LOCUS       XM_077414732            5298 bp    mRNA    linear   INV 30-SEP-2025
DEFINITION  PREDICTED: Temnothorax americanus copper-transporting ATPase 1
            (Atp7), transcript variant X2, mRNA.
ACCESSION   XM_077414732
VERSION     XM_077414732.1
DBLINK      BioProject: PRJNA1327953
KEYWORDS    RefSeq.
SOURCE      Temnothorax americanus
  ORGANISM  Temnothorax americanus
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Formicoidea; Formicidae; Myrmicinae; Temnothorax.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027509515) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_048541705.1-RS_2025_09
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 09/24/2025
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5298
                     /organism="Temnothorax americanus"
                     /mol_type="mRNA"
                     /isolate="GAGA-0224"
                     /isolation_source="Broadleaf forest"
                     /db_xref="taxon:1964332"
                     /chromosome="Unknown"
                     /tissue_type="pupae pool"
                     /geo_loc_name="USA"
                     /collection_date="2016-06-30"
                     /collected_by="Susanne Foitzik"
     gene            1..5298
                     /gene="Atp7"
                     /note="copper-transporting ATPase 1; Derived by automated
                     computational analysis using gene prediction method:
                     Gnomon. Supporting evidence includes similarity to: 9
                     Proteins, and 100% coverage of the annotated genomic
                     feature by RNAseq alignments, including 105 samples with
                     support for all annotated introns"
                     /db_xref="GeneID:143902033"
     CDS             443..4333
                     /gene="Atp7"
                     /codon_start=1
                     /product="copper-transporting ATPase 1 isoform X2"
                     /protein_id="XP_077270858.1"
                     /db_xref="GeneID:143902033"
                     /translation="

MEAAKVMEPQNGADPSRAQRDEEKGSDGRGVKPKRRKSDGDDPDSKGITHLVRALRTSEAWQLAQETSTVKIGIEGMTCQSCANNIERVIGNRPDVVGIKVVLEEKAGYIEYKTHEITPRELAEAIEDMGYTASLPSNLTSGNAENKANNSSIPATGTCNIHVDGMTCQSCVKNITDVLSEKPGIKNVNVSLEDKEAKVSYSSGDVTVDQIVMYIEDMGFTAYVKIAKDETSQLDADINKKKKKTELVLQANGAGDVKKQLSKCFLRITGMTHAHHVTGIEKHCKKLYGVNSILVALMAAKAEVTYNPDKIKAADIASCISELGFPTTLIEEFGTEEVEVELKIMGMTCASCVNKIESTVKKLPGVRSAMVALATQRGKFKYDVEMTGVRDIIECINNLGIYTASLFSNRDKENRDYLDQKEEISKWRRTFFISLIFGVPSMVSMTYFMLVMSFGNKTHEEMCCIVPGLSWENLLLFVFSTPVQFFGGWYFYVQAYKALRHGTTNMDVLISMTTTISYLYSIAVLTAAMIMQENVSPQTFFDTPPMLLVFISLGRWLEHVAKGKTSEALSKLLSLKATDAVLVSLGHHKEILTERSISVDMVQRGDILKVVPGAKVPVDGRVLFGESTCDESLITGESMPVQKKEGSMVIGGSINENGSLVITATHTGEHTTLAQIVRLVEEAQTNKAPIQQLADKIAGYFIPLVIVVSLVTLVIWIIIGYMNVEQLPMSHNDQFNKHGLSRVEIIFQYAFRSALAVLAIACPCALGLATPTAVMVGTGVGALNGILIKGAEPLENAHKVKCVVFDKTGTLTHGAPTVTKIELFVEEMVCSLGTLLTVVGTAERNSEHPIAAAIVRYVQDTIGSKTGQCTNFEAVPGCGLKCKVSHLEGALGESLQSENLINYMDQSHNVSTGTYNVNNVRIDKLSIVEKPVTDRQEMQKLNLQLLLEPLRRDSNMIDEIYEMCIGNREWMRRNAINVPQEVDERMVNEENLGHTTVLVAINNLLVAMISVADTVKPEAHLAVYTLKKMGLEVILLTGDNKKTAASIAKQVGIDRVFAEMLPSHKVAKIQQLQKQGLRVAMVGDGVNDSPALAQSDVGIAIASGTDVAVEAADVVLMRNDLLDVIACLDLSRKTVRRIRLNFLFASVYNLLGIPIAAGVFSSFGFFLQPWMSSAAMALSSVSVVGSSLLLKLYRKPTKATLETPGYLEVRFTHSIGRSNDLNTIIVDPFHRGSNDSLNRYKSAMNRSTSTLSRIFGKSKLEVEGHLLGEEADETDLAVDFSNYRKNAKNSMDITSV"

     misc_feature    662..844
                     /gene="Atp7"
                     /note="Heavy-metal-associated domain (HMA) is a conserved
                     domain of approximately 30 amino acid residues found in a
                     number of proteins that transport or detoxify heavy
                     metals, for example, the CPx-type heavy metal ATPases and
                     copper chaperones. HMA domain...; Region: HMA; cd00371"
                     /db_xref="CDD:238219"
     misc_feature    order(671..679,686..688)
                     /gene="Atp7"
                     /note="metal-binding site [ion binding]"
                     /db_xref="CDD:238219"
     misc_feature    923..1108
                     /gene="Atp7"
                     /note="Copper chaperone CopZ [Inorganic ion transport and
                     metabolism]; Region: CopZ; COG2608"
                     /db_xref="CDD:442020"
     misc_feature    1238..1435
                     /gene="Atp7"
                     /note="Copper chaperone CopZ [Inorganic ion transport and
                     metabolism]; Region: CopZ; COG2608"
                     /db_xref="CDD:442020"
     misc_feature    1463..1657
                     /gene="Atp7"
                     /note="Heavy-metal-associated domain (HMA) is a conserved
                     domain of approximately 30 amino acid residues found in a
                     number of proteins that transport or detoxify heavy
                     metals, for example, the CPx-type heavy metal ATPases and
                     copper chaperones. HMA domain...; Region: HMA; cd00371"
                     /db_xref="CDD:238219"
     misc_feature    order(1481..1489,1496..1498)
                     /gene="Atp7"
                     /note="metal-binding site [ion binding]"
                     /db_xref="CDD:238219"
     misc_feature    1727..3973
                     /gene="Atp7"
                     /note="P-type heavy metal-transporting ATPase, similar to
                     human copper-transporting ATPases, ATP7A and ATP7B;
                     Region: P-type_ATPase_Cu-like; cd02094"
                     /db_xref="CDD:319783"
     misc_feature    order(2726..2728,2732..2734,3884..3886)
                     /gene="Atp7"
                     /note="putative Cu binding site [ion binding]; other site"
                     /db_xref="CDD:319783"
     misc_feature    order(2858..2866,3065..3067,3335..3343,3431..3433,
                     3551..3559,3617..3619,3626..3628,3635..3637,3692..3694,
                     3701..3703)
                     /gene="Atp7"
                     /note="putative ATP binding site [chemical binding]; other
                     site"
                     /db_xref="CDD:319783"
     polyA_site      5298
                     /gene="Atp7"
                     /experiment="COORDINATES: polyA evidence [ECO:0006239]"
ORIGIN      

aacttgcgtgccgtgcgtacccgttgttggctgttatccgtctccgtctccgtctccgtctccggtgcgtgctccccgtcctcgcggccgattaatccgcccgtcgacgcgggatagtcgcgctcgcgctcggcgagcgacgcgctcatcccaactcccgaggagggctctccgttgaccgttcacggagagcgcgaggcgacacatttaggttataaaccgcgcgaggtgccgactgccgcgggaatcgaacggtggagaccgggtggacctgttcgttgtgcgttgtgcgttgtgcgtgctgcgtgcacaactctcctccacgagccgcgcgtcgtatgcggatccttgccgatgtgtgcgcgcgggcgctgaagggacgagcagtcagttggcatgattctctctaacgcgcccgaacctcgtctgattacggagaagaatggaagcggcgaaggtgatggagcctcagaacggtgctgacccttcacgggcacaaagggatgaagagaaaggaagtgatggtagaggtgtcaagcccaaacgtcgtaaaagtgatggcgacgaccccgattctaagggtatcacgcacctggtgcgtgcactacgcacgtcggaagcgtggcagctcgcgcaggagaccagcacggttaaaatcggcattgagggtatgacctgtcagagttgcgcgaacaacatcgaaagggtgatcggcaatcggcccgacgtcgtcggtatcaaagtggttctggaggagaaagccggctacatcgagtacaaaacgcacgagatcacaccccgtgaattggcagaggcgatcgaggacatggggtacaccgccagcctgccatccaacctgacatccggcaacgcggagaacaaagcgaacaactcttcaatacctgccaccggcacttgcaacatacacgtagacggaatgacgtgtcagtcgtgcgttaagaacattaccgatgttctctccgaaaagcccggtataaagaacgtgaacgtcagcctggaggataaagaagccaaagtctcttacagtagcggtgatgtaacagtcgatcagatagtaatgtatatagaggacatgggcttcactgcgtatgtaaaaatagcgaaggacgagacatcgcaattggatgctgatatcaataaaaagaagaagaagacggagctggtgctgcaagcgaacggcgctggtgatgtcaaaaaacaattgtcaaagtgcttcttgcgtataactggtatgactcatgctcaccatgtcactggaatagagaaacattgtaaaaagctgtacggagtaaatagcatactggttgctttgatggccgccaaggcagaagttacatacaatcctgacaaaataaaagcggcggatattgcttcctgtatatcggaattgggttttcctacgactttaatcgaggaatttgggaccgaagaagtcgaagtcgagttaaaaatcatgggaatgacgtgcgcgtcttgcgtaaataaaatagaatcgaccgtgaagaagctacctggtgtacgatcggcaatggtcgctctagcgacgcagcggggcaagttcaagtacgacgtggaaatgactggtgtcagagatatcatcgaatgtatcaataatttgggtatttacacggccagtctgtttagcaatcgagataaggaaaatagggactacctggatcaaaaagaagagataagtaaatggcgaaggacgttcttcatatcgctgattttcggagttccgagtatggtatcgatgacatatttcatgctagttatgtcgttcggtaacaagacacacgaggaaatgtgttgcatagtacctggtctttcttgggaaaatctgttactcttcgtgttctccacgccggtgcagttttttggtggctggtatttctatgtgcaagcctataaagctttgagacatggtacaaccaacatggacgttttaatctcgatgactactacgatctcatatctttattctatcgccgttcttactgctgctatgataatgcaagagaacgtcagtccacagacgttcttcgacactccaccgatgctcttggtcttcatcagtttgggaagatggctcgagcacgtcgcgaagggaaagacgtccgaagctctctcaaaactgttatctttaaaagccacggatgcagtgttggtttcgctaggacaccacaaagaaatattaacggaacgctcgataagcgttgacatggtgcaacgtggcgacatactaaaagtggtcccaggtgccaaagtgcctgttgacggccgcgtattgttcggtgagtcgacctgcgacgagagtctgatcaccggtgagagtatgccagtgcaaaagaaggaaggctcgatggtgatcggcggttcgatcaatgagaacggatcgttggtgatcaccgccacgcacaccggcgagcacacgacgctggcgcagatcgttcgcttggtggaggaggcgcagacgaacaaggcacctatccagcaactggccgacaagatagccggctattttatacctctggtcatagtggtctccctagtgacgctagtgatttggatcatcatcggctacatgaatgtcgagcagctgcccatgtcgcacaacgaccagttcaataagcacggcttgagtcgggtggagatcatatttcaatacgcctttcgaagtgccctggcagtactcgcgattgcttgtccctgcgctcttggcctggccaccccgaccgcggtcatggtcggcacgggcgtaggtgcactaaacggcattctgatcaagggcgccgagccgctggagaacgcgcacaaagtcaaatgcgttgtcttcgacaagacgggaacgttgacgcacggtgctccaactgttaccaagatcgaattgttcgtggaagagatggtctgttcattggggacgttgctgacggtcgtcggcacggcagaaagaaacagcgaacatcctatcgcggcagcgatcgtacgctacgtgcaggacacaatcggatcgaaaactggacaatgtacgaattttgaagcagttcccggctgcggtttgaaatgtaaggtatctcatttggaaggtgcgctgggcgagtcgttgcaatccgaaaaccttataaactacatggatcagtcgcacaacgtgtcgactggaacgtacaatgtcaataacgtgcgcatcgacaagttgtcgatcgtcgagaagccggtaaccgatcgtcaagagatgcaaaagttaaatctacaattacttttggagcctctgcgtcgcgattcgaacatgattgacgagatatacgagatgtgcatcggtaatcgggaatggatgcgccggaatgcgatcaacgttccgcaggaagtcgacgagaggatggtcaacgaggagaacctaggacacaccacggttttggtcgcgataaacaatcttctggtcgccatgatcagtgtcgctgatacagtaaagcccgaggcacatctcgcagtctataccctgaagaaaatgggattggaggtgatcctcttgacgggcgataataagaaaaccgcggcttcgattgccaaacaggtcggcatcgacagagttttcgcggagatgttaccgtcccacaaggtggctaaaattcagcaactgcaaaaacagggtttgagagtcgccatggtgggagatggtgtcaatgacagtccggctctagctcaatcagatgtcggtatcgctatcgcgtctggcacagatgttgccgtggaggctgccgacgtggttttgatgcgcaacgatcttctggatgtaatcgcgtgtttagatctatcgaggaaaacggtccgcaggataagactgaatttcttattcgctagtgtctacaatcttctgggtattcccatagctgccggagtatttagctcctttggtttcttcctacagccgtggatgtcttccgccgccatggctttaagtagcgtatcggtagtaggaagttccttattattgaagctttatcgtaaaccgactaaagccacgttggaaactcccggatacctagaagttaggtttacccattccatagggcgatcgaacgatttgaatacgataatagtggatccattccatcgtggatcaaacgattcgctgaacagatataaatcagctatgaacagatcaacgtccacactgtccagaatatttggaaagtcaaaactcgaggtggagggccatctccttggcgaggaagcagatgaaactgatttagccgtggatttttcgaattatcgtaaaaacgcgaaaaactctatggacataacttccgtgtgaatcttgcatgagcactgtctgacaagcatttcaattctgttgactttgcagcaaatgaatcgaacgttaccaataattttaatattactttataatggagctaacagaagaaattgtataaaaatagtcaactaacaattctattataatataaagtgattttcacttgtcacattaaaaatgagctgcatcgcgaaagtaataccaataattcatacaagacttcgtgaatcgatttgagtgatattcatgtttaaacgaattgattgttatatttttataaactttatatattaagatgtataaataacaccgatctacctgactgtgaatgttttcataacaggatagcagtacttcctttttattgcaattaatagatacaagttttatcaatagttttttatcttattacatgcagatgtacttaattcaaatagtaaatggaaaaaattacgttttacacatttttaaaaatacatgcaatattttatatacgttttatatattataagacattacgtatgtccttttttattttatagattttaaaacaaacaaaatacaaaactattttatataaaatgtataaaactaaaaatcacgagattttagaaaaattttaaaaatctttaaagtttatcgcactcatttttaaattatacattttatttttcgactgaattgcaacgattgaaagtaaaagaaacaaaaatcatgatctgttaagatatttattataaattatacatgaaatatcagaaatcttaagcgctgcaattgtaacttgcattaaataaaaagagatgcgaataacggtgttacttttaaacgtatattaagaaatttttatattactatttatattttctttcaaatacttagtaagacaccacaatgtgcgtctaatatttaagatacaagacaatgcgtatgtatatatatacacaaaattttttagcata

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