LOCUS       NM_131040               1823 bp    mRNA    linear   VRT 21-AUG-2023
DEFINITION  Danio rerio engrailed homeobox 2b (en2b), mRNA.
ACCESSION   NM_131040
VERSION     NM_131040.1
KEYWORDS    RefSeq.
SOURCE      Danio rerio (zebrafish)
  ORGANISM  Danio rerio
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Ostariophysi;
            Cypriniformes; Danionidae; Danioninae; Danio.
REFERENCE   1  (bases 1 to 1823)
  AUTHORS   Branigan GL, Olsen KS, Burda I, Haemmerle MW, Ho J, Venuto A,
            D'Antonio ND, Briggs IE and DiBenedetto AJ.
  TITLE     Zebrafish Paralogs brd2a and brd2b Are Needed for Proper
            Circulatory, Excretory and Central Nervous System Formation and Act
            as Genetic Antagonists during Development
  JOURNAL   J Dev Biol 9 (4), 46 (2021)
   PUBMED   34842711
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1823)
  AUTHORS   Amblard I, Thauvin M, Rampon C, Queguiner I, Pak VV, Belousov V,
            Prochiantz A, Volovitch M, Joliot A and Vriz S.
  TITLE     H2O2 and Engrailed 2 paracrine activity synergize to shape the
            zebrafish optic tectum
  JOURNAL   Commun Biol 3 (1), 536 (2020)
   PUBMED   32994473
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1823)
  AUTHORS   Liu Y, Zhu Z, Ho IHT, Shi Y, Li J, Wang X, Chan MTV and Cheng CHK.
  TITLE     Genetic Deletion of miR-430 Disrupts Maternal-Zygotic Transition
            and Embryonic Body Plan
  JOURNAL   Front Genet 11, 853 (2020)
   PUBMED   32849832
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1823)
  AUTHORS   Farnsworth DR, Saunders LM and Miller AC.
  TITLE     A single-cell transcriptome atlas for zebrafish development
  JOURNAL   Dev Biol 459 (2), 100-108 (2020)
   PUBMED   31782996
REFERENCE   5  (bases 1 to 1823)
  AUTHORS   Farrell JA, Wang Y, Riesenfeld SJ, Shekhar K, Regev A and Schier
            AF.
  TITLE     Single-cell reconstruction of developmental trajectories during
            zebrafish embryogenesis
  JOURNAL   Science 360 (6392) (2018)
   PUBMED   29700225
REFERENCE   6  (bases 1 to 1823)
  AUTHORS   Paragas VB, Zhang YZ, Haugland RP and Singer VL.
  TITLE     The ELF-97 alkaline phosphatase substrate provides a bright,
            photostable, fluorescent signal amplification method for FISH
  JOURNAL   J Histochem Cytochem 45 (3), 345-357 (1997)
   PUBMED   9071316
REFERENCE   7  (bases 1 to 1823)
  AUTHORS   Moens CB, Yan YL, Appel B, Force AG and Kimmel CB.
  TITLE     valentino: a zebrafish gene required for normal hindbrain
            segmentation
  JOURNAL   Development 122 (12), 3981-3990 (1996)
   PUBMED   9012518
REFERENCE   8  (bases 1 to 1823)
  AUTHORS   Ekker M, Speevak MD, Martin CC, Joly L, Giroux G and Chevrette M.
  TITLE     Stable transfer of zebrafish chromosome segments into mouse cells
  JOURNAL   Genomics 33 (1), 57-64 (1996)
   PUBMED   8617510
REFERENCE   9  (bases 1 to 1823)
  AUTHORS   Hatta K, Bremiller R, Westerfield M and Kimmel CB.
  TITLE     Diversity of expression of engrailed-like antigens in zebrafish
  JOURNAL   Development 112 (3), 821-832 (1991)
   PUBMED   1682127
REFERENCE   10 (bases 1 to 1823)
  AUTHORS   Holland PW and Williams NA.
  TITLE     Conservation of engrailed-like homeobox sequences during vertebrate
            evolution
  JOURNAL   FEBS Lett 277 (1-2), 250-252 (1990)
   PUBMED   1980115
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from X68447.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: X68447.1, BC071290.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2168447, SAMEA3505370
                                           [ECO:0000348]
            ##Evidence-Data-END##
FEATURES             Location/Qualifiers
     source          1..1823
                     /organism="Danio rerio"
                     /mol_type="mRNA"
                     /db_xref="taxon:7955"
                     /chromosome="2"
                     /map="2"
     gene            1..1823
                     /gene="en2b"
                     /gene_synonym="en3; ENG-3; eng2b; eng3; ZF-EN-1"
                     /note="engrailed homeobox 2b"
                     /db_xref="GeneID:30238"
                     /db_xref="ZFIN:ZDB-GENE-980526-40"
     exon            1..806
                     /gene="en2b"
                     /gene_synonym="en3; ENG-3; eng2b; eng3; ZF-EN-1"
                     /inference="alignment:Splign:2.1.0"
     misc_feature    311..313
                     /gene="en2b"
                     /gene_synonym="en3; ENG-3; eng2b; eng3; ZF-EN-1"
                     /note="upstream in-frame stop codon"
     CDS             338..1123
                     /gene="en2b"
                     /gene_synonym="en3; ENG-3; eng2b; eng3; ZF-EN-1"
                     /note="homeobox protein en-2b; engrailed 3; engrailed 2b"
                     /codon_start=1
                     /product="homeobox protein engrailed-2b"
                     /protein_id="NP_571115.1"
                     /db_xref="GeneID:30238"
                     /db_xref="ZFIN:ZDB-GENE-980526-40"
                     /translation="

MEENDHSNRDVERQDSGDESNRAILPLLQAPGNVLPHRITNFYIDNILRPDFGRRKEGSRRDEINIVERENRCPSAPGSGQVAPVSGEGTSSPRAVNASKKTDISTDESLKSRAETGDQCLSSDSDCSQRCAAQAKQPMLWPAWVYCTRYSDRPSSGPRSRKPKKKTPTKEDKRPRTAFTAEQLQRLKNEFQNNRYLTEQRRQALAQELGLNESQIKIWFQNKRAKIKKATGNKNTLAVHLMAQGLYNHATVTKDDKSDSD"

     misc_feature    338..409
                     /gene="en2b"
                     /gene_synonym="en3; ENG-3; eng2b; eng3; ZF-EN-1"
                     /note="propagated from UniProtKB/Swiss-Prot (P31533.2);
                     Region: Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite"
     misc_feature    494..712
                     /gene="en2b"
                     /gene_synonym="en3; ENG-3; eng2b; eng3; ZF-EN-1"
                     /note="propagated from UniProtKB/Swiss-Prot (P31533.2);
                     Region: Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite"
     misc_feature    791..865
                     /gene="en2b"
                     /gene_synonym="en3; ENG-3; eng2b; eng3; ZF-EN-1"
                     /note="propagated from UniProtKB/Swiss-Prot (P31533.2);
                     Region: Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite"
     misc_feature    order(854..868,872..874,923..925,941..943,980..982,
                     986..991,998..1003,1007..1015,1019..1024)
                     /gene="en2b"
                     /gene_synonym="en3; ENG-3; eng2b; eng3; ZF-EN-1"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:238039"
     misc_feature    860..1021
                     /gene="en2b"
                     /gene_synonym="en3; ENG-3; eng2b; eng3; ZF-EN-1"
                     /note="Homeobox domain; Region: Homeobox; pfam00046"
                     /db_xref="CDD:425441"
     misc_feature    order(860..862,869..871,989..991,998..1003,1010..1012)
                     /gene="en2b"
                     /gene_synonym="en3; ENG-3; eng2b; eng3; ZF-EN-1"
                     /note="specific DNA base contacts [nucleotide binding];
                     other site"
                     /db_xref="CDD:238039"
     misc_feature    1025..1117
                     /gene="en2b"
                     /gene_synonym="en3; ENG-3; eng2b; eng3; ZF-EN-1"
                     /note="Engrailed homeobox C-terminal signature domain;
                     Region: Engrail_1_C_sig; pfam10525"
                     /db_xref="CDD:431338"
     exon            807..1819
                     /gene="en2b"
                     /gene_synonym="en3; ENG-3; eng2b; eng3; ZF-EN-1"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      

gcgtttccttatttacttgcgcaggtctactgctttgattctgaatagattacatttccctgtatgttccaaagtagatataaagacaattacaagaggcacaatgtggaattaatttataaaactatgtttttatttttacgcgcacaagacggtttcgtcgcatttacccgacaaaaaaactgaaaaaaggaatttgttctttttcccgttcgtttctttttgcagagaaactcagaaataaggaaaatatactactaaaactggaacacaaacatacttgtgtgcaagagagcgaaaggggtggggttaaaaagaacatttggagagtttcactatggaagaaaatgatcatagcaacagagatgtggagcgccaggattcgggcgacgagtccaatagggctattcttcccctgttgcaggctcctggaaacgtactccctcacagaatcaccaacttttacatcgacaatattttaagaccagactttggtcgcaggaaagaaggaagcagacgcgacgaaattaacatagttgagagagagaaccgctgtccatcggctcccggatcagggcaggtagctccagtatcgggggaaggaacctcgagccctcgcgctgtaaacgcatctaaaaaaactgatattagcacggacgaatctctgaaatcccgtgcagagactggagatcagtgtttaagttccgattcggactgctcacaaagatgcgctgcgcaggcgaaacagccaatgctttggccggcttgggtatattgcacaagatattcagacagaccttcatcaggaccaagatcacgcaaaccaaagaagaaaaccccaaccaaggaagacaagcgtccgagaacagcattcacagcggagcaactacagagactcaagaatgaattccagaataatcgttacctgacggagcaaaggagacaagcgttggcccaggaactcggcctgaacgagtctcaaatcaaaatctggtttcaaaacaaaagggcaaagatcaaaaaagcaacggggaacaaaaacacacttgccgtgcacctgatggcacagggactttacaatcacgccacagtaacaaaggacgacaaatcagacagtgattaacacggaggacaggatatcttaaatgcaataattctaaacaagggccagtgtacaaaataccagcattaaacggatgaaaactatgtatgagatgtgattttgcaatattatatgtatatatactttgtgtagggtggtgtacaacttttttttaattagcatcactgctgtgcactgactgaacttcagatttattgcgcgaggaaaaggacgcttctttatttaaaaaaaaaaaaagaaaaaaaaaggagcgatttgatacacaccttgacgttgagccacacgtgaacgtgtttaatccatgctgaagtccaaagatttttcctgctgcattcaggcgactgtgatttaaaataaatggtaaaaatgttttatctgatagatgtttgtttgtttgcataattttattactggatgaatgttttacttaattgacttgagttacagagacatggtatttaaaaagacaacattatcaacatattccacattaaagaattcagtttatttcatatttatggtctattgtctggcaaggatggtttacctcacatctcaacattttcaggaaaaagatcaacatctgaatgcatacgagtcagtaaaactcaaaaccacactaatgcaagtacttgactttgttgtctgttgaatttagctgggagtgtacaataaaagttatcaactttttcaaaaaaaa

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