttctacgccacctctgtccgcttcgagccctttagcaatgagggcaaaacactggtgatccagtttacggttaaacacgagcagaagatcgactgtggaggcggatacgttaaagtcttcccagctgatctcaaccaggcggacatgcacggcgactcccagtattacatcatgtttgggcctgatatctgtggctacagcactaaaaaggttcacgtcatttttaactataagggacagaaccacctcatcaagaaagatatcaaatgcaaggatgatgaactgactcacctgtacacattgatcttgaatccggatcagacatatgaggttaagattgataatgagaaggtggagtccggctctctggaggaggactgggatttcttgcccccgaagaagatcaaggaccccgaagctaaaaaacccgaggactgggacgaccgggccaagattgatgacgagactgacaccaaacctgaggactgggataagcccgagaatatccccgatcctgatgctaagaagccagaagactgggatgaggacatggatggagagtgggagcctgccatgatccctaaccccgagtacaagggtgagtggaaacccaaacagattgacaacccgaactacaagggcgcctgggtgcatcctgaaatcgataacccagaacacgttcctgatgaccagatctacaaattcgacaatattggagtccttgggctggatctctggcaggtgaagtctggcaccatttttgacaacttcttcatctcagatgatgtgaaagaagctgaggaatttggaaatgaaacgtggggcgctacaaagggccccgagaagaaaatgaaggaggaacaggaagagaagaaacgcaaagaggaagaggaaaagaacaaggagcagaacaccgaagcagcagatgaggaagaggaagacgaaggagaggaggaagaggaagaagacgagactgaagagcctcaggaggacgaaggagacgaggatgtgcttcagaaagacgaactgtaaagcagaaatccatttttgtccatattccacattataccagggacgcgactgcagtgcattcattcaaaacaacacattcagactgaatgggaaccaatggaggttctcagaccacgcgttcaatcagtgagctttgacccctaaatagccatttaaagagacggttcacccaaaaactgaaacttgtcattatttattcatcctccatttgttccaaaccaacgtttctttcctctgttgaacacaaaataagatattctgtgttatattttattgtaattagtagcatagtattttttttatgctactatggaagtcaatgggtcccagcaaccagcattctacaaaatatcttcctttgtgttcaactgaagacagaaactcttaaacgtttagatttttgggtgaactgtccccctaaataccttattttgtttgtttatttagaagatctcagcccatgcatttaattagtgatctttgacccctaaatggccagttaaaaagacagttaacctaaaaaatttaattctctctttcatttattcatcaatgacttgttccaaaccggtttgagtttctttcttctgttgaacacacaataagatatgctgaagaatactgtattttattatgattggtaatatgaattagtagcaaaactatggaagtcaatgggtcccagaaaccagcattctttaaactattttcctttgtgttcaaccgagtaaagaaactcaaagtttagaaccacttgagtgagtggtgattttgggtgaactgtccccttaaataccttgtttttgtttgtttgtttgtttgtttgtttggtagttttcagctcatgcattacatagttagtgacatttgacccctaaatagccagttaaagagaccgttcagccaaaaaaaaaaaaatgaagtccttcgcttgtttcaaacctgttcgagtttctttcttctgttgaacacaaaggaagatattctgaagaatgctggtaaaaacaaacattgacttgcattgcattttttttgctcctcctatggatgttaattctttgagcattctttgaagtgtcttttttttgtgtttttatagaacaaaaaaaataagtttagaatcatttgaattggtaaatgttcttttttaatgtgaattgtccctttaaatatcatcttttatttgtttgtttgtttttgagtgtttctttatggtaccggggtccgacatgtgaaacacctttaaagaactggtctatgatcagtttattgtgccttttgtgtatgcaaaattgttgtttctgttagtttgaaggaattggattaagttagattaacagtctgtatcaggaagccggattagctttataggcagttatgttttagtttagtttgcaccaattctgcattttgtagctcagcttctacataataccatggcacataatgactttacctgtttgttcaaactacttatttatgacgagtcgaaccaacacagtttttaactttttttttttttttttggccaacttaattgttttatgttcattcaacttaaattta

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