LOCUS       NM_213274               1632 bp    mRNA    linear   VRT 13-AUG-2023
DEFINITION  Danio rerio claudin 5a (cldn5a), mRNA.
ACCESSION   NM_213274
VERSION     NM_213274.1
KEYWORDS    RefSeq.
SOURCE      Danio rerio (zebrafish)
  ORGANISM  Danio rerio
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Ostariophysi;
            Cypriniformes; Danionidae; Danioninae; Danio.
REFERENCE   1  (bases 1 to 1632)
  AUTHORS   Deshwar AR, Cytrynbaum C, Murthy H, Zon J, Chitayat D, Volpatti J,
            Newbury-Ecob R, Ellard S, Allen HL, Yu EP, Noche R, Walker S,
            Scherer SW, Mahida S, Elitt CM, Nicolas G, Goldenberg A,
            Saugier-Veber P, Lecoquierre F, Dabaj I, Meddaugh H, Marble M,
            Keppler-Noreuil KM, Drayson L, Baranano KW, Chassevent A, Agre K,
            Letard P, Bilan F, Le Guyader G, Laquerriere A, Ramsey K, Henderson
            L, Brady L, Tarnopolsky M, Bainbridge M, Friedman J, Capri Y,
            Athayde L, Kok F, Gurgel-Giannetti J, Ramos LLP, Blaser S, Dowling
            JJ and Weksberg R.
  TITLE     Variants in CLDN5 cause a syndrome characterized by seizures,
            microcephaly and brain calcifications
  JOURNAL   Brain 146 (6), 2285-2297 (2023)
   PUBMED   36477332
  REMARK    GeneRIF: Variants in CLDN5 cause a syndrome characterized by
            seizures, microcephaly and brain calcifications.
REFERENCE   2  (bases 1 to 1632)
  AUTHORS   Md Razip NN, Mohd Noor S, Norazit A, Nordin N, Sakeh NM and
            Khaza'ai H.
  TITLE     An Association between Insulin Resistance and Neurodegeneration in
            Zebrafish Larval Model (Danio rerio)
  JOURNAL   Int J Mol Sci 23 (15), 8290 (2022)
   PUBMED   35955446
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1632)
  AUTHORS   Rana AK, Sharma S, Saini SK and Singh D.
  TITLE     Rutin protects hemorrhagic stroke development via supressing
            oxidative stress and inflammatory events in a zebrafish model
  JOURNAL   Eur J Pharmacol 925, 174973 (2022)
   PUBMED   35469838
REFERENCE   4  (bases 1 to 1632)
  AUTHORS   Li Y, Wang C, Zhang L, Chen B, Mo Y and Zhang J.
  TITLE     Claudin-5a is essential for the functional formation of both
            zebrafish blood-brain barrier and blood-cerebrospinal fluid barrier
  JOURNAL   Fluids Barriers CNS 19 (1), 40 (2022)
   PUBMED   35658877
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 1632)
  AUTHORS   Fan RY, Wu JQ, Liu YY, Liu XY, Qian ST, Li CY, Wei P, Song Z and He
            MF.
  TITLE     Zebrafish xenograft model for studying mechanism and treatment of
            non-small cell lung cancer brain metastasis
  JOURNAL   J Exp Clin Cancer Res 40 (1), 371 (2021)
   PUBMED   34801071
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 1632)
  AUTHORS   Baltzegar DA, Reading BJ, Brune ES and Borski RJ.
  TITLE     Phylogenetic revision of the claudin gene family
  JOURNAL   Mar Genomics 11, 17-26 (2013)
   PUBMED   23726886
REFERENCE   7  (bases 1 to 1632)
  AUTHORS   Yin VP, Lepilina A, Smith A and Poss KD.
  TITLE     Regulation of zebrafish heart regeneration by miR-133
  JOURNAL   Dev Biol 365 (2), 319-327 (2012)
   PUBMED   22374218
REFERENCE   8  (bases 1 to 1632)
  AUTHORS   Xie J, Farage E, Sugimoto M and Anand-Apte B.
  TITLE     A novel transgenic zebrafish model for blood-brain and
            blood-retinal barrier development
  JOURNAL   BMC Dev Biol 10, 76 (2010)
   PUBMED   20653957
  REMARK    Publication Status: Online-Only
REFERENCE   9  (bases 1 to 1632)
  AUTHORS   Zhang J, Piontek J, Wolburg H, Piehl C, Liss M, Otten C, Christ A,
            Willnow TE, Blasig IE and Abdelilah-Seyfried S.
  TITLE     Establishment of a neuroepithelial barrier by Claudin5a is
            essential for zebrafish brain ventricular lumen expansion
  JOURNAL   Proc Natl Acad Sci U S A 107 (4), 1425-1430 (2010)
   PUBMED   20080584
  REMARK    GeneRIF: identify Claudin5a as a core component of an early
            cerebral-ventricular barrier system that is required for
            ventricular lumen expansion in the zebrafish embryonic brain before
            the establishment of the embryonic blood-brain barrier.
REFERENCE   10 (bases 1 to 1632)
  AUTHORS   Ebarasi L, He L, Hultenby K, Takemoto M, Betsholtz C, Tryggvason K
            and Majumdar A.
  TITLE     A reverse genetic screen in the zebrafish identifies crb2b as a
            regulator of the glomerular filtration barrier
  JOURNAL   Dev Biol 334 (1), 1-9 (2009)
   PUBMED   19393641
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from BC068370.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC068370.1 [ECO:0000332]
            ##Evidence-Data-END##
FEATURES             Location/Qualifiers
     source          1..1632
                     /organism="Danio rerio"
                     /mol_type="mRNA"
                     /db_xref="taxon:7955"
                     /chromosome="8"
                     /map="8"
     gene            1..1632
                     /gene="cldn5a"
                     /gene_synonym="cldn5; zgc:85723"
                     /note="claudin 5a"
                     /db_xref="GeneID:406559"
                     /db_xref="ZFIN:ZDB-GENE-040426-2442"
     exon            1..1517
                     /gene="cldn5a"
                     /gene_synonym="cldn5; zgc:85723"
                     /inference="alignment:Splign:2.1.0"
     misc_feature    276..278
                     /gene="cldn5a"
                     /gene_synonym="cldn5; zgc:85723"
                     /note="upstream in-frame stop codon"
     CDS             306..953
                     /gene="cldn5a"
                     /gene_synonym="cldn5; zgc:85723"
                     /codon_start=1
                     /product="claudin 5a precursor"
                     /protein_id="NP_998439.1"
                     /db_xref="GeneID:406559"
                     /db_xref="ZFIN:ZDB-GENE-040426-2442"
                     /translation="

MASAALELLGLILCVCGTLLEMVACGLPTWKVTAFIEANIVVAQTIWDGLWMSCAVQSTGQMQCKVHDSMLALSHDLQAARALTVISSVLSVLALMVVIAGAQCTNCIKEDNVKARVVNVGGVIYILSAIFVLVPLCWMANNIISDFYNPQVLPAQKREIGAALYIGWAASALLLLGGSILCCSCPASGSSGYSVKYAPTKRATSNGEYDKRNYV"

     sig_peptide     306..380
                     /gene="cldn5a"
                     /gene_synonym="cldn5; zgc:85723"
                     /inference="COORDINATES: ab initio prediction:SignalP:4.0"
     misc_feature    318..848
                     /gene="cldn5a"
                     /gene_synonym="cldn5; zgc:85723"
                     /note="PMP-22/EMP/MP20/Claudin family; Region:
                     PMP22_Claudin; cl21598"
                     /db_xref="CDD:451326"
ORIGIN      

gacgcagttaggagatgctgtctggctgaccaaagcgcatctaaaccagaggaaaagaaaatacttcggataaaagacgacctgagcaaaattcttccaaaacagaagaaaagaaagcagacaacctgaccaaagctcgtcttaaacagaagacaaaactttggattaaagacgaccaaaccaaagcgcgtcgaaaacagaaaggaaaggacaagaacactttggataaaagacgcaaacttccattagaagaaagcaaacttctaaactccttttagtaccatcaggagtgggaaaagaaagcgatggcctccgcggctttggagctcctgggtctgatcctgtgcgtctgcgggacgcttttggagatggtggcctgcgggctgcccacctggaaggtcaccgccttcatcgaggccaacatcgtggtcgcgcagaccatctgggacggcctgtggatgtcctgcgccgtgcagagcaccggccagatgcaatgcaaagtgcacgactccatgctcgcgctcagccacgacctgcaagccgcgcgcgcgctcacggtcatctcctcggtcttgagcgtgctcgcgctcatggtggtgatcgcaggcgcgcagtgcacaaactgcatcaaagaggacaacgtgaaagcgcgggtggtgaacgtcggaggggtgatctacatcctcagcgccatcttcgtgcttgtgccactgtgctggatggccaataatatcatctccgacttctataacccgcaggtgctgcccgcccagaagcgggagatcggcgcggcgctgtacatcggctgggcggcctctgcgctgctgctgctcgggggaagcatactctgctgctcctgcccggcctctggaagctccgggtactcggtcaaatacgcgcccactaaaagagccacatccaacggggaatacgacaagaggaattacgtgtgaatgcttgagtgtgtgtgtgtgtgtgttgcacatgcagatgaaaactcgatgcttttcgctggactctactttttttttgtttttgtttttaagaggatagttcacccagatgtgaacatttactcactatttactttaacgttacctcaaatggtttttaacctttataaatgtcttttttctgtttgagcacaaaggaagatattttgaagaatgctgtacttaaatgctgtcaccattgacttctatagtacactgtaagagtattttcacaagtgttttccatttatttacggttgtgaattgcattatgagaggttgatctcttgtctgtcgactttagatgttgaaaatttaactctacagtttaccaaagcgactttgattgggattttagtagtttgaaataatgtaatgtatagggaataacatagagagaacagaaaatgtatactggcaggtttttgtagcatactatacaacatcaactcagatgatatatcaatttaaactgttataaaggttaaaaaaagtcactttattaaacttttcaagtttaaaagtaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaa

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