LOCUS       XM_049568454            3357 bp    mRNA    linear   VRT 23-JUL-2022
DEFINITION  PREDICTED: Epinephelus fuscoguttatus procollagen C-endopeptidase
            enhancer b (pcolceb), transcript variant X3, mRNA.
ACCESSION   XM_049568454
VERSION     XM_049568454.1
DBLINK      BioProject: PRJNA859906
KEYWORDS    RefSeq.
SOURCE      Epinephelus fuscoguttatus (brown-marbled grouper)
  ORGANISM  Epinephelus fuscoguttatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Perciformes; Serranoidei; Serranidae;
            Epinephelinae; Epinephelini; Epinephelus.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_064774) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: Epinephelus fuscoguttatus Annotation
                                           Release 100
            Annotation Version          :: 100
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.0
            Annotation Method           :: Best-placed RefSeq; Gnomon
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3357
                     /organism="Epinephelus fuscoguttatus"
                     /mol_type="mRNA"
                     /db_xref="taxon:293821"
                     /country="China"
                     /collection_date="2018-12"
                     /linkage_group="LG23"
                     /note="scaffold1"
     gene            1..3357
                     /gene="pcolceb"
                     /note="procollagen C-endopeptidase enhancer b; Derived by
                     automated computational analysis using gene prediction
                     method: Gnomon. Supporting evidence includes similarity
                     to: 1 Protein"
                     /db_xref="GeneID:125883850"
     CDS             118..1641
                     /gene="pcolceb"
                     /codon_start=1
                     /product="procollagen C-endopeptidase enhancer b isoform
                     X3"
                     /protein_id="XP_049424411.1"
                     /db_xref="GeneID:125883850"
                     /translation="

MLSFRLFDMEADPTCRYDYLDVYNGHSRLVQKLGRFCGTFRPGALIATSNTMMLEMVSDEATGGRGFLAAFSAGKPHVEENQFCGGRLTKSQGSVKTPNWPNSNYPAGISCSWHISVETGNVIEVKFEKLDLEPDTYCRYDYVALFNGGERDNSRRIGKFCGDRPPGTIVTNGNELLVQFVSDLSITSDGFMAHYASVPRGSRRPTAGGDFIYGPQTTTAPQKPAVKPIKPTKPPPKPKPAPKPKPTPKPAKKPLVKKPPPRKPAAKPTPKPKPAKPTPKPKPAKPTPKAPVKKPTPKPGLRPTPKPKIIKPTLKPSIKTKPTRKPALKTKPTLKPAVKPVKPTPKSRAKPTPKPKIKPKVTPKPGVSKTVTKKPLVTKKPLPLNPVCTQPCKRTGTLQSSFCPHDFVITGKITSLTPGERGSVSVEVFLIKAYKPGRLNIARSGPIMSATLISTCKRCPGLIKGRNYVLMGKVDALGNGHLSPSSFTLLYKPIHAKALASLAGKPC"

     misc_feature    <118..336
                     /gene="pcolceb"
                     /note="CUB domain; extracellular domain; present in
                     proteins mostly known to be involved in development; not
                     found in prokaryotes, plants and yeast; Region: CUB;
                     cd00041"
                     /db_xref="CDD:238001"
     misc_feature    367..708
                     /gene="pcolceb"
                     /note="CUB domain; extracellular domain; present in
                     proteins mostly known to be involved in development; not
                     found in prokaryotes, plants and yeast; Region: CUB;
                     cd00041"
                     /db_xref="CDD:238001"
     misc_feature    order(391..393,397..399,403..405,487..489,502..504,
                     619..621,691..693,697..699,703..708)
                     /gene="pcolceb"
                     /note="heterodimerization interface [polypeptide binding];
                     other site"
                     /db_xref="CDD:238001"
     misc_feature    <1000..>1158
                     /gene="pcolceb"
                     /note="transport protein TonB; Provisional; Region:
                     PRK10819"
                     /db_xref="CDD:236768"
     misc_feature    1264..1638
                     /gene="pcolceb"
                     /note="NTR domain, PCOLCE subfamily; Procollagen
                     C-endopeptidase enhancers (PCOLCEs) are extracellular
                     matrix proteins that enhance the activity of procollagen
                     C-proteases, by binding to the procollagen I C-peptide.
                     They contain a C-terminal NTR domain, which...; Region:
                     NTR_PCOLCE; cd03576"
                     /db_xref="CDD:239631"
     polyA_site      3357
                     /gene="pcolceb"
                     /experiment="COORDINATES: polyA evidence [ECO:0006239]"
ORIGIN      

caggactgtgtgtgggtgcacaggcagcacctgtgtcacctttatcagtggatagagttagttttctaactaattacaagtctgggtaccttcaaggtcccagagggtcacgtggtcatgctatctttccgcctcttcgacatggaggccgaccccacctgtcgctacgactacctggacgtctacaacggtcactcccgcctggtgcagaagctgggtcgtttctgtgggacgttccggcccggcgccctcatcgccacctccaacaccatgatgttagagatggtgtcagacgaggccacagggggaagaggctttctcgccgccttcagcgctgggaagccacatgtggaagagaaccagttctgcggaggacgtctgaccaagtctcagggctctgtcaagacgcccaactggcccaactctaattacccagcaggcatcagctgctcctggcacatctccgtagaaacaggcaacgtgattgaggtgaagtttgagaagctggacctggagcctgacacgtactgtcgctacgactatgtggcgctgtttaacgggggagagagggacaactcgcggcggatcgggaagttctgcggagacaggccgccaggaacaatagtgacaaacgggaacgagctcctcgtccagttcgtctccgacctcagcattacctcagatggcttcatggcccactacgcaagtgtgcctcgcgggtctcggaggcccaccgccgggggagacttcatctacggacctcagacgaccacagcgccacaaaaaccagccgtgaagccgatcaaacccaccaaaccacctcccaaacctaagcctgcccccaagcctaaacccaccccaaaaccagccaagaaaccactggtgaagaaaccgccgccacgcaaacctgccgccaagcccacacctaaacccaagccagctaaacccacacctaaacccaagccagctaaacccacacctaaagcacctgtgaaaaaacccacacctaaacctggacttagacctacacccaaacctaagatcattaagccgacgctcaaaccgagcatcaaaactaaacccacacgtaagcctgcactgaagaccaagcccaccttaaaacctgccgtcaaaccagtaaagccaaccccaaagagcagagccaaacctacacccaaacccaagattaaacccaaagtgacgccgaaacctggagttagcaagacggtgacgaagaagcctctggtgaccaagaaacctttaccactgaacccagtgtgcacacaaccctgtaagaggacaggaaccctccagtccagcttctgccctcatgacttcgtgataacaggtaagatcacatctttgacccctggtgagaggggctcagtgtcagtcgaggtgttcctgatcaaggcctataagccaggacgcctgaacatcgccagatcagggcccatcatgtcggccacactgatctccacctgcaagagatgccctgggctaattaaaggacgcaactacgtgttgatgggaaaagtcgacgcactgggcaatggccatctcagcccctccagcttcaccctcctctacaagcccatccacgccaaagcactggccagcctggcgggcaaaccgtgctgacatcacagcccagcccaccaatcagatggacagtcctctgactctctctctgatcacatgtaactttacataagagtgaaaatattatgagtaatatccaaacctgatttttatacaatgctgccgtgaaaaatgatttttaaaaaacatacaacattataaagtgttatattttgtattttgtatgtaagttatgatatgtctgtgattaatggtgcacatcaataaaccatctggtgcttaacatgatctaatatctcaattctactgttcacagtgaaatatgacgtgaaacactttaaggtaaaatttttattaggcacaaacatcaaaacatagataaatattttaatgcccaaagaaggcattatgtttcgggttgtcctgccatcccattctcatgaacatggtatctcaagaacaccttaagagaatttctccaactttggcacaaacatcaacttggattaaaggatgagctgattagactgtggtggtcatagtcaaaggttgctgcgatgattcttgtgatcgtaatatctcaagaacacttcagggtcatttttttcagatttggcacaaacgtctacttgaactcaatgatgaactgattcgattttggtggtcaaaggtcaaggtcactataaccttgtcatttgcattcttgttaacgtgatatctcaagaaaaccttacgggaattccttcaactttggcacaaatgtctgcttaggctcaagaataaactgattagattttggtggtcataggtcactgtgacctcgtccccagcattctcgttagcgggatatcttaagaacatggtaagggtatttcttcaactttggcacaaacatccacttggactcaaggatgaactgattatattttggtggttgtagtttaaaggtcaaggtcaccgtgacctccttttgtctcattctcatgaaagtgatatgtcaagaacacttcagggaaacattttcaattttacacaactgtcaatggtcaaggtcactgtgacctccttctgtcacattgttgttaatgtgatagctaaagaacatagtaagggatttttttcaacacaaacatgaatgaactcataagattttggtcattgtaggtcaaaggtcaaggtcactgtgaccttctgtcttatttttgtgaatggtatatttcaagaactctctgggttgtttttcaaatttggcacatttgttcacttggactcaagaattaactgattagaatgtagtggtcaaaggtcaaggtcactgtgatcttctgtctcattcttgtgatcgtgatatctcaagaactctctgggttctttttcaagtttggtaccgatgtgactaacaccttaaggaaatttcttcaactttggcacaaacatccacttggacttagggataagattagggggtcaaaggtcactgtgaccgttcacccatctcattcttgtgaatgtcatatctcaaaaaggctgtggaagaattttcttaaatttggcacaaatgtctacttggactcaagaatgaaccaattagattttaggggtcaaaggtcaaggtcactgtgacctcattaatcatgtttttgcccataagtcaagaattcttgtgctaattatgacaaaacatttgtaaaaactattgtattctgtatgtaaattataatattttacagaacaataaaccatatggtgcattaa

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