LOCUS       NM_001317223            2986 bp    mRNA    linear   VRT 16-OCT-2021
DEFINITION  Danio rerio argonaute RISC component 1 (ago1), mRNA.
ACCESSION   NM_001317223 XM_694292
VERSION     NM_001317223.1
KEYWORDS    RefSeq.
SOURCE      Danio rerio (zebrafish)
  ORGANISM  Danio rerio
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Ostariophysi;
            Cypriniformes; Danionidae; Danioninae; Danio.
REFERENCE   1  (bases 1 to 2986)
  AUTHORS   Raman R, Ryon M and Jagadeeswaran P.
  TITLE     RNaseH-mediated simultaneous piggyback knockdown of multiple genes
            in adult zebrafish
  JOURNAL   Sci Rep 10 (1), 20187 (2020)
   PUBMED   33214638
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2986)
  AUTHORS   Liu Y, Zhu Z, Ho IHT, Shi Y, Li J, Wang X, Chan MTV and Cheng CHK.
  TITLE     Genetic Deletion of miR-430 Disrupts Maternal-Zygotic Transition
            and Embryonic Body Plan
  JOURNAL   Front Genet 11, 853 (2020)
   PUBMED   32849832
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2986)
  AUTHORS   Bayes A, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A,
            Choudhary JS, Emes RD and Grant SG.
  TITLE     Evolution of complexity in the zebrafish synapse proteome
  JOURNAL   Nat Commun 8, 14613 (2017)
   PUBMED   28252024
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2986)
  AUTHORS   Katz S, Cussigh D, Urban N, Blomfield I, Guillemot F, Bally-Cuif L
            and Coolen M.
  TITLE     A Nuclear Role for miR-9 and Argonaute Proteins in Balancing
            Quiescent and Activated Neural Stem Cell States
  JOURNAL   Cell Rep 17 (5), 1383-1398 (2016)
   PUBMED   27783951
REFERENCE   5  (bases 1 to 2986)
  AUTHORS   Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M,
            Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD,
            Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov
            GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W and
            Hertzano R.
  TITLE     RFX transcription factors are essential for hearing in mice
  JOURNAL   Nat Commun 6, 8549 (2015)
   PUBMED   26469318
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2986)
  AUTHORS   McFarlane L, Svingen T, Braasch I, Koopman P, Schartl M and Wilhelm
            D.
  TITLE     Expansion of the Ago gene family in the teleost clade
  JOURNAL   Dev Genes Evol 221 (2), 95-104 (2011)
   PUBMED   21556854
REFERENCE   7  (bases 1 to 2986)
  AUTHORS   Sander JD, Dahlborg EJ, Goodwin MJ, Cade L, Zhang F, Cifuentes D,
            Curtin SJ, Blackburn JS, Thibodeau-Beganny S, Qi Y, Pierick CJ,
            Hoffman E, Maeder ML, Khayter C, Reyon D, Dobbs D, Langenau DM,
            Stupar RM, Giraldez AJ, Voytas DF, Peterson RT, Yeh JR and Joung
            JK.
  TITLE     Selection-free zinc-finger-nuclease engineering by
            context-dependent assembly (CoDA)
  JOURNAL   Nat Methods 8 (1), 67-69 (2011)
   PUBMED   21151135
REFERENCE   8  (bases 1 to 2986)
  AUTHORS   Liu W, Guan Y and Collodi P.
  TITLE     A zebrafish cell culture assay for the identification of microRNA
            targets
  JOURNAL   Zebrafish 7 (4), 343-348 (2010)
   PUBMED   21158564
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JX448391.1, FQ323156.7 and CR762469.15.
            
            On Nov 23, 2015 this sequence version replaced XM_694292.7.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: JX448391.1 [ECO:0000332]
            RNAseq introns              :: mixed/partial sample support
                                           SAMEA2168446, SAMEA2168447
                                           [ECO:0000350]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-171               JX448391.1         1-171
            172-172             FQ323156.7         68144-68144
            173-340             JX448391.1         173-340
            341-341             FQ323156.7         68313-68313
            342-800             JX448391.1         342-800
            801-805             FQ323156.7         97816-97820
            806-1146            JX448391.1         806-1146
            1147-1147           CR762469.15        50361-50361         c
            1148-1506           JX448391.1         1148-1506
            1507-1600           CR762469.15        45828-45921         c
            1601-1734           CR762469.15        44354-44487         c
            1735-1919           CR762469.15        39941-40125         c
            1920-2397           JX448391.1         1920-2397
            2398-2500           CR762469.15        32089-32191         c
            2501-2730           JX448391.1         2501-2730
            2731-2746           CR762469.15        29110-29125         c
            2747-2986           JX448391.1         2747-2986
FEATURES             Location/Qualifiers
     source          1..2986
                     /organism="Danio rerio"
                     /mol_type="mRNA"
                     /strain="AB"
                     /db_xref="taxon:7955"
                     /chromosome="16"
                     /map="16"
     gene            1..2986
                     /gene="ago1"
                     /gene_synonym="si:ch211-119n15.3"
                     /note="argonaute RISC component 1"
                     /db_xref="GeneID:570775"
                     /db_xref="ZFIN:ZDB-GENE-110606-3"
     exon            1..359
                     /gene="ago1"
                     /gene_synonym="si:ch211-119n15.3"
                     /inference="alignment:Splign:2.1.0"
     misc_feature    308..310
                     /gene="ago1"
                     /gene_synonym="si:ch211-119n15.3"
                     /note="upstream in-frame stop codon"
     CDS             335..2911
                     /gene="ago1"
                     /gene_synonym="si:ch211-119n15.3"
                     /note="eif2c1; argonaute RISC catalytic component 1"
                     /codon_start=1
                     /product="protein argonaute-1"
                     /protein_id="NP_001304152.1"
                     /db_xref="GeneID:570775"
                     /db_xref="ZFIN:ZDB-GENE-110606-3"
                     /translation="

MEAGPSGAVPVGPYPPPLQQVFQAPRRPGMGTVGKPIKLLANYFEVEIPKMDVFHYEVDIKPDKCPRRVNREVVEYMVQHFKPQLFGDRKPVYDGKKNIYTVLALPIGSEKVDFEVTIPGEGKDRIFKVSIRWLAKVSWRLLQETLVSGRLQVPLESVQALDVAMRHLASMRYTPVGRSFFSPPEGYYHPLGGGREVWFGFHQSVRPAMWKMMLNIDVSATAFYKAQPVIEFMCEVLDIRNIDEQPKTLTDSQRVRFTKEIKGLKVEVTHCGQMKRKYRVCNVTRRPASHQTFPLQLESGQTVECTVAQYFKQKYNLQLKYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIKATARSAPDRQEEISRLMKNANFNLDPYIQEFGIKVKDDMTEVTGRVLPAPILQYGGRNRAIATPNQGVWDMRGKQFYNGIEIKVWAIACFAPQKQCREEVLKNFTDQLRKISKDAGMPIQGQPCFCKYAQGADSVEPMFRHLKNTYSGLQLIIVILPGKTPVYAEVKRVGDTLLGMATQCVQVKNVVKTSPQTLSNLCLKINVKLGGINNILVPHQRSAVFQQPVIFLGADVTHPPAGDGKKPSITAVVGSMDAHPSRYCATVRVQRPRQEIIEDLSYMVRELLIQFYKSTRFKPTRIIFYRDGVPEGQLPQILHYELLAIRDACIKLEKDYQPGITYIVVQKRHHTRLFCADKSERIGKSGNIPAGTTVDTSITHPFEFDFYLCSHAGIQGTSRPSHYYVLWDDNRFTADELQILTYQLCHTYVRCTRSVSIPAPAYYARLVAFRARYHLVDKEHDSGEGSHVSGQSNGRDPQALAKAVQIHHDSLRTMYFA"

     misc_feature    335..2905
                     /gene="ago1"
                     /gene_synonym="si:ch211-119n15.3"
                     /note="protein argonaute; Provisional; Region: PLN03202"
                     /db_xref="CDD:215631"
     misc_feature    1505..2782
                     /gene="ago1"
                     /gene_synonym="si:ch211-119n15.3"
                     /note="PIWI domain, Argonaute-like subfamily. Argonaute is
                     the central component of the RNA-induced silencing complex
                     (RISC) and related complexes. The PIWI domain is the
                     C-terminal portion of Argonaute and consists of two
                     subdomains, one of which provides the...; Region:
                     Piwi_ago-like; cd04657"
                     /db_xref="CDD:240015"
     misc_feature    order(1916..1918,1928..1930,1964..1975,1982..1984,
                     2006..2008,2015..2017,2027..2029,2039..2041)
                     /gene="ago1"
                     /gene_synonym="si:ch211-119n15.3"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240015"
     misc_feature    order(2120..2122,2126..2128,2336..2338,2750..2752)
                     /gene="ago1"
                     /gene_synonym="si:ch211-119n15.3"
                     /note="active site"
                     /db_xref="CDD:240015"
     exon            360..546
                     /gene="ago1"
                     /gene_synonym="si:ch211-119n15.3"
                     /inference="alignment:Splign:2.1.0"
     exon            547..667
                     /gene="ago1"
                     /gene_synonym="si:ch211-119n15.3"
                     /inference="alignment:Splign:2.1.0"
     exon            668..849
                     /gene="ago1"
                     /gene_synonym="si:ch211-119n15.3"
                     /inference="alignment:Splign:2.1.0"
     exon            850..986
                     /gene="ago1"
                     /gene_synonym="si:ch211-119n15.3"
                     /inference="alignment:Splign:2.1.0"
     exon            987..1121
                     /gene="ago1"
                     /gene_synonym="si:ch211-119n15.3"
                     /inference="alignment:Splign:2.1.0"
     exon            1122..1209
                     /gene="ago1"
                     /gene_synonym="si:ch211-119n15.3"
                     /inference="alignment:Splign:2.1.0"
     exon            1210..1357
                     /gene="ago1"
                     /gene_synonym="si:ch211-119n15.3"
                     /inference="alignment:Splign:2.1.0"
     exon            1358..1477
                     /gene="ago1"
                     /gene_synonym="si:ch211-119n15.3"
                     /inference="alignment:Splign:2.1.0"
     exon            1478..1600
                     /gene="ago1"
                     /gene_synonym="si:ch211-119n15.3"
                     /inference="alignment:Splign:2.1.0"
     exon            1601..1734
                     /gene="ago1"
                     /gene_synonym="si:ch211-119n15.3"
                     /inference="alignment:Splign:2.1.0"
     exon            1735..1919
                     /gene="ago1"
                     /gene_synonym="si:ch211-119n15.3"
                     /inference="alignment:Splign:2.1.0"
     exon            1920..2079
                     /gene="ago1"
                     /gene_synonym="si:ch211-119n15.3"
                     /inference="alignment:Splign:2.1.0"
     exon            2080..2170
                     /gene="ago1"
                     /gene_synonym="si:ch211-119n15.3"
                     /inference="alignment:Splign:2.1.0"
     exon            2171..2365
                     /gene="ago1"
                     /gene_synonym="si:ch211-119n15.3"
                     /inference="alignment:Splign:2.1.0"
     exon            2366..2500
                     /gene="ago1"
                     /gene_synonym="si:ch211-119n15.3"
                     /inference="alignment:Splign:2.1.0"
     exon            2501..2602
                     /gene="ago1"
                     /gene_synonym="si:ch211-119n15.3"
                     /inference="alignment:Splign:2.1.0"
     exon            2603..2802
                     /gene="ago1"
                     /gene_synonym="si:ch211-119n15.3"
                     /inference="alignment:Splign:2.1.0"
     exon            2803..2986
                     /gene="ago1"
                     /gene_synonym="si:ch211-119n15.3"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      

ctgagggggggtgacggacgggtcgagcgttttgttccgctggtgaatcctgcttgaacggaaagcaggagacacaacaacccgacacacagaagccagaccagccactgacgaaagtgcacggaggagcgggtctatataaataggaactacggagcttttgtttctctctcaactcctgcgggtttgggggctccagcggcggcggcggagaagatcccgctcgcctagggaaggaggctggaagtcggggacgcttccagcctgcagggccaggcgccgtggcgattgagtatctggactttggtgaacccttaggggctcagtggatgggatggaggcgggaccatctggcgctgtgcccgtcgggccgtaccctccgcccctgcagcaggtgtttcaggcgccccgcaggcccggcatgggcaccgtcggcaaacccatcaaactgctggcgaactacttcgaggtggagatccccaaaatggacgtgtttcactatgaggtggacatcaagcctgataagtgccctcgacgggtcaacagggaggttgttgaatatatggtccagcatttcaagccgcagctcttcggtgaccgaaagccagtgtatgatgggaagaagaatatctacactgttttagcgttacctataggcagtgaaaaggttgattttgaggtgaccattcctggcgaggggaaggatcgaatctttaaggtgtcgatccgctggttggcgaaggtgtcctggaggctcctgcaggagacgctggtcagtgggcgactgcaggtcccgctggagtccgttcaggctctcgacgtggccatgagacacttggcctccatgaggtacactccagtgggccgctccttcttctcgcctcctgaaggttactaccatcctctaggtggcggcagagaggtgtggttcggcttccatcagtccgttcgacccgccatgtggaagatgatgctcaacattgatgtatctgcaacagccttttataaagctcagcctgtcattgagttcatgtgtgaagtcctggacatccgtaacattgatgagcagcccaagacactaactgactcgcagagggtccggttcaccaaggagatcaaaggcctgaaggtggaggttacgcactgtggtcaaatgaaaaggaagtaccgggtctgtaacgtcacacggcgtcctgctagtcaccaaacgtttcctctacagctggagagcggacagacggtagaatgcacagtagctcaatatttcaagcagaagtacaacctgcagctcaagtaccctcacctgccctgtctacaggtgggacaggagcagaaacacacatacctgcctctggaggtttgcaatattgtagcagggcagcggtgcatcaagaaactgacagataatcagacgtccaccatgatcaaagccacggcgcgctccgcaccagaccgacaggaggagatcagcagactgatgaagaacgctaactttaatctggatccttacattcaagagtttgggattaaggtgaaggacgacatgacggaggtgaccggcagagtccttccagccccaatcctgcagtatggcggtcggaatcgtgccatcgcgactccaaaccagggtgtgtgggacatgaggggaaaacagttctataatggcatcgagatcaaggtttgggccattgcttgcttcgctccgcagaaacagtgtcgggaagaggtgctcaagaacttcaccgaccagctgcgcaagatctcaaaggatgctgggatgccgattcagggtcagccgtgtttctgtaagtacgctcagggggccgacagcgtggagcccatgttcagacacctgaagaacacttactctggactgcagctcatcattgtcatcctgcctgggaaaacccccgtctacgcggaagtgaagcgtgttggagacactcttctaggaatggccactcagtgtgtgcaggtgaagaacgtggtaaaaacctccccgcagacgctctccaacctctgcctcaaaatcaacgtcaaactcggcggcatcaacaacatcctcgtgccgcatcaacggtcagcagtgtttcagcagcccgtgatcttcctcggggcagatgttacacacccgccggctggtgatgggaagaagccatctattactgctgttgttggcagtatggacgctcatcccagcagatactgcgctacagttcgagtccagcggcccagacaggagattattgaagatctgtcctacatggtgagagaattactgatccagttctacaagtccacccgcttcaagcccacgagaatcatcttctacagggacggcgtaccagaaggacagctgccacaaatcctgcactatgagctgttggccatcagagatgcctgcattaagctggagaaagactatcagcccggcatcacttacatagtcgtgcagaaacgccaccatacacgactcttctgcgccgataaatctgaacgaatcggcaagagcgggaacataccagcgggaactacggtcgacaccagcatcacacatccattcgagtttgacttctacctgtgcagtcatgcaggcattcagggcaccagccgaccctcacactactacgtcctgtgggacgacaatcgctttacggctgacgagctgcagattctcacctaccagctgtgccacacgtatgtgcgctgcacccgctccgtctccatccccgcgccagcatactacgccagactcgtggctttccgggcccgataccatctggtggacaaagaacatgacagcggggagggaagtcacgtctccgggcagagcaatggtcgagatcctcaagcgttggccaaagcggtgcagattcaccacgactccctgaggaccatgtattttgcctgaataacaaaaagcgcacgtctccggacacctcgagccagaacccctgggtgctaaaccagtccaatgaagcccaca

//