LOCUS       NM_001362619            7214 bp    mRNA    linear   VRT 16-OCT-2021
DEFINITION  Danio rerio G protein-coupled receptor kinase 4 (grk4), transcript
            variant 1, mRNA.
ACCESSION   NM_001362619 XM_009306441
VERSION     NM_001362619.1
KEYWORDS    RefSeq.
SOURCE      Danio rerio (zebrafish)
  ORGANISM  Danio rerio
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Ostariophysi;
            Cypriniformes; Danionidae; Danioninae; Danio.
REFERENCE   1  (bases 1 to 7214)
  AUTHORS   Guo H, Du X, Zhang Y, Wu J, Wang C, Li M, Hua X, Zhang XA and Yan
            J.
  TITLE     Specific miRNA-G Protein-Coupled Receptor Networks Regulate
            Sox9a/Sox9b Activities to Promote Gonadal Rejuvenation in Zebrafish
  JOURNAL   Stem Cells 37 (9), 1189-1199 (2019)
   PUBMED   31116894
REFERENCE   2  (bases 1 to 7214)
  AUTHORS   Bayes A, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A,
            Choudhary JS, Emes RD and Grant SG.
  TITLE     Evolution of complexity in the zebrafish synapse proteome
  JOURNAL   Nat Commun 8, 14613 (2017)
   PUBMED   28252024
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 7214)
  AUTHORS   Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I,
            Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y and
            Bobe J.
  TITLE     Gene evolution and gene expression after whole genome duplication
            in fish: the PhyloFish database
  JOURNAL   BMC Genomics 17, 368 (2016)
   PUBMED   27189481
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 7214)
  AUTHORS   Zhao Z, Lee RT, Pusapati GV, Iyu A, Rohatgi R and Ingham PW.
  TITLE     An essential role for Grk2 in Hedgehog signalling downstream of
            Smoothened
  JOURNAL   EMBO Rep 17 (5), 739-752 (2016)
   PUBMED   27113758
REFERENCE   5  (bases 1 to 7214)
  AUTHORS   Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M,
            Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD,
            Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov
            GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W and
            Hertzano R.
  TITLE     RFX transcription factors are essential for hearing in mice
  JOURNAL   Nat Commun 6, 8549 (2015)
   PUBMED   26469318
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 7214)
  AUTHORS   Valanne S, Myllymaki H, Kallio J, Schmid MR, Kleino A, Murumagi A,
            Airaksinen L, Kotipelto T, Kaustio M, Ulvila J, Esfahani SS,
            Engstrom Y, Silvennoinen O, Hultmark D, Parikka M and Ramet M.
  TITLE     Genome-wide RNA interference in Drosophila cells identifies G
            protein-coupled receptor kinase 2 as a conserved regulator of
            NF-kappaB signaling
  JOURNAL   J Immunol 184 (11), 6188-6198 (2010)
   PUBMED   20421637
REFERENCE   7  (bases 1 to 7214)
  AUTHORS   Miranda CL, Collodi P, Zhao X, Barnes DW and Buhler DR.
  TITLE     Regulation of cytochrome P450 expression in a novel liver cell line
            from zebrafish (Brachydanio rerio)
  JOURNAL   Arch Biochem Biophys 305 (2), 320-327 (1993)
   PUBMED   8373170
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BX908747.25.
            
            On Apr 21, 2018 this sequence version replaced XM_009306441.2.
            
            Transcript Variant: This variant (1) encodes the longer isoform
            (1).
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            CDS exon combination :: GFIL01011992.1 [ECO:0000331]
            RNAseq introns       :: mixed/partial sample support SAMEA2168446,
                                    SAMEA2168447 [ECO:0000350]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-358               BX908747.25        139048-139405
            359-454             BX908747.25        157223-157318
            455-567             BX908747.25        164550-164662
            568-645             BX908747.25        165128-165205
            646-746             BX908747.25        165360-165460
            747-839             BX908747.25        171634-171726
            840-903             BX908747.25        176701-176764
            904-1044            BX908747.25        177588-177728
            1045-1235           BX908747.25        187067-187257
            1236-1273           BX908747.25        190542-190579
            1274-1363           BX908747.25        194116-194205
            1364-1572           BX908747.25        194291-194499
            1573-1710           BX908747.25        199203-199340
            1711-1848           BX908747.25        199445-199582
            1849-1980           BX908747.25        200549-200680
            1981-7214           BX908747.25        204613-209846
FEATURES             Location/Qualifiers
     source          1..7214
                     /organism="Danio rerio"
                     /mol_type="mRNA"
                     /strain="Tuebingen"
                     /db_xref="taxon:7955"
                     /chromosome="1"
                     /map="1"
     gene            1..7214
                     /gene="grk4"
                     /gene_synonym="zgc:153020"
                     /note="G protein-coupled receptor kinase 4"
                     /db_xref="GeneID:560761"
                     /db_xref="ZFIN:ZDB-GENE-060929-1198"
     exon            1..358
                     /gene="grk4"
                     /gene_synonym="zgc:153020"
                     /inference="alignment:Splign:2.1.0"
     misc_feature    268..270
                     /gene="grk4"
                     /gene_synonym="zgc:153020"
                     /note="upstream in-frame stop codon"
     CDS             307..2046
                     /gene="grk4"
                     /gene_synonym="zgc:153020"
                     /EC_number="2.7.1.-"
                     /note="isoform 1 is encoded by transcript variant 1; GRK5"
                     /codon_start=1
                     /product="G protein-coupled receptor kinase 4 isoform 1"
                     /protein_id="NP_001349548.1"
                     /db_xref="GeneID:560761"
                     /db_xref="ZFIN:ZDB-GENE-060929-1198"
                     /translation="

MEIENIVANTVLLKAREGGGGKRNGRSKKWKEMLKLPHISQCEELRRTIERDYTSLCEKQPIGRQLFRQFCDTQPKLRRCIEFLDSVAMYQVAPDEKRRDAGLNILDTYFNNGSAAHLPEMAQDVVGECRQKLEQSPCKELFQECTRIVREYLSGAPFSAYQETMYFSRFLQWKWLERQPVTKNTFRHYRVLGKGGFGEVCACQVRATGKMYACKKLEKKRVKKRKGEAMALNEKRILEKVNSSFVVSLAYAYETKDALCLVLTIMNGGDLKFHIYNMGNSGFDEQRAIFYAAEICCGLEDLHRERIVYRDLKPENILLDDRGHIRISDLGLAVQIPEGETIRGRVGTVGYMAPEVIQNESYTFSPDWWGLGCLIYEMIQGQSPFRRRKERVKREEVDRRVCEDQEEYTEKFSEDAKDICRQLLCKDPKERLGCRGSGAAEVKLHPIFRNINFKRLEANMLDPPFCPDPRAVYCKDVLDIEQFSTVKGVNLDPTDDDFYHKFVTGSVSIPWQNEMIEMECFKEINVFEMDGALCADLDVNRPNPAPKRGFFYRLFHREVCFGSANSEEEEKEEKEEEPS"

     misc_feature    460..855
                     /gene="grk4"
                     /gene_synonym="zgc:153020"
                     /note="Regulator of G protein signaling domain (RGS) found
                     in G protein-coupled receptor kinase 4 (GRK4); Region:
                     RGS_GRK4; cd08750"
                     /db_xref="CDD:188704"
     misc_feature    order(511..513,520..522,793..795,799..804,808..813)
                     /gene="grk4"
                     /gene_synonym="zgc:153020"
                     /note="putative dimer interface [polypeptide binding];
                     other site"
                     /db_xref="CDD:188704"
     misc_feature    859..1713
                     /gene="grk4"
                     /gene_synonym="zgc:153020"
                     /note="Protein Kinases, catalytic domain; Region:
                     PKc_like; cl21453"
                     /db_xref="CDD:451246"
     misc_feature    order(880..891,898..900,904..906,943..945,949..951,
                     1045..1047,1093..1104,1237..1239,1249..1254,1258..1260,
                     1288..1293)
                     /gene="grk4"
                     /gene_synonym="zgc:153020"
                     /note="ATP binding site [chemical binding]; other site"
                     /db_xref="CDD:270870"
     exon            359..454
                     /gene="grk4"
                     /gene_synonym="zgc:153020"
                     /inference="alignment:Splign:2.1.0"
     exon            455..567
                     /gene="grk4"
                     /gene_synonym="zgc:153020"
                     /inference="alignment:Splign:2.1.0"
     exon            568..645
                     /gene="grk4"
                     /gene_synonym="zgc:153020"
                     /inference="alignment:Splign:2.1.0"
     exon            646..746
                     /gene="grk4"
                     /gene_synonym="zgc:153020"
                     /inference="alignment:Splign:2.1.0"
     exon            747..839
                     /gene="grk4"
                     /gene_synonym="zgc:153020"
                     /inference="alignment:Splign:2.1.0"
     exon            840..903
                     /gene="grk4"
                     /gene_synonym="zgc:153020"
                     /inference="alignment:Splign:2.1.0"
     exon            904..1044
                     /gene="grk4"
                     /gene_synonym="zgc:153020"
                     /inference="alignment:Splign:2.1.0"
     exon            1045..1235
                     /gene="grk4"
                     /gene_synonym="zgc:153020"
                     /inference="alignment:Splign:2.1.0"
     exon            1236..1273
                     /gene="grk4"
                     /gene_synonym="zgc:153020"
                     /inference="alignment:Splign:2.1.0"
     exon            1274..1363
                     /gene="grk4"
                     /gene_synonym="zgc:153020"
                     /inference="alignment:Splign:2.1.0"
     exon            1364..1572
                     /gene="grk4"
                     /gene_synonym="zgc:153020"
                     /inference="alignment:Splign:2.1.0"
     exon            1573..1710
                     /gene="grk4"
                     /gene_synonym="zgc:153020"
                     /inference="alignment:Splign:2.1.0"
     exon            1711..1848
                     /gene="grk4"
                     /gene_synonym="zgc:153020"
                     /inference="alignment:Splign:2.1.0"
     exon            1849..1980
                     /gene="grk4"
                     /gene_synonym="zgc:153020"
                     /inference="alignment:Splign:2.1.0"
     exon            1981..7214
                     /gene="grk4"
                     /gene_synonym="zgc:153020"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      

gtaagcgagagctcgagacaggaaggactgctgagcactgaatgctaacaagggggagagagagtacatgtccacggcgagcggaaaggtggggggttcaacagcgagatatggagaaatacaattaacctgcaatgtgccaacgccaaactcacgccgatcataaccatcctcaccaccgatcatcatcatcatcatcatcctcaccctcatcaccaccaccagcagcaacggcgcgaccgacctcgtcttttttctcctaagaagtgaaatgtatcgggactgcatgggatctttcttctgacaatggagattgagaatatcgtggccaacaccgtgctgctgaaggcgagggaaggaggcggagggaagcgtaatggacggagcaagaaatggaaggagatgctgaaattgcctcatatcagtcagtgtgaggaactgagacgcacaattgagagggattatacaagcctttgtgaaaagcagccaattggacgtcagttgtttcgtcaattctgtgacactcagcccaaattacggcgctgtatagagttcctggattctgtggcaatgtatcaggtggctccggatgagaagaggagagacgccggtttaaatattttagacacatattttaataacgggtcagccgctcatctaccagagatggctcaggatgtggtcggggaatgcaggcagaaactggaacagagtccatgtaaagagctgttccaggagtgtaccaggatagttcgtgaatatttaagtggagcgccattctcggcttatcaggagactatgtacttttcccgttttcttcagtggaagtggttggagaggcaaccagtaaccaaaaacacattcagacattacagggtattgggcaagggcggatttggtgaggtttgtgcctgccaggttcgtgccactggtaagatgtacgcctgtaaaaagctggagaagaaacgtgtgaagaaaagaaaaggtgaagcaatggcattaaatgaaaaacgcattttagaaaaagttaacagtagttttgtagttagtttggcatatgcgtatgagactaaggacgctctatgtttggtgttgaccataatgaacggtggtgatctgaagtttcatatctataacatgggaaactcaggtttcgatgagcagcgggccatcttttacgcggcagagatctgttgcggattagaggatctgcatcgagagcggatcgtctacagagacttgaaaccagaaaatattctgctagatgatcgcgggcacatccgtatttctgatctgggactggccgtgcaaattccagagggcgagacgatacgagggcgtgttggcacagtggggtacatggcaccagaggtcatccagaacgagagctacaccttcagtcctgactggtggggtttgggctgtctaatatacgaaatgattcagggtcagtctcctttccgtcgccgtaaagagcgagtgaagagagaggaagtggaccggcgagtttgtgaggaccaggaggaatatactgagaagttctcagaagacgctaaagacatctgccgtcagttgttatgtaaagatccaaaggagcgcttgggctgtcggggcagtggagcggccgaagtcaaactgcatcccattttccgcaacatcaacttcaagcggctggaggccaacatgctggacccgccgttctgtccagacccgcgagcagtttactgtaaggacgtcctggacatagagcagttttcgacagtcaaaggtgtaaacctggacccaacagatgacgatttctaccacaagtttgtaacagggagtgtctccatcccatggcaaaatgagatgattgagatggagtgttttaaggagatcaacgtgttcgagatggacggagcgctgtgtgctgatctggatgtgaacagacccaatccagcaccgaagcgaggattcttctaccgtttatttcaccgagaggtttgttttgggagtgccaacagtgaggaggaggagaaagaggagaaggaggaggagccatcttgacttaaaaccactcccacttcccatcagctgccaatcagaatgctaaacgtatgaatgttcaactgtatttatgaactgtatcaaactgtatcataattaaagaaaagggtatgagtttaaagaattttgtacatttgtacttgaattatgtgtagatgtatattttcactaaaggttgtattgtacaaaaagccatagaaataccacttgaatgcatatataatgtttttattgttattatttttcatctgtactgaatatgaaatgtgtatttctttttaagcacaatataaacactcaccttttgtttccaatgctaacgctctgttagcactgcctttgtttgtatgttaacttagatcgtttggctggacagatccaccgcgcttcttttgtacagcagggtaagatcaatcacacataaagagggagcagtacaaatgatctattcatatcatatcatttacagtggtcaaccagaatgctaatatcaaaagcaggggtggctgtggtcaaataaaaacaaaaaaacaaactgtatatagcctagatatataaaaacgaggttcaaaagtctgagaataccaagtaaaatgctttagaatatatattcagaaaaatctggactgaaaatgaattgcgtgcaataataacacctttgttaagcaatatgtggctaattttgagattcgaactgttaggaaatatactcataatttcaaaattagcctaatgttgatgtattaaaatatattaatgctttattgccaccttcactcattccccccccccccccccccccccccccaatttttgtcaaatttgacatgcctttttcttcaacccaggctcattctgaaaatgtacccccgtatacatttctggagagcactaaatacatccctggagctacgtcattgcttttttttttttcgaattccccagaggccgctgtgtatgctttttaaaatctcaattttttctagcaagtaccatttaaacctgctgttcttgcgtaaatccaccagaggctgctgtaaactgtccttccctaaacccaaccaatagtgtttccaatagcaccgattgacccgccttcgctaaccccaaccgacagtttaaaaagcaatccaaaaaaagaaaagcccttgcctgatttttaccatgttttcagatttttccacattctcaccctgttatttacttcattttattttttggcttctgtttttgtcttacccgctttctggaaccattcgaaccctgtcgttggggtcaactcctctctgcgtctcaagtcatacatgactcgacatacatggcgagctgctggacaaactgataacagtagaaacgctgtttacatggaagtcagcggtcagctggaaagcgagaaaagaccaccctgtagccttcattttaaagacgaaatgctgccatatgtacctctggctacataattcatgatcttcttaaatgtatatagggctatgtttttagagtgagcctatgttgttgttgttgttttgtaaatgtttatttttagggctaaattgaatatataactccagattttcagtgtgtcctcagacttctgaaccccacctgtgtgttgacctcattgacaagctgtctggattttgtacaaaaggaaagtgttcatacatatgttaatgataatgggtgtgccaatagctagtgccaatagcctcaaaacaaccagatgattaataggtccaatccaatgactaataacaatgtgtaacttgcgggttgtggaagtgtaacgtcattcattttctccatagggaatttttttttttaaactgataactgcagtcttgttgtgagctctgaggttcttgattgtatatgctgttgctgaacttgtctattcacattaaatcactttattatttcaacagtcatttttgattgtagaatttggccataacaacgacattacccatgatgctgtgcagaaaaccaaccaatcagacagttgcagcgtttctgattgtagaatctggcaaaagcaaccaatttacccatgatactgtgcagaaaactcaccaatcagactgttgcagtgtttttgatcatagaattgggccataacaaccacattaaacattatgctgtgcagaaaacccaccaatcagagagttgtagcattttacattttagaatttggccataacaacaacattacccatgatgctatgcagaaaacccaccaatcagacagttgcagctgacaaagcaactttacattatatcactttattatagtcaattttgatcgtagaattgggccataacaacaacattacccgtgatgctgtgcagaaatgccaccaatcagacagttgcagcttttttgattgtagaattggtccataacaactacattacccatgatgctgtgcattaaagccaccaatcagacagttgcagcgattttaattgtagaatttcgccataactacaatacccatgatgctgtgcagaaaacctatcaatcagaccgttgcagcgtttttgattgtagaatttggccaaaacaactacattacccatgatgctgtgcagaaaagttaccaatcggacagttgctgctaacaaagcaacttcagattaaatcaccttttttattttaacgatcatttttgattgtagaatctggccataacaactacattacccaacatgctgtgtgcataaaacccaccaatcagacagttgcagcattttttattatagaatttggctataacaactacattacccatgatgctgtgcacaaaacccaccaatcaggcacttacagcatttttgatagtagaatttggccataacaactacattacccatgatgctgtgcagaaaatccatcaatcagacagttgctgcatttttgattgtagaatttggccataacagccacattacccatgatgctttgcagaaaacccaccaatcagacagttgtagcgttttaatggtagaattgggacacaacaaccacattacccatgatgctgtgcagaaaacccaccaattagacagttgcagctaacatagcacgtcaagagatctttaactccaaaaacttccatgaagcaccccaaaatattttccaaaaaagcttaaaatatggataaaacaaacacctttaattgaacaattttttatttcttaatcgttttaatctgattatgttttgtgcaatgctttatgggattgtagttctttttctcatttaacctgttcagtatgtcttgtacttcttctcagatttgtaaggttgtgattcacctcagatgattagacagttttattactcaaaaaaataaatattttttaatccctatggtaaaatgaacaggcaaatactttctgaaaaagtgaacgtcagcactagtcgtaaaattgttctttcattaaattcatgcagctttaacgaaatgcatgaaaccaataatctctgtgcatcgggtgactaatcccagttttggttacgcattaacaagacacatatgtatgttaattaacgtagtcaccgtagctttcagatgcatcaacgacagggaaccaggcatgttgttcaacgatagactaatatgagttttttttttcagtggcagataatcgatatctagtacctagtaatatggacatgtacgtaaataagtgactgaatgatggcatattacactatatttactcaaaacccacatacgattgaacatataaaatgtttactatccattaacatggctgttgttcggtttttggagttgatacaaggaaatgttaattactctagctatgtgtcagtagttttcaggccgctaatctgaaatgatttattttttccgttttgttgactgtttgctaatatccactctcatggttacatttagtgccatcaggaatctttaaaccaacgaaataagcttcatctttgaatttgctcttgaacgagctgtcgattttgccttaggtgtcatgtgttcggtcgacattccaaacggagtatcgatcgatcccttaacgataatcatatgatgtaattaatgactgtgcagtttgttcgttatacacttgcacattttacattttgctttgtatgtatttgtgtggcatattttaatcgcaatgaaggcttccatttagtgatgggaattagcatctcgatcttacgagaaactattttacatgtcatcagaaaagtggctaacttgtacaaattcgtacggtttcatttgcatgaaaacataagatttttttttaagtaggagtggccccaaaccccacccaaaacttattcctcattgggtggaattagtgaatcttattaaaatgtacatatgagatcgtacaaattaatatgaattagccactacatcaaaaagttacgaattgagaattgcgttggaattagtctcaaatgtattgttcataaattgggtaatattacatttgatttaaatggaggaagtgaaaattagttttgcttccattcagtggtgagaattagcagctcgatctcacgaggaactattttacatgtcagaaaagaggctaattcgtataaatgcatacaatttaatttgtatgaaaacgtacaattttaaaagagtaggtgtggcctcaaaccccacccctaaccctacccgtcattgggtggaattatcgaatcttattaaaatttacgaattagatcgtacaattaatacaaaatagccactacatcaaaaagttacgaatttagaattgcgttggaattcaaatgtcaaatgtatctttcggaaattgcataatattacatttgatttaaatggaggaagtggaaattagttttgatctaaaaaatgcttttgtctaacgcaccttcgaaatcagagtcccaaccaatggcgtgagtcgggggcggggctataacatgcttcagcactgacagtaaagctcaaataggaaccattattttacctccatcacccatttttctctttatgaggatctttatggtcccgtttgcaaagctgtgaactgaaaacaactcctcttctgttattcccatatgtgtgcatgttctactgtgtccatggttttacaagaggatgagcactcactttagttgacaataaagttatgagttgaca

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