LOCUS       XM_072099310            7359 bp    mRNA    linear   INV 13-MAR-2025
DEFINITION  PREDICTED: Antedon mediterranea protein argonaute-2-like
            (LOC140054345), transcript variant X2, mRNA.
ACCESSION   XM_072099310
VERSION     XM_072099310.1
DBLINK      BioProject: PRJNA1232118
KEYWORDS    RefSeq.
SOURCE      Antedon mediterranea
  ORGANISM  Antedon mediterranea
            Eukaryota; Metazoa; Echinodermata; Pelmatozoa; Crinoidea;
            Articulata; Comatulida; Antedonidae; Antedon.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_092670) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_964355755.1-RS_2025_03
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.3
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 03/12/2025
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7359
                     /organism="Antedon mediterranea"
                     /mol_type="mRNA"
                     /db_xref="taxon:105859"
                     /chromosome="1"
     gene            1..7359
                     /gene="LOC140054345"
                     /note="protein argonaute-2-like; Derived by automated
                     computational analysis using gene prediction method:
                     Gnomon. Supporting evidence includes similarity to: 20
                     Proteins"
                     /db_xref="GeneID:140054345"
     CDS             111..2873
                     /gene="LOC140054345"
                     /codon_start=1
                     /product="protein argonaute-2-like isoform X2"
                     /protein_id="XP_071955411.1"
                     /db_xref="GeneID:140054345"
                     /translation="

MFPQRQRSQPGAPFVPVPQTVQSPVAQSQPVPPEQTMAPTTPPAGTTTTTTTAGAVVDSPLPSPPLEDIPCPTRPNVGTQGRPIVLRANHFQIRMPRDDIYHYDITILPEKCPRRVNREVIETLVKQCSPRIFANLRPVFDGRKNLYTKEPLPIGNDPVEMEVSLPGEGRDRSFKISLKYVAKVSLKSLEQALEGRISLIPYDAIQAIDVVMRHLPSMTYTPVGRSFFSAPESYNPPLGGGREVWFGFHQSVRPSMWKMMLNIDVSATAFYKAQSVIDFMCDVLDMRELNERRPLSDSSRVKFTKEIRGLKIEITHCGSMRRKYRVCNVTRRPAQTQTFPLQLDTGQTVDCTVGRYFMEKYHIRLQYPHLPCLQVGQELRHTYLPLEVCNIVPGQRCIKKLTDSQTSTMIKATARNAPNREKEINNLLQRAKFNEDPFIREFGINICKEMTQVQGRVLQPPKLQYGGRSLQQAYPNGGVWDMRGKQFHSGVEIRVWAIACFAPQHQCREDALRNFTSHLQKISNDAGMPIMGQPCFCKYASGADQVEPMFRHLKKNFHGLQLIIVVLPGKTPVYAEVKRVGDTLLGIATQCVQIKNVNRTSPQTLSNLCLKINVKLGGVNNILVPQQRPAVFHEPIIFLGADVTHPPAGDDKKPSIAAVVGSMDAHPSRYCAAVRIQQHRQEIIVDLANMVRDLLMQFYQSTRFKPTRIIMYRDGVSEGQFLHVLNCELRALREACMSLEVGYQPGITLIVVQKRHHTRLFCADKREQTGKSFNIPPGTTVDTGITHPTEFDFFLCSHAGIQGTSRPSHYHVLWDDNEFTADSLQTLTYQLCHTYVRCTRSVSIPAPAYYAHLVAFRARYHLVEKDHDSPSQSRNGSYVGSGGDSGEGSQHSHSGNGRSFTDMTRCVQIHEDQLKVMYFA"

     misc_feature    354..746
                     /gene="LOC140054345"
                     /note="N-terminal domain of argonaute; Region: ArgoN;
                     pfam16486"
                     /db_xref="CDD:465134"
     misc_feature    774..926
                     /gene="LOC140054345"
                     /note="Argonaute linker 1 domain; Region: ArgoL1;
                     pfam08699"
                     /db_xref="CDD:462567"
     misc_feature    927..1286
                     /gene="LOC140054345"
                     /note="PAZ domain, argonaute_like subfamily. Argonaute is
                     part of the RNA-induced silencing complex (RISC), and is
                     an endonuclease that plays a key role in the RNA
                     interference pathway. The PAZ domain has been named after
                     the proteins Piwi,Argonaut, and Zwille; Region:
                     PAZ_argonaute_like; cd02846"
                     /db_xref="CDD:239212"
     misc_feature    order(1080..1082,1125..1127,1167..1169,1179..1181,
                     1233..1235,1254..1256,1260..1262)
                     /gene="LOC140054345"
                     /note="nucleic acid-binding interface [nucleotide
                     binding]; other site"
                     /db_xref="CDD:239212"
     misc_feature    1419..2696
                     /gene="LOC140054345"
                     /note="PIWI domain, Argonaute-like subfamily. Argonaute is
                     the central component of the RNA-induced silencing complex
                     (RISC) and related complexes. The PIWI domain is the
                     C-terminal portion of Argonaute and consists of two
                     subdomains, one of which provides the...; Region:
                     Piwi_ago-like; cd04657"
                     /db_xref="CDD:240015"
     misc_feature    order(1830..1832,1842..1844,1878..1889,1896..1898,
                     1920..1922,1929..1931,1941..1943,1953..1955)
                     /gene="LOC140054345"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240015"
     misc_feature    order(2034..2036,2040..2042,2250..2252,2664..2666)
                     /gene="LOC140054345"
                     /note="active site"
                     /db_xref="CDD:240015"
     polyA_site      7359
                     /gene="LOC140054345"
                     /experiment="COORDINATES: polyA evidence [ECO:0006239]"
ORIGIN      

cgtacttggacgatttctcaatcgcgaagattgatttattgttggtaggctttgaaccaccgatacatttcatcgattaaaaccactgtaagaactatacttttttaacgatgtttcctcaacgtcaaagatctcaaccaggtgcaccgtttgttccggtcccacaaaccgtgcaatcgcctgtcgctcaatcccagccagttcctccagagcaaacaatggctcctacaacacctcctgcgggtaccaccaccaccaccaccacagcaggagcagtagttgacagtcctcttccatcgccaccattagaggacatcccgtgcccgacgcgtccaaatgttggaactcaaggtcgacccatcgtgttacgtgcaaaccactttcagatccgcatgccaagagatgacatttatcattatgatataacgatactgcctgagaaatgtcccaggagagtcaacagagaggttatagaaaccctagtcaaacagtgctctccaagaatatttgccaatctgcggccagtatttgacggaagaaagaacctatatacaaaagaaccactaccaattggtaatgatccggttgaaatggaggtgtctttaccaggagaaggacgagatcgttcctttaaaatatcacttaaatacgtagcaaaggttagcttaaagagcttagaacaggctcttgaaggcaggatctctctgataccatatgatgcaatacaagccattgatgtagttatgcgccacctaccatctatgacgtataccccagttggtcgatctttcttctcggcacctgagagctacaatccaccgcttggtggcggtagagaggtttggttcgggtttcaccagagtgtgcgcccttccatgtggaagatgatgctgaacatcgatgtttcagcaactgcgttctacaaagcccagtctgtcattgacttcatgtgtgatgtcttggacatgagggaactgaatgaaagacgaccattgtctgattcatcaagagttaaatttaccaaagagatacgaggacttaagattgaaataactcattgtggatcaatgcgaaggaaatacagagtgtgtaacgtaacaagacgaccagcacagacacagacattcccacttcaacttgatacaggacaaactgtagactgtacggtgggcagatattttatggaaaaatatcacatacgattgcaatatccacatttgccttgtttgcaagttggacaagaactaagacacacctacctaccacttgaggtgtgtaacattgtgccaggtcagcgctgcattaaaaaactgacagacagccaaacttccactatgattaaggcaactgctcgcaatgcaccaaacagagaaaaagaaattaataatttgttgcaaagggcaaagttcaatgaagatccatttattcgtgaatttggaattaatatttgtaaggaaatgacacaagtgcaaggtcgagttcttcagccacccaagctccaatatggcggaaggtctctccaacaagcttatcctaatggtggtgtatgggatatgcgaggcaaacaatttcattcaggagtggaaatacgggtttgggcaattgcctgttttgcacctcaacatcaatgcagagaggatgccctaaggaactttacatcgcacttacagaagatttcaaacgatgctggtatgcctataatgggacagccatgtttttgcaagtacgccagcggtgccgaccaagttgaaccaatgttccgtcatctcaagaaaaactttcatggtttgcagttgataatagtggtattgccaggaaaaacgcctgtctatgctgaagtgaagcgagttggcgacacattgttagggattgccactcagtgtgtacagattaaaaatgtaaacaggacatcaccgcaaacattgtccaacctctgtctgaagatcaacgttaaattaggaggcgtcaacaatattttagttcctcaacaaaggccggcagtgttccatgagccaatcatctttctcggagctgatgtcactcatccgcctgcaggtgatgacaagaagccctctattgctgctgtggtcggaagtatggatgcacatccgagtcgttactgcgcagctgttcgcatccaacaacacaggcaagagatcattgtggatttagctaatatggtgcgggatctgttgatgcagttctaccagtcaacaaggtttaaaccaaccaggatcatcatgtatcgtgatggcgtcagtgaaggacagttcctacatgtgctcaactgtgaactgcgtgcattacgggaggcttgtatgtcattagaggtcggttaccagccaggtattacgcttattgttgtacagaaacgacatcacacaaggctgttttgtgccgataaaagagaacaaactggtaaaagttttaatattcctcctggcactacagtggacactggaatcacccatcccaccgaatttgatttctttctttgtagtcatgcaggtatacaggggacaagtagaccttcccactaccatgtgttgtgggatgacaacgagtttacagctgattctcttcagacgctaacatatcaattatgccacacgtatgtgcgctgcactcgtagcgtgtcaattccagcgcccgcttattatgcacatttggtagcatttcgtgcacggtatcatcttgttgagaaagatcatgacagtcccagccaatcacgaaacggtagctatgtaggcagtggtggagacagtggggaaggaagccagcattctcacagtgggaacggccgtagttttactgacatgacacgatgcgtacaaatccacgaagatcagctgaaagtaatgtattttgcataagtcagaacgcaataaaagaaaagtgtctgtagcagaggaggttaggatattgaataaatagcagagggggatggtttattttttggaaaagctatcacacagacaatgtttgagagtggaattttgaacagaggcaggtacttattacctgtgaggtaaatatgtattacaccattttcacacagattatatcttatggtttttccattttaatgttcaacaaaattaacatactgtcttaaaactactgtacgttgcaaacatttcaccagtgaagatagcattttcttcattgaatcatttaaaaaacctttctgtgtgaaaacggtgtaatacctccgaattagtttctaatgaaaagaaagtcttgcaatacttattatctgtacatatgtatattagtttaattagctagagagagtaattactgattcaaaacattctatagatcgcaaggaggataatgttacaccccttttacactgccaagctaaaaagaacctagtgtacagaaagcaatatggcgtaaacttttgtaagccaatggagatttatatacgcggcaatgccaatcaatttttttaaataacttgaaaaacaacaaagttcaccactacataacacactctgagaataaaataggtattaactgaccaatcatgctaataaaatagcgcgcaactattctttcctcccgagatttcacaaggttcgcgtatgcgtgtgtgaaaacgtgtaataagtcgggagattgttgtaagttgtaacaatttatgcagatgtgaaaagggtattacataacaactccgcaattttcgctggcagagtaaaaggggtaatagttgcgattgcaataaagtgagtgagtatttttgaaggaagtaacaataatgttataaatgttctatagaggtaatatataagtttgggggtttttaatggtggtgtgtaattaatatattgagtgtccgaatccttttagcaatcgtgtgtgcccatcctcaatttatggcacaatttggattctagttttgcagtatgtgtaattttttttttaggggttgtaagagtatacatttttatcatctggtctaacaattacgttttataaatataaaataagtggtcagcaggaccagataaaggttaagtactgtccacaataagaaattgaccacctgatcaagtgagcagttctacattttaattgtatattgattttattaatattcaaaatttgctctgatttgagaacggttcactgaaaccaggtggtcaatgaatagtttaacaaaatgaaaaatttatttatagtgtatataactccctttactgtttattagaatgtctattactttgttaaaagtgttaatagcaaattttctatgacttttggactgttctattcagcataataggtgattacagcaatagtgcgtagaaaacatctgctcatacatatttcatataaataggcacacctgaaaggcctgtacatttttttaatactgttacaatgcaaaaaaatttacattaatttcattttaggacttcatttttaggccctgggaccccactaattaatcctggatctgccactcagggtaattggtgtagttaaagaagttattgtaaaatgtagcagagccgatgccaggtttggtatcaaaattggaggcagacaatgtggaatggggttgtgtggtcgttcatgacttccaactgtgtacaaattaacgtgatagaaagtgtatcatattaaaaatttgtcagatttcaccagactatgtaaggtttgcaaaaatgtgcagatgttcttacgtcctattgctgtcactgaatctgctttagtgcaatgaattgacctactaagcttagcagaataccataaatacatactgttttaataagtttaacatttttacaatacattacatctatgcagatgatgcacataaacataaaatattgtttaaatgtatgtaagtatagtatagtagtaaccctcctttggcacacctctattcaagcgacacccctattaaaagtcctaattatgaccctatcaagggtcctaattgtgacccctattaagggtcctaattgtgacccctattaagggtcctaattgtgacccctaataggggttcaactgttgacaagatcagttgcaacaaattagcaatcacaaataaatcttattaataattatagctttttaaaataatattgccatgcatatttattataatgctgcatattctgtaatgtaggcatccagatttatattattttttaaattatgaatttattttgtagcataaaaatcatcattttagcattgtttttatgtttaatctagacaaactgcaccacatgttttgtttaaagtgcacctaatgtttaagtttaaccttttcaggatgtttatgactaaaatgtcaagcagttgttgctcactggctaaacccagggcaccagagtttaaggggccccagatgaacagcctcaatgcaactacccagcattggaaggccccttaggtgtactaaaccagtggccttttcccctgtgttacgccacttgtacagtatctgcacatgaatacaaaccaatcatttttgcgatagctgccttcacaatgctcattgctacctcgatatcaaagttttatttgcacgatattttgcattgcataattgtcaccatcgcatcgtgccataaaatatcattacgtaaatatgactagagaagcagtagaagaatttaaataagtattttaggtactgaacagtttaataatgttcaaattgaatactattgcaactactgtgtttgtatgatagcaagggaattatagggagtactgtatccaattgtaaatttatattgaacaatttaatgatgtcatgattatgattactttgtaatcataagtgtttaagcttggttcccactgggatgtaagaacaatgcaagcgagttggccaatgagaagagacagtttgaatgatacatcgcttgggattggtcaaaacacttatgttgtgttgtgtcctagtggtagtccaagctttactgtcaataatataaaaaaacttgagttactacaaacataacttaggagaaaatattaatattgtttttctccctgctacgaagaaatctgttttaataattaatgtattgtgattgcaatactaaaaatatgtaggttaattttcatatttcatacacttatttctttgtagtttaaatgcaattctgtggcgattttaacagtcattttatcgaggtacttgtttggtactgtgcacagtaccggatccacaatttcattttagctgtaaaaatggagtaactaactcctagcgcaacttgtctgttgtagtgcgactaaagccatattattatagtggtctgtacaggccagattgtggaccacagctgtacagtaggcaatatttttaattacaaaagttatatattagtttcttcatccgtcgaccatttattaaacaataaatatttaatttaattctctcatcatgttgtgtttaaaaatactcattgtttgggtaatatacattttttgtttggtgtttatttgcggtgttttagataggccccacataattttatatctgtatatttaaaaaaaatagcaatccatgaaatatgtatatttaaacagatgtaatgcaaggctttaccttgcaaagtaaaataattttgtactgaaaaacatatgccaacattatgggaaaacaaattggaaatgtgttatatgtaaacagaaaaatattttgtacaaaattgtgaatttaaaggattattcctaattgtgaattgcctgttcatgaatcgatcacaaaatttaagatttatttagaaaattttatatagaaaataaaaatgatcatggaatcaaaatgttaatataaatgtttatttaaattttaagtggtaattttgtatgattttattttataatctgggaatccctgtacacctaaaaaatagatttaaatgtaatggttgagtaacttcatttaataacaagctgttttgtccaaaattaatgtttggtttccattctgtataaccctttcagaacactcattttttatcctaattgctaactcttaaaaagtgatttcaattccataatacacactggttgtataaatcacgtttgaaattaaacttaagtaccatgtcaatttaattattgcccttgtttggtataattttataaacacattatttgtttcatacccaaacatttataggttctaatgacttgttctacattagggattggtacattataattagtttttacaaccgttatataaattatctaataatttttgttgtaaatgaaaatattattgtattctatttatatctatcgtagcgatgtcaactaagggtttgggtttattttttaagtatatgttattaaaacattttatgatatata

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