LOCUS       XM_026152486            3881 bp    mRNA    linear   VRT 21-JUL-2025
DEFINITION  PREDICTED: Astatotilapia calliptera NRDE-2, necessary for RNA
            interference, domain containing (nrde2), mRNA.
ACCESSION   XM_026152486
VERSION     XM_026152486.1
DBLINK      BioProject: PRJNA488811
KEYWORDS    RefSeq.
SOURCE      Astatotilapia calliptera (eastern happy)
  ORGANISM  Astatotilapia calliptera
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Ovalentaria; Cichlomorphae; Cichliformes;
            Cichlidae; African cichlids; Pseudocrenilabrinae; Haplochromini;
            Astatotilapia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_039320.1) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Updated annotation
            Annotation Name             :: GCF_900246225.1-RS_2025_07
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.4
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 07/17/2025
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3881
                     /organism="Astatotilapia calliptera"
                     /mol_type="mRNA"
                     /db_xref="taxon:8154"
                     /chromosome="19"
     gene            1..3881
                     /gene="nrde2"
                     /note="NRDE-2, necessary for RNA interference, domain
                     containing; Derived by automated computational analysis
                     using gene prediction method: Gnomon. Supporting evidence
                     includes similarity to: 3 Proteins, and 100% coverage of
                     the annotated genomic feature by RNAseq alignments,
                     including 14 samples with support for all annotated
                     introns"
                     /db_xref="GeneID:113012351"
     CDS             50..3610
                     /gene="nrde2"
                     /codon_start=1
                     /product="protein NRDE2 homolog"
                     /protein_id="XP_026008271.1"
                     /db_xref="GeneID:113012351"
                     /translation="

MALFPAFAEEASNKVRDSSTEFDWLKNKSFQTGDALSLHGRFLEKTSEEQNKKEPSVSRDVSSEEDREDGVAPRKKKRKKKQKKKHKKKSGRYSHSSASDSETIYPSDLKKEQEAKRQEAPQLASHFSWLDDLQLPTEQLFCVDRKADPANWTYKSLYRGDVARYRRKGSSALGLDFHKQGVSWEDSEVTNKKKKKGGDGKKAADRYYSTVSRQLLKAAPALPALPASFDGSDGTSSAIFLPLCDDEADNKEGEKAHIGQTSSVNPLGVYDSSTALWLQGKGQQDQQKKDAHTEEGTALLGGRTEDFNRRLREQPADTQLWIEFIRYQDEASAAVFKGEEEQQGSDAAERRKSSYRAVLEKKLSIAERAVATNPLCIALQLERLRICQELWEPSALAKEWKKLVFLHPNSTPLWREYLLFTQSYFSSFTVSKVNAAYGKCLSTLSAVRDGSMVSHPALPGIEEDMLDIFMQQCHFLRQCGHSEKSTAMFQAMIDFTFFKPDSVRELSTKQQVDFFEPFWDSGEARIGELDARGWKAWMLQQERGGWLQPTAEEEEEEEEDEEEVKDRTQPRGTIWLDVESSREATDWLPWRPDKAKGQSEEDCEDPDRQVLFDDIGPSLICLSSPELHLRLLLRFLSFLGLPVDSVLSVSPGQPGLLLENLSLLTQGNAPQRPLTSYDLADGGINSVGHMTTLQGTRKWVGLGKQGEKFLTNLFNMLQPVLPPHHRAFLSLSWIQYEKLKVMRCLHGGNKKRLRSQGKSSKRVAKRLLKEPDNRSSLVLWREYGHLEWLLGNLDEARKVFSTATALGGTKGLSSLTLCDLCLLWSQLELEDGAKERGGQPADVTASPAVSVLTRLAEGTSASSTSSSLSTVSILKARKSYDQTLAASLSALDQNSKVQDDLMAEKLRLRGLVGCYALFQYLTVSVQAANTVYTQVREKMEELHRTLTAEQQLNSSKEGKLARADADSNRPCVSRVAAECEALAWQQAALLKYHNTVAVFPLVTLRQTLTSALPVWTTSVPLWSIYVQVENRYHTAGRARRFFHSVTRNCSSVVPRLFAIVAEQQRKQLVDAAQRSCLHDDPLSILPENGLSNRIRGLFENAIATETGAHCPLLWRMYIYFLVCEGKVDKATGIFYKALQNIPWCKGLYMDAAQLFPDHLQEFVDLLTEKELRVRLPLEELDILLED"

     misc_feature    470..784
                     /gene="nrde2"
                     /note="MTR4-interacting domain (MID) found in nuclear
                     exosome regulator NRDE2 and similar proteins; Region:
                     NRDE2_MID; cd22200"
                     /db_xref="CDD:412062"
     misc_feature    order(470..493,500..505,515..520,524..526,530..574,
                     581..589,593..601,620..622,644..649,659..664,668..673,
                     680..700,725..736,755..784)
                     /gene="nrde2"
                     /note="MTR4 binding site [polypeptide binding]; other
                     site"
                     /db_xref="CDD:412062"
     misc_feature    968..1927
                     /gene="nrde2"
                     /note="necessary for RNA interference; Region: NRDE-2;
                     pfam08424"
                     /db_xref="CDD:462472"
ORIGIN      

ccgttgtgacgtcatatccctgcgccctgtttctaagtgacgcagcatcatggcgctgtttccagcgtttgcagaagaggcgagcaacaaagttagagattcttccacagagtttgattggctgaagaataagagttttcagacaggagatgcgctctctcttcacggtcgatttctagagaagaccagcgaggagcaaaacaagaaggaaccaagtgtcagcagagatgtcagctctgaagaggacagagaggatggtgtggcacccagaaagaagaaaaggaagaagaaacagaagaaaaagcacaaaaaaaagagtgggaggtattcgcacagcagcgcatctgactctgaaaccatctacccgagtgatctcaaaaaggagcaagaagccaaaaggcaggaggctcctcagttagcgagccatttctcttggttggatgacctccagttgcccacagagcagctgttttgtgtggaccgaaaagcagaccccgccaactggacttacaagtctctgtacagaggagacgtagccaggtatagaaggaaaggcagctcggctctgggtttggactttcacaagcagggggtcagctgggaggactcagaggtgactaataagaagaagaagaaaggcggcgacgggaagaaggcagcggacagatactactctacagtcagtcgacagctgctgaaggcagcgcctgctcttcctgcattaccagcgagttttgatggtagcgatggcaccagttctgcaatcttcctccctctgtgtgatgatgaggcagacaataaagaaggggagaaagctcacatagggcagacgtcatcggtgaatcccctcggtgtgtatgactcctcgacggccctctggctgcaggggaaaggacagcaagaccagcaaaagaaggacgctcacacagaggagggcactgcacttttgggtggcaggaccgaagatttcaacaggcggctgagagagcagccagcagacacacagctgtggatagagttcatacgatatcaggatgaggcgagtgcagctgtgttcaaaggcgaggaggagcagcagggcagtgatgctgctgagcgccgcaagtcttcctacagggcagtgctggagaaaaagctaagcattgcagagcgcgccgtggccaccaaccccctctgcatagctctgcaactcgagagactccgcatctgccaggagctctgggagccctctgctctggctaaagagtggaagaaactggtgtttctccacccaaacagcacccccctgtggagggagtatctgctcttcacccagagctatttcagcagcttcaccgtgtccaaggtcaacgctgcgtacggcaagtgtctgagcacgctcagtgctgtccgagatggcagcatggtgtctcacccggctctgccaggaatagaggaggatatgttggatatcttcatgcagcagtgtcactttctgcgtcagtgtggtcattcggagaagtcgactgccatgtttcaggcaatgatcgactttactttcttcaagcctgacagcgtgcgagagctgtccactaagcagcaggttgacttctttgagccgttctgggacagcggcgaagcaaggatcggggagctggacgccagaggctggaaagcctggatgctccaacaagagcgaggaggttggctacaacccactgcagaagaggaggaagaagaggaagaggatgaagaggaggtgaaggacaggacccagcccagaggtaccatctggctggatgtggagtcatctcgtgaagccacagactggttgccctggagacccgacaaagccaagggccagtcagaggaggactgtgaggatcctgacagacaggtgttgtttgatgacattggcccatcactgatttgtttgtcttcgccagagctccatctccgtctgcttctccgtttcttgagctttttgggtttgcctgtggactcagtgctctcggtttccccaggtcagcctggcttgctgctggagaacctttctctgctcactcaaggtaatgctccccagcgtcctctgacctcctatgatttagctgatggtggcattaactctgtcggtcacatgaccactctccaaggaacacggaagtgggtggggcttgggaaacagggtgagaagttcctcacgaacttgttcaatatgcttcagcctgtgcttccgccccaccaccgagccttcctgtcactcagctggatccagtacgagaaactcaaggtcatgcgctgcttgcacggcggaaataaaaagcgccttcgttctcaggggaagagcagcaagcgagttgccaagcggctgctcaaagaaccagacaatcgctcatcattagtgttgtggcgggagtacggccacctggaatggctactgggcaacctggatgaggcccgcaaagtgttctccactgcaacagcgcttgggggaaccaaagggctgagcagtctcactctgtgtgatctctgcctgctgtggtcccagctggagttggaggatggagcgaaagagcgagggggtcaaccggctgatgtaacagcatccccggctgtgtctgtactgacccggctagcagaaggaacctctgcatcgtccacttcatcctccctctccacggtttccatcctgaaagccagaaaatcgtacgaccagactctggctgccagtttatcagcactggaccaaaacagtaaagtccaagacgacctgatggcagagaagctgaggttgagaggtctggttggctgctacgctcttttccagtacctcacggtgagcgtgcaagcagccaacacagtctacacccaggttagagagaagatggaggaactacaccgcacgttaacagctgaacagcagctgaacagcagcaaggagggtaagctcgcaagagctgacgctgactcaaacaggccctgtgtcagcagagtagctgctgagtgtgaggcgttggcctggcagcaggcggcactgctcaagtaccacaacaccgtcgctgtgtttcctctggtgacactgaggcaaacgctgacatcggccctcccagtctggaccaccagcgtccctctgtggagcatttatgtacaggtggagaaccgttaccataccgctggccgtgcacgccggttttttcactctgtaaccagaaactgcagcagcgtggtgccgcgtctctttgccattgttgctgaacagcaaaggaagcagctggtagatgcagctcagagatcgtgtttacatgatgatcccttatccatcctgccggagaatggcctcagcaaccgcatccgtggactgtttgaaaacgccatagcaacagaaactggggctcattgtcctttactttggagaatgtacatctacttcctggtgtgtgaaggaaaagtggataaagccacaggaatcttctacaaggccttacagaatatcccctggtgtaagggtttgtatatggacgcagcgcagctgttccccgatcacctgcaggagtttgtagacctgttgacagagaaagaactgcgagtgcgactgcctttagaagaattggacatactgctggaagactgagcacacacacaatcttaattcaagtgtgtgttcaaattgtcaaatttgatgggaagaaaaggacaagcacaaaggcaaactcgacacatatacagctgcagtaatttgagaaaagagctgggttttgtttgtttggtttttttgtataaagtgacacaaaatgctttaattgccacaacaaaccactaaaactgtatttcttttacttgatattgtcaataaatacaagattgatctatgtgacatgccttaataaaacatgtaaaattta

//