LOCUS       NM_001252559            2729 bp    mRNA    linear   VRT 08-OCT-2023
DEFINITION  Danio rerio argonaute RISC component 4 (ago4), mRNA.
ACCESSION   NM_001252559 XM_003200646
VERSION     NM_001252559.2
KEYWORDS    RefSeq.
SOURCE      Danio rerio (zebrafish)
  ORGANISM  Danio rerio
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Ostariophysi;
            Cypriniformes; Danionidae; Danioninae; Danio.
REFERENCE   1  (bases 1 to 2729)
  AUTHORS   Raman R, Ryon M and Jagadeeswaran P.
  TITLE     RNaseH-mediated simultaneous piggyback knockdown of multiple genes
            in adult zebrafish
  JOURNAL   Sci Rep 10 (1), 20187 (2020)
   PUBMED   33214638
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2729)
  AUTHORS   Liu Y, Zhu Z, Ho IHT, Shi Y, Li J, Wang X, Chan MTV and Cheng CHK.
  TITLE     Genetic Deletion of miR-430 Disrupts Maternal-Zygotic Transition
            and Embryonic Body Plan
  JOURNAL   Front Genet 11, 853 (2020)
   PUBMED   32849832
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2729)
  AUTHORS   Katz S, Cussigh D, Urban N, Blomfield I, Guillemot F, Bally-Cuif L
            and Coolen M.
  TITLE     A Nuclear Role for miR-9 and Argonaute Proteins in Balancing
            Quiescent and Activated Neural Stem Cell States
  JOURNAL   Cell Rep 17 (5), 1383-1398 (2016)
   PUBMED   27783951
REFERENCE   4  (bases 1 to 2729)
  AUTHORS   McFarlane L, Svingen T, Braasch I, Koopman P, Schartl M and Wilhelm
            D.
  TITLE     Expansion of the Ago gene family in the teleost clade
  JOURNAL   Dev Genes Evol 221 (2), 95-104 (2011)
   PUBMED   21556854
REFERENCE   5  (bases 1 to 2729)
  AUTHORS   Cifuentes D, Xue H, Taylor DW, Patnode H, Mishima Y, Cheloufi S, Ma
            E, Mane S, Hannon GJ, Lawson ND, Wolfe SA and Giraldez AJ.
  TITLE     A novel miRNA processing pathway independent of Dicer requires
            Argonaute2 catalytic activity
  JOURNAL   Science 328 (5986), 1694-1698 (2010)
   PUBMED   20448148
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JX448395.1.
            
            On Dec 24, 2014 this sequence version replaced NM_001252559.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: JX448395.1 [ECO:0000332]
            RNAseq introns              :: mixed/partial sample support
                                           SAMEA2168446, SAMEA2168447
                                           [ECO:0000350]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2729              JX448395.1         1-2729
FEATURES             Location/Qualifiers
     source          1..2729
                     /organism="Danio rerio"
                     /mol_type="mRNA"
                     /strain="AB"
                     /db_xref="taxon:7955"
                     /chromosome="19"
                     /map="19"
     gene            1..2729
                     /gene="ago4"
                     /gene_synonym="eif2c4; fd14f04; wu:fd14f04"
                     /note="argonaute RISC component 4"
                     /db_xref="GeneID:100535764"
                     /db_xref="ZFIN:ZDB-GENE-030131-5357"
     exon            1..142
                     /gene="ago4"
                     /gene_synonym="eif2c4; fd14f04; wu:fd14f04"
                     /inference="alignment:Splign:2.1.0"
     CDS             124..2715
                     /gene="ago4"
                     /gene_synonym="eif2c4; fd14f04; wu:fd14f04"
                     /note="argonaute 4; eukaryotic translation initiation
                     factor 2C, 4; argonaute RISC catalytic component 4"
                     /codon_start=1
                     /product="protein argonaute-4"
                     /protein_id="NP_001239488.1"
                     /db_xref="GeneID:100535764"
                     /db_xref="ZFIN:ZDB-GENE-030131-5357"
                     /translation="

MEALGPGPPAPTSLFQPPRRPGLGTVGKPIRLLANHFQVQIPKIDVYHYDIDIKPEKRPRRVNREVVDTMVRHFKMQIFGDRQPGYDGKRNMYTAHPLPIGRDRVDLEVTLPGEGKDQTFKVSLQWVSVVSLQMLLEALSGHLNEVPEDSVQALDVITRHLPSMRYTPVGRSFFSPPEGYYHPLGGGREVWFGFHQSVRPAMWNMMLNIDVSATAFYRAQPVIEFMCEVLDIQNINEQTKPLTDSQRVKFTKEIRGLKVEVTHCGQMKRKYRVCNVTRRPASHQTFPLQLENGQAMECTVAQYFKQKYSLQLKYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIKATARSAPDRQEEISRLVKSNSMVGGPDPYLKEFGIVVHNDMTEVTGRVLPAPMLQYGGRNKTVATPNQGVWDMRGKQFYAGIEIKVWAVACFAPQKQCREDLLKSFTDQLRKISKDAGMPIQGQPCFCKYAQGADSVEPMFKHLKMSYVGLQLIVVILPGKTPVYAEVKRVGDTLLGMATQCVQVKNVVKTSPQTLSNLCLKINAKLGGINNVLVPHQRPSVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDGHPSRYCATVRVQTSRQDLSQEQLFSQEVIQDLTNMVRELLIQFYKSTRFKPTRIIYYRGGVSEGQMKQVAWPELIAIRKACISLEEDYRPGITYIVVQKRHHTRLFCSDKAERVGKSGNVPAGTTVDSTITHPSEFDFYLCSHAGIQGTSRPSHYHVLWDDNCFTADELQLLTYQLCHTYVRCTRSVSIPAPAYYARLVAFRARYHLVDKDHDSAEGSHVSGQSNGRDPQALAKAVQIHYDTQHTMYFA"

     misc_feature    205..594
                     /gene="ago4"
                     /gene_synonym="eif2c4; fd14f04; wu:fd14f04"
                     /note="N-terminal domain of argonaute; Region: ArgoN;
                     pfam16486"
                     /db_xref="CDD:435368"
     misc_feature    625..777
                     /gene="ago4"
                     /gene_synonym="eif2c4; fd14f04; wu:fd14f04"
                     /note="Argonaute linker 1 domain; Region: ArgoL1;
                     pfam08699"
                     /db_xref="CDD:430160"
     misc_feature    778..1140
                     /gene="ago4"
                     /gene_synonym="eif2c4; fd14f04; wu:fd14f04"
                     /note="PAZ domain, argonaute_like subfamily. Argonaute is
                     part of the RNA-induced silencing complex (RISC), and is
                     an endonuclease that plays a key role in the RNA
                     interference pathway. The PAZ domain has been named after
                     the proteins Piwi,Argonaut, and Zwille; Region:
                     PAZ_argonaute_like; cd02846"
                     /db_xref="CDD:239212"
     misc_feature    order(934..936,979..981,1021..1023,1033..1035,1087..1089,
                     1108..1110,1114..1116)
                     /gene="ago4"
                     /gene_synonym="eif2c4; fd14f04; wu:fd14f04"
                     /note="nucleic acid-binding interface [nucleotide
                     binding]; other site"
                     /db_xref="CDD:239212"
     misc_feature    1279..2586
                     /gene="ago4"
                     /gene_synonym="eif2c4; fd14f04; wu:fd14f04"
                     /note="PIWI domain, Argonaute-like subfamily. Argonaute is
                     the central component of the RNA-induced silencing complex
                     (RISC) and related complexes. The PIWI domain is the
                     C-terminal portion of Argonaute and consists of two
                     subdomains, one of which provides the...; Region:
                     Piwi_ago-like; cd04657"
                     /db_xref="CDD:240015"
     misc_feature    order(1690..1692,1702..1704,1738..1749,1756..1758,
                     1780..1782,1789..1791,1801..1803,1813..1815)
                     /gene="ago4"
                     /gene_synonym="eif2c4; fd14f04; wu:fd14f04"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240015"
     misc_feature    order(1894..1896,1900..1902,2140..2142,2554..2556)
                     /gene="ago4"
                     /gene_synonym="eif2c4; fd14f04; wu:fd14f04"
                     /note="active site"
                     /db_xref="CDD:240015"
     exon            143..314
                     /gene="ago4"
                     /gene_synonym="eif2c4; fd14f04; wu:fd14f04"
                     /inference="alignment:Splign:2.1.0"
     exon            315..435
                     /gene="ago4"
                     /gene_synonym="eif2c4; fd14f04; wu:fd14f04"
                     /inference="alignment:Splign:2.1.0"
     exon            436..617
                     /gene="ago4"
                     /gene_synonym="eif2c4; fd14f04; wu:fd14f04"
                     /inference="alignment:Splign:2.1.0"
     exon            618..754
                     /gene="ago4"
                     /gene_synonym="eif2c4; fd14f04; wu:fd14f04"
                     /inference="alignment:Splign:2.1.0"
     exon            755..889
                     /gene="ago4"
                     /gene_synonym="eif2c4; fd14f04; wu:fd14f04"
                     /inference="alignment:Splign:2.1.0"
     exon            890..977
                     /gene="ago4"
                     /gene_synonym="eif2c4; fd14f04; wu:fd14f04"
                     /inference="alignment:Splign:2.1.0"
     exon            978..1125
                     /gene="ago4"
                     /gene_synonym="eif2c4; fd14f04; wu:fd14f04"
                     /inference="alignment:Splign:2.1.0"
     exon            1126..1245
                     /gene="ago4"
                     /gene_synonym="eif2c4; fd14f04; wu:fd14f04"
                     /inference="alignment:Splign:2.1.0"
     exon            1246..1374
                     /gene="ago4"
                     /gene_synonym="eif2c4; fd14f04; wu:fd14f04"
                     /inference="alignment:Splign:2.1.0"
     exon            1375..1508
                     /gene="ago4"
                     /gene_synonym="eif2c4; fd14f04; wu:fd14f04"
                     /inference="alignment:Splign:2.1.0"
     exon            1509..1693
                     /gene="ago4"
                     /gene_synonym="eif2c4; fd14f04; wu:fd14f04"
                     /inference="alignment:Splign:2.1.0"
     exon            1694..1853
                     /gene="ago4"
                     /gene_synonym="eif2c4; fd14f04; wu:fd14f04"
                     /inference="alignment:Splign:2.1.0"
     exon            1854..2169
                     /gene="ago4"
                     /gene_synonym="eif2c4; fd14f04; wu:fd14f04"
                     /inference="alignment:Splign:2.1.0"
     exon            2170..2304
                     /gene="ago4"
                     /gene_synonym="eif2c4; fd14f04; wu:fd14f04"
                     /inference="alignment:Splign:2.1.0"
     exon            2305..2406
                     /gene="ago4"
                     /gene_synonym="eif2c4; fd14f04; wu:fd14f04"
                     /inference="alignment:Splign:2.1.0"
     exon            2407..2606
                     /gene="ago4"
                     /gene_synonym="eif2c4; fd14f04; wu:fd14f04"
                     /inference="alignment:Splign:2.1.0"
     exon            2607..2729
                     /gene="ago4"
                     /gene_synonym="eif2c4; fd14f04; wu:fd14f04"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      

cacatctacgcatcccttccatttcttaaattagccaccttaagcttcgagataccgcccccaatttcagtgtcggtacggagccgccaggccgggacagagacggagagagagccctcggccatggaagcgctcggacccggcccgcctgcccctacctccctcttccagccaccgcgccggcccggcttgggtacggtggggaagccgattcgcctgctggccaatcacttccaggtgcagatccccaagatcgacgtctaccattatgacatcgacatcaaacctgaaaaacggccccgcagggtcaacagagaggtggtggacacaatggttagacattttaagatgcagatctttggggaccggcagcctggatatgatggaaagaggaacatgtacacggcacatccgctacccatcgggagggacagggtggatctggaggtgacgctgccgggtgaagggaaggatcagacttttaaggtgtctctgcagtgggtgtcggtggtcagtctgcagatgctactggaagctctgtctggtcacctgaacgaggtgccggaggattcagttcaggctctggacgtcatcactcgtcacctgccttccatgcggtacactccagtaggccggtccttcttctctccaccagagggctactatcatccactgggaggagggagggaggtgtggttcggtttccatcagtctgtccgtcctgccatgtggaacatgatgctcaacatagatgtgtcagcaacagcgttttacagagctcagcctgtcatcgaattcatgtgtgaggttttggacatccagaacatcaacgagcagaccaaacccctcacagattcacaacgtgtcaagttcaccaaggagatccgaggactgaaagtggaggtcacacactgcggccagatgaagaggaagtaccgtgtgtgtaatgtcacgcgccgcccagccagtcatcagacgtttcctttgcagcttgagaatggacaagctatggagtgcaccgttgcccagtatttcaaacaaaagtacagcctgcagctcaaatacccccacctgccctgcctccaggtgggacaggagcagaagcacacgtaccttcccctggaggtttgtaatatagtggcaggacagcgctgcataaagaaactaacagataatcagacctccaccatgatcaaagctacggcccgctcagccccagacagacaggaggagatcagccgactggtcaaaagcaacagtatggttggcgggcccgacccttatctgaaagagtttggcattgtggtgcacaatgacatgaccgaggtgacagggcgggtcctcccagcgcccatgctgcagtatgggggccggaataagacggtggccacacccaaccagggcgtctgggacatgagggggaagcagttttatgccggaatagagatcaaggtttgggccgtggcctgcttcgcccctcagaaacaatgcagagaggacctgctcaagagtttcactgaccagctgcgcaagatttccaaggatgctgggatgccgatccaaggccagccgtgcttctgcaaatacgcccagggtgcagacagtgtggagcccatgttcaagcatctcaagatgtcttacgtgggcctgcagctcatcgtggtcatcctgcccggaaaaacacctgtctatgcggaggtgaagcgtgtgggagacactctcctgggaatggccactcagtgtgttcaggtgaagaacgtggtaaaaacgtctcctcagacgctctccaacctctgtctgaagatcaacgccaaactaggaggcatcaacaatgtgctggtgccacatcagaggccttctgtgttccagcagccggtgatcttcttgggtgcggatgtcactcaccctccagcgggcgatgggaagaagccgtccatcgctgcagtggtaggtagtatggacggacacccgagccgatactgcgccaccgtacgggtgcaaacgtcccgccaggatctgtcccaggagcagctcttcagccaggaggtcatccaggatctcaccaacatggtgcgcgagctgctcattcagttctacaaatccacccgattcaagcccacgcgcattatctactaccgcggaggagtttctgagggccagatgaagcaggtggcgtggccagagctgatcgccatcaggaaagcctgcatcagtctagaagaggattacaggccgggaatcacatacatcgtagtgcagaaacgccaccacactcgactcttctgctccgacaaagccgagagggttgggaagagtggcaacgtcccagcaggcactacggtggacagcaccatcacacacccctcagagtttgacttctacctgtgcagccatgctggcatccagggcaccagccgtccctctcactatcacgtgttgtgggacgacaactgtttcaccgctgacgagctccagcttttgacctatcagctctgccacacttatgtgcgctgcacccgctccgtctccatccccgcgccagcttactatgcccggctagtggccttccgtgcacgctatcatttagtggacaaagaccatgacagtgctgaaggcagtcacgtatcaggacagagcaacggtcgagacccgcaggcgctggcgaaagctgtgcagattcactacgacacacagcacaccatgtacttcgcctgaccacagccagcagg

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