LOCUS       XM_034436040            3908 bp    mRNA    linear   VRT 03-NOV-2023
DEFINITION  PREDICTED: Pantherophis guttatus argonaute RISC catalytic component
            2 (AGO2), mRNA.
ACCESSION   XM_034436040
VERSION     XM_034436040.1
DBLINK      BioProject: PRJNA1031231
KEYWORDS    RefSeq.
SOURCE      Pantherophis guttatus
  ORGANISM  Pantherophis guttatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Lepidosauria; Squamata; Bifurcata; Unidentata; Episquamata;
            Toxicofera; Serpentes; Colubroidea; Colubridae; Colubrinae;
            Pantherophis.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_026844077) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_029531705.1-RS_2023_10
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.2
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 10/30/2023
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3908
                     /organism="Pantherophis guttatus"
                     /mol_type="mRNA"
                     /isolate="1"
                     /db_xref="taxon:94885"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="blood"
     gene            1..3908
                     /gene="AGO2"
                     /note="argonaute RISC catalytic component 2; Derived by
                     automated computational analysis using gene prediction
                     method: Gnomon. Supporting evidence includes similarity
                     to: 43 Proteins"
                     /db_xref="GeneID:117676521"
     CDS             32..2638
                     /gene="AGO2"
                     /codon_start=1
                     /product="protein argonaute-2"
                     /protein_id="XP_034291931.1"
                     /db_xref="GeneID:117676521"
                     /translation="

MYPAGGAAPGAAAGPVLTTPPSLPPPMQAYAFKPPPRPDFGTSGRTIKLQANFFEMDIPKMDIYHYELDIKPEKCPRRVNREIVEHMVQHFKAQIFGDRKPVFDGRKNLYTAMPLPIGRDKVELEVTLPGEGKDRIFKVAIKWMSVVSLQALHDALSGRLPSVPFETIQALDVVMRHLPSMRYTPVGRSFFTASEGCSNPLGGGREVWFGFHQSVRPSLWKMMLNIDVSATAFYKAQPVIEFVCEVLDFKSIEEQQKPLTDSQRVKFTKEIKGLKVEITHCGQMKRKYRVCNVTRRPASHQTFPLQQESGQTVECTVAQYFKDRHKLVLRYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIRATARSAPDRQEEISKLMRSASFNTDPFVREFGIMVKDEMTDVTGRVLQPPSILYGGRNKAIATPVQGVWDMRNKQFHTGIEIKVWAIACFAPQRQCTEVHLKTFTEQLRKISRDAGMPIQGQPCFCKYAQGADSVEPMFRHLKNTYSGLQLVVVILPGKTPVYAEVKRVGDTVLGMATQCVQMKNVQRTTPQTLSNLCLKINVKLGGVNNILLPQGRPPVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDAHPNRYCATVRVQQHRQEIIQDLAAMVRELLIQFYKSTRFKPTRIIFYRDGVSEGQFQQVLHHELLAIREACIKLEKDYQPGITFIVVQKRHHTRLFCTDKNERVGKSGNIPAGTTVDTKITHPSEFDFYLCSHAGIQGTSRPSHYHVLWDDNRFSSDELQILTYQLCHTYVRCTRSVSIPAPAYYAHLVAFRARYHLVDKEHDSAEGSHTSGQSNGRDHQALAKAVQVHQDTLRTMYFA"

     misc_feature    299..556
                     /gene="AGO2"
                     /note="N-terminal domain of argonaute; Region: ArgoN;
                     pfam16486"
                     /db_xref="CDD:435368"
     misc_feature    584..736
                     /gene="AGO2"
                     /note="Argonaute linker 1 domain; Region: ArgoL1;
                     pfam08699"
                     /db_xref="CDD:430160"
     misc_feature    737..1099
                     /gene="AGO2"
                     /note="PAZ domain, argonaute_like subfamily. Argonaute is
                     part of the RNA-induced silencing complex (RISC), and is
                     an endonuclease that plays a key role in the RNA
                     interference pathway. The PAZ domain has been named after
                     the proteins Piwi,Argonaut, and Zwille; Region:
                     PAZ_argonaute_like; cd02846"
                     /db_xref="CDD:239212"
     misc_feature    order(893..895,938..940,980..982,992..994,1046..1048,
                     1067..1069,1073..1075)
                     /gene="AGO2"
                     /note="nucleic acid-binding interface [nucleotide
                     binding]; other site"
                     /db_xref="CDD:239212"
     misc_feature    1232..2509
                     /gene="AGO2"
                     /note="PIWI domain, Argonaute-like subfamily. Argonaute is
                     the central component of the RNA-induced silencing complex
                     (RISC) and related complexes. The PIWI domain is the
                     C-terminal portion of Argonaute and consists of two
                     subdomains, one of which provides the...; Region:
                     Piwi_ago-like; cd04657"
                     /db_xref="CDD:240015"
     misc_feature    order(1643..1645,1655..1657,1691..1702,1709..1711,
                     1733..1735,1742..1744,1754..1756,1766..1768)
                     /gene="AGO2"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240015"
     misc_feature    order(1847..1849,1853..1855,2063..2065,2477..2479)
                     /gene="AGO2"
                     /note="active site"
                     /db_xref="CDD:240015"
     polyA_site      3908
                     /gene="AGO2"
                     /experiment="COORDINATES: polyA evidence [ECO:0006239]"
ORIGIN      

cgcctcccacgcagcccggcgctcggccgccatgtacccggccgggggagccgcccccggggccgccgcgggacccgttcttacaacacctccatcactgccacctccaatgcaggcatatgcatttaaacctccccctcgaccagattttggtacttcgggaagaacaatcaaactgcaagccaatttctttgaaatggacatccccaaaatggacatctatcattacgaattggatataaagcctgagaaatgtcctagaagagttaacagagaaatagttgagcacatggtgcagcactttaaagcccagatctttggagatcgcaagccagtatttgacgggagaaaaaatctctacaccgcaatgccgcttccaatagggagagataaagtggaattggaggtcacgttgccaggagaagggaaggaccgaatattcaaggtagccatcaagtggatgtcggttgtgagtcttcaggcattgcacgacgctctctccggtcgtcttccaagtgttcctttcgaaaccattcaagctcttgacgtggtgatgaggcacctgccctccatgagatatactccagtgggccgatctttttttactgcatccgaaggatgttcgaatcctctgggaggtggcagagaagtctggtttggattccatcagtcagtcaggccttccctgtggaaaatgatgctgaacattgatgtctctgcaacagcattttataaggcccagccagtgattgaatttgtatgtgaagttcttgatttcaaaagtattgaagaacaacagaaacctctgacagattcccaaagggttaagtttaccaaagaaattaaaggtcttaaggtggaaatcactcactgtggacaaatgaaaagaaaataccgagtatgcaacgttacgagaagaccagcaagtcaccaaacatttcctcttcagcaggagagtggccagactgttgaatgcactgtagctcaatattttaaggacaggcacaaattggtattacgctatcctcaccttccatgtttacaagttggacaggagcagaaacatacgtaccttcccttagaggtatgcaatatagttgcaggacagagatgtattaagaagctgacagacaatcaaacttctactatgattagagccacggcgagatcagccccagatcgtcaggaagagatcagcaaattgatgcgaagtgcaagttttaataccgaccctttcgttcgtgagtttggcatcatggtgaaagatgaaatgacggatgtcactgggagagtgttacagccaccttcaattctctacggaggccggaacaaagcaattgctaccccagtgcagggggtctgggatatgaggaacaaacagtttcatactggaattgaaatcaaggtctgggccattgcgtgtttcgctccccaacgccagtgcactgaagtccatcttaaaaccttcacggaacaactgaggaaaatatccagagatgcaggaatgcccatccaaggtcaaccatgcttctgtaaatatgcccagggagcagacagcgtggaacccatgttccgtcacctgaagaacacctattctggtctgcagcttgtggttgtcattctacccgggaaaactcctgtttatgccgaagtgaagcgtgtcggtgacaccgtgttgggaatggccacgcagtgtgtgcagatgaaaaatgtgcagaggactaccccccagaccctttccaacctatgcttaaagatcaatgtcaaactaggaggagttaataacattttattgccacaaggaaggcctccagtatttcagcagcctgttattttccttggtgcagacgtcactcatccaccagctggagatgggaaaaagccatctattgctgctgttgtaggcagcatggatgcccatccgaaccgctactgtgccactgtgagggtccagcagcaccgccaagaaataattcaagacttggctgccatggttagggagctgctgatccagttttacaaatcaactcgattcaagccaacgcggatcattttctatcgagacggtgtttccgaggggcagtttcaacaggttcttcatcatgaattgctggctatccgagaagcctgcattaaactagaaaaagattaccagccagggattaccttcattgtggtgcagaagaggcatcacaccaggctgttttgcaccgacaaaaatgaaagagttgggaaaagtggaaacattccagcaggtaccactgtggacacaaaaatcacccacccttcggagtttgacttctacctgtgtagtcatgctggtattcagggcacaagcagaccgtctcattaccatgttctctgggatgacaatcgcttctcttcagacgaactccagatcctcacttaccaactgtgtcatacctacgtccggtgtactcgatctgtttctatccctgcgccagcctactatgcacacctggtggccttcagagccaggtatcatttggtggataaagagcatgacagcgccgaagggagccacacttccgggcagagcaacggccgcgatcaccaagctctcgcgaaagctgtccaggtccaccaagatactttgcgcaccatgtactttgcttgacatctggccgtgttgaggaactcagttctgtgttggattcacaagagaccagctgcacttagaccaacagttgcccgagctaccgcgacagccagcaaaggagtgatgcatctttatttttaaatatttgaggggtggggaggggaggagggggctgatgatgatgatgatgattgtgattctatttgcaagaaagcttttcatccagaatttcagacttgagcgtagaaaaaaaaaattgcacagcttttaagacatgtggatatgccaaaatcaaaaagctgcttgtcgggagaaatgaaacaaacaaacaaacaaacaaaaattgaaaacccacgttttctaattaactagagctgtgatctcagggctaaaaacaacacttctcattttagttatgactttttcagatgctcttatttgtaataatctgaagaaatatccaattaagatccgattgaacgggcctgggaggaaaaaagaaatgaatttgcttttatttctttaaaaaaaaaaaagcactagtttggatgccagtgaagtctttgatcattctggatgcatttgtaaagtgattttgtaccgagatgcaaaaagagtgagcgaatcctttgctgtcttattgataagtctgtgaaggtctatgggcaaaaaaaaaaaaaaaatatgtatttttgacgttagttcttgaatgtctttcattgtgctgtttaaaattttttgtaggatgagttacggaattatttcagttacaaaaaagaagttttatctatactttttattaggtccactgaaagattaatgaagtgtgccactttttttaaaaaaaaagtttattttttcttaaaaaaaaaataataataatccattttgattactaatcatggagtttgtcctctaggactgtgacacttaattttccaaagcttttctaaagaatacgggtctgtggtgatacagtacaatctctttcactgttatgtttgaattaatgtaaaatttatatgtgtttcacagtattaacatgatagtatagatgctatttatatttatttactaaagagtgctcaacatagttctgtttaacatatcacaaattgaagaatgctttggagttttaaagtggagagcttttacgtggacgagagggatttgggtcgaattcaccaaccgtagcttttttgcaggttcccttgttcattttaacgaggtttctttattatatctccttgaagtgaacaaaggagaactggacctgatgtgttgtccc

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