LOCUS       XM_018327384            4254 bp    mRNA    linear   PLN 28-AUG-2017
DEFINITION  Purpureocillium lilacinum RNA interference and silencing protein
            (VFPFJ_10316), partial mRNA.
ACCESSION   XM_018327384
VERSION     XM_018327384.1
DBLINK      BioProject: PRJNA342689
            BioSample: SAMN04404347
KEYWORDS    RefSeq.
SOURCE      Purpureocillium lilacinum
  ORGANISM  Purpureocillium lilacinum
            Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina;
            Sordariomycetes; Hypocreomycetidae; Hypocreales;
            Ophiocordycipitaceae; Purpureocillium.
REFERENCE   1  (bases 1 to 4254)
  AUTHORS   Wang,G., Liu,Z., Lin,R., Li,E., Mao,Z., Ling,J., Yang,Y., Yin,W.B.
            and Xie,B.
  TITLE     Biosynthesis of Antibiotic Leucinostatins in Bio-control Fungus
            Purpureocillium lilacinum and Their Inhibition on Phytophthora
            Revealed by Genome Mining
  JOURNAL   PLoS Pathog. 12 (7), E1005685 (2016)
   PUBMED   27416025
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4254)
  CONSRTM   NCBI Genome Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-AUG-2017) National Center for Biotechnology
            Information, NIH, Bethesda, MD 20894, USA
REFERENCE   3  (bases 1 to 4254)
  AUTHORS   Wang,G., Liu,Z., Lin,R., Li,E., Mao,Z., Ling,J., Yin,W. and Xie,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-FEB-2016) Institute of Vegetables and Flowers,
            Chinese Academy of Agricultural Sciences, Zhongguancun South St.,
            Haidian District, Beijing 100081, China
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. This record is derived from an annotated genomic
            sequence (NW_017264041).
            COMPLETENESS: incomplete on both ends.
FEATURES             Location/Qualifiers
     source          1..4254
                     /organism="Purpureocillium lilacinum"
                     /mol_type="mRNA"
                     /isolate="PLFJ-1"
                     /isolation_source="soil"
                     /host="plant-parasitic nematode"
                     /db_xref="taxon:33203"
                     /chromosome="Unknown"
                     /country="China: Fujian Province Fuding City"
                     /lat_lon="27.34 N 120.30 E"
                     /collection_date="2009-10"
     gene            <1..>4254
                     /locus_tag="VFPFJ_10316"
                     /db_xref="GeneID:28892433"
     CDS             1..4254
                     /locus_tag="VFPFJ_10316"
                     /note="similar to Coccidioides immitis RS XP_001241132.1"
                     /codon_start=1
                     /product="RNA interference and silencing protein"
                     /protein_id="XP_018174080.1"
                     /db_xref="GeneID:28892433"
                     /translation="

MDFLLGLLTYLAWGRSLAFDGLRMPRLMIHAILLVRQMRLDQAVPERGHITEPVAALFAGDSGGGGNKFGYAGQLTPAQFLERQRAVLGCFVLSSAVSAYCGHVDALRWTTHMEQGLAAISANKACPTDEAFALQVRLQLLAYQSNQLLRQHDLNPGSSVSEASTSPALMSLETHLEQLHKLQPGVSSGLLYGKLAMAHAHSVELCLNLSLYSLASTVPVMLRQFGRMTRSGPTSGLSEGTTDSRTARHEQTQRLWQCTRSGKACVSAMLGLSPSELANVSFIQWEQLTRSVAVLHHLATELEDPIWDRQAMRADADVPGLFEGAVNKLRLVAEWRGEKDPGDAFTGLACKLCKWCSDGYGVVVERATADAYRSFAIAIQSLLTGSRHAGHLSLAPFDLNGSGSGYPNTKRDLTTASDKVRGTTSGCPTAAASTNAGAVVLVVEEALAVVEAIAVEAALAAEERLVVASAAEVLQARQQCSEDAIQASRRALSLDSLQLKAAFPTRPGYGTKGKAIVLWSNYVTFAVAPKLVLYRYDMSIQPSAPKRKTTQIIRLLLQSSELNRVRDEVVTDFRSTLISRQEFPDQAVQITYREEDEDDPAENATVYTVTLQRTNVLSTSELIDYVTSTNVSANYDAKLPMIQAFNIFLNHYAKSTGSLATIGSSKTFAMDPAASDQFDLGGCLTAIRGFYASVRAATARVLVNVNVSHGAFYQEGPLDRFIMTFGKARGFYKLERILKKLRVTTNHLREKRNRNGKVIPRVKTIIALASPRDGQGLPHPPRVREHGAGSNGVEFWLDAQPAASSASSAGTKKGGKGSKAGPQKAPSEGHYISVYDFFLKTHNIKIADPSMPVVNVGTRDRPSYLPAQVCSVIPGQAAQTKLDPGQTQNMIRFAVRRPADNATSIVSQGLRTAGLSPATNPLLATFGVTVGTDLVTVHGRILATPRVLYKANQKAQMMAGGWNMVPQGGPALKFSTTMALRTWSCLYIEFPPEFYHDAHKFTMEGLTDVMNKFYAVLVGTGIAASTPMKPQRVRLRDEEDPALDQCLQRAANSLDLLFVVLPAGPIPLYNRIKYLGDVKYGIQTVCCVGKKLAKASGQDQYLRNEALKVNLKLGGSNQVVEPMRNDLIADGKTMVVGIDVTHPSPGSSSEAPSIAGMVASVDKSMGQWPAALRIQPQRRQEMVADLKDMLLSRLRLWHTKGKNASLPENILVYRDGVSEGQYQLVLNEELPLLRAACKEIYPTDAQKKGLPRMTVVIVGKRHHTRFYPSSEADMDRSGNTKPGTVVDRGVTEGKTWDFFLQAHAALQGTARPAHYVVVLDEIFRARHARSSAEAAASDELQELTQNMSYTFGRATRAVSICTPAYYADILCERARCYLSSLFEGSTNASVTDTAGGVGSASDDIKTHEKLKDTMFYI"

     misc_feature    1708..1947
                     /locus_tag="VFPFJ_10316"
                     /note="N-terminal domain of argonaute; Region: ArgoN;
                     pfam16486"
                     /db_xref="CDD:435368"
     misc_feature    1987..2142
                     /locus_tag="VFPFJ_10316"
                     /note="Argonaute linker 1 domain; Region: ArgoL1;
                     pfam08699"
                     /db_xref="CDD:430160"
     misc_feature    2143..2619
                     /locus_tag="VFPFJ_10316"
                     /note="PAZ domain, argonaute_like subfamily. Argonaute is
                     part of the RNA-induced silencing complex (RISC), and is
                     an endonuclease that plays a key role in the RNA
                     interference pathway. The PAZ domain has been named after
                     the proteins Piwi,Argonaut, and Zwille; Region:
                     PAZ_argonaute_like; cd02846"
                     /db_xref="CDD:239212"
     misc_feature    order(2266..2268,2383..2385,2500..2502,2512..2514,
                     2566..2568,2587..2589,2593..2595)
                     /locus_tag="VFPFJ_10316"
                     /note="nucleic acid-binding interface [nucleotide
                     binding]; other site"
                     /db_xref="CDD:239212"
     misc_feature    2761..4134
                     /locus_tag="VFPFJ_10316"
                     /note="PIWI domain, Argonaute-like subfamily. Argonaute is
                     the central component of the RNA-induced silencing complex
                     (RISC) and related complexes. The PIWI domain is the
                     C-terminal portion of Argonaute and consists of two
                     subdomains, one of which provides the...; Region:
                     Piwi_ago-like; cd04657"
                     /db_xref="CDD:240015"
     misc_feature    order(3205..3207,3217..3219,3253..3264,3271..3273,
                     3301..3303,3310..3312,3322..3324,3334..3336)
                     /locus_tag="VFPFJ_10316"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240015"
     misc_feature    order(3415..3417,3421..3423,3643..3645,4102..4104)
                     /locus_tag="VFPFJ_10316"
                     /note="active site"
                     /db_xref="CDD:240015"
ORIGIN      

atggactttctcctcggtctattgacgtacctggcttggggacggagtctcgcctttgacgggctccgtatgccacggctcatgatacatgccatattgctggtcagacagatgcgtctggatcaggcagtccctgagcgggggcacatcacggaacccgttgccgcgttgtttgccggcgacagcggcggcggcggcaacaagtttggctacgcgggccaactgacaccggcacaatttctggagcgccagcgtgccgtcctcgggtgttttgtgctcagctccgccgtctcggcctactgcggtcatgttgatgctcttcgttggacgacgcacatggagcaaggccttgcggccatcagcgcaaacaaggcatgtcccacagacgaagcgttcgctctgcaggtccgtttgcagctccttgcctaccaaagcaatcagctccttcgacagcatgacttgaacccaggctctagcgtatcagaggcgtcaacatctcccgctctcatgtctctcgagacacatctggagcagctccacaaacttcagccgggtgtgtcctcaggcctgctgtatgggaagttggccatggcgcacgcacactctgtagagctatgcctcaatctgagcctctactccctcgcgtcgaccgtgccggtcatgctgcgacagtttggcaggatgactcgcagcggacctacgagcggcttgtccgagggcacgacggatagcaggacggcccggcacgagcaaacacagcgcctctggcagtgcacgcgctctggcaaagcgtgcgtgtcggccatgcttggcctctcgccgtccgagcttgcaaacgtctccttcatccagtgggagcagctgacacgctctgtcgccgttcttcatcatctcgccacggaactggaagacccgatttgggaccgacaagcaatgcgtgctgacgctgatgtgcctgggcttttcgaaggtgctgtgaacaagctgcggctagttgccgaatggagaggggagaaggaccccggcgacgcctttaccggcttggcttgcaagttgtgcaagtggtgctccgatggttatggcgtagtagtggagcgcgccactgccgatgcctatcggtcgtttgcaattgccattcaatccttgttgacgggtagtcgccacgccggtcatctttctctagcgccctttgacctcaacggctctggctccggttatcctaatacgaaacgggacctgactactgcatcagataaagttcgtggtacaacctcaggatgtcctaccgcggcggcttcgaccaacgcgggcgcggtggtcctcgtggtggaggaggccctcgcggtggtggaggccatcgcggtggaggcggccctcgcggcggaggagcgcctcgtggtggcttcggccgcggaggtgctgcaggcccgccaacagtgttcagaggacgctatccaggcgagccgacgagccctcagcctcgacagccttcagctcaaggctgcctttcccacacggcccggctacggcaccaagggcaaggccatcgttctctggagcaactacgtcacgtttgccgtcgcccctaagctcgttctgtatcgctacgacatgtccatccagcccagtgctcccaagagaaagacgacgcagattattcgcctcctgcttcagtcgtccgagctcaaccgtgttcgagatgaagtcgttaccgactttcggtcgacactcatatctcgtcaggagtttcccgatcaggccgtgcaaatcacgtatcgcgaggaagacgaggacgatcccgccgagaacgccacggtgtacacggtgacgctccagcgcaccaatgtgctgtccacttctgagctcatcgactacgtcacgtcgacgaacgtgtcggccaattacgacgccaagctgcccatgattcaggctttcaacatctttctgaaccattacgccaagtcgaccggcagccttgccaccatcggttcgtccaagacgtttgccatggacccggccgcctccgaccagtttgacctcggcggttgtctcaccgctatccgcggtttctacgccagtgtccgcgcggccacggcccgcgtgctcgtcaacgtcaatgtcagccatggagcgttctaccaagagggcccgctcgatcggttcatcatgacgtttggcaaggcccgtgggttctacaagctggagcgcatcttgaagaagctccgcgtaacgacaaatcatctccgggagaagagaaaccgcaacggcaaagtcatcccacgggtcaagacaatcattgccttggcaagccctcgtgatggccaaggcctgccgcatccgccgcgcgtgcgagaacacggcgcgggttcaaacggggttgagttctggcttgacgctcagcccgcagcatcgtctgcttcgtccgccggcacgaagaagggaggcaagggcagcaaagcgggcccgcagaaggcaccatccgaaggccactatatctcggtctatgactttttcttgaagacgcacaacatcaagatcgccgacccctccatgcccgtggtcaacgtcggcactcgcgaccggccgtcctaccttcctgcgcaagtctgttccgtgattccaggccaggcagcgcaaacaaagctcgaccctggccaaacgcagaacatgatcaggttcgcagtccggcggccagccgacaatgccacgtcgatcgtcagccagggtctgaggacggctggcctttctcctgccaccaacccgctactggccacgttcggcgtcactgtcggaacggatctcgtcaccgtccatggtcgcattctcgctacgccgagggtcctgtacaaggccaaccaaaaggcccaaatgatggccggcggttggaacatggttccccaaggcggcccggccctcaagttcagcactactatggcgctgaggacttggtcgtgcctgtacattgaattccctccagaattctaccacgacgcgcataagtttacgatggaaggcctcactgatgtgatgaacaaattctacgccgtcttagtaggcactggaatcgccgccagcacccccatgaagccacagcgcgtccgcctccgcgacgaggaggacccggctctagatcagtgtcttcaacgcgctgccaactctttggacctactcttcgtggtactgcctgccggcccgattcctctatacaaccgcatcaagtatctcggcgatgtcaaatatggtatccagacagtttgctgcgtcggcaagaagcttgccaaggctagcggccaagaccaatacttgcgcaacgaggctctcaaggtcaacctcaagctcggcggcagcaaccaagtcgtggagcccatgcgaaacgatttgatcgccgatggtaaaacgatggttgttggcatcgacgtaacccacccttctccgggctccagctctgaggctcccagcatcgccggcatggttgccagtgtggacaagtccatggggcagtggcctgctgccctgagaattcagccccagagacgacaagaaatggtggcggatctcaaagacatgctactgtctcgtctgaggctttggcacaccaagggaaagaatgcctcgcttcccgaaaacatactggtgtatcgcgacggcgtctccgagggccagtaccagctcgtcctgaacgaagagctgccgcttcttcgcgctgcctgtaaagagatttatccaactgacgcacagaaaaagggattgcctcgcatgactgtcgtcattgttggaaagcggcaccacacgcggttctacccgagctcggaggctgacatggatcgttccggcaacaccaagcccggtaccgttgtcgaccgcggcgtcaccgaggggaaaacttgggactttttccttcaggcgcacgctgcactgcagggcactgcgcggccggcacactacgtcgtggtgctggatgagatctttcgtgcgagacacgcgcgttcgagtgccgaggcggctgcctcggatgagctgcaagagttgacgcaaaacatgtcctacacgtttgggcgcgcgacaagggcggtcagcatttgcacgccggcctactatgcggatattctctgcgagagggccagatgctacctgtccagcctgtttgaaggatcgaccaacgcgtctgtgacggacactgctggtggcgttggcagtgcgagtgatgacatcaagacgcacgagaaactcaaggacacgatgttttacatctaa

//