LOCUS       XM_049612329           12009 bp    mRNA    linear   MAM 28-FEB-2023
DEFINITION  PREDICTED: Panthera uncia NRDE-2, necessary for RNA interference,
            domain containing (NRDE2), transcript variant X2, mRNA.
ACCESSION   XM_049612329
VERSION     XM_049612329.1
DBLINK      BioProject: PRJNA861302
KEYWORDS    RefSeq.
SOURCE      Panthera uncia (snow leopard)
  ORGANISM  Panthera uncia
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Laurasiatheria; Carnivora; Feliformia; Felidae;
            Pantherinae; Panthera.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_026057582) annotated using gene prediction method: Gnomon,
            supported by mRNA evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Updated annotation
            Annotation Name             :: GCF_023721935.1-RS_2023_02
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.1
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 02/27/2023
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12009
                     /organism="Panthera uncia"
                     /mol_type="mRNA"
                     /isolate="11264"
                     /db_xref="taxon:29064"
                     /chromosome="B3"
                     /map="unlocalized"
                     /sex="female"
                     /tissue_type="Tissue Culture"
                     /dev_stage="adult"
                     /collection_date="2000-05-31"
     gene            1..12009
                     /gene="NRDE2"
                     /note="NRDE-2, necessary for RNA interference, domain
                     containing; Derived by automated computational analysis
                     using gene prediction method: Gnomon. Supporting evidence
                     includes similarity to: 1 mRNA, 3 Proteins"
                     /db_xref="GeneID:125909258"
     CDS             206..3700
                     /gene="NRDE2"
                     /codon_start=1
                     /product="nuclear exosome regulator NRDE2"
                     /protein_id="XP_049468286.1"
                     /db_xref="GeneID:125909258"
                     /translation="

MALFPAFAGIGETSGSGSARKELDWLSNPSFCAGAITSLSQQTEGATALVSEGSPLTRSPLKSEPSDESDTNKKPKQTSRKKKKEKKKKRKHHHKKTRRKHGQSSSSGSEPDSDSEKDKTSRSIRDGKKESEKPNREGNAVADTGHHCIWLEDVQALTGETFRTDKKPDPANWEYKSLYRGDIARYKRKGDSCLGINPKKQCISWEGAATEKKHSHKHVERYFTKKSVGLMNIDGVAVGGKTEPPSSEPVSFIPVKGSDDVAPPVTTWLNPLGIYDQSTTQWLQGQGPSEQESKQPDSQTDREHALLKAKVEEFNRRVRENPWDIQLWMAFVAFQDEVMKSPGLYAIEEGEQEKRKRSLRLVLEKKLAILERAIESNQSSVDLKLAKLKLCSEFWEPSALAKEWQKLIFLHPNDTALWQKYLLFCQSQFSTFTVSKIHGLYGKCLSTLSAVKDGSILSHPALPGTEDAMFGLFLQQCHFLRQAGHSEKAVSLFQAMVDFTFFKPDSVKDLPTKGQVEFFEPFWDSGEPRVGEKGARGWRAWMHQQERGGWVVISPDDDDDEPEDEDQEIREKSLPRWQIWLATERSRDQRHWRPWRPDKTKKQTEEDCEDPERQVLFDDIAQSMIRLSSQDLQFQLVAAFLQFLGVPSGLSPPASCLYLAVDENSIFDDGLCDEMPLTFLNLSSSGVSCVGCSDPLGRRRWTRGHNREGEEFIRNIFHLVMPLFSGRERSQLCFSWLRYEIEKVIWCLHTKNKKRLKSQGKNCKKLAKNLLKEPENRNSFCLWKQYAHLEWLLGNTEDARKVFDTALSMAGGRDLRDRELCELGLLYAGLEAELLPDPRGAATARAVHVLTRLTEQGPCGPYSGQALAVHILKARKAYEHALQDCLGDSCVSAPAPSDSFDRLTSLVKCFMLFQYLTVGIDAAVRVYEQVLAKLKGSVPPGGSGPEDGARAQGPHGALEAVTLMHTSLLRFHTKVSLYPLAPLRQALSEALRLYPGNQVLWRAYVQIQSRSHAASETRRFFDAVTRSTKLLEPWLFAIEAEKMRKRLVESVQRVDGREVHATIPETGLTHRIRALFENALRSDNGSQCPLLWRMYLNFLVSLGNKERSKGVFYKALQNCPWAKVLYLDAVEHFPEEMQEVLDLMTEKELRVRLPVEELALLLED"

     misc_feature    686..973
                     /gene="NRDE2"
                     /note="MTR4-interacting domain (MID) found in nuclear
                     exosome regulator NRDE2 and similar proteins; Region:
                     NRDE2_MID; cd22200"
                     /db_xref="CDD:412062"
     misc_feature    order(686..712,719..724,734..739,743..745,749..793,
                     800..808,812..820,842..850,860..865,869..874,881..901,
                     926..937,947..973)
                     /gene="NRDE2"
                     /note="MTR4 binding site [polypeptide binding]; other
                     site"
                     /db_xref="CDD:412062"
     misc_feature    1142..2140
                     /gene="NRDE2"
                     /note="necessary for RNA interference; Region: NRDE-2;
                     pfam08424"
                     /db_xref="CDD:429988"
     polyA_site      12009
                     /gene="NRDE2"
                     /experiment="COORDINATES: polyA evidence [ECO:0006239]"
ORIGIN      

gtatcaattacggcgatgtggctccgccccaccccgccccgccccgcccctcctcgccctgccaggtccccgggtcaagccccagggagtgcgagtgggaaaaaccacacttcccagagtgcaccgcaaccgcgaaggcctcactaccggcgtccgctcgccggcgccatttctggagaggggaacggcggtgcggcctgcagtcatggcgctgttcccggcctttgcaggcattggtgagacttccggtagcggcagcgccaggaaagaattagactggctgagcaacccaagcttttgtgctggagccataacctctctgagccaacaaactgaaggggccacagccctggtttctgaagggtcacctctgacaaggagccctctgaaatcagagccttcggatgaaagtgacactaacaaaaagcccaaacaaacaagcagaaaaaagaagaaagagaaaaagaagaaaaggaagcatcaccacaagaaaactaggagaaaacacgggcagtccagtagcagtgggtctgagccagatagtgattctgaaaaggataaaacttccagaagcattagagacggtaaaaaggaatccgagaaaccaaaccgagaagggaacgccgtcgctgatactgggcaccactgcatctggcttgaggacgttcaggctctcacaggggaaaccttcagaacagataagaaaccggaccctgcaaactgggagtacaagtctctctaccgaggagatatagcaagatacaagaggaaaggagactcctgccttggcattaaccctaagaagcagtgcatatcctgggagggggctgccacagaaaagaagcactcacacaagcatgtcgagcgctatttcacgaagaagagcgtgggactgatgaacattgacggagtcgccgttggcggtaaaaccgaacctccctcatctgagccggtctcatttatcccagtgaagggctccgatgatgtggctcctcctgttacgacctggttgaaccctctggggatttatgatcagtccaccacgcagtggttacaaggacagggtccttcagagcaagaatcaaagcagccagactcacagacagacagagagcatgccctgctcaaggccaaggtggaggagtttaacaggagagttcgggagaatccctgggatattcagctgtggatggcatttgttgcttttcaggacgaggtcatgaagagccctggcctgtatgccatcgaggaaggagagcaggaaaagcggaagcggtctctgaggctcgttctggagaagaagctggccatcctggaacgggccatcgaaagcaaccagagcagcgtggacctgaaactcgccaagctgaagctctgctccgagttctgggagccctccgccctggccaaagagtggcagaaactgatctttctgcatcccaacgatacagccctgtggcagaaataccttttattttgccagagccagtttagcacctttacggtgtcaaaaattcacggtctgtacggcaagtgcttgagcaccttgtctgccgtgaaggatggcagcatcttatcgcaccccgccctgccggggaccgaggacgccatgtttggtctctttcttcagcaatgccacttcctgcggcaggctggtcactctgagaaggcggtctctctgttccaggccatggtcgacttcaccttcttcaaacccgacagtgtgaaagacctgcccaccaaaggacaggtggaattctttgagcccttctgggacagtggggagccccgggttggcgagaagggcgcccgaggctggagagcgtggatgcaccagcaggagcggggcggctgggtggtcatcagtccagatgatgatgatgatgagccagaagatgaggaccaggaaataagagagaagagtctgcccaggtggcagatctggcttgccaccgagcggtcccgagaccagaggcactggcggccgtggcgccctgataagaccaagaagcaaaccgaggaagactgtgaggatccagagagacaggtgctgtttgatgacattgcccagtctatgatccgtctttccagccaggatcttcagtttcagctggttgcggcctttctgcagttcctgggcgtgccttctggcctcagccctccagcctcctgcctctacctggccgtggatgagaacagcatcttcgatgacgggctttgtgacgaaatgccactgacttttctcaacctttcctcttccggtgtcagctgtgttggctgctcagacccactgggccgccggcgctggacccggggtcacaatcgagagggcgaggagttcatccgcaacatctttcaccttgtgatgcctttgttttcaggccgggagaggtctcagctctgcttctcttggttacggtacgagatcgagaaggtcatttggtgtctgcacactaaaaacaagaagaggctaaagtcacagggaaagaactgcaagaaactagccaaaaatctcctgaaggagccagaaaaccgcaacagtttctgcctctggaagcagtacgcacatctggagtggctgcttggcaacaccgaggacgccaggaaggtgttcgatacggcgctcagcatggcggggggcagagacctgagggaccgtgagctgtgtgagctcggcctcctctacgccgggctggaggcggagctgttgccggacccgagaggggccgccacggcgcgagccgttcacgtactgaccaggctgacggagcaggggccctgcgggccctactccgggcaggcgctggccgttcacatcttgaaagctcggaaggcgtacgaacacgcgctgcaggactgtttgggcgacagctgtgtgtccgctccggctcccagcgactccttcgaccgcctgactagcctggtgaaatgcttcatgctctttcagtacttgaccgtagggatcgatgcggccgtgcgggtgtacgagcaggtgctcgcaaaactgaagggctctgttcccccagggggctccggcccggaggacggtgcccgcgcccagggcccccacggcgcgctggaggccgtgacgctgatgcacacgagcctgctcaggttccacacgaaagtcagcctttaccccctggctcctctgcgccaggctctctccgaggcgttaaggttgtacccaggcaaccaggtgctttggcgggcgtacgtgcagattcagagcaggtcccacgcagccagtgagaccagaagatttttcgacgcggtcaccaggtctaccaaactcttggagccttggttgtttgcgatcgaggccgagaaaatgaggaagagactggtggagagtgttcagagggtagatggtagagaggtccacgccacaattcccgagaccggcttgacacatcgcatcagagccctgtttgaaaatgcgttgcggagcgacaacggcagccagtgccccttactgtggaggatgtacctgaattttttggtttccttaggaaacaaagaaaggagcaaaggcgtgttctacaaagcacttcagaattgtccctgggcgaaggtgctgtacctggacgctgtggagcacttccccgaagagatgcaggaggtcctggacctgatgacggagaaggagctccgggtgcgcctgcccgtcgaggagctggcgcttctgctggaggactagaccaggagggaacgggccctgccttccgggcctctcgcctgccccccccccccaacccccccaccggagccgggatgcgcaggacggggacgcatgcgtagaagcccgtgtcatggaagagaatcttgcctagaaaccagcggacactggtgggggagaagggcggcgggtcagggcacccgaggaaggtggctggccagccaagtggatgcttgcctcgatctgccccgcttccttcgcggcttcaagactgaactgttcagagaggggagaagtgccggaaatggatgaagaaagggagccacgttctgggccacagctcccgttacaccaggaaacttcgcggcaacagagcacacgtccagttcatctacctacactgaggagacagcatgcgttctggctaaatggaaatctccccatttactgcatttattgtctcgcaacacttgccaaaagtaaaacaacccacgcaacatgatgtgataaaaccacaatctcagcactaagtttctatctggttatgaccctcaggatatttcaaatggtgcagcaaaaaagaaagtgaacaaaaagacaaagcaaagaggtaaaacggtaacacttggtgaactgaggtgatgggtatgtgggcatttactattgttgcaacttttctgaagacttcaaatttccccaaatacaaagttggtgcaaaatttaaaaactctctttgaattgaagttaccgacaaagacactaacctagttctacagatgccaacgatacgcagttcagtgcaggcaccagcgtgaaaaggccccacacacgccgtccctacctgactgtggcacttacccctacctgacagaaacccaggtcctcacatacaaagcaggctggacccacactcagtgctgccgagcctcgccctaattctggtcgtctgggaagaaacgtcctcacacacacacaactaagaggtacagtccagcccaggttctcaccttgatgtcggccccagagaggtcatctttagccatgatcaagtcgtccagggtcacatcatcggccagcgtcatcctgctcgtgtggatctgaaagatgcgcctcttggtcttttcatcgggcagggggaactcaattttcctgtcaatgcggcctgcgggtataaaaggtttccagtcacacccctgtggctccccccaggctctgctgtggctcggggagcctgtctcctcacacctgcagtttcagagggaaacttcccttgtgggaagggtccaaatcttgaaaagtgaacactgctctttgtaaatatccacgtgtgaaatcatcccgtttaaaagaaatcctctctcctctggtaccttcagaaacacacaaactagccactgctgcttttggtgctacccgaatgcgaaattaactggagctaccataggagctatttcttcagaaaaaagaatattcattacgggaaagagaaacggtcacaacttaagactgcgatggaaagggccacactacacctactacatcatgttcttagccattacctcagtggaacgcagcttttgggaagttattctacacttcagtaatgtatttgccttgatgtgtatcatttttaaatcccctgaaagtgcacttaaacacgaacacatagaaacacactcatcctccgtgatgggacaaaagcaaatctgacctggtctgataagtgctggatccaaagtttctattcggtttgtggccatgataactttcacgtcaccccttgaatcaaatccatccaactggttcaacagttccaacattgttcgctggatttctctttcaccaccagaatttgaatcgtatctttgaaaaagaagagaagggatgaaaaccgaactgatctcctgctgaaggggaattataaaaacacctctgatgccctcaattctagcaacaaaatcagcacagcctcccaaccaccacataatcttacttaaactgtagatgttttcgtgcctgagatggcatcattaattcagattgcacgttcggtatctgagcccctgacccaactatgagctcctggaaagccttgtgacaatggctgtgtccccagagtgcagatgatgcctggctcagagtggaaactcaaggcatggctgcacactaatctccagacggtcacgactcccaggtggttgtcacctggcaccccggagcagagcctaaagcatccacagccacatacagtaggagccaaggcaaacccatgtgctggccacacaggcagtgctccgtgatcattctcagacaagagctacaaggtctttacctctaaatgacacaaagagaaaaatgtacctttttgttccaatggcatcaatttcatcaataaacacgatggatggtgcgtgttcttctgcaactcgaaataactcccgaacgagtttgggcccatcacctaggtacttctgaataagttcagagccaaccactcttaggaaagtggctgaggtttggtttgctactgctttggctaataaggttttacctattttaggttagaaaaagaaagacaaagtgaaaaaaccctcaagtgctgtcttaacttgaaaaccctgtcactaccacctaaaacgggcataaaaaacgggcagatccctccgatgaaaaggcatgccaccattccagtgtcttgacattatgttctccaaggcagataagaggaaattcagaacgttctgattatttttaaccaccttcctaaagtcatcatcatagtgtcagaaaatacactttactgggttattcgaacttgcatagaaattgcaagttattaacccaacgcatgccaccagggtttgagatccaaactctactctgtgaaacaatcagtgatggaaaaacggcctgagaaccctaggtctgacctaaggaaacactctttagcaccaggcctgcagcccccggggccacccgaggagtcgtggctgtgctggctgggcttccccgaggctcagctgagacggggccagctcccactgaaacccacaaatgatagctcttcagacttttctcagcctctgttgtgtccttgtgaggaacttcctctcaccaatctcagggaagatctcaaacatttccagtcctgccccgttccttgagatttctatttcaggtcaaaaccaagagatctggcagccattttgtgaactttagggctggaagaccttggcccctttgacggtgagtgaggacaggggcacagggccaagcaggagcatcaggacacggtggcagttagagcagggctggtaaaggaccaaccttaggcccgcaggggaaggggaggaggctgccggggagtaacacagaagacttagttcaagtgggaagaaggacgaggcgctaagctttactacaagatgtggccacgacatccttgtggtgagacagacagagggctgggtgtgaaggcttaaagtgcggagattccccaagagaaccgactaaggctgctgaacagaagacaaaaaggacctaagtgggtggcatctttcagtccagaagcggccaaggccagctgctgtggcactgcagtagccagagggctcaccgaaccggcttcttcctcctcctggcaggcccaccaccctgtacttcaccccagctcctcagatcggtccccagcagtgcacatgcaccaaaggaggcctctcttccagcctcagccctgtcgccagctcactcccagagcgaccgcggccttgggctagtagtcactggcctctctgacacttgatttcctgggctgtgagaggacaggctgcagaagctccatgcctccggagctcgtctccacttgtctcccagtccctccactgtgggcagtccccagtcctctcaggatcctcctgggaacgtggtttcaattcactcaaataactgtgcttcctctggaatttacacagcgatgtactgctaagtcctggactgtacaacttactcaggtcaactagtcgcacggtgaaccaaatatatgtatttttatcttcatctatgtgttcaatattatattcattcagtaccaatggaccagagaatgaacctaatctgaacagcacagaataggttttaaatcagcggttctccaccatggttctgatgtctggctacacactccagatgcagatcgggattttcagggatgggaccaaatattggtattttttaaagctccagggtgatcctagtgtacagccaaggttgagcatcctggtttatttcctgtcttataaagtatatttaatagactcacactaatcttgaattatgaatacttgaaagccttatgttttacagcatataaatgaggtatacaagaggagttctaagaagctgtaaatgcattcacactgagcaggcatttggggtgggggctgcctactggtgttcgttttttgttgcaattcctactactgtttttaacacgttaagtctgttaagtctacaatttcaagtctaagataattcagtttctaagcatctactatacagataaatatacttttgtgtaataggtatgtttccgtatttattccaaaaacgtccaaaatgccgcttagaaaactttgttttaaactaaggaattgtggcatccttactcaagaattaattataacaagctaatactgcagctcaggagatcagctgagtgcccagagagtgtcaaacacagagcatacgtacctgtgccaggtggtccatagagaatgaccccttttgggggctttatacccatctcttcataatattcaggatgagtgagaggaagctccacggattcctagagaaaacaaacactggtaaaaataaaacgctttcctactaaaattatcagatccattaaccggcgtcaagaatatagctcactttcatcaaaaataagctaaaacctcacttactactgttagagacactgtgggtgacttcatctagcagcaaggacacattgaagaggacatctgtcccgcctgagctgccgccccctgcctattgctcccaatttccctctaactttccagaagcaacatttaagccttgagcaacatttaataagctcttcctgagcaaaagaacttcaagtgaaaatacaccctttccatttaaactacagacaggagctgggatgattttctgattgctgttacaccgacagacacgctggctagagatcgagctgtctgagaggtgccgggtggagaccggggacagctgtgcatgacctccctatagacgagacgccgtgcaggtggctggcccacagccaacctgaatgaccactgcacttcccccgactcacactgtccccacatcactggaggtttccagatggccacagctgaagttaacacagatggaaacattatatattcacacacacaataaacactgcatataaaaaggattaagctgcagctgctgaagcaacaatcacccaatttatttccattacctaactcggtattaacaactacaaaagagcacactggcaagctctgttactgcttgatcagtacagaaaaaacaaaatatgcaaaataactctaagcaccctaaatgaggcagcggtaagaaatctgaagtctaaaaacccagagccggtgcctagacctaccttaatttcctggatttggttgtccaaccccccaatatcagcataggtttcctggggggccttttctactttcatcaccgtgaccaagggatccgtgtcatccatgagcacccctatgacggcatgtacctacagagaacgttaagttttactgcagggacgtgaggtttgcacaatccatttacaacagtgcaatgccaatcaccagaacggcacgaatgggcggatccgagaggaaggaggaagcatccctaattaggggcttcgagaccggcaacttgccttgtggttgagcaggaccgaacagcctggctccagcaggtccttgtctacaaaggagagaatgctgacgtagtgttctgagcccacagacgtggacacgatggcgtgattgtcatcaatgatctcttccaacgttcctaccgacatcggggtccccctcagatcatccacctttgatctctcctcctatgtaagagtatgccatgaaggaaaagcgggttagacctaaaaagtagacaatgctgacagttttactagagttactagattttactacgtttacaaagcaggtggtgctttaaaagcagctaatttatgattcgtaagagcctgcacaggtcaactgcttgaatttcacttattcactgaattctatttaataactaaaaccgtcaatttgtaaaatcaaagttacagtaatttctccgtttacctcttgcttttcttctaaaggcttcatttgttcctgatttctaatgaattcttcctccatgagaagatagtctttaattctctctaacttcagtaattttaagcggcactgagtgtgaggtgtcactgtaattaaaaaagttttactgtatgtggagcttgacgttcgcaatgaattaagtccacccataagaaaaaaaggttaagacttaaataagctaataagcaggtgcctaaatgcaaagggaagagagactttggcagttctaagcaagcaggtaaaaacgggacgagttccatgaccagacagctaatacaaaatgacatacactaagtgaactgtctttatatataagaaaaagagaactcaaggacccaaaagatagtttgcttcggtgttttattccccataggtgctaataaaaatagctacaagtgctgttcagtcagaatacatgctgcatgctagctagaacaaaagcatccacttgtaggaggaccgtgagatatcagtggtattaacataggcggcaaacattttttccacactcttctgcgttcttctggcttgaagtcccctgctgcacaggaatcttagcatatatagacaaaaatgccaatcttttcttttgtggttcctaggtttcttcaaaatgtctctccttccctgaggttaaatatattcctgaatttttgttcatttacttattttcacgtctgtactacatctggcaaaaagggatctaatttcattttccctcaacagcaaataaaatatgccagccatttatgttaaatacttcatcctcttcccacagaactcagacatcaccttaccatacagtaatgacccatgtgacctgacttcctatgctcttctggcaatcggaggacctagactttaccgataccatattgactcattattcattttttcaaattgtcagattcctttccttcttaaccaaccaacatttattgaaattatttcatctaagcaggaagagacttgtttagatggggatgggagggttatgtaagacgaagtgatgagaagaaggcagccatgggaaggagaggggggaagaacgtttttaagcaaacggcaaaagccctaatgtggataagagtcaaagaacacaaggcagggccgtgtgaacgacagcaaggggagtgacgggctgaggacagaggtgggctgggtcaggtcacctgatctgaatctcagtctatgttcagtatgaagccactgaaacagctggtgttcaggaatcccaagatggacattccaacacagtggcgttccatttcttcggggaataagcgtgttttctaagttattcaagctcaatctgacttttctaatctcaaactacactcttcatacctcagaggagctggctgtttaagtgagacatggttgtatgccgtctgcacgccgctaacaccaaaacacagcttttaccgcagcccagaggagggccacacccgcgagcagagctgcagccaccccgtttcctgactccacattccgacctctacgctacacacacagacggctctcatggaaccgacacagcagggaggaaccattacccagtggcagcttgctggcggcatctggtccctttgttttcttcttctttttccctactctggttggtacaggaggttcgtacttctttttcttgtccttgaatttttagaacagcaggaaaaaacaaaaaccacacagatcatatcaaaacacaaacttctgtaaggccatcatctatttctcggggctgtaattttccttatctttcagaatgctattaaaaactccttttacaaataaagctaggtttacacttctgcaattggtgaatgtaactgatctttttatttttttatttttaaaagtttatttattttgagtgaatccgtatcagaggcagag

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