LOCUS       XM_027996166            5307 bp    mRNA    linear   INV 22-AUG-2022
DEFINITION  PREDICTED: Aphis gossypii protein argonaute-2 (LOC114131047),
            transcript variant X1, mRNA.
ACCESSION   XM_027996166
VERSION     XM_027996166.2
DBLINK      BioProject: PRJNA869080
KEYWORDS    RefSeq.
SOURCE      Aphis gossypii (cotton aphid)
  ORGANISM  Aphis gossypii
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Paraneoptera; Hemiptera; Sternorrhyncha;
            Aphidomorpha; Aphidoidea; Aphididae; Aphidini; Aphis; Aphis.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_065532) annotated using gene prediction method: Gnomon,
            supported by mRNA and EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Aug 22, 2022 this sequence version replaced XM_027996166.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: Aphis gossypii Annotation Release
                                           101
            Annotation Version          :: 101
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.0
            Annotation Method           :: Best-placed RefSeq; Gnomon
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5307
                     /organism="Aphis gossypii"
                     /mol_type="mRNA"
                     /isolate="Hap1"
                     /isolation_source="Farm field"
                     /host="cotton"
                     /db_xref="taxon:80765"
                     /chromosome="3"
                     /tissue_type="Whole body"
                     /country="China: Anyang"
                     /collection_date="2016-05"
     gene            1..5307
                     /gene="LOC114131047"
                     /note="protein argonaute-2; Derived by automated
                     computational analysis using gene prediction method:
                     Gnomon. Supporting evidence includes similarity to: 1
                     mRNA, 4 ESTs, 71 Proteins"
                     /db_xref="GeneID:114131047"
     CDS             630..3377
                     /gene="LOC114131047"
                     /codon_start=1
                     /product="protein argonaute-2 isoform X1"
                     /protein_id="XP_027851967.1"
                     /db_xref="GeneID:114131047"
                     /translation="

MEVNANFVAPQEQINFKPVYENINGHFNGTPAAPVVAPVPVGVPGAMGVLQPQAPALQPQLVDVPVFNCPRRPNLGREGRPIVLRANHFQISMPRGYVHHYDINIQPDKCPRKVNREIIETMVHAYSKLFGNLRPVFDGRNNLYTRDPLPIGNDRMELEVTLPGEGKDRVFRVNIKWLAQVSLFALEEALEGRTRQIPYDAILALDVVMRHLPSMTYTPVGRSFFSSPEGYYHPLGGGREVWFGFHQSVRPSQWKMMLNIDVSATAFYKAQPVIEFMCEVLDIRDIGEQRKPLTDSQRVKFTKEIKGLKIEITHCGAMRRKYRVCNVTRRPAQMQSFPLQLENGQTVECTVAKYFLDKYKMKLRYPHLPCLQVGQEHKHTYLPLEVCNIVAGQRCIKKLTDMQTSTMIKATARSAPDREREINSLVRRADFNNDSYVQEFGLTISNSMMEVRGRVLPPPKLQYGGRTLPNQGGLNVQQTKQQALPNQGVWDMRGKQFFTGVEIRNWAIACFAPQRTVREDALRNFTTQLQKISSDAGMPIVGQPCFCKYATGPDQVEPMFRYLKSTFTGLQLVVVVLPGKTPVYAEVKRVGDTVLGMATQCVQAKNVNKTSPQTLSNLCLKINVKLGGINSILVPSIRPKVFNEPVIFLGADVTHPPAGDNKKPSIAAVVGSMDAHPSRYAATVRVQQHRQEIIQELSSMVRELLIMFYKSTGGYKPHRIILYRDGVSEGQFPHVLQHELTAIREACIKLEGDYKPGITFIIVQKRHHTRLFCADKKEQSGKSGNIPAGTTVDVGITHPTEFDFYLCSHQGIQGTSRPSHYHVLWDDNHFESDELQCLTYQLCHTYVRCTRSVSIPAPAYYAHLVAFRARYHLVEKEHDSGEGSHQSGCSEDRTPGAMARAITVHADTKKVMYFA"

     misc_feature    867..1256
                     /gene="LOC114131047"
                     /note="N-terminal domain of argonaute; Region: ArgoN;
                     pfam16486"
                     /db_xref="CDD:435368"
     misc_feature    1284..1436
                     /gene="LOC114131047"
                     /note="Argonaute linker 1 domain; Region: ArgoL1;
                     pfam08699"
                     /db_xref="CDD:430160"
     misc_feature    1437..1799
                     /gene="LOC114131047"
                     /note="PAZ domain, argonaute_like subfamily. Argonaute is
                     part of the RNA-induced silencing complex (RISC), and is
                     an endonuclease that plays a key role in the RNA
                     interference pathway. The PAZ domain has been named after
                     the proteins Piwi,Argonaut, and Zwille; Region:
                     PAZ_argonaute_like; cd02846"
                     /db_xref="CDD:239212"
     misc_feature    order(1593..1595,1638..1640,1680..1682,1692..1694,
                     1746..1748,1767..1769,1773..1775)
                     /gene="LOC114131047"
                     /note="nucleic acid-binding interface [nucleotide
                     binding]; other site"
                     /db_xref="CDD:239212"
     misc_feature    1935..3248
                     /gene="LOC114131047"
                     /note="PIWI domain, Argonaute-like subfamily. Argonaute is
                     the central component of the RNA-induced silencing complex
                     (RISC) and related complexes. The PIWI domain is the
                     C-terminal portion of Argonaute and consists of two
                     subdomains, one of which provides the...; Region:
                     Piwi_ago-like; cd04657"
                     /db_xref="CDD:240015"
     misc_feature    order(2379..2381,2391..2393,2427..2438,2445..2447,
                     2469..2471,2478..2480,2490..2492,2502..2504)
                     /gene="LOC114131047"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240015"
     misc_feature    order(2583..2585,2589..2591,2802..2804,3216..3218)
                     /gene="LOC114131047"
                     /note="active site"
                     /db_xref="CDD:240015"
     polyA_site      5307
                     /gene="LOC114131047"
                     /experiment="COORDINATES: polyA evidence [ECO:0006239]"
ORIGIN      

tgcgcgcgtgttgttgttgttgttgttattattattattattattgtcgtcatcgccgccgccgctccgttgtagtagccgtcgaacagtaccagcgaccggtccgttcgatcgcgttcgcgtctgtgcagtgaatctgttttcgattttcgctttcgtccgtcggcaaaaatacgaaatccgtgtcgtctcgactccacagcagtcactgtacttggacgtgcattcgtcgaaccgtcctttcggccgcgtcgcgaaccataggtataacccctcggtcagtacctataggtaaaggtaataatatatatatataatattatataacgtcctcactgcagtcgtccgatgtaacgccactgcaatatgggaccgaccggacggaggaaaaaaatcataacaacaacaataatatcgaatagtgtaccgctgccgaaaacgttgcgcacgcaagaccttgaccttgagacttcagttcagtagaccagcgccacccgccaccgccgccgataggtgtgcgtgcgtaaaggtagttgtgtgcttctacgctggttcttgttccactgcacggcgcccggtgtacccacatcggaagaagagaaaaaggaataacatagaataaataaatcatggaagttaatgctaattttgtagcacctcaggaacaaattaatttcaaacccgtttatgaaaacatcaatggacatttcaatggaacccctgcggcacctgttgtggccccagtaccagttggagttcctggagcaatgggcgttcttcaacctcaggctcctgcattgcaaccacaactagttgatgtaccagtatttaattgtccacgtcgaccaaatttgggacgagaaggccgaccaattgtgcttcgtgctaatcattttcaaatatcaatgccaagaggttatgttcatcattatgatatcaatattcaaccagataaatgtccacgaaaagttaatcgtgaaattattgaaactatggtccatgcgtacagtaaactttttggtaatcttcgacctgtatttgacggtcgaaataatttatatacgagagatccattgcctatcggaaatgatcgaatggaattagaagttacattgcctggtgaaggtaaagatagagtgttccgtgttaatattaaatggttagctcaagtatcattatttgctctggaagaagcattagaaggccgcacaagacagataccatatgatgctattttggctttggatgtagtcatgagacatcttccatctatgacttacacaccagtgggtcgatcatttttttcttctcctgaaggatattatcatccattaggtggtggtagagaagtttggtttggttttcaccagtctgtccgaccatctcaatggaaaatgatgcttaatattgatgtgtcagcaaccgctttttataaagcacaaccagtaatagaattcatgtgtgaagtattggatattagagatattggtgaacaaaggaaaccgttaacagattcacaaagagttaaatttacaaaggaaattaaaggtcttaaaatagagataacacattgtggagcaatgcgaagaaaatatagagtttgtaatgttacaagaagaccagctcaaatgcaatcttttccattgcaattggaaaatggtcagacagtagaatgcacagtagctaaatatttcttagacaaatacaagatgaaactacgttatccacatttaccatgtcttcaagttggacaggaacataaacatacatatcttcctttggaggtctgtaacatagtagcgggtcaacggtgtattaaaaaattaactgacatgcaaacatcaacgatgattaaggcaactgcacgcagtgctcctgatcgtgaacgagaaattaattccttagttcgcagagctgattttaataatgattcatatgtacaagaatttggtcttaccatatcaaatagcatgatggaagtgcgtggtcgtgtcttaccaccaccaaaattacaatatggtggacgaactctgcctaatcagggaggattaaatgttcaacaaactaaacaacaagcattgcccaatcaaggagtttgggatatgagaggcaaacagtttttcacaggtgtcgaaattcgcaattgggcaatagcttgttttgcaccacagagaactgttcgcgaagacgcgttaagaaactttacaacacaactccaaaagataagcagtgatgctggcatgccaatagttggtcaaccatgtttttgtaaatatgccactggacctgatcaagtagaacctatgttccgttacttaaaatcaacattcactgggttacaactcgtagttgtcgttttaccaggaaaaactccagtatatgctgaagtcaaaagagttggagacactgttctaggaatggctacacaatgtgttcaagcaaaaaatgttaacaaaacttcacctcaaacattgtctaatttatgtttaaagatcaatgtgaaattaggtggtatcaatagcattcttgtaccgagcatcagacctaaagtattcaatgaaccagtgatatttttgggtgcagatgtaactcacccaccagcaggtgataataaaaaaccatccattgcagcagtagttggatctatggatgctcatcctagtcgatatgctgctactgtacgtgtccaacaacacagacaagaaatcattcaagaacttagttctatggttagggaacttctaatcatgttttataaaagtactggtggttataagcctcatagaattatattataccgtgatggagtatcagaaggacaatttcctcatgtcttacaacatgaacttacagctattcgagaagcatgtattaagttagaaggtgattacaagccaggaatcacatttataattgttcaaaaacgtcatcatactagattgttctgtgccgataaaaaggaacaatccggaaaatctggaaatattccagcaggtacaacagttgatgttggaataacacatcctacagaatttgatttttacttatgtagtcatcagggaattcagggaacaagtcgcccaagtcattatcatgttttatgggatgacaatcattttgaatcagatgaattacaatgcttaacgtaccaattatgtcacacttatgtgagatgtacacgttctgtatctatacctgcacctgcctattatgctcacttagttgcatttagggctaggtatcacttagtcgaaaaagaacatgacagtggtgaaggatcacatcaatctggctgtagtgaagaccgaacacctggtgcgatggcacgagctattacagtacatgctgataccaagaaagttatgtactttgcttaaaataccaaaattaaaaaaaaaattatcgcgcaacatataagttaatattaggcataaattataatacgacctaccttgccaatcattgcctttaaatgtaactttagaccaaactaattattttttcgacctctgatgattaattttaagtttcattcttatattattttttttttttacattttgaattcccatatagtttttagacattttccaaagttcaattttgttgttgctagtatttgttttgttacctatttttcttttatttttatttctaatcaaaattagacatcacttgaatgcttccagtatcttagttatgcgaaaattctagtctaaccggtttatcgaaggaaatggttttgaatagcaacagcagtaatgaaaaatgcattcctcttttaaaatatataattattgtataataaatatatcgtattattgttaagtgtgtattatagtcataattagtaattgtttgtaatgtttgtattccaatcttcctgccaacttaatctctacttatctgattttatttcttcttatctcgagcttaaataattttaattgtgattaactttaattgtatttagttttccggttggttaattcccatttcttgttgatatagacatatgttaaatgtggtcctcaattaaaccggaaatgtggtaccagtcattgcttaatatctcaatattcttaatttatacatttcaataagttttattgtttaataacttccaacaattttccaaaatacttctattgtgataaatattttaaattattttatttttaaattatacatattggtattatacctgaagtatattattttgttttaataaaataaaaaagtgttttcatttggggttaagttgtaacaaacactaattgtacctgatacttaagaatatcataaaacttaatattagcagataagatttcagttcaatttcattttaggttattttgatcaaattttaagggaaccatattattttccctcaaaattattttatacttagttaaataaaacaaaattatatatttaatattaacttataatatttaataccttaaactatataacatttttatgttaattagaattaccatagtattataataatcaaacaatgcatataattttaaaatgtgcatttgattggtaccacaatattgctaaccctccgattttataatttgagtattaaatcaattaaattttaaaaatatttatatatatacatacatacatttacataattataataataattattattactattttatacaaaatattttatcaattttatcttagttagaaattaaaatttggttaatatttagttgaccggtatttaaaaagtatataagtatactttaattttaagtatactttttatgccttttgaattgctgttataagtataattcatatttataggaaaaataaaatgggataacttattatacccatttatattatacattattacgcttatattctgttagtttttttttcattaaatgatagatctattacttttttgcaaatcataaatatacgaggtatttgtattataaattataagaatatatattttaacattgtagtttaaattttgttttaagtaatttgattttaaaaatattgtttttaagattataatatatgcaacaaaaacgtataattgtcgtttaattaatatccatgtatctatctgataattcttaatgaaaacgtggtttaaggggttcaacagtatgcataattttaaaattattttgtatgaaaatagctgttaaaaatggttatacttgtatatatattacatattatttatataataataaatatgatatttattaccttttgcagtgcaattggtgaacataatatttaaactttgtaaaa

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