LOCUS       XM_026227975            3804 bp    mRNA    linear   VRT 18-JUN-2025
DEFINITION  PREDICTED: Carassius auratus protein argonaute-1 (LOC113059449),
            transcript variant X3, mRNA.
ACCESSION   XM_026227975
VERSION     XM_026227975.1
DBLINK      BioProject: PRJNA487739
KEYWORDS    RefSeq.
SOURCE      Carassius auratus (goldfish)
  ORGANISM  Carassius auratus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Ostariophysi;
            Cypriniformes; Cyprinidae; Cyprininae; Carassius.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_039283.1) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Updated annotation
            Annotation Name             :: GCF_003368295.1-RS_2025_06
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.4
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 06/18/2025
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3804
                     /organism="Carassius auratus"
                     /mol_type="mRNA"
                     /strain="Wakin"
                     /db_xref="taxon:7957"
                     /chromosome="41"
                     /tissue_type="muscle"
                     /dev_stage="juvenile"
     gene            1..3804
                     /gene="LOC113059449"
                     /note="protein argonaute-1; Derived by automated
                     computational analysis using gene prediction method:
                     Gnomon. Supporting evidence includes similarity to: 4
                     Proteins, and 100% coverage of the annotated genomic
                     feature by RNAseq alignments, including 20 samples with
                     support for all annotated introns"
                     /db_xref="GeneID:113059449"
     CDS             335..2683
                     /gene="LOC113059449"
                     /codon_start=1
                     /product="protein argonaute-1 isoform X3"
                     /protein_id="XP_026083760.1"
                     /db_xref="GeneID:113059449"
                     /translation="

MVQHFKPQLFGDRKPVYDGKKNIYTVLALPIGSEKVDFEVTIPGEGKDCIFKVSIRWLAKVSWRLLQETLVSGRLQVPLDSVQALDVAMRHLASMRYTPVGRSFFSPPEGYYHPLGGGREVWFGFHQSVRPAMWKMMLNIDVSATAFYKAQPVIEFMCEVLDIRNIDEQPKTLTDSQRVRFTKEIKGLKVEVTHCGQMKRKYRVCNVTRRPASHQTFPLQLESGQTVECTVAQYFKQKYSLQLKYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIKATARSAPDRQEEISRLMKNANFNLDPYIQEFGIKVKDDMAEVTGRVLPAPILQYGGRNRAIATPNQGVWDMRGKQFYNGIEIKVWAIACFAPQKQCREEVLKNFTDQLRKISKDAGMPIQGQPCFCKYAQGADSVEPMFRHLKNTYSGLQLIIVILPGKTPVYAEVKRVGDTLLGMATQCVQVKNVVKTSPQTLSNLCLKINVKLGGINNILVPHQRSAVFQQPVIFLGADVTHPPAGDGKKPSITAVVGSMDAHPSRYCATVRVQRPRQEIIEDLSYMVRELLIQFYKSTRFKPTRIIFYRDGVPEGQLPQILHYELLAIRDACIKLEKDYQPGITYVVVQKRHHTRLFCADKSERIGKSGNIPAGTTVDTSITHPFEFDFYLCSHAGIQGTSRPSHYYVLWDDNRFTADELQILTYQLCHTYVRCTRSVSIPAPAYYARLVAFRARYHLVDKEHDSGEGSHVSGQSNGRDPQALAKAVQIHHDTLRTMYFA"

     misc_feature    377..601
                     /gene="LOC113059449"
                     /note="N-terminal domain of argonaute; Region: ArgoN;
                     pfam16486"
                     /db_xref="CDD:465134"
     misc_feature    629..781
                     /gene="LOC113059449"
                     /note="Argonaute linker 1 domain; Region: ArgoL1;
                     pfam08699"
                     /db_xref="CDD:462567"
     misc_feature    782..1144
                     /gene="LOC113059449"
                     /note="PAZ domain, argonaute_like subfamily. Argonaute is
                     part of the RNA-induced silencing complex (RISC), and is
                     an endonuclease that plays a key role in the RNA
                     interference pathway. The PAZ domain has been named after
                     the proteins Piwi,Argonaut, and Zwille; Region:
                     PAZ_argonaute_like; cd02846"
                     /db_xref="CDD:239212"
     misc_feature    order(938..940,983..985,1025..1027,1037..1039,1091..1093,
                     1112..1114,1118..1120)
                     /gene="LOC113059449"
                     /note="nucleic acid-binding interface [nucleotide
                     binding]; other site"
                     /db_xref="CDD:239212"
     misc_feature    1277..2554
                     /gene="LOC113059449"
                     /note="PIWI domain, Argonaute-like subfamily. Argonaute is
                     the central component of the RNA-induced silencing complex
                     (RISC) and related complexes. The PIWI domain is the
                     C-terminal portion of Argonaute and consists of two
                     subdomains, one of which provides the...; Region:
                     Piwi_ago-like; cd04657"
                     /db_xref="CDD:240015"
     misc_feature    order(1688..1690,1700..1702,1736..1747,1754..1756,
                     1778..1780,1787..1789,1799..1801,1811..1813)
                     /gene="LOC113059449"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240015"
     misc_feature    order(1892..1894,1898..1900,2108..2110,2522..2524)
                     /gene="LOC113059449"
                     /note="active site"
                     /db_xref="CDD:240015"
ORIGIN      

cccgcaggccgggcattggcaccgttggcaaacccatcaagctgctcgccaactactttgaggtggaaatcccaaaaatggatgtgttccactatgaagtggacatcaaacctgataaatgtcctcgacgggtcaacagtttctgaaaagaacgaggcaggagaggcttctgtgagggtctcaagagtctctacccacagtatatataccacaacatccattgaggcttcactggaagtttaccagctgtcttatcttaatgcagaagtactaaagagcgctccgtgcatatggtctgctaaatgtgactcttcgcagggaggttgtggagtacatggtgcagcatttcaaaccgcagctgtttggcgaccgaaagccagtgtatgatgggaagaagaatatctacactgttttagcgctgcctataggcagtgaaaaggttgatttcgaggtgaccatccccggcgaggggaaggattgcatctttaaagtgtccattcgctggctggccaaagtgtcatggcggctgctgcaggagacgctggtcagcggacgtctacaggtcccgctggactctgtccaggccctcgacgtggccatgagacacctggcctccatgaggtacactcccgtgggacgctccttcttctcccctcctgaaggctactatcatcctctgggtggaggcagagaggtctggttcggcttccatcagtctgttcggccggccatgtggaagatgatgctcaacattgacgtgtctgcaacagccttttataaagctcagcctgtcattgagttcatgtgtgaagttctggacatccgtaacatcgacgagcagccgaagacgctcaccgactcgcagagggttcgcttcaccaaggagatcaaaggtctgaaggtggaggttacacactgtggtcaaatgaaaagaaagtaccgcgtctgtaacgtcacacgacgtcctgctagccaccaaacgtttcctctgcagcttgagagcggacagacggtggaatgtaccgtggctcaatacttcaagcagaagtacagcctgcagctcaagtatcctcacctgccgtgtctccaggtgggccaggagcagaaacacacctacctgcccctggaggtgtgtaatattgtagcagggcagcggtgcatcaagaaactgacagataatcagacgtccaccatgatcaaagccacagcgagatcagctccagaccgacaggaggagatcagcagactgatgaagaatgctaactttaatctggatccctacattcaagagtttgggatcaaggtgaaggacgacatggccgaggtgacgggccgagtgcttcccgctcccatcctgcagtacggcggtcggaatcgtgccattgcgactcctaaccagggtgtgtgggatatgaggggaaagcagttctacaacggcatcgagatcaaagtgtgggccatcgcttgcttcgccccgcagaaacagtgtcgggaagaggtgctcaagaacttcacggaccagctgcgtaagatctcgaaggacgctgggatgccgatccagggtcagccgtgtttctgtaagtacgcacagggagccgacagcgtggagcccatgttcagacacctgaagaacacctactccggcctgcagctcatcatcgtcatcctgcccggaaaaacccccgtctacgccgaggtcaagcgtgtgggagacactcttctaggaatggccactcagtgtgttcaggtgaagaacgtggtgaaaacctctcctcagacgctctccaacctctgcctcaagatcaacgtcaaactcggcggcatcaacaacatcctggtgccgcatcaacgatcggcagtgttccagcagccggtgatcttcctcggagcagacgtcactcacccgccggctggtgatgggaagaagccgtctattactgcggtggtgggcagcatggacgctcatcccagcagatactgcgctacagttcgagttcagcgacccagacaggagatcattgaagacctgtcctacatggtgcgagagttactcatccagttctacaagtccactcgcttcaaacccaccagaatcatcttctacagggacggcgtgccagaaggacagttaccacaaatcctgcactacgagctgttggccatcagagacgcctgcatcaagctggagaaggactaccagccgggcatcacctacgtagtagtgcagaaacgccaccacactcgcctcttctgcgccgataaatctgaaagaatcgggaagagcgggaacatcccagcaggaactacggtggacaccagcatcacgcatccgttcgagtttgacttctacctgtgcagtcatgcaggtattcagggcaccagccgaccctcgcactactacgtcctgtgggacgacaaccgcttcacagcggacgagctgcagatcctcacctaccagctgtgccacacatacgtgcgctgcacccgctccgtctccatcccagcgcctgcgtactacgcccgactcgtggctttccgtgcgcgataccatctggtggataaagagcacgacagcggggagggcagtcacgtctctggacagagcaacggtcgagatcctcaggcgctggccaaagccgtgcagattcaccacgacacactgaggaccatgtactttgcctgagcagatctttaccacacgctggaccatcacacacccctcgaaaccgttcccggggtgctaccagtcctacggaggctcacactttgtgttttgttgaatccggttgacctatgctctctttttttattatttaattgtgtattattgtatactaccttcacactccctcatgtacggatgtatgcaatacgggaaggaacgcagctgtcgttttaatacgccaacacaaagctgcctcttcacacgacgccagacttcaaagcaactgttgttacttcctcgttatttttcttgttgtggaattctcgtttgaaatgttcctttttgtgaatgacttggtacattagcacttcgttttagttaaaaacacaggcccagcgtttacatgtcgcagctggagatcgactgtagcgtaagacgcagatgcaggtgtctttaggatcggcgttagttaaagagtggccatattagcataacgagagatggaccactgagtgctcactgcatttgcactctcacatcagcaagcgtaacaccgaaaccagcattatgcaccagccaaactccatgcaactgctgaaaagagcccgcatctgtaaaacatgcgttttactgcacttgaaaaggatttttttttacttccaatgcacataactctttctgaactcacaggaatagttcacccgaaaaaaaattcactcttgtgtcattgcaaaccctggattttttttcttcggtgaaacagaacgagctgttttcagtgcaacactagatagagggtgatttttactgtaaagcgccatatacatgtataaaactagcctgcatgacttgcatattatagcccagtctttctgaaatcatattttagatttgtgtaagaaaaagatgcaaatttaagttattgttcactattatttacttttagctttttgtttttttaaatgcgtctgtaaaagagatttgagagctgcagtgaaggagacgataatcagttaataatcatgtcatttttggtctgttccttacataaatgtattatgtgaatacagaagacttggaaaagcgtattttcatggcaatataaattaccatccaactttgttgtacagaaaacaaaa

//