LOCUS       XM_033422155            6665 bp    mRNA    linear   MAM 02-MAR-2023
DEFINITION  PREDICTED: Orcinus orca protein argonaute-4 (LOC101280127),
            transcript variant X2, mRNA.
ACCESSION   XM_033422155
VERSION     XM_033422155.2
DBLINK      BioProject: PRJNA854208
KEYWORDS    RefSeq.
SOURCE      Orcinus orca (killer whale)
  ORGANISM  Orcinus orca
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Laurasiatheria; Artiodactyla; Whippomorpha;
            Cetacea; Odontoceti; Delphinidae; Orcinus.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_064559) annotated using gene prediction method: Gnomon,
            supported by mRNA evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Aug 2, 2022 this sequence version replaced XM_033422155.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Updated annotation
            Annotation Name             :: GCF_937001465.1-RS_2023_02
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.1
            Annotation Method           :: Best-placed RefSeq; Gnomon;
                                           cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 02/27/2023
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6665
                     /organism="Orcinus orca"
                     /mol_type="mRNA"
                     /db_xref="taxon:9733"
                     /chromosome="1"
     gene            1..6665
                     /gene="LOC101280127"
                     /note="protein argonaute-4; Derived by automated
                     computational analysis using gene prediction method:
                     Gnomon. Supporting evidence includes similarity to: 1
                     mRNA, 12 Proteins"
                     /db_xref="GeneID:101280127"
     CDS             355..2739
                     /gene="LOC101280127"
                     /codon_start=1
                     /product="protein argonaute-4 isoform X2"
                     /protein_id="XP_033278046.1"
                     /db_xref="GeneID:101280127"
                     /translation="

MVRHFKMQVFGDRQPGYDGKRNMYTAHPLPIGRERVDMEVTLPGEGKDQTFKVSVQWVSVVSLQLLLEALAGHLNEVPDDSVQALDVITRHLPSMRYTPVGRSFFSPPEGYYHPLGGGREVWFGFHQSVRPAMWNMMLNIDVSATAFYRAQPIIEFMCEVLDIQNINEQTKPLTDSQRVKFTKEIRGLKVEVTHCGQMKRKYRVCNVTRRPASHQTFPLQLENGQAMECTVAQYFKQKYSLQLKYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIKATARSAPDRQEEISRLVKSNSMVGGPDPYLKEFGIVVHNEMTELTGRVLPAPMLQYGGRNKTVATPNQGVWDMRGKQFYAGIEIKVWAVACFAPQKQCREDLLKSFTDQLRKISKDAGMPIQGQPCFCKYAQGADSVEPMFKHLKMTYVGLQLIVVILPGKTPVYAEVKRVGDTLLGMATQCVQVKNVVKTSPQTLSNLCLKINAKLGGINNVLVPHQRPSVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDGHPSRYCATVRVQTSRQEISQELLYSQEVIQDLTNMVRELLIQFYKSTRFKPTRIIYYRGGVSEGQMKQVAWPELIAIRKACISLEEDYRPGITYIVVQKRHHTRLFCADKTERVGKSGNVPAGTTVDSTITHPSEFDFYLCSHAGIQGTSRPSHYQVLWDDNCFTADELQLLTYQLCHTYVRCTRSVSIPAPAYYARLVAFRARYHLVDKDHDSAEGSHVSGQSNGRDPQALAKAVQIHHDTQHTMYFA"

     misc_feature    364..618
                     /gene="LOC101280127"
                     /note="N-terminal domain of argonaute; Region: ArgoN;
                     pfam16486"
                     /db_xref="CDD:435368"
     misc_feature    649..801
                     /gene="LOC101280127"
                     /note="Argonaute linker 1 domain; Region: ArgoL1;
                     pfam08699"
                     /db_xref="CDD:430160"
     misc_feature    802..1164
                     /gene="LOC101280127"
                     /note="PAZ domain, argonaute_like subfamily. Argonaute is
                     part of the RNA-induced silencing complex (RISC), and is
                     an endonuclease that plays a key role in the RNA
                     interference pathway. The PAZ domain has been named after
                     the proteins Piwi,Argonaut, and Zwille; Region:
                     PAZ_argonaute_like; cd02846"
                     /db_xref="CDD:239212"
     misc_feature    order(958..960,1003..1005,1045..1047,1057..1059,
                     1111..1113,1132..1134,1138..1140)
                     /gene="LOC101280127"
                     /note="nucleic acid-binding interface [nucleotide
                     binding]; other site"
                     /db_xref="CDD:239212"
     misc_feature    1303..2610
                     /gene="LOC101280127"
                     /note="PIWI domain, Argonaute-like subfamily. Argonaute is
                     the central component of the RNA-induced silencing complex
                     (RISC) and related complexes. The PIWI domain is the
                     C-terminal portion of Argonaute and consists of two
                     subdomains, one of which provides the...; Region:
                     Piwi_ago-like; cd04657"
                     /db_xref="CDD:240015"
     misc_feature    order(1714..1716,1726..1728,1762..1773,1780..1782,
                     1804..1806,1813..1815,1825..1827,1837..1839)
                     /gene="LOC101280127"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240015"
     misc_feature    order(1918..1920,1924..1926,2164..2166,2578..2580)
                     /gene="LOC101280127"
                     /note="active site"
                     /db_xref="CDD:240015"
     polyA_site      6665
                     /gene="LOC101280127"
                     /experiment="COORDINATES: polyA evidence [ECO:0006239]"
ORIGIN      

gcgcgccccgcgccctctccccgcccgacgggcgcgcgcgctggctccggctcccgctcccgctcccgctcccgctggcggcggcggcggggattgtttttgttgtcgctgaggccggaagagccgcgggagccgggtccctgtccccgggccgggcgccgccgccgccccttgcttagcgcccgcgtctccgcggcgcccccatagcgccaatattccggagatcaagcgttacgcggcggcggcggcggcggcggcggcggcggggcccggagcgggaggcgccggggaccgcggcgaggcggcccccgccgccgccatggaggctctgggacccgggacgtagtagatacaatggtgcggcacttcaagatgcaggtatttggtgatcggcagcctggctatgatggcaaaagaaacatgtacacagcacatccactgccaattggacgggagagggttgatatggaagtgacccttccaggtgagggtaaagaccagacctttaaagtgtctgttcagtgggtatcagttgtgagccttcagttgcttttagaagctttggccgggcacttgaatgaagtcccagatgactcagttcaagcacttgatgttatcacaagacaccttccctccatgaggtacactccagtgggtcgctcctttttctcacctcctgaaggttactatcaccctctgggagggggcagggaggtttggtttggctttcatcagtctgtgagacctgccatgtggaatatgatgctgaatattgatgtatctgcaactgctttctaccgggctcagcctatcattgagttcatgtgtgaggttttagacattcagaacatcaatgaacagaccaaacccctaacagactcccagcgtgtcaagtttaccaaagaaataagaggtcttaaagttgaggtgacccactgtggacagatgaaacgaaaatatcgagtgtgtaatgtgactaggcggccagccagtcatcagacttttcccttacagctagaaaatgggcaagcaatggaatgtacagtagctcaatattttaagcaaaagtatagtctgcaactgaaatacccccatcttccctgtctccaggtgggacaagaacaaaagcatacatacttgccacttgaggtttgtaatatagtggcaggacaacgatgtataaagaagctcacagacaatcagacttccacgatgatcaaagccacagcaagatctgctcctgacagacaagaagaaatcagtagactggtgaagagtaacagcatggtgggcggacctgatccatatcttaaagaatttggtattgttgtccacaatgaaatgactgagctcacaggcagggtacttccagcaccaatgctgcaatacggaggccggaataaaacggtagccacacccaaccagggtgtctgggacatgcgaggaaagcagttttatgctggcattgaaattaaagtttgggcagttgcttgttttgcgcctcagaaacaatgtagggaagatttactaaagagtttcactgaccagctccgtaaaatctctaaggatgcaggaatgcccatccagggtcagccatgtttttgcaagtatgcacaaggtgcagacagcgtggagcccatgtttaaacacctgaaaatgacatatgtgggcctacagctaatagtggttatcttgcctgggaagacgccagtatatgcggaggtgaaacgtgttggagataccctcctgggtatggctacacagtgtgtccaggtaaaaaatgtagtgaagacctcacctcaaaccctttccaacctttgcctgaagataaatgcaaagctcggaggaattaacaacgtgcttgtacctcatcaaaggccctcggtgttccagcagcctgtcatcttcctgggagcggatgtcacgcaccccccagcaggggatgggaagaagccttccattgctgctgtcgttggcagtatggacggccaccccagccggtactgtgccactgtgcgggtgcagacgtcccgccaggagatctcccaggagctcctctatagtcaggaggtaatccaggaccttactaacatggttcgagagctgctgatccagttctacaaatccacacgcttcaaacccactcggatcatctattaccgtggaggggtatccgagggacagatgaaacaggtagcttggccagaactaatagcaattcgaaaggcatgtattagtttggaagaagattaccggccaggaataacctatattgtggtgcagaaaagacatcacacacgactcttctgtgcagataaaacagagagggtggggaaaagtggcaatgtaccagcaggcactaccgtggatagcaccatcacacatccatctgagtttgacttttacctctgtagtcatgcaggaattcagggaaccagccgtccttcacattaccaggtcttgtgggatgacaactgcttcaccgcagatgagctccagttactaacttaccagctatgtcatacctatgtgcggtgcacacgctcagtctctattccagcccctgcctattatgcccgccttgtagcattcagagcaaggtatcatctggtggataaagatcatgacagtgcggaaggcagtcatgtgtcaggacaaagcaatggccgagatcctcaggccttggctaaagctgtgcagatccaccatgatacccagcacacgatgtattttgcctgagagtctcagaaaaagaactcaaccagtttggcacaccatgcaggctcaaaatgtttcaaacgcctactgcctctagagagagccaagttgacttcaggtggtcctctaccagcaactcagaatagttgcactgaatctatacttcgcagcactgtctgatgcatcaacaaaattgagccatttttttaaagtaatagatactcattgattatcttttctgatgcactggactgaatttttacctcatcatgcaaaggctaatcagcctgaactttctaaaggactttatgagaaaggtttctatggactattttgctagctgtggtgttcaccgcatccctctagtcttacaagagaagcttattccttttttctgtattcgtcgagtggggtatatgcataaatgggagagaaaaaccaatctacttcagttcagtgtaactatttaattgtgtgggtcaataggctttttaatggagatttctgacttcgccactgtaaatgcctttaacgagcattaacttttgaaagttttacagagttttattagttttactactttatgtattgatctctacagacttgtgctggtgcaagtacaggctgccaggcaattgcactatatttggctgcagattcagacaggcagttctcttatttggttgacttttgtgaatgtgtaacacatgtgatgcatgtcatggtgacagataacactgccatggtaaaaagtactcatgtttccccctctttgaaaaaaattagcttttagtatttttcagagttttcaaaaatgcccattttatgctgctgtgtggtttgttagatctaaatgattttttaacttttctcatagcaagttaactttggcaataaacattttttaaagtccctcctcttcctccccctgacagtgtagttttttagatgagatttcggtatgatttttatcagctatttcttacagatcataatctgacaatttttgaaactggtcagaaaaggcatatatttccatgccttttttttttttttttttttttttttgcggtacgcgggcctctcactgttgtggcctctcccgttgcggagcacaggctccggacgcgcaggctcagcggccatggctcacgggcccagccgctccgcggcatgtgggatcttcccagaccggggcacaaacctgtgtcccctgcattggcaggcggactctcaaccactgcgctaccagggaagccctccatgcctttttaaagaattctttttatctgttttatagtcacagttggtgtcctgtcactttgttttaaactaagctagaaccaggatgcttccttattgaataggctttgccagtccttaaggtaccttgagtgatgctgcaacttgcaaaaatgtaccagcatttaaaactttattccctgggaatcaaggttactcacacaattacttgtaatggaaattttttgcttctctttcaacctgaaatgtacttggtggggtttccttccttttattaaactaaaactgcatagaaaaggttgctttttcttaatagattctggattgcgttcacagggagagaggattcctttcttgtccaaacaaagggagttgatctaaagcaagcatcagttaaaatgtgggggaaaaggtattttgtatttagtgtagataatactttgtgaatggacaacagttacatagagtcttgatagttccagccagtgtttcccagcccaaattttactgacttggaattctctgtcctggaacctcaggagtgagagctgaaaggagggataaaaggcccagagccactccacatggctgaggaaggtgctgggggaagagatagatgcagtgtggtaaaacggttttgccacctctgtctggaaactgctggtgggggaaacactggatctaatggtgaaggatgttgcttgaactcattagctggtccaaatctgttaaactagccttctcaaaatcccagcaatataaagcactgtttttcttcagtcttgtaaacaagcttgtaaatacgttaaagtttacatttccatgtgctgttgatcagcagcgccaccagtgtttgctgagactggtctcagagttttataatgtatatggagtcctaaagctcattaagatacaagtgcagacgtccttgttctcttcatagagtgggatgaaagcaagctctgtaactgaggcacaacaaaggcaggagtgaagaagatggcagatgttaatttcttaagattttccttttttgcaaatacctcacttggacacacaaatcaggacacgttggtgaggggctggctgctgctttttattcacacttgttcagggagagcacagaaaatacccttcagcttggccaccctggatacaagaggaggaaaaaccgaagtttcctctaggaaaaggatgacttgctggagtccaggatgtgaagaactcttggtggctggctggctggccagtgggcaccccatctgcacttgagtgcctcggacttgcttgttaacaatgcacagaagaagcgtcacttctcaccatctcctggcatgtatggagcagggagcaaggcggagtgccaggaagccctgacgtgcttgcagacgggctatacactcgaccttgaactgtagtttgtaagacctcagtcagtcctagtacactggggcaaatcagtgtagtggtggaggaaaagccgggaggcctatttttataggaaaacagtacctttgaaggtttaatgctgccgttttggacaaaaggaaagaaatatagaagaattaggattgttggaaaaaacttgctgagcggtgaatctttaagaggtaattactgcagaatccgagttgcagcaatactcattgcaagcaggtctgaatgcatagttttaattcggcttgacaatttgaacacgttaaccccttgggactgactgaatcattatgcatgtgtcaatcaatgggaggtgtgcattctctagaagtaggtgtaggctgtttggtttttttttaatgcagcctgttcaggcactgtcatgtggtgtgaaagatttaaatgtagcaatgctaaagagttaacaattttgggataaggcacggggacattaaaagagtggtgaaaatccaaatgttgaaccaattatgttttgttgttgttgttagctgtagagtgaactagctgcccagctgtctgggcaggcagaacatattcattcttactgtaaattctatttgctgcttccaaaggtgatgatttacaagcagacatattctgtatggtctgtattttaggatctggtgcccagcctatatcagagcttacctacctggctcagctacctacccttcctgtgggaaaaggtaatccactgtttaaccaggctcaccctctccgccctgccctagcaaccctttgtggatgtgctgtaagattttcttgctcaatagcaaggtggtagctctgctttcattttaagaaagtggaggctgagggcattgtatcaatactgcttcaactccaaggatttttccttgtaaaattaaagggaagatcttgttatcgattaaccattttcgtaccccttgctattgacatattcatgctctttctatgtctagtagctgaaaaatgtttgcatttgttcatttgactaatggtgtcatttttgtttctatattgttaggcctgtaatgttctaaaatgtattttattaaatttggactggatgtatgtctctagcaatacgaggtactttctaaactattaagggaggggtggtatccccgagttgagataagatgatggttgtttaaattttgcaatttttttttggcctgcagggatattttgtgttcatgtgtccataaaagaataaattggcattcttgtgccaaacattg

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