LOCUS       NM_001423197            3683 bp    mRNA    linear   VRT 06-JUL-2023
DEFINITION  Danio rerio argonaute RISC catalytic component 3a (ago3a), mRNA.
ACCESSION   NM_001423197
VERSION     NM_001423197.1
KEYWORDS    RefSeq.
SOURCE      Danio rerio (zebrafish)
  ORGANISM  Danio rerio
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Ostariophysi;
            Cypriniformes; Danionidae; Danioninae; Danio.
REFERENCE   1  (bases 1 to 3683)
  AUTHORS   Raman R, Ryon M and Jagadeeswaran P.
  TITLE     RNaseH-mediated simultaneous piggyback knockdown of multiple genes
            in adult zebrafish
  JOURNAL   Sci Rep 10 (1), 20187 (2020)
   PUBMED   33214638
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3683)
  AUTHORS   Bayes A, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A,
            Choudhary JS, Emes RD and Grant SG.
  TITLE     Evolution of complexity in the zebrafish synapse proteome
  JOURNAL   Nat Commun 8, 14613 (2017)
   PUBMED   28252024
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3683)
  AUTHORS   Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M,
            Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD,
            Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov
            GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W and
            Hertzano R.
  TITLE     RFX transcription factors are essential for hearing in mice
  JOURNAL   Nat Commun 6, 8549 (2015)
   PUBMED   26469318
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3683)
  AUTHORS   McFarlane L, Svingen T, Braasch I, Koopman P, Schartl M and Wilhelm
            D.
  TITLE     Expansion of the Ago gene family in the teleost clade
  JOURNAL   Dev Genes Evol 221 (2), 95-104 (2011)
   PUBMED   21556854
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CR762469.15, CABZ01034603.1, CABZ01034602.1, CABZ01034601.1 and
            CABZ01034600.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: JX448393.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA2168446,
                                           SAMEA2168447 [ECO:0006172]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-708               CR762469.15        14797-15504         c
            709-880             CABZ01034603.1     3865-4036           c
            881-1001            CABZ01034603.1     1321-1441           c
            1002-1210           CABZ01034603.1     283-491             c
            1211-1347           CABZ01034602.1     13900-14036         c
            1348-1482           CABZ01034602.1     11070-11204         c
            1483-1570           CABZ01034602.1     8445-8532           c
            1571-1718           CABZ01034602.1     6277-6424           c
            1719-1838           CABZ01034602.1     4551-4670           c
            1839-1982           CABZ01034602.1     1862-2005           c
            1983-2116           CABZ01034601.1     1048-1181           c
            2117-2301           CABZ01034600.1     10659-10843         c
            2302-2461           CABZ01034600.1     7143-7302           c
            2462-2552           CABZ01034600.1     5560-5650           c
            2553-2747           CABZ01034600.1     5137-5331           c
            2748-2882           CABZ01034600.1     4924-5058           c
            2883-2984           CABZ01034600.1     3348-3449           c
            2985-3184           CABZ01034600.1     1466-1665           c
            3185-3683           CABZ01034600.1     647-1145            c
FEATURES             Location/Qualifiers
     source          1..3683
                     /organism="Danio rerio"
                     /mol_type="mRNA"
                     /strain="Tuebingen"
                     /db_xref="taxon:7955"
                     /chromosome="16"
                     /map="16"
     gene            1..3683
                     /gene="ago3a"
                     /gene_synonym="eif2c1; eif2c3a; sb:eu275; sb:eu416"
                     /note="argonaute RISC catalytic component 3a"
                     /db_xref="GeneID:567622"
                     /db_xref="ZFIN:ZDB-GENE-051107-16"
     exon            1..708
                     /gene="ago3a"
                     /gene_synonym="eif2c1; eif2c3a; sb:eu275; sb:eu416"
                     /inference="alignment:Splign:2.1.0"
     misc_feature    687..689
                     /gene="ago3a"
                     /gene_synonym="eif2c1; eif2c3a; sb:eu275; sb:eu416"
                     /note="upstream in-frame stop codon"
     CDS             690..3293
                     /gene="ago3a"
                     /gene_synonym="eif2c1; eif2c3a; sb:eu275; sb:eu416"
                     /EC_number="3.1.26.n2"
                     /note="eukaryotic translation initiation factor 2C, 1;
                     argonaute 3a; eukaryotic translation initiation factor 2C,
                     3a"
                     /codon_start=1
                     /product="protein argonaute-3"
                     /protein_id="NP_001410126.1"
                     /db_xref="GeneID:567622"
                     /db_xref="ZFIN:ZDB-GENE-051107-16"
                     /translation="

MEIGTTGAVGAQSLFSMPRRPGYGTMGKPIKLLANCFQVEIPKMDVYLYEVDIKPEKCPRRVNREVVDSMVQHFKVTIFGDRRPVYDGKKSLYTANPLPVAPAGVDLDVTLPGEGGKDRPFKVSIKFVSLVSWHLLHEVLTGRSMPEPLELDKPISTNPVHAVDVVLRHLPSMKYTPVGRSFFSAPEGYDHPLGGGREVWFGFHQSVRPAMWKMMLNIDVSATAFYKAQPVIQFMCEVLDIHNIDEQPRPLTDSHRVKFTKEIKGLKVEVTHCGTMRRKYRVCNVTRRPASHQTFPLQLENGQTVERTVAQYFREKYNLQLKYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIKATARSAPDRQEEISRLVRSANYEADPFVQEFQFKVRDEMAHVTGRVLPAPMLQYGGRVSTEHFMNRTVATPSHGVWDMRGKQFHTGVEIKMWAIACFATQRQCREEILKGFTDQLRKISKDAGMPIQGQPCFCKYAQGADSVEPMFRHLKNTYSGLQLIIVILPGKTPVYAEVKRVGDTLLGMATQCVQVKNVVKTSPQTLSNLCLKINVKLGGINNILVPHQRPSVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDAHPSRYCATVRVQRPRQEVIQDLSSMVRELLIQFYKSTRYKPTRIIFYRDGVSEGQFRQVLYYELLAIREACISLEKEYQPGITYIVVQKRHHTRLFCADRNERVGRSGNIPAGTTVDTDITHPYEFDFYLCSHAGIQGTSRPSHYHVLWDDNCFTADEFQLLTYQLCHTYVRCTRSVSIPAPAYYAHLVAFRARYHLVDKEHDSAEGSHVSGQSNGRDPQALAKAVQIHHDTLRTMYFA"

     misc_feature    906..1190
                     /gene="ago3a"
                     /gene_synonym="eif2c1; eif2c3a; sb:eu275; sb:eu416"
                     /note="N-terminal domain of argonaute; Region: ArgoN;
                     pfam16486"
                     /db_xref="CDD:465134"
     misc_feature    1218..1370
                     /gene="ago3a"
                     /gene_synonym="eif2c1; eif2c3a; sb:eu275; sb:eu416"
                     /note="Argonaute linker 1 domain; Region: ArgoL1;
                     pfam08699"
                     /db_xref="CDD:462567"
     misc_feature    1371..1733
                     /gene="ago3a"
                     /gene_synonym="eif2c1; eif2c3a; sb:eu275; sb:eu416"
                     /note="PAZ domain, argonaute_like subfamily. Argonaute is
                     part of the RNA-induced silencing complex (RISC), and is
                     an endonuclease that plays a key role in the RNA
                     interference pathway. The PAZ domain has been named after
                     the proteins Piwi,Argonaut, and Zwille; Region:
                     PAZ_argonaute_like; cd02846"
                     /db_xref="CDD:239212"
     misc_feature    order(1527..1529,1572..1574,1614..1616,1626..1628,
                     1680..1682,1701..1703,1707..1709)
                     /gene="ago3a"
                     /gene_synonym="eif2c1; eif2c3a; sb:eu275; sb:eu416"
                     /note="nucleic acid-binding interface [nucleotide
                     binding]; other site"
                     /db_xref="CDD:239212"
     misc_feature    1866..3164
                     /gene="ago3a"
                     /gene_synonym="eif2c1; eif2c3a; sb:eu275; sb:eu416"
                     /note="PIWI domain, Argonaute-like subfamily. Argonaute is
                     the central component of the RNA-induced silencing complex
                     (RISC) and related complexes. The PIWI domain is the
                     C-terminal portion of Argonaute and consists of two
                     subdomains, one of which provides the...; Region:
                     Piwi_ago-like; cd04657"
                     /db_xref="CDD:240015"
     misc_feature    order(2298..2300,2310..2312,2346..2357,2364..2366,
                     2388..2390,2397..2399,2409..2411,2421..2423)
                     /gene="ago3a"
                     /gene_synonym="eif2c1; eif2c3a; sb:eu275; sb:eu416"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240015"
     misc_feature    order(2502..2504,2508..2510,2718..2720,3132..3134)
                     /gene="ago3a"
                     /gene_synonym="eif2c1; eif2c3a; sb:eu275; sb:eu416"
                     /note="active site"
                     /db_xref="CDD:240015"
     exon            709..880
                     /gene="ago3a"
                     /gene_synonym="eif2c1; eif2c3a; sb:eu275; sb:eu416"
                     /inference="alignment:Splign:2.1.0"
     exon            881..1001
                     /gene="ago3a"
                     /gene_synonym="eif2c1; eif2c3a; sb:eu275; sb:eu416"
                     /inference="alignment:Splign:2.1.0"
     exon            1002..1210
                     /gene="ago3a"
                     /gene_synonym="eif2c1; eif2c3a; sb:eu275; sb:eu416"
                     /inference="alignment:Splign:2.1.0"
     exon            1211..1347
                     /gene="ago3a"
                     /gene_synonym="eif2c1; eif2c3a; sb:eu275; sb:eu416"
                     /inference="alignment:Splign:2.1.0"
     exon            1348..1482
                     /gene="ago3a"
                     /gene_synonym="eif2c1; eif2c3a; sb:eu275; sb:eu416"
                     /inference="alignment:Splign:2.1.0"
     exon            1483..1570
                     /gene="ago3a"
                     /gene_synonym="eif2c1; eif2c3a; sb:eu275; sb:eu416"
                     /inference="alignment:Splign:2.1.0"
     exon            1571..1718
                     /gene="ago3a"
                     /gene_synonym="eif2c1; eif2c3a; sb:eu275; sb:eu416"
                     /inference="alignment:Splign:2.1.0"
     exon            1719..1838
                     /gene="ago3a"
                     /gene_synonym="eif2c1; eif2c3a; sb:eu275; sb:eu416"
                     /inference="alignment:Splign:2.1.0"
     exon            1839..1982
                     /gene="ago3a"
                     /gene_synonym="eif2c1; eif2c3a; sb:eu275; sb:eu416"
                     /inference="alignment:Splign:2.1.0"
     exon            1983..2116
                     /gene="ago3a"
                     /gene_synonym="eif2c1; eif2c3a; sb:eu275; sb:eu416"
                     /inference="alignment:Splign:2.1.0"
     exon            2117..2301
                     /gene="ago3a"
                     /gene_synonym="eif2c1; eif2c3a; sb:eu275; sb:eu416"
                     /inference="alignment:Splign:2.1.0"
     exon            2302..2461
                     /gene="ago3a"
                     /gene_synonym="eif2c1; eif2c3a; sb:eu275; sb:eu416"
                     /inference="alignment:Splign:2.1.0"
     exon            2462..2552
                     /gene="ago3a"
                     /gene_synonym="eif2c1; eif2c3a; sb:eu275; sb:eu416"
                     /inference="alignment:Splign:2.1.0"
     exon            2553..2747
                     /gene="ago3a"
                     /gene_synonym="eif2c1; eif2c3a; sb:eu275; sb:eu416"
                     /inference="alignment:Splign:2.1.0"
     exon            2748..2882
                     /gene="ago3a"
                     /gene_synonym="eif2c1; eif2c3a; sb:eu275; sb:eu416"
                     /inference="alignment:Splign:2.1.0"
     exon            2883..2984
                     /gene="ago3a"
                     /gene_synonym="eif2c1; eif2c3a; sb:eu275; sb:eu416"
                     /inference="alignment:Splign:2.1.0"
     exon            2985..3184
                     /gene="ago3a"
                     /gene_synonym="eif2c1; eif2c3a; sb:eu275; sb:eu416"
                     /inference="alignment:Splign:2.1.0"
     exon            3185..3683
                     /gene="ago3a"
                     /gene_synonym="eif2c1; eif2c3a; sb:eu275; sb:eu416"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      

ccatatttagctgcagcgagtctcgcagaggctctgagtgtttgagaaagcgggcagagagagagttctgcttccaacttactttatgtgaccactggcggaaaaccacgggaatacgcgcttataaaaagccactagagccgagagcgaaattcatggcagaccggggactttaagcgaagacacgactggatttttgtttcctctcagatgggaatgtagcttagctggttagcctggccactagcagtagcaagttagctctgtcgaagagcggaggcgacataagtggacttttacttgctataacgtcataacacctctacaaaccgccctagtctttccaaagacgtgtttctgaaggcgtcgccgtcacagaaacagagactcgggactgaatttgggctgactgagaggaacttaccgttaccgttttagctagctggctagctggccgtcacgtcaatctgtcaaccgtacccgaaatcccgaccggatagtttgagtcacataccgggcaaccaaacattatctccggtgacacacgagcctcagggaaggtggactaataccgagaggaatcgcttgcggtgggttaacgcaaccggtttgtgcggccataggacagtcagagtttctgaagttcatcaactgcgagccggggacggaaccggacagagacctcatgaatggaaatcggaactacaggagccgttggggcccagtccctgttctccatgccacggaggcccggctatggcaccatggggaaacctatcaaactgctggccaactgcttccaggtggagatcccaaagatggacgtctacctgtacgaggtggacatcaagcccgagaagtgtccacggagagtaaatagggaggtggtggactccatggtgcagcactttaaagtcaccatctttggggatcgaaggccagtttatgatgggaagaaaagtctctacaccgccaacccacttcctgtggcacctgcaggggtggatctggatgtcacgctgccaggtgaagggggcaaagaccggccctttaaagtctcaattaagtttgtgtctctggtgagctggcacctgctgcacgaggtgctaaccgggcgcagtatgccagagccgctggaactggacaagccaatcagcaccaacccagtgcacgctgtggatgtggtgctccgccatctgccctccatgaagtacacacccgtgggccgctcatttttctccgccccggagggttatgatcatcctctaggtggaggcagagaggtgtggttcggtttccatcagtctgttcggcccgccatgtggaaaatgatgctcaacattgatgtgtcagccacggctttctacaaagcacagccagtcattcagttcatgtgtgaagtcctggacatccacaacatcgacgagcagccgcgacccctcacagactcccacagagtcaaattcaccaaagaaatcaaaggtctgaaggtggaggtgacccactgcgggaccatgcggaggaagtaccgcgtctgcaatgtgacccgccggcctgccagccaccaaacgttccctttgcagttggagaacggccaaactgtagaacggacagtagctcagtatttcagagagaagtacaacctgcagctcaagtaccctcacctgccctgtctacaggtgggacaggagcagaaacacacatacctgcctctggaggtttgcaatattgtagcagggcagcggtgcatcaagaaactgacagataatcagacgtccaccatgatcaaagccacggcgcgctccgcaccagaccgacaggaggagatcagcagactggtgcgcagcgctaactatgaagccgacccgttcgtgcaggagtttcagtttaaggtccgggatgagatggcccacgtgacggggcgcgtgttgcccgcgcccatgctgcagtacgggggcagggtgagcacagagcactttatgaaccgcactgtcgccacaccgagtcacggcgtttgggacatgagaggaaagcagtttcacaccggcgtggagatcaagatgtgggccatcgcctgcttcgccacacagaggcagtgcagagaggagatcctcaagggttttaccgaccagctgcgcaaaatctcgaaggatgctgggatgccgattcagggtcagccgtgtttctgtaagtacgctcagggggccgacagcgtggagcccatgttcagacacctgaagaacacttactctggactgcagctcatcatcgtcatcctgcctggaaaaacccccgtctacgcggaagtgaagcgtgttggagacactcttctaggaatggccactcagtgtgtgcaggtgaagaacgtggtaaaaacctccccgcagacgctctccaacctctgcctgaaaatcaacgtcaaactcggcggcatcaacaacatcctcgtgccgcatcaacggccatcagttttccagcagccagtcatctttctgggggcggacgttacacatccccctgcaggagatgggaagaaaccgtccatcgcagcggtggtgggcagtatggacgctcatcccagcaggtattgtgccactgtgcgcgtccagagaccccgtcaggaggtcattcaggatttgtcctcaatggtgcgagagttactcatccagttctacaaatccactcgctacaagcccaccagaatcatcttctacagggacggtgtttctgagggccagttcagacaggtgttgtattatgagctgctggccattcgagaggcctgtatcagtctggagaaggagtaccagcccggcatcacgtatatagtggtgcagaaacgccaccacacacgcctcttctgtgccgaccgcaacgagagggtgggtcgcagtggaaacattcctgctggtacgacggtggatacagatatcacacacccttatgagtttgacttttacctctgcagtcatgctggaatacagggcaccagcaggccctcccactatcatgtcctgtgggacgacaactgcttcactgctgatgagttccagctgctcacctaccagctgtgccacacgtatgtgcgctgcacccgctccgtctccatccccgcgccagcctactacgcccacctggtggccttccgcgcccgataccacttggtggacaaagaacacgacagtgccgagggaagccatgtctccggacagagcaacggccgggacccccaagcgctggccaaagctgtgcagattcaccacgacaccctgaggaccatgtactttgcctaagccagctcacgttcatctagtcatgcacacaaacccgacaaccacacacacacacacatgctgacaaacacacacacctcccagaatcctgtgcgtcctgaagggtcgatctctgtggcgattgaagcacggcccacccgacactgtgcagcaggacgaggaaggcctttaaggaagccgacctgctttaaaaccacacatggacctcggcgctattatgcaatattaaaccagccaactgaggccgttctgtcagatccccctccaaaaccaaaagggaagtccgtctgtatatccctcccgctccttttcctgtctatagacgtcacgttttaatcacttccccccccctgcctgtcacgccttgtacctcaacgtcttttctaacag

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