LOCUS       XM_072136834           10864 bp    mRNA    linear   VRT 14-MAR-2025
DEFINITION  PREDICTED: Engystomops pustulosus protein argonaute-3
            (LOC140118656), transcript variant X4, mRNA.
ACCESSION   XM_072136834
VERSION     XM_072136834.1
DBLINK      BioProject: PRJNA1226277
KEYWORDS    RefSeq.
SOURCE      Engystomops pustulosus (Tungara frog)
  ORGANISM  Engystomops pustulosus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Amphibia; Batrachia; Anura; Neobatrachia; Hyloidea;
            Leptodactylidae; Leiuperinae; Engystomops.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_092412) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_040894005.1-RS_2025_03
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.3
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 03/12/2025
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10864
                     /organism="Engystomops pustulosus"
                     /mol_type="mRNA"
                     /db_xref="taxon:76066"
                     /chromosome="2"
     gene            1..10864
                     /gene="LOC140118656"
                     /note="protein argonaute-3; Derived by automated
                     computational analysis using gene prediction method:
                     Gnomon. Supporting evidence includes similarity to: 42
                     Proteins"
                     /db_xref="GeneID:140118656"
     CDS             894..3476
                     /gene="LOC140118656"
                     /codon_start=1
                     /product="protein argonaute-3 isoform X4"
                     /protein_id="XP_071992935.1"
                     /db_xref="GeneID:140118656"
                     /translation="

MEIGTAGPVGAQPFFVVPRRPGYGTMGKPIKLLANCFQVEIPKIDVYLYEVDIKPDKCPRRVNREVVDSMVQHFKVTIFGDRRPVYDGKRSLYTANPLPVASTGVDLDVTLPGEGGKDRPFKVSIKFVSRISWHLLHEVLTGRSLPEPLELDKPISTNPVHAVDVVLRHLPSMKYTPVGRSFFSAPEGYDHPLGGGREVWFGFHQSVRPAMWKMMLNIDVSATAFYKAQPVIQFMCEVLDIHNIDEQPRPLTDSHRVKFTKEIKGLKVEVTHCGTMRRKYRVCNVTRRPASHQTFPLQLENGQTVERTVAQYFREKYNLQLKYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIKATARSAPDRQEEISRLVRSANYDADPFVQEFQFKVRDEMAHVTGRVLPAPMLQYGGRNRTVATPSHGVWDMRGKQFHTGVEIKMWAIACFATQRQCREEILKGFTDQLRKISKDAGMPIQGQPCFCKYAQGADSVEPMFRHLKNTYSGLQLIIVILPGKTPVYAEVKRVGDTLLGMATQCVQVKNVIKTSPQTLSNLCLKINVKLGGINNILVPHQRPSVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDAHPSRYCATVRVQRPRQEIIHDLASMVRELLIQFYKSTRFKPTRIIFYRDGVSEGQFRQVLYYELLAIREACISLEKDYQPGITYIVVQKRHHTRLFCADRTERVGRSGNIPAGTTVDTDITHPYEFDFYLCSHAGIQGTSRPSHYHVLWDDNCFTADELQLLTYQLCHTYVRCTRSVSIPAPAYYAHLVAFRARYHLVDKEHDSAEGSHVSGQSNGRDPQALAKAVQIHQDTLRTMYFA"

     misc_feature    1110..1394
                     /gene="LOC140118656"
                     /note="N-terminal domain of argonaute; Region: ArgoN;
                     pfam16486"
                     /db_xref="CDD:465134"
     misc_feature    1422..1574
                     /gene="LOC140118656"
                     /note="Argonaute linker 1 domain; Region: ArgoL1;
                     pfam08699"
                     /db_xref="CDD:462567"
     misc_feature    1575..1937
                     /gene="LOC140118656"
                     /note="PAZ domain, argonaute_like subfamily. Argonaute is
                     part of the RNA-induced silencing complex (RISC), and is
                     an endonuclease that plays a key role in the RNA
                     interference pathway. The PAZ domain has been named after
                     the proteins Piwi,Argonaut, and Zwille; Region:
                     PAZ_argonaute_like; cd02846"
                     /db_xref="CDD:239212"
     misc_feature    order(1731..1733,1776..1778,1818..1820,1830..1832,
                     1884..1886,1905..1907,1911..1913)
                     /gene="LOC140118656"
                     /note="nucleic acid-binding interface [nucleotide
                     binding]; other site"
                     /db_xref="CDD:239212"
     misc_feature    2070..3347
                     /gene="LOC140118656"
                     /note="PIWI domain, Argonaute-like subfamily. Argonaute is
                     the central component of the RNA-induced silencing complex
                     (RISC) and related complexes. The PIWI domain is the
                     C-terminal portion of Argonaute and consists of two
                     subdomains, one of which provides the...; Region:
                     Piwi_ago-like; cd04657"
                     /db_xref="CDD:240015"
     misc_feature    order(2481..2483,2493..2495,2529..2540,2547..2549,
                     2571..2573,2580..2582,2592..2594,2604..2606)
                     /gene="LOC140118656"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240015"
     misc_feature    order(2685..2687,2691..2693,2901..2903,3315..3317)
                     /gene="LOC140118656"
                     /note="active site"
                     /db_xref="CDD:240015"
ORIGIN      

taagacccattcagtttccggaagtgacgttacggcgcgatgtgctactgtcttcctggcagtgaagcgctcggtgacgtcaggtccggcatctaggggagggggctagtggaggcagccgtcaaactcctcctcattagtgaagaaagctggaggaccaggcgctgccggcggccactgctccatcaccaagtgagccgagaataaggagggctgacggagacgtcagactatactagtggaccaacaacatccgctgacgaccaatcacaaccgggctgtcctcactccgctcaggtggtggtagggggatgccagccaatcgcaactctcctctcttcttgactcgccacttcattgccactgcttgtacggagtagaggacaggtgaagtgaactaccaatagggagagagggtacaagagcgaggagccgggggcgtgagttgaggacagccaatcggtggccagcgttttacacacagaccagctgtgacagagatgtggaccaagaggtgactcagccatctctaaggcaactgccagtaagctgctagaagtgctagtcaccacaccctctgtatccaactactgctccctcctttcacctgtcatctgaacctacaggtgtgaataagtcacagctaggtgacaactctccagggtgaagccaatcaaaagccagaattcactgtgcagccaatcagcaagtagttttcccagtgcctgtctgttggtacaggagtgccattccaagaagtgggaaacctgtcacttatcagcaaaacaagaaaccagcaacaagtgcagtgagtaaaggatcagccgcccctttacgccaacccacacactgggatcttcacatttaggacagccagacactgacctccatgaatggaaatcggaactgcaggacccgtgggggcgcagcccttttttgtggtacccagacggcctggctatggcaccatggggaagcccatcaaactgctggccaactgctttcaagtggagatcccaaagattgacgtctacctctatgaggtggacattaagcctgacaaatgtccccgcagggttaacagggaggttgttgattccatggtccagcatttcaaagtcaccatatttggtgaccgtcggccagtgtatgatggcaaaagaagcttgtacactgccaatccactgcctgtagcatctactggggtggatttggatgtaacattacctggagaaggtggaaaagatcgtcccttcaaagtttccataaaatttgtctccaggataagttggcacctcctgcatgaagtacttactgggaggtcccttccagagccattggaactggacaagccaataagcaccaaccctgtccatgctgttgatgtagtgttacggcatctgccatccatgaagtacacccctgtaggacgttccttcttctctgctccagaaggctatgaccaccctctgggagggggaagagaagtctggtttggatttcatcagtccgtcagacctgccatgtggaaaatgatgctcaatatagatgtttctgccactgccttctacaaagcacaaccagttattcagttcatgtgtgaagtgttagatattcataacattgatgaacaaccacgacctctgaccgattctcaccgggtaaaattcaccaaagagataaagggtctgaaggttgaagttactcattgtggaacaatgagacggaaataccgtgtttgtaatgtgacaaggaggcctgccagccatcagaccttccctttacagctggagaatgggcagaccgtagagagaacagtcgctcagtactttagggaaaagtataatctccagttaaagtatcctcatttgccctgtctgcaagttggacaggagcagaaacacacatacctgcctctagaagtctgtaatattgtggcaggccagcgctgcatcaagaagctgactgacaatcagacctccactatgatcaaagcaacagccaggtctgcaccagacagacaggaagagatcagccggctggtcagaagtgcaaattatgacgctgatccatttgttcaagaattccagtttaaagttcgggacgagatggctcatgtgaccgggagagtgcttccagctccaatgttgcagtatggagggcgaaatcgcacagtagccactccgagccatggggtttgggacatgcggggaaaacagtttcacactggtgtggagatcaaaatgtgggccatcgcatgctttgcaacgcagaggcagtgcagagaagaaatcttgaaaggattcactgaccagctaaggaaaatctcaaaggatgctgggatgcccatccagggtcagccatgcttctgtaaatacgcgcagggcgcggacagcgtggagccgatgttcagacacctcaaaaatacatattctgggctccaacttatcatcgttatactgccaggaaagacgccagtatatgctgaagtaaagcgagttggagacacattgctaggaatggctacacagtgtgtccaagtcaagaatgtaataaaaacatctcctcaaactctctcaaatctgtgcctaaaaatcaacgtcaaattgggagggattaataatatcttggtaccacatcaacggccatctgtgttccaacaaccagtcattttcttaggggcagatgtcactcatccacctgctggagatggcaagaaaccttcaattgctgctgttgtaggcagtatggatgcccacccaagtcgatactgtgcgactgtgcgggtacagagaccacggcaggaaattatccatgaccttgcttccatggtgagagaacttctgatccagttctacaagtccacacggtttaaaccaacacggatcatcttttatcgtgatggagtctcagaaggacagttcagacaggtcttatattatgagctgctagcaattagagaagcctgcattagtctggagaaggattaccagcctggaataacatacattgtggtacagaaaaggcatcatacccgattgttctgtgcagatcggactgaaagggttggcagaagtggaaatattccagctggtaccactgtggatacagatataacacatccatacgagtttgatttttacctctgcagccatgctggaatacagggtactagcaggccttctcactatcatgtcttgtgggatgataactgtttcactgctgatgaactccagctgctgacctaccagctgtgccacacgtatgtgcgatgcacgagatcagtctccatccctgcacctgcctattatgctcacctcgtggcattcagagctaggtaccatctcgtggacaaggagcatgacagtgctgaaggaagccacgtatctggtcagagcaatggtcgggacccacaagcccttgccaaggctgtgcaaattcaccaggataccttacgtaccatgtacttcgcgtgagaacaacttttgtccataaaaatacacaggtttgacttacaacctgaaggatctgacgtgggttttttgggcccggtagagtggttgacattctcctagcctcccatctctccatggcctattcttctggcttgctctgaagtgcaggcacagtgattgatgggagggctaatcctgggaatagaagatttttgaggaacacagcactattatgcaatatgaaaccagccaactgctaggtcgttgtaatggagcctccacttggatgcactgcttatttttttgtctttcttttactttccaatattttaacccttttatgcctttacctcaaatcgctctgaagcactagctgtctttgcaaaaaaaaaaaaaaagaaaaaaaagagaagcaaaacatagggatgtttgggagtatgattgcatatctgtagactctttgttagacagcatatctgtgtagtccttatgaaatatctttaagaaaccgtggctaatatctgatctacttactggtgacctcttaaccccctttggtgatatcgcagttagtaaaactcctgctgcacaggggcgatcatatagaaacagactcttttgtgcccaggtatgtttctatgtatcacctgtatctatgtgatgagttattgttgacctaaaagtgtatacatatacttgcgtgtgtggataatggatatgaagtagaaggatgttgtgcaaaacatctgtctcattattaggtcccacttttttgctttttatgttagacttatttgcataagacatatccttaaagtgaacaacattaaaccccccattcccacaaaaagtaccgtgttcctatgtctgcaggaggtcattttatatctttttgttattacagcatgttaaaaaaatatatatatttttccctttcttagatgcagtcagggaggcggggagctcgaccaaaagcagtcaggcaactcctgaccacccatagttaggaggcaggacaggggcgccagatgcatggagtggacggagcaaacatcggtcaggaggtggagttgccggactgcttggtggagcagctccacctccttgactgctttgattttcattcatgctgtaactgagaaatttgctaaaaagacctccaggtgacatacatattaaagaagatggtattgtttgtgaggttggggggagggacgggtttcaagtgatcccttgattctttctgcaacattatagttagcaagatgtaagtgggtattgcatgtacctagtgcggcggtcatgcaagtgaatggatatgtgattccactcttacaacaagactattgccatttatttcctgtagtgcagaaacacagtttgtttgggggtaggaaaagcagagcacatggccctctctgggtcctgatccagataccatgggccagtttttcattatctgttttatgcttttatgtcaatttattcttattcttgtgcaaaaaaagaaaacacctccataaaaaatcctgcatcccagtcgttgcccatttaaggggatgtctaccgttttaataaaattgaatggcctgaacatgtcatataaaatggttcatccccctatttgtatgctgcaaatgcttttccattcctacacattagggaaacctccttaataggatgtgggaataaaccctatccttagagtatacatggttgtgtgggaattgtaagcttttctagtactttttatatatatatataaatgtatttattagattaataacactaatttaattattttagaacctttctttgccaccattgtaatgtttttttgtgtgtgtctcatcagatatatgttttattttgctctcattttagtgcaataagcatcaatgaatttaaacccagcaatgccaaagaggcctgcacaaccctgctggcccctgtggttactttgggattaaaataaatgaaagccttaactatgaaatgaattgtgtccaaatagtgatgtctccccagaactattatggaaagtattgtgttcccttcagtgagtagtgcaatgctttgatgttcatgctttacatgtcagaatcacatatggaccaacgagatttgttttagtgcacagtgtcagaagtgttcatgttactttactatgtgaaggactagtgacatgcaagaatcgatgtcatacttagcttgggtatatgtatacctaacaagttgtttggcacaatgttgcagaatagtgtgcacagcttgccattttctcaatttggtctcaatgttatcacagtagcacctgtacatacagatgtagcctgtacatgccaaaggtaatatcagtcattcaggtgtatattcggtctgtctcttatttgcacatttcttgccaaaaaatagtagaaaacctctatggtatcaattcccagtacaggaaatatatatatgaattcaattaaatgaaattcaatgaaatgcaccctgagaaagatgtgtaatattatcattcacgttggctagtcataaagtttttgagatgccaccagaagaattttaggtggagaaaggatatttggcttgtaggaatgtacatctggctggttttgtgcaccacgtatagaagtgacattaattaagcagattaaattattaatatatatgtatatttttagattttattggaaattctagctgaacccaagcaatacttattttcgtattactctaaatttaatgtgctattttaacactaaaaaaaaaagttagttggcttttagtacacatatttgtttaaattgtttgtattcctattcatttgtatttggcataggtaatgtcatataatagttatgctttttataaccaatatagtattccaaagataaaatctttatcccctatagatgtcagtaaattattaccgattattgttacattactcacacctggtacaaacctcattacgtattcattattaagcccagtatcatgcccaatatattttcatagaaagcagtcatgtataactaccatctgaataatggttaaatagacataagactgcaaccaatatgccactgccatgacgggcatgatgatcctctggtatcatcacctgtctagactattgtaaccaagaagctaccttttttcttttttgtttagttgctgcttcttattctgacaatgttcttttacaagtatccacaagggaaatactgataatccggcatctgcagttactctgcaaacatactgaaaaaaaacacatttacctttatctcctgcagccctctgcactggcttctagaggagggctgggagagcctgatgtaaagtgcctaaagtattggctcactatgaaacatatggattgaataaataaaggggaataactttgtcaatattgcacttcttatatactgatcctcagtaacatcctgcgttatactcaattcttattaaagtatgatctacttattcctatagtcaaatcttatttagttttgctatttgtggtcaactaagcataggccacacctaaaataaataaatacatttttaaaaatgcacaaaaaacctttttctttttattataatgcattgcttagttctgcactcccctataaacagggcattccccaggtaatgttgctttacaataaccagtagatctcgcaatagtgcaagccctgtgcagactgagcctcaaggtatgtagaaaggaaatacagacagtccccgggttatgtacaagatgggttctgtaggtttgttcttaagttgaatttatatgtaagtgtaactccagacacagtatattttatgtccctgtgacgtcaggattttcaaaattttgggctgtcattggaaacaggattaataataaagcttcattgctgacacctctgataactattatgctggcaattgcagcctcggggtaaagtctgtgcattgccaatttccagaggatgcagtcaaaactgcattgcaatcagctttgaggaaggtttaggtgcagttttgtcaatttaacaggagaaagatatttgtggaacaagtttcctcttgagaatctaattcacccgttcaattcatgtctttcacctgttaaattaaaagaaactgaagctaaaactacctcaaaactgattatgatgcagttataactgcaatgtgtgactgcacccttagagcttcaccagaggtcaacgtgggtagtgaggtccctttgtaactaggggtcgtctgtgggttgagagtttgggactgtctgtagtggagctgccggcagaaatcatggagcagctctagataaggattggcaataatacagaccacaacccaaggtcagtgcaggattctttgcatgtgcaaatttttacctgttaaaggggatttctggaccatcgtgaattctctggcactcggcaccctcatgcagttgtatcagttgtttggctgccctcggaagtagatacttccattaacgttgtaattgctatgttgtggtcctgcagctcagcttccattcttttgcaccaccagatacatagaaactagtgtgaagtgttagacacaaggaatgggtcatcaaaatcaagaactctgtaaactcctttaactgaataaggggaaactctgcggtcacaaaccttgtgcttcctgtgtaatagaaatgtacatgtcatgttgtaatgctgtagaattgtgtaaaagaagtaacaatcacatcctctttgcccattttaattagggctaggtaattcattagtgacctaccattatcaccatgtgtacactcctacatagtatacttaaccaacgtagaacggagtcctagatacacaattaaaaaagacaatatatcttcattgttttaaagaagcagagctgagggagaggtagggtcaatgctgggtcaatgctgcttacctcctaactatgcttctacttcagtttttttcataggcatatctgtaaacatatttttttttacataatatcattcttcacacccccaccccaagttacaaatgagattgtggagtaaaggtctgcttgacgttctggacccctgattacgactggtgaacttttggaataaatgacaatttcatctcacattacagtggcgtgtagaggccttagcagagagcgctactggctttgcattagctgttatattatagacacaataattgaccaaactgggtattgttacatacttgtatattaaccttgtattaacattaaggttaatagtaactcttgtattgttaatatagtgtgctcagtttctgtgataatgctgtagttttactggctgccctatgtaaaagcacataccattaccattgggtcaaacaattaagaaatacattttacatctgaaaatgcagcctggcctataacagggagttactttgtagtattgagttcaaaaaatcatttgcatctgcgctccaagtggtttctataaagtaatcgagtgatcttagtgtgttgcatatctgtgataaatgaggtgacgtgttctccctagacacccctagcatacctgaaggcctgttggatatccatggctagatagatcaggctctcctgtgttggcacacagtggtggacatgtatcgctcttattgcattctttttctttttcaagcagtaacaaagtttgctgcctcaagaatttaatgcagtctaccatatttatctttgtagcccagatgtttgttcaacttgtgctacttttgggctctgaaattgtcccattcttgcgcccataagtcgcaaaacagagtatgctagacccagactttcttttgggagatactatttgggactaaaaagttgcaaaagtcagactaaaccggcaacatgtatcataaagggataaaacttaagatatatttcaatgattacatagaccaacttcaggaaacttgaaaaagtggcagctggggggcatgccatggacaatcccatatgcgtttctatgcaaacgtatgcgatcatcaatcggcacccaatgttttgcatttagagaacacaagaaacggggtgttggttgccgatcacaccatctttgggttttgattccatttcatctgacagcaccctcaggtatactttattgttcttggtaagtgagttctatatgttgcgtcacaccttgcaattactttgctttttaaaacgcaattcaacagctgaggagatgggaaattacctaattacattgatgttaacatgtgtttacaaaatgcaataacacaatagggggaatttattaagactggcgttcagaaattcctcctgccggtgctacaactcccgtaagagacttgagcctcttagtaaatatgggcaatttctgctcgagtcttggctggaattccggtggagactagagtaaatgcgacaggccaggggaaagggggacacatcccgtcctaccataccccccaccggttctacgtcccaagaagcccatagtggtgttcaggttatagaaacctgaaagttgacaggaaaatttgtatgggttctctgactggtggggaagccagactaaattggggccaatgccttaacattgtgtttataaagcgtttttgtaaacacaaatgttaacggcaatgtaattgggcaaattccctctcctcagctgttggattgtgtttcaaaatgcaatgtaaatgccacgtgtgaacttgccttaatatggtgtgatattttgctatgcatggcaagaagtgttcttggtaagtaaagggcatgatctaaaaaatagattatggagagtatatagaagaagttgtatagttatttttggaagattaaaggatagattgcacaaaatgtattattattttgacctttagaatggcacaataggtatattgttgtgaaaggaatgtgtatttctgacttgtcccttcgcgttatcctggaaccagcagtcatactatgcatttaatgttgagacaattcagattcaccatttatcaccatttgagcctccgtgtgatttccatagtagaaagcaatgcattcaagtagaaccatgcaatgcagttactagctagttgtattcatttaataaaatgcagagctgaaatgcaatggcatagaatcaaagcagaacttttattttaaaagcagaccttcctgcagtgttatcccaatgctttgtgctgtgtgacaagtttggtactactaaatctgtagttggggcaatgtggcattatgtgcttacatttctttacaacagataattgatagctatttgcaaattttccatattctggagacatttgagcaagatttccgtcaattttctttacagaacggggacaaatgtaacatattggggcaaatttatcaag

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