LOCUS       XM_072151131           13596 bp    mRNA    linear   VRT 14-MAR-2025
DEFINITION  PREDICTED: Engystomops pustulosus argonaute RISC catalytic
            component 2 (AGO2), transcript variant X3, mRNA.
ACCESSION   XM_072151131
VERSION     XM_072151131.1
DBLINK      BioProject: PRJNA1226277
KEYWORDS    RefSeq.
SOURCE      Engystomops pustulosus (Tungara frog)
  ORGANISM  Engystomops pustulosus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Amphibia; Batrachia; Anura; Neobatrachia; Hyloidea;
            Leptodactylidae; Leiuperinae; Engystomops.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_092415) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_040894005.1-RS_2025_03
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.3
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 03/12/2025
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13596
                     /organism="Engystomops pustulosus"
                     /mol_type="mRNA"
                     /db_xref="taxon:76066"
                     /chromosome="5"
     gene            1..13596
                     /gene="AGO2"
                     /note="argonaute RISC catalytic component 2; Derived by
                     automated computational analysis using gene prediction
                     method: Gnomon. Supporting evidence includes similarity
                     to: 1 Protein"
                     /db_xref="GeneID:140132393"
     CDS             316..2904
                     /gene="AGO2"
                     /codon_start=1
                     /product="protein argonaute-2 isoform X3"
                     /protein_id="XP_072007232.1"
                     /db_xref="GeneID:140132393"
                     /translation="

MYAGAGPVIAPPPPAPPLPMPAYTFKPPPRPDFGTSGRTIKLQANFFEMDIPKIEIYHYEIDIKPEKCPRRVNREIVEHMVLHFKGQIFGDRKPVFDGRKNLYTAMPLPIARDKQVELEVTLPGEGKDRIFKVAIKWMACVSLQALHDALTGRLPSVPFETIQALDVVMRHLPSMRYTPVGRSFFTASEGCANPLGGGREVWFGFHQSVRPSLWKMMLNIDVSATAFYKAQPVIEFMCEVLDFKSIEEQQKPLTDSQRVKFTKEIKGLKVEITHCGQMKRKYRVCNVTRRPASHQTFPLQQESGQTVECTVAQYFKDRHKLVLRYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIRATARSAPDRQEEISKLMRSASFNTDPFVREFGIMVKDEMTDVTGRVLQPPSILYGGRNKAIATPVQGVWDMRNKQFHTGIEIKVWAIACFAPQRQCTELHLKTFTEQLRKISRDAGMPIQGQPCFCKYAQGADSVEPMFRHLKNTYTGLQLIVVILPGKTPVYAEVKRVGDTVLGMATQCVQMKNVQRTTPQTLSNLCLKINVKLGGVNNILLPQGRPPVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDAHPNRYCATVRVQQHRQEIIQDLAAMVRELLIQFYKSTRFKPTRIIFYRDGVSEGQFQQVLHHELLAIREACIKLEKDYQPGITFIVVQKRHHTRLFCTDRNERVGKSGNIPAGTTVDTKITHPSEFDFYLCSHAGIQGTSRPSHYHVLWDDNRFSSDELQILTYQLCHTYVRCTRSVSIPAPAYYAHLVAFRARYHLVDKEHDSAEGSHTSGQSNGRDHQALAKAVQVHQDTLRTMYFA"

     misc_feature    595..822
                     /gene="AGO2"
                     /note="N-terminal domain of argonaute; Region: ArgoN;
                     pfam16486"
                     /db_xref="CDD:465134"
     misc_feature    850..1002
                     /gene="AGO2"
                     /note="Argonaute linker 1 domain; Region: ArgoL1;
                     pfam08699"
                     /db_xref="CDD:462567"
     misc_feature    1003..1365
                     /gene="AGO2"
                     /note="PAZ domain, argonaute_like subfamily. Argonaute is
                     part of the RNA-induced silencing complex (RISC), and is
                     an endonuclease that plays a key role in the RNA
                     interference pathway. The PAZ domain has been named after
                     the proteins Piwi,Argonaut, and Zwille; Region:
                     PAZ_argonaute_like; cd02846"
                     /db_xref="CDD:239212"
     misc_feature    order(1159..1161,1204..1206,1246..1248,1258..1260,
                     1312..1314,1333..1335,1339..1341)
                     /gene="AGO2"
                     /note="nucleic acid-binding interface [nucleotide
                     binding]; other site"
                     /db_xref="CDD:239212"
     misc_feature    1498..2775
                     /gene="AGO2"
                     /note="PIWI domain, Argonaute-like subfamily. Argonaute is
                     the central component of the RNA-induced silencing complex
                     (RISC) and related complexes. The PIWI domain is the
                     C-terminal portion of Argonaute and consists of two
                     subdomains, one of which provides the...; Region:
                     Piwi_ago-like; cd04657"
                     /db_xref="CDD:240015"
     misc_feature    order(1909..1911,1921..1923,1957..1968,1975..1977,
                     1999..2001,2008..2010,2020..2022,2032..2034)
                     /gene="AGO2"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240015"
     misc_feature    order(2113..2115,2119..2121,2329..2331,2743..2745)
                     /gene="AGO2"
                     /note="active site"
                     /db_xref="CDD:240015"
     polyA_site      13596
                     /gene="AGO2"
                     /experiment="COORDINATES: polyA evidence [ECO:0006239]"
ORIGIN      

cccaccccttagttttacccgccctttttttcccctcaggtgacatcagtgagtccggctagcgactccgcccccaaaggaagacgcagcgaatggggttcccgaaacgccccgtgacgtcgccgccccgcccaccgcctttctcccctcagttcatgtcgcgtacagtctagagacggcggcggcggagtgcacggagctcgatggagacctgactgaggagagtttacccgccgggagccgccattagcgattcgtgggcaactttatctctattattttctcctatctgtgaaactgactggtggaatcaagatgtacgccggagccggccctgtgattgcccctccaccccctgcaccacctctcccaatgccagcatacactttcaaaccaccgccaaggccagattttggcacctcaggaaggactatcaagctgcaggccaacttttttgaaatggacattcccaaaatagagatttaccattatgaaatagatatcaagccagaaaaatgccctagaagagtgaacagggaaattgtagaacacatggtattgcattttaagggtcaaatcttcggagatcgaaagccagtgtttgacggtagaaaaaatctttacactgcgatgcctcttcccattgccagagacaaacaggtggaattggaagttacactgccgggggaaggaaaggatcgcatctttaaagtagcaatcaaatggatggcctgtgtgagcttacaagcgttacacgatgcattgaccggacgtcttcccagcgttccttttgagactatccaggcattagatgtggtgatgagacatttgccctctatgaggtacacgcctgtgggccgctctttctttactgcatccgaaggatgtgcaaatcctcttggtggaggtagagaagtttggtttggcttccatcaatctgttagaccatccctctggaaaatgatgctcaatatagatgtgtctgctacagcgttttacaaagcacagccagtaatagagttcatgtgtgaagttttggactttaagagcatagaggagcaacagaagcctctgactgattcccaaagggttaagtttaccaaagaaatcaaaggcttaaaagttgaaattactcactgtggacaaatgaaaaggaaatacagagtgtgcaatgtgacaagacgaccagcaagtcatcaaacattccctctccaacaggagagtgggcagacggtggagtgcaccgtcgcacagtacttcaaggatagacacaaattagtattgcgctacccccatcttccatgtttacaggtcgggcaggagcagaaacacacgtacctccctctcgaggtatgcaacatagtggcgggacaaaggtgcattaaaaaactgacagacaatcaaacctccactatgattcgtgcgacagcccgatcagctccagatcgccaagaagagatcagtaaattgatgcgcagtgcaagcttcaatactgatccctttgtccgtgagtttgggattatggtgaaagatgaaatgaccgatgtcactggcagagtccttcaacctccttcaattctttacggaggcaggaataaggccattgctacccctgtgcaaggtgtgtgggatatgaggaacaagcaatttcacaccggtattgaaataaaagtgtgggccattgcttgctttgctcctcagcggcagtgtacagaacttcacctcaagactttcactgaacagttgcggaagatatctcgtgatgccggtatgcccattcaggggcagccatgtttctgcaaatatgcccaaggtgctgacagcgtggagccaatgttcagacatcttaagaacacgtacactggtctacaactgatcgttgtaatcctccctgggaagacaccggtctatgctgaggtgaagcgcgttggagacactgtgttggggatggcaacgcagtgtgtgcagatgaagaatgtgcagcgaaccacgccacagaccctgtcaaacctctgcttaaagattaacgtcaaattaggaggagtcaataatatcctcttaccccagggaagacctccagtgtttcagcagccggtcatatttttaggagcagatgtcacacatcctcccgcaggggatggaaaaaagccttcaatcgcagcggttgtgggtagcatggatgctcatcccaatagatactgtgccactgtgagagtgcagcagcaccgacaagagattatccaggacctggccgccatggtccgagagctgctcatccagttctacaagtctacacgattcaagcccacaaggatcattttctatagggatggcgtctctgaggggcagtttcagcaggttttacatcacgagctgcttgccatcagagaagcttgcattaaattggagaaggattatcagcctggaatcaccttcatcgtagtacagaaaaggcatcatacgagactcttctgtacagataggaacgaacgggttggaaaaagtggaaatattcctgctggtaccacagtggacacaaaaatcacgcacccgtcagagtttgatttttatctatgtagtcatgctggtatacagggaacaagccgaccctcgcactatcacgttctctgggacgataaccgtttctcctcggacgaactgcaaattctaacttaccagctgtgccacacttatgttcgctgcactcgatccgtctcaatccccgctcctgcatactacgcacacttagtcgcttttagagccagatatcacctggtggataaagaacatgacagcgctgaaggcagccacacttctggtcagagtaacggccgagaccatcaagcacttgctaaggcggttcaagttcaccaggacacgctgcgtacaatgtactttgcttaactggtgctggaagcaggcactacacgaaggagcattcacgccagaccagctgcacttacctgtaacgtcgtctcagctacagggacatccagtaaaagtgcaatcaactcatagccctgtcagtctcctacggccctcggcgtcccaccagccttccgtccagaccctgaattttacttttatcttgaacttcatacctgcattgaatcatgtgaggtttgtgaaaaaccatataaatatatatatagaagcgtttacaaaaaaaaaattacccttttcaacacaaaggttaaactttactgttctctcagggctcacaaaaacaaatgaaaaacgctatgaaataattttttctctgtggaaattttgtgtcgaatatcagataaatgttacagcctagggataaaaaataacacacaaaaaagaggaagaaaatattgacatttttaaagccttaaaatattttaagtgaatttatcaagattttattacaagaaaaaaaaatcttttttttgttttttttttgttttcttcctctgacatttgtgtcaaatattctagtacataaagttatgtatcggaataaagtcggtcactaattgctatggaggtcaggcctgacctcttggtgaatataatagtattcattagggttttttttgtttttaataattgtgccattcagaaaaaaaaaaccctgaggtttagtttttaattattgcgttttctttgtggctgcactcatacttttaatatatatttttccccgaggttgctgactaatacgggtctgtggcaatgtggacagtacaatcatgttaagctaatgtacagaaaaaaataatctattttagcagtattaatgtgagatgacgtgctatttattttatttacacagatcaccagatttcttttttttaacctatggttttatgtacttgggcttaaatagtgtcctgtttaacccttgcactgacaatgcgacctgtagagccttttacaggaccaccggcagtgaatggattaaaaaaaaaaattgaaaaaaatttttgaaaaataaaaagtaaaacctcattttatgtaactgcttcatccctccagaggactcgtaaatgagactttgcaatgcaataaaaagcaatgtctctttaatccctctggttgctaacagggtttgttgtgaggagcaaaatcctaagggttaaagagaaaaaaattgtaaaactctattcttttttttttaaatgcaaaaaatacaaaaaggctgcagcgtgtaacagcagctaagatctggcttgtaaccaccaatactgacttgttcctcgctcctctgtctttggggttcccgcactgcgtccctttgtaacatccatcgttataagggaatcctcatgccgtcgcctcggagacgttcatatgcaaacctgtattagttgtctttttttattttctaataaatatctatttatctttttcccactcctcctatgctttaaggaatatgcaattctgtaatgatattcaatacaaggactcagtttttaagcaatatcatgacaagatttctacagtgctgttgtgtggctctatctggaaccaggggcacacaattttattctgtattgagccttaacactctaaacattggcatagaattgtggctgcggctctgcagctcacgctaacctacagctcccagcaagcaatgggccgtgttgggagttgtagtgcggccggagagtaatgctgtgtagtgtaagttttcattggcctaattgtaaattgaaacctaggatgttgcaggggatagggatgtatttaaatttttgtagtttttttttttttattttaaattgcccattttctccttgtgggggtaaatgattcacttgttaaatgttttaggtgctatatatttactgtctgctttgcaagcgctggtctaatcaaccaatggcaaacacggtttgtcatggccttgatagttccatagatcaaacagttagaacaatgatctccacctttaaataatgaagtcttatccgctatccttatcaactgatggagattgtgcagtgctcagagggggaaggggc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