LOCUS       XM_072371189            5575 bp    mRNA    linear   VRT 21-MAR-2025
DEFINITION  PREDICTED: Leuresthes tenuis argonaute RISC component 4 (ago4),
            transcript variant X2, mRNA.
ACCESSION   XM_072371189
VERSION     XM_072371189.1
DBLINK      BioProject: PRJNA1238911
KEYWORDS    RefSeq.
SOURCE      Leuresthes tenuis (California grunion)
  ORGANISM  Leuresthes tenuis
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Ovalentaria; Atherinomorphae; Atheriniformes;
            Atherinopsidae; Atherinopsinae; Leuresthes.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027325372) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_036924035.1-RS_2025_03
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.3
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 03/20/2025
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5575
                     /organism="Leuresthes tenuis"
                     /mol_type="mRNA"
                     /db_xref="taxon:355514"
                     /chromosome="Unknown"
     gene            1..5575
                     /gene="ago4"
                     /note="argonaute RISC component 4; Derived by automated
                     computational analysis using gene prediction method:
                     Gnomon. Supporting evidence includes similarity to: 9
                     Proteins"
                     /db_xref="GeneID:140275673"
     CDS             182..2806
                     /gene="ago4"
                     /codon_start=1
                     /product="protein argonaute-4 isoform X2"
                     /protein_id="XP_072227290.1"
                     /db_xref="GeneID:140275673"
                     /translation="

MEALGPGPPAPTSLFQPPRRPGLGTVGKPIRLLANHFQVQIPKIDVYHYDIDIKPEKRPRRVNREVVDTMVRHFKMQIFGDRQPGYDGKRNMYTAHPLPIGRDRVDLEVTLPGEGKDQTFKVSLQWVSVVSLQMLLEALSGHLNEVPEDSVQALDVITRHLPSMRYTPVGRSFFSPPEGYYHPLGGGREVWFGFHQSVRPAMWNMMLNIDVSATAFYRAQPVIEFMCEVLDIQNINEQTKPLTDSQRVKFTKEIRGLKVEVTHCGQMKRKYRVCNVTRRPASHQTFPLQLENGQAMECTVAQYFKQKYNLQLKYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIKATARSAPDRQEEISRLVKSNSMVGGPDPYLKEFGIVVHNDMTEVTGRVLPAPMLQYGGRVSTDTGRDCGRNKTVATPNQGVWDMRGKQFYAGIEIKVWAVACFAPQKQCREDLLKSFTDQLRKISKDAGMPIQGQPCFCKYAQGADSVEPMFKHLKMSYVGLQLIVVILPGKTPVYAEVKRVGDTLLGMATQCVQVKNVVKTSPQTLSNLCLKINAKLGGINNVLVPHQRPSVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDGHPSRYCATVRVQTSRQDMSQEQLFSQEVIQDLTNMVRELLIQFYKSTRFKPTRIIYYRGGVSEGQMKQVAWPELIAIRKACISLEEDYRPGITYIVVQKRHHTRLFCSDKTERVGKSGNVPAGTTVDSTITHPSEFDFYLCSHAGIQGTSRPSHYHVLWDDNCFTADELQLLTYQLCHTYVRCTRSVSIPAPAYYARLVAFRARYHLVDKDHDSAEGSHVSGQSNGRDPQALAKAVQIHYDTQHTMYFA"

     misc_feature    263..652
                     /gene="ago4"
                     /note="N-terminal domain of argonaute; Region: ArgoN;
                     pfam16486"
                     /db_xref="CDD:465134"
     misc_feature    683..835
                     /gene="ago4"
                     /note="Argonaute linker 1 domain; Region: ArgoL1;
                     pfam08699"
                     /db_xref="CDD:462567"
     misc_feature    836..1198
                     /gene="ago4"
                     /note="PAZ domain, argonaute_like subfamily. Argonaute is
                     part of the RNA-induced silencing complex (RISC), and is
                     an endonuclease that plays a key role in the RNA
                     interference pathway. The PAZ domain has been named after
                     the proteins Piwi,Argonaut, and Zwille; Region:
                     PAZ_argonaute_like; cd02846"
                     /db_xref="CDD:239212"
     misc_feature    order(992..994,1037..1039,1079..1081,1091..1093,
                     1145..1147,1166..1168,1172..1174)
                     /gene="ago4"
                     /note="nucleic acid-binding interface [nucleotide
                     binding]; other site"
                     /db_xref="CDD:239212"
     misc_feature    1337..2677
                     /gene="ago4"
                     /note="PIWI domain, Argonaute-like subfamily. Argonaute is
                     the central component of the RNA-induced silencing complex
                     (RISC) and related complexes. The PIWI domain is the
                     C-terminal portion of Argonaute and consists of two
                     subdomains, one of which provides the...; Region:
                     Piwi_ago-like; cd04657"
                     /db_xref="CDD:240015"
     misc_feature    order(1781..1783,1793..1795,1829..1840,1847..1849,
                     1871..1873,1880..1882,1892..1894,1904..1906)
                     /gene="ago4"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240015"
     misc_feature    order(1985..1987,1991..1993,2231..2233,2645..2647)
                     /gene="ago4"
                     /note="active site"
                     /db_xref="CDD:240015"
     polyA_site      5575
                     /gene="ago4"
                     /experiment="COORDINATES: polyA evidence [ECO:0006239]"
ORIGIN      

catacaaatccctagaagcagcagcggcagctcggctcagtccaggcggcagccatccacgcagctatctttattccgttttcttaaattagcccctttaagcttcgcgataccgcccccaatttcagcgtcggtacggagccgccaggccgggacagagacggagagagaggcctcggccatggaagcgctcggacccggcccgcctgctcccacctctctgtttcagcctccacggcgtcccggcctcggcacggtggggaaacccatccggctccttgccaaccacttccaggtgcagattcccaagattgatgtctatcactatgacatcgacatcaagcctgagaaacggcctcgaagggtcaacagggaggtggtcgacaccatggtgcggcacttcaagatgcagatctttggagaccgacagcctggttatgatgggaagaggaacatgtacacagcacatccgctgccgatcgggagagaccgggtggatctggaggtgaccctgccaggtgaggggaaggatcagacgttcaaggtgtctctgcagtgggtgtctgtggtcagtctccagatgctgttggaagccctgtcgggtcacctgaacgaggtccccgaggactccgttcaggccctggacgtcatcacacgccaccttccctccatgaggtacactcctgtggggcgctctttcttctcccctccggagggatactaccatcctctgggtggaggccgggaggtgtggttcggcttccatcagtctgtccgtcctgccatgtggaacatgatgctgaacatcgacgtgtcggccactgctttctaccgcgctcagcctgtgatcgagttcatgtgcgaagtgctcgacattcagaacatcaacgagcagacgaaaccgctgacggactcgcagagggtcaaattcaccaaggaaataagaggtttgaaggtcgaggtcacgcactgtggacagatgaagaggaaatatcgcgtttgcaacgtcacgcggcgcccagccagccaccaaacgttcccgttgcagctggagaacggccaggccatggagtgcacagtcgctcagtatttcaagcagaagtacaatctgcagctcaagtatccacatctaccgtgtctgcaagtgggacaggagcagaagcacacctaccttcccctggaggtctgcaacatcgtggccggccagcgttgcatcaagaaactgacggacaaccagacgtccaccatgattaaagcgaccgctcgctccgcccccgacagacaggaggagatcagcagactggtcaaaagcaacagtatggtgggggggccggacccctacctgaaggagtttggcattgtggtgcacaatgacatgaccgaggtaacggggcgtgtcctcccagcgcccatgctgcagtatgggggccgggtgagtacagacacagggagggactgtggaaggaacaaaaccgtggccacgcccaaccagggcgtgtgggacatgcgtgggaagcagttctacgctggcatcgagatcaaggtgtgggccgtggcctgcttcgccccccagaaacagtgccgggaggacctgctgaagagtttcactgatcagctgaggaagatttcgaaggacgccgggatgcccatccagggtcagccgtgcttctgtaaatacgcccaaggagccgacagcgtggagcccatgttcaagcacctcaagatgtcgtacgtcggcctgcagctgatcgtggtcatcctgccgggaaaaacgccggtctacgctgaggtgaagcgggtgggagacacgctcctgggcatggccacccagtgcgtccaggtgaagaacgtggtgaagacgtcccctcagaccctgtccaacctctgcctcaagatcaacgccaagctgggaggcatcaacaacgtcctggtgcctcatcagaggccctcagtgttccagcagcccgtcatcttcctcggcgccgacgtgacgcaccctcctgctggcgatggcaagaagccgtccatcgcggcggtggtgggcagcatggacgggcaccccagcagatactgcgccaccgtgcgagtccagacgtcccggcaggacatgtctcaggagcaactcttcagtcaggaggtgatccaggacctgaccaacatggtgcgtgagctgctcatccagttctacaagtcgacccgcttcaagcccacacgcatcatctattatcgcggcggcgtgtccgagggacagatgaagcaggtggcttggccggagctgatcgccatcaggaaggcgtgcatcagtttggaggaggattacaggcccggcatcacctacatcgtggtccagaagcgccatcacactcgtctgttctgctccgacaaaactgaaagggtcgggaagagcgggaacgttccggccggcaccacggtggacagcaccatcacacacccgtcagagttcgacttctacctgtgcagccacgctgggattcagggaaccagtcgcccctcccactaccacgtcctgtgggatgacaactgtttcacggccgatgagctgcagctcctcacctaccagctgtgccacacctacgtccgctgcacgcgctccgtctccatcccagcaccagcctactacgccaggctcgtggccttccgtgcacgctaccacctggtggacaaggaccacgacagcgctgaaggcagccacgtgtcgggccagagtaacggccgggacccccaggcgctggcgaaggcggtccagatccactatgacacccagcacaccatgtactttgcctgaagaagcgcccgtccacctgtggattacctctgtctcttcccccctcctcctctctccttccctgtggtctcttctcttcatcctcactctgttctctgatcggcaccaaagcggctcacagaggggggggaacagtttccagccagcgttccccgtcccgccgccatttcaaccagcaatccagcactgcgagctccgcggagcgccgctgcacacacacacacacacacacacgttgagccattgttttgattggttttggtttcctctctttgaatgtctgcactcacgtgttcgtcagatacacggagcgagcgaggagcgccgctcccccggctcagcaggactctgatctactttacctcaaagcccctcggggccaaatctgtcacaaggtctggggtttatgggtggtggggagggaactctgcctgaacagagacggcggggactcgaccttttcagaacgatatattacatatatataaatatatatatatatatatacatatatatatttttcctttttatgagcgtgtttgaactttttctccttttcggtcttttacaagcgaggtgacctgggtgtccacgcccattgctactttcctcccgtcttatcgaggcgtgcgtgcgtgcatctgtacatacgtgtcctgctgtatgagtgtgtgtgcatgtgtttcatggcaggccactgaactgtaaagggaaaccacggagagaactgctgtaaatgcctcagattgttgttttatacaggacagaaagcatatatttacacggatggatccacccggaccctccgcttcagacgctttcagtcgctttgaggtggaactggacccatttgaagcggtgggcgaagccgggtcggcgtgcagcagtggcggccccggtcgggcccgtcgacccgttcgcgttggcttccggtttaaaaggcgggatctcgttcttcctcggacccgaccggatggtggcactgccgggcgcctctggactttatttcctttcggcctccttcactggtaccggccaattcattgcactgacccgggacgctaggcaagcaacggagggcactgtcgatctctcgtacctttatgcatttatacatacaacaaagcagatttgtaaaagtttaatataagatgttttgtggttatcttaaagctgttgtttagatttactagtttatcattgatgttgttgttgttgttgttgtcgatgttactattgtgatgacattttaaggttggcagcagagccacttctatagttagtgcgacttcaggaatagacgttagctgtggagctgcaggttaaagggcggcgccgggcttctttgtagcattgtggcgaggaggaggaggagttgtgtggccggcgtgagatggatggagacaatcaggaccgagtcgatggtttgttaaaatcagttggatttttagactttttaaagctgtttttcttaccgtgtgtatttgaagtatacccatccattcatttggtattttaaggcccttttttaacactggattaaacagcactgcctggtcagacactgggcgcagtgacgctggcgccccctggtgttgagattggatgctgcagaacggggaggggaaacggtcgccgtgcacgcctcatcagggcggcccgctcactttaaaaacccttttgtaataaaccacccgtaaagcagcaggtttactatgaatgagtttgagcaccttcctgttgagggcgtgcgcgccggcgggaggcgggattagttctgaaggtgttggacgtcaccagctgatcggtcgctccccgcctgtgacatcataaagcaatatgaacccagatccagacttcagacacacaaagctatacctgtcctcatcgtggtcgtctgtctgttttttttacgtttttatctttggtttcggttaatttgctcgtatcaatcagtgggaatgtgcatttggtggaatagttgtagactttagactatttcctttcaagtaactgatgtagattttgctcgcctgcatgccagagtatcgtcctttaacatgaaataaaaaaatgaactttttgaatcatatttattatgagaaagaaaaatctttttggggtaaatgtttctcagtttattagtgggactgttttagtctagcgatttctgtcccgtgtgttttaaaggctccatttgggaggtttctgaggggggggcgggggtgcggctctttgcgtcgtgttcaaatccaaacgaggtactttgtttaaacgtggacccgttaaagagcccgccccccccccctctgtgccgggccacttccggtatggccctgtggctgcgcctcccgtttccatggcaactccgacccctcacgacgcacttcctctgtgtggcggggtcccggcggcggagcaggattacctctttttaatctttttagagctccctgctcattggtcggaaaagagagcgtttaattatttttaaactttgatatcttgttatatctgaactttgtaactttgtgcttctcattttgttttcctcttcggttcctgttgggggggcgttacagctgtttttattatttctggctcggtgaggagggctcctctgattggttgtgaggggctgcggtggctccgcccccagagggccgctcactcgctgttattatgtgacttttttttttttttttttttagcctgcagggaaatcttgttaatgtgcaaagtaataaatcggtattttatggccataaa

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