LOCUS       NM_131856               2180 bp    mRNA    linear   VRT 30-SEP-2023
DEFINITION  Danio rerio Kruppel like factor 2a (klf2a), mRNA.
ACCESSION   NM_131856
VERSION     NM_131856.3
KEYWORDS    RefSeq.
SOURCE      Danio rerio (zebrafish)
  ORGANISM  Danio rerio
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Ostariophysi;
            Cypriniformes; Danionidae; Danioninae; Danio.
REFERENCE   1  (bases 1 to 2180)
  AUTHORS   Liu Y, Kossack ME, McFaul ME, Christensen LN, Siebert S, Wyatt SR,
            Kamei CN, Horst S, Arroyo N, Drummond IA, Juliano CE and Draper BW.
  TITLE     Single-cell transcriptome reveals insights into the development and
            function of the zebrafish ovary
  JOURNAL   Elife 11, e76014 (2022)
   PUBMED   35588359
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2180)
  AUTHORS   Tsaryk R, Yucel N, Leonard EV, Diaz N, Bondareva O,
            Odenthal-Schnittler M, Arany Z, Vaquerizas JM, Schnittler H and
            Siekmann AF.
  TITLE     Shear stress switches the association of endothelial enhancers from
            ETV/ETS to KLF transcription factor binding sites
  JOURNAL   Sci Rep 12 (1), 4795 (2022)
   PUBMED   35314737
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2180)
  AUTHORS   Coxam B, Collins RT, Hussmann M, Huisman Y, Meier K, Jung S,
            Bartels-Klein E, Szymborska A, Finotto L, Helker CSM, Stainier DYR,
            Schulte-Merker S and Gerhardt H.
  TITLE     Svep1 stabilises developmental vascular anastomosis in reduced flow
            conditions
  JOURNAL   Development 149 (6) (2022)
   PUBMED   35312765
REFERENCE   4  (bases 1 to 2180)
  AUTHORS   Vignes H, Vagena-Pantoula C, Prakash M, Fukui H, Norden C,
            Mochizuki N, Jug F and Vermot J.
  TITLE     Extracellular mechanical forces drive endocardial cell volume
            decrease during zebrafish cardiac valve morphogenesis
  JOURNAL   Dev Cell 57 (5), 598-609 (2022)
   PUBMED   35245444
REFERENCE   5  (bases 1 to 2180)
  AUTHORS   Chow RW, Fukui H, Chan WX, Tan KSJ, Roth S, Duchemin AL, Messaddeq
            N, Nakajima H, Liu F, Faggianelli-Conrozier N, Klymchenko AS, Choon
            Hwai Y, Mochizuki N and Vermot J.
  TITLE     Cardiac forces regulate zebrafish heart valve delamination by
            modulating Nfat signaling
  JOURNAL   PLoS Biol 20 (1), e3001505 (2022)
   PUBMED   35030171
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2180)
  AUTHORS   Corti P, Young S, Chen CY, Patrick MJ, Rochon ER, Pekkan K and
            Roman BL.
  TITLE     Interaction between alk1 and blood flow in the development of
            arteriovenous malformations
  JOURNAL   Development 138 (8), 1573-1582 (2011)
   PUBMED   21389051
REFERENCE   7  (bases 1 to 2180)
  AUTHORS   Nicoli S, Standley C, Walker P, Hurlstone A, Fogarty KE and Lawson
            ND.
  TITLE     MicroRNA-mediated integration of haemodynamics and Vegf signalling
            during angiogenesis
  JOURNAL   Nature 464 (7292), 1196-1200 (2010)
   PUBMED   20364122
  REMARK    Erratum:[Nature. 2010 Sep 16;467(7313):356]
REFERENCE   8  (bases 1 to 2180)
  AUTHORS   Vermot J, Forouhar AS, Liebling M, Wu D, Plummer D, Gharib M and
            Fraser SE.
  TITLE     Reversing blood flows act through klf2a to ensure normal
            valvulogenesis in the developing heart
  JOURNAL   PLoS Biol 7 (11), e1000246 (2009)
   PUBMED   19924233
  REMARK    GeneRIF: klf2a expression appears to be necessary for normal valve
            formation.
REFERENCE   9  (bases 1 to 2180)
  AUTHORS   Henry TB, Menn FM, Fleming JT, Wilgus J, Compton RN and Sayler GS.
  TITLE     Attributing effects of aqueous C60 nano-aggregates to
            tetrahydrofuran decomposition products in larval zebrafish by
            assessment of gene expression
  JOURNAL   Environ Health Perspect 115 (7), 1059-1065 (2007)
   PUBMED   17637923
REFERENCE   10 (bases 1 to 2180)
  AUTHORS   Yergeau DA, Cornell CN, Parker SK, Zhou Y and Detrich HW 3rd.
  TITLE     bloodthirsty, an RBCC/TRIM gene required for erythropoiesis in
            zebrafish
  JOURNAL   Dev Biol 283 (1), 97-112 (2005)
   PUBMED   15890331
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BX323550.13 and BX908392.13.
            
            On Sep 21, 2011 this sequence version replaced NM_131856.2.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC063933.1, AF392992.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2168446, SAMEA2168447
                                           [ECO:0000348]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-391               BX323550.13        2009-2399           c
            392-1286            BX323550.13        700-1594            c
            1287-1880           BX323550.13        1-594               c
            1881-2180           BX908392.13        177265-177564       c
FEATURES             Location/Qualifiers
     source          1..2180
                     /organism="Danio rerio"
                     /mol_type="mRNA"
                     /strain="Tuebingen"
                     /db_xref="taxon:7955"
                     /chromosome="22"
                     /map="22"
     gene            1..2180
                     /gene="klf2a"
                     /gene_synonym="etID35223.22; fb08e06; wu:fb08e06"
                     /note="Kruppel like factor 2a"
                     /db_xref="GeneID:117508"
                     /db_xref="ZFIN:ZDB-GENE-011109-1"
     exon            1..391
                     /gene="klf2a"
                     /gene_synonym="etID35223.22; fb08e06; wu:fb08e06"
                     /inference="alignment:Splign:2.1.0"
     misc_feature    302..304
                     /gene="klf2a"
                     /gene_synonym="etID35223.22; fb08e06; wu:fb08e06"
                     /note="upstream in-frame stop codon"
     CDS             320..1462
                     /gene="klf2a"
                     /gene_synonym="etID35223.22; fb08e06; wu:fb08e06"
                     /codon_start=1
                     /product="Krueppel-like factor 2a"
                     /protein_id="NP_571931.2"
                     /db_xref="GeneID:117508"
                     /db_xref="ZFIN:ZDB-GENE-011109-1"
                     /translation="

MALSGTILPSISTFSAQKEKCWENRWKDELDRSMHLSCIADLSNMDNLNRITDDEDLDKYLDLEFILANTAGSDNLGLGMAGDYRMQESRNMYNPTTYSVPEINPSPPPPYTTSLMAELLQSDIDTYCQPSLQGRFLVRPAFPTQDISECIKVEPCMDSYGPVRGMVPKVKQEGNGACMRSYEQPRLANSPQGAGSMTPPQSPAELINTDCQSHSQMCHALTFSQSYQGNTGFPHAAPPQMQLPYQSTHHFSMCDDGLAMPNANQRVLLTPPSSPLELDAKPKRGRRTWPRKRTATHTCTFSGCGKTYTKSSHLKAHHRTHTGEKPYHCSWEGCGWKFARSDELTRHFRKHTGHRPFQCHLCERAFSRSDHLALHMKRHM"

     misc_feature    329..1210
                     /gene="klf2a"
                     /gene_synonym="etID35223.22; fb08e06; wu:fb08e06"
                     /note="N-terminal domain of Kruppel-like factor 2; Region:
                     KLF2_N; cd21583"
                     /db_xref="CDD:409229"
     misc_feature    1223..1282
                     /gene="klf2a"
                     /gene_synonym="etID35223.22; fb08e06; wu:fb08e06"
                     /note="C2H2 Zn finger [structural motif]; Region: C2H2 Zn
                     finger"
                     /db_xref="CDD:275368"
     misc_feature    1229..>1375
                     /gene="klf2a"
                     /gene_synonym="etID35223.22; fb08e06; wu:fb08e06"
                     /note="FOG: Zn-finger [General function prediction only];
                     Region: COG5048"
                     /db_xref="CDD:227381"
     misc_feature    order(1235..1237,1241..1243,1247..1249,1253..1258,
                     1265..1270,1277..1279,1325..1327,1331..1333,1343..1348,
                     1355..1360,1367..1369,1409..1411,1415..1417,1421..1423,
                     1427..1432,1439..1444,1451..1453)
                     /gene="klf2a"
                     /gene_synonym="etID35223.22; fb08e06; wu:fb08e06"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]; other site"
                     /db_xref="CDD:275368"
     misc_feature    1304..1372
                     /gene="klf2a"
                     /gene_synonym="etID35223.22; fb08e06; wu:fb08e06"
                     /note="C2H2 Zn finger [structural motif]; Region: C2H2 Zn
                     finger"
                     /db_xref="CDD:275368"
     misc_feature    order(1304..1306,1319..1321,1358..1360,1370..1372)
                     /gene="klf2a"
                     /gene_synonym="etID35223.22; fb08e06; wu:fb08e06"
                     /note="Zn binding site [ion binding]; other site"
                     /db_xref="CDD:275368"
     misc_feature    1346..1423
                     /gene="klf2a"
                     /gene_synonym="etID35223.22; fb08e06; wu:fb08e06"
                     /note="Zinc-finger double domain; Region: zf-H2C2_2;
                     pfam13465"
                     /db_xref="CDD:433230"
     misc_feature    1394..1456
                     /gene="klf2a"
                     /gene_synonym="etID35223.22; fb08e06; wu:fb08e06"
                     /note="C2H2 Zn finger [structural motif]; Region: C2H2 Zn
                     finger"
                     /db_xref="CDD:275368"
     exon            392..1286
                     /gene="klf2a"
                     /gene_synonym="etID35223.22; fb08e06; wu:fb08e06"
                     /inference="alignment:Splign:2.1.0"
     exon            1287..2180
                     /gene="klf2a"
                     /gene_synonym="etID35223.22; fb08e06; wu:fb08e06"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      

gtgatttaaagaagagcccgtgcgctctcaacatccaagacgaaaggcgcacacgccggagagcagcagctatataccggggctaaaattagggacggatagtgcgtatttcctccgcttcttataattggtccagcttgcgctgccgtgatatttataagataaaggtcttccaccactcatactttctgcatgagtgttaaacatagtgtaaagagcagagcagagttgtagtgcactacaaccaaagtttctccgagggattgcaacaaatccccgatctttaaagtttgttttaccttaactgggacgactggaaatggctttgagtggaacgattttaccgtctatttccacattttcggcgcagaaggaaaaatgctgggagaacaggtggaaggatgaactggacaggtccatgcatctcagctgcatagctgacctatctaacatggacaacctcaacagaataacagacgacgaagatcttgacaagtacctggatctggagtttatactggccaacactgcaggttctgacaatctcggactcggcatggcaggcgactacagaatgcaagagtccagaaacatgtacaacccgactacatactcggtgcctgagatcaacccatcaccacctccaccgtacaccaccagcctcatggcagagcttctgcagtctgacattgacacctactgccaaccgtctctccagggaagattcctagtcaggcctgctttccccactcaggacatttcggagtgtatcaaagtggagccctgcatggatagttacggacctgtacgaggaatggttcccaaagtcaaacaagagggcaacggtgcatgcatgagatcctatgagcagccaagactagccaactctccccagggagcaggcagcatgactcccccacagagccctgcggagctcatcaacacagactgtcagtcacactcgcagatgtgccatgccttaacgttcagccagagctatcaaggcaacacaggatttccccacgcagcacctccacagatgcagctgccttaccaaagcacacatcacttcagcatgtgcgacgatgggcttgcaatgcccaacgccaaccaaagagtacttctgacaccaccctcatctcctctagagctggacgccaaaccaaagagggggcgccgcacgtggccgaggaaacgcacggcgactcacacttgcactttttctggatgtggcaaaacttacacaaaaagttctcacttgaaggctcatcacagaacacacacaggcgagaagccctatcattgcagttgggaaggctgtgggtggaagttcgcccgttctgatgaattgacccgtcacttccgcaagcacaccggacaccgacccttccagtgccacctgtgcgaacgtgccttctccaggtccgaccatctcgcccttcatatgaaacgtcatatgtaggaacggactctggaaaatgtggactaaacgcactgaacagtgaacattgtctgtacccccccccccccccccccccccccccccccccctcttatttaaagtatggtgaaggggaaacatgaactgacttgacctattgcttgtagctggttctacacttcaattctttacagaatttacagaaacaaactatatttttcgtttccaaagagcaagttcaactcatactataggctggacctaaacctcaagacggaactggaatcagacagtataatgtgctttaagttaacatgatgtgctgcggacatcacgacagtgccttttaatatgcgtatacatctcacgagtgccacgaatactggcgttttttttaacgacaaatgtatgctattgtgtcaagaaactaaataagctttgtaagacaatgttttcctctttcaaaactgccttaaaagttaacaaaaggggcgttaactgaatgttttaagtaatttgtgcatttatcgacataaacgtttttatttttaaaaatcagggtcttatggtttcgttttttttaaacgcaatttgtatgctgttaacgttgaattgcagactgtcccatgtttggatgacatgataagggctatgtgagttgttctggaccaaagcattttatgtacataatttatacactgtatatattgtacatattaaacttaaattgcgatataaa

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