LOCUS       XM_037021282            6416 bp    mRNA    linear   MAM 17-APR-2025
DEFINITION  PREDICTED: Manis javanica argonaute RISC component 4 (AGO4),
            transcript variant X3, mRNA.
ACCESSION   XM_037021282
VERSION     XM_037021282.2
DBLINK      BioProject: PRJNA1247575
KEYWORDS    RefSeq.
SOURCE      Manis javanica (Malayan pangolin)
  ORGANISM  Manis javanica
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Laurasiatheria; Pholidota; Manidae; Manis.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_133159) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Apr 17, 2025 this sequence version replaced XM_037021282.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_040802235.1-RS_2025_04
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.3
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 04/14/2025
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6416
                     /organism="Manis javanica"
                     /mol_type="mRNA"
                     /isolate="MJ-LG"
                     /db_xref="taxon:9974"
                     /chromosome="4"
                     /sex="male"
                     /tissue_type="blood"
                     /dev_stage="adult"
     gene            1..6416
                     /gene="AGO4"
                     /note="argonaute RISC component 4; Derived by automated
                     computational analysis using gene prediction method:
                     Gnomon. Supporting evidence includes similarity to: 1
                     Protein"
                     /db_xref="GeneID:108397529"
     CDS             266..2722
                     /gene="AGO4"
                     /codon_start=1
                     /product="protein argonaute-4 isoform X3"
                     /protein_id="XP_036877177.1"
                     /db_xref="GeneID:108397529"
                     /translation="

MEALGPGPPASLFQPPRRPGLGTVGKPIRLLANHFQVQIPKIDVYHYDVDIKPEKRPRRVNREVVDTMVRHFKMQIFGDRQPGYDGKRNMYTAHPLPIGRDRVDMEVTLPGEGKDQTFKVSVQWVSVVSLQLLLEALAGHLNEVPDDSVQALDVITRHLPSMRYTPVGRSFFSPPEGYYHPLGGGREVWFGFHQSVRPAVWNMMLNIDVSATAFYRAQPIIEFMCEVLDIQNINEQTKPLTDSQRVKFTKEIRGLKVEVTHCGQMKRKYRVCNVTRRPASHQTFPLQLENGQAMECTVAQYFKQKYSLQLKYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIKATARSAPDRQEEISRLNKTVATPNQGVWDMRGKQFYAGIEIKVWAVACFAPQKQCREDLLKSFTDQLRKISKDAGMPIQGQPCFCKYAQGADSVEPMFKHLKMTYVGLQLIVVILPGKTPVYAEVKRVGDTLLGMATQCVQVKNVVKTSPQTLSNLCLKINAKLGGINNVLVPHQRPSVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDGHPSRYCATVRVQTSRQEISQELLYSQEVIQDLTNMVRELLIQFYKSTRFKPTRIIYYRGGVSEGQMKQVAWPELIAIRKACISLEEDYRPGITYIVVQKRHHTRLFCADKTERVGKSGNVPAGTTVDSTITHPSEFDFYLCSHAGIQGTSRPSHYQVLWDDNCFTADELQLLTYQLCHTYVRCTRSVSIPAPAYYARLVAFRARYHLVDKDHDSAEGSHVSGQSNGRDPQALAKAVQIHHDTQHTMYFA"

     misc_feature    341..730
                     /gene="AGO4"
                     /note="N-terminal domain of argonaute; Region: ArgoN;
                     pfam16486"
                     /db_xref="CDD:465134"
     misc_feature    761..913
                     /gene="AGO4"
                     /note="Argonaute linker 1 domain; Region: ArgoL1;
                     pfam08699"
                     /db_xref="CDD:462567"
     misc_feature    914..1276
                     /gene="AGO4"
                     /note="PAZ domain, argonaute_like subfamily. Argonaute is
                     part of the RNA-induced silencing complex (RISC), and is
                     an endonuclease that plays a key role in the RNA
                     interference pathway. The PAZ domain has been named after
                     the proteins Piwi,Argonaut, and Zwille; Region:
                     PAZ_argonaute_like; cd02846"
                     /db_xref="CDD:239212"
     misc_feature    order(1070..1072,1115..1117,1157..1159,1169..1171,
                     1223..1225,1244..1246,1250..1252)
                     /gene="AGO4"
                     /note="nucleic acid-binding interface [nucleotide
                     binding]; other site"
                     /db_xref="CDD:239212"
     misc_feature    1319..2593
                     /gene="AGO4"
                     /note="PIWI domain, Argonaute-like subfamily. Argonaute is
                     the central component of the RNA-induced silencing complex
                     (RISC) and related complexes. The PIWI domain is the
                     C-terminal portion of Argonaute and consists of two
                     subdomains, one of which provides the...; Region:
                     Piwi_ago-like; cd04657"
                     /db_xref="CDD:240015"
     misc_feature    order(1697..1699,1709..1711,1745..1756,1763..1765,
                     1787..1789,1796..1798,1808..1810,1820..1822)
                     /gene="AGO4"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240015"
     misc_feature    order(1901..1903,1907..1909,2147..2149,2561..2563)
                     /gene="AGO4"
                     /note="active site"
                     /db_xref="CDD:240015"
     polyA_site      6416
                     /gene="AGO4"
                     /experiment="COORDINATES: polyA evidence [ECO:0006239]"
ORIGIN      

gcacgcgcactggctcccgttcccgctggcagcggcggcggcggggattgttttgttgtcgctgaggccggaagagccgcgggagccgggtccctgtccctggccgggcgccgccgccgccccctgcccagcgcccgcgtctccgcggcgcccacccagcgccaatattccggagatcaagcgttacgcggcggcggcggcggcggcggcggggcccggagcgggaggcgccggggaccggggcgaggcggtccccgccgccgccatggaggcgctgggacccggacctccagccagcctgtttcagccacctcgtcgtcctggccttggaactgttggaaaaccaattcgactgttagccaatcattttcaggttcagattcctaaaatagatgtgtatcactatgatgtggatattaaaccagaaaaacggccccgcagagtcaacagagaggtagtagatacaatggtgcggcacttcaagatgcaaatatttggtgatcggcagcctggctatgacggcaaaagaaacatgtatacagcacacccactgccaattggacgggatagggttgatatggaagtgacccttccaggtgagggtaaagaccaaacctttaaagtgtctgttcagtgggtatcagttgtgagtcttcagttgcttttagaagctttggcagggcacttgaatgaagttccagatgattcagtacaagcacttgatgttatcacacgacaccttccctcgatgaggtatacacctgtgggccgttcctttttctcacctcctgaaggttactaccaccctctgggagggggcagagaggtctggtttggctttcatcagtccgtgagacctgctgtgtggaatatgatgctcaacattgatgtgtctgcaactgctttctaccgggctcagcctatcattgagttcatgtgtgaagtgttagacatacagaacatcaatgaacagactaaacctctaacagactcccagcgtgtgaaatttaccaaagaaatcagaggtcttaaagttgaggtcacgcactgtggacagatgaaacgaaaatatcgagtttgtaatgtgactagacggccggccagtcatcaaacttttcctttacagctagaaaatggtcaagccatggaatgtacagtagctcaatattttaagcaaaagtatagtctacagctgaaatacccccatcttccctgtctccaggtgggacaagaacaaaaacatacatacctgccacttgaggtctgtaacatagtggcaggacagcggtgcataaagaagctcacagacaatcagacttccacgatgatcaaagccactgcaagatccgctcctgacagacaggaagaaatcagtagactgaataaaacagtagccacacccaaccagggtgtctgggacatgcgaggaaagcagttttatgctggcattgaaattaaagtttgggcagttgcttgttttgcgcctcagaaacaatgtagggaagatttactaaagagtttcactgaccagctccgtaaaatctctaaggatgcaggaatgcccatccaaggtcaaccatgtttctgcaagtatgcccaaggtgcagacagcgtggagcccatgtttaaacatctgaaaatgacatacgtgggcctacagctaatagtggttatcttgcctgggaagacaccagtatatgcggaggtgaaacgtgttggagataccctcctgggtatggccacacagtgtgtccaggtaaaaaatgttgtgaagacctcaccccaaaccctttccaacctttgcctgaagataaatgcaaagcttggaggaattaacaatgtgcttgtacctcatcaaaggccctcggtgttccagcagcccgtcatcttcttgggagcagatgtcacgcaccctcctgcaggggatgggaagaagccttccattgccgctgttgtgggcagtatggatggtcaccctagccggtactgtgccactgttcgggtgcagacctcccgccaggagatctcccaggagctcctctatagtcaggaggtaatccaggaccttaccaacatggttcgagagctgctgatccagttctacaaatccacacgcttcaaacccactcggatcatctattaccgtggaggggtatccgagggacaaatgaaacaggtagcttggccagagctaatagcaattcgaaaggcatgtattagtttggaagaagattaccggccaggaataacttacattgtggtgcagaaaagacatcatacacgactcttctgtgcagataaaacagagagggtggggaaaagtggcaatgtgcctgcaggcactacagtggatagcaccatcacacatccatctgagtttgatttctacctctgtagtcatgcaggaattcagggaaccagccgtccttcacattaccaggtcttgtgggatgacaactgcttcactgcagatgagctccagctactcacttaccagctatgtcacacctatgtgcggtgcacacgctcggtctctattccggcccctgcatattatgcccggcttgtagcattcagggcaaggtatcatctggtggataaagatcatgacagtgcggaaggcagtcatgtgtcaggacagagcaatggtcgggatcctcaggccttggctaaggctgtgcagatccaccatgatacccagcacacaatgtattttgcctgagagtctcagaaaaagaactcaaccaatttggcaccctcatgcagcctccaatgtttcaaatgcttactgcctctacagagccacgttgacttcagatggtcttctaccagcagctcggaatagttgcactgaatctatacttcgcagcactgtctgatgcatcaacaaaattgagccattttttaaaagtaatagatactcatagattgtcttttctgatgcactggactgaatttttacctcatcatgcaaaggctgatcagcctgaactttctaaaggactttaccagaaaggtttctatggaatattttgctagctgtggtgttcaccgcatccctctagtcttacaagagaagattattccttttttctgtattcatcaaatggggtgtatgcataaataagtgggagagaaaaaccaaacaatctacttcagttcagcgtaattatttaattgtgtgggtccatggactttttaatggccactgcaaatgccttgaatgagcattaatttttaaaagttttacagagttttattagttttactactttatcttattggtctctacagacttgtgctggtgcaagtataggctgccaggcaattgcactatatttggctgcagattcaaacaggcagttctcttatttggttgacatttgtgaatgtgtaacacagagggaatacacatcacggtgacagataacactgccatggtaaaagtactcatgtttccccttttggaaaaaaagttagcttttagtgtttttcaaagttttcaaaagtgcccattttatgctgctgtgtggtttgttagatctaaatgatttttaaacttttctcatagaaagttaactttggcaataaacattttttaaggtccctcctttcctccccctaacagtatagttttctagatgagatttcggtatgattttatcagctatttcttatagaccatgatctggcaatttctgaaactggtcagaaaaaacatatatttccatgcctttttaaataataatttttatcagttttatagtcacagttggtgtcctgttactttgttttaaaataagctagaaccaggatgcttacttattggatgggctttgccaatccttaaggtacatttttgagtgatgctgtaagctgcaaaaatgtgccagtatttcaaactttcttcctgggaaacaaggtcactcgcacagttacttgcagtgcaaatttttgcttctctttcaacctgaagcatacgtggtggggtttccttccttttattaaactaaaactgcataggaaaagttatttcttaatagattctggattccactcacaaggagacgggttcctttcttgtccaaacaaagggagctgatgtaaagcaagcatcagtgaaaatataggggaaaatgtgttttgtatttagtgtagataacactttgtgaatggacaacagttacatagagtcttggtagttccagccagtgtttccccacccagattttactgactcctaattctatatcctggaacctcaagagtgaaagctggaaaaggggataaaagcctggagccacactccatatggctgaggaaggtgctggggaagaaacagatgtagtaaaactacttgccacctctgtttggaaactgctggtggggaaacactgtatgtgatggtgaagggtgtttgcttgaactcattaggtacaaatctcttaaactagccttctcaaaatcccagcaatataaagcactgtttttcttcagtcttttacacaagcttgtaaatacattgaagtttacatttccttgtgctgttgatcagcggcgccaccagtgtttgctgagactggtctttgagagttttataatgtatattaagttctaccgctcgacaagatgagcacagacgtccttattctctgcataaagtgggatgctagcaggctctatcattgaggcacaacacaggcaggaacaaagagatggcagacgttaatttcttaaaatttcccttttttgcatatacctcacttggctactacaaatcaggacatgttggtgagggactggctgctgcttttattcacacttgttcagggagagcacagaaaatacccttcagcatggccaccctggacccaagaggaggaaaaacagcttttttaggagaaggaggactcactagagtccaggatgggaagaactcttggtggctggctggccggccagcgggtgccacatctgcacttgagtgcctgggcctgcttattaataacgcacagaaacagagtcacttctcaccatctcctggcatgtatggatcagggagcgaggtggagtgccaggaagccctgataacttgcctgcagttgggctctctgctcagtcttcaactatacagttcgtacaacctcagtcagtcctcgtacactggggcaaaccagtgtggtggtggaggaaaagccgggaggcctatttttataggaaaacagtaccttgaagggtgcaatgctgtcgttttggatgaaaggaaataaatatggaagaagtaggattgttgggaaaaacttgctgaggggtgtaatcactgcagaatcagagttgcagcaatatcattgcaagcaagtctgaatgcatagttttattaatttggcttggcaatttgtgtaacccctccagactgactgaactgttatgcatgtgtcaatcaatgggaggtgtgcattctctagaagtagatttaggctgtttggttttttaaaatgcagcctgtttaggcactgtcatgtggtgtgaaagatttcagtgtagcaatgctaaagagttatggtttgaggataaggtgcagggacattaagagagtggtgaagatacaaatgttgaaaaaattatgttttgttgttgttagcttgaaccttgagcagttttataaagtgaactagatggcttaatgtagccagcattctgggcaaacagaatataaactcattcttactgtaaattctatttgctgcttccaaaggtgataatttacaagcagacatgttctatatggtctgtattttaggatctggtgcccagcctatatcaaagcttacctacctggctcagctacctacccttcctatgggaaaagacaattcattgttgaggaaggcttaccctctctgacctgccctagcgaccctttgtggatgtgctataagattttcctgctcaatagcaaagtggtagctctgctttcattttaagaaagtggaggctgagggcattgaatcaatactgcttcaactccaaagatttttccttgtaaaattaaagggaagatcttgttattaatgaaccattttcttatcccttgctattgacatattcatgctctttatatgtctagtggctgaaaatgtttgcatttgttcatttgactaatggtgtaatttttgtttttatattgttagacctgtaatgttttaaaatgtattttattaaatttgaactggatgtatgtctctagcaatacgaggtactttctaaactattattaagggaggggcagtgtcctcatgtggagataagatgaaggttgtttaaagttggcatttttttttttttggcctgcagggatattctgtgttcacgtgtccaaaaaagaataaattggcattcttgtgccacaagttgtt

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