LOCUS       NM_128424               2162 bp    mRNA    linear   PLN 20-OCT-2022
DEFINITION  Arabidopsis thaliana 3-ketoacyl-CoA synthase 12 (KCS12), mRNA.
ACCESSION   NM_128424
VERSION     NM_128424.3
DBLINK      BioProject: PRJNA116
            BioSample: SAMN03081427
KEYWORDS    RefSeq.
SOURCE      Arabidopsis thaliana (thale cress)
  ORGANISM  Arabidopsis thaliana
            Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
            Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae;
            Pentapetalae; rosids; malvids; Brassicales; Brassicaceae;
            Camelineae; Arabidopsis.
REFERENCE   1  (bases 1 to 2162)
  AUTHORS   Lin,X., Kaul,S., Rounsley,S., Shea,T.P., Benito,M.I., Town,C.D.,
            Fujii,C.Y., Mason,T., Bowman,C.L., Barnstead,M., Feldblyum,T.V.,
            Buell,C.R., Ketchum,K.A., Lee,J., Ronning,C.M., Koo,H.L.,
            Moffat,K.S., Cronin,L.A., Shen,M., Pai,G., Van Aken,S., Umayam,L.,
            Tallon,L.J., Gill,J.E., Adams,M.D., Carrera,A.J., Creasy,T.H.,
            Goodman,H.M., Somerville,C.R., Copenhaver,G.P., Preuss,D.,
            Nierman,W.C., White,O., Eisen,J.A., Salzberg,S.L., Fraser,C.M. and
            Venter,J.C.
  TITLE     Sequence and analysis of chromosome 2 of the plant Arabidopsis
            thaliana
  JOURNAL   Nature 402 (6763), 761-768 (1999)
   PUBMED   10617197
REFERENCE   2  (bases 1 to 2162)
  CONSRTM   NCBI Genome Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-OCT-2022) National Center for Biotechnology
            Information, NIH, Bethesda, MD 20894, USA
REFERENCE   3  (bases 1 to 2162)
  AUTHORS   Krishnakumar,V., Cheng,C.-Y., Chan,A.P., Schobel,S., Kim,M.,
            Ferlanti,E.S., Belyaeva,I., Rosen,B.D., Micklem,G., Miller,J.R.,
            Vaughn,M. and Town,C.D.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAY-2016) Plant Genomics, J. Craig Venter Institute,
            9704 Medical Center Dr, Rockville, MD 20850, USA
  REMARK    Protein update by submitter
REFERENCE   4  (bases 1 to 2162)
  AUTHORS   Swarbreck,D., Lamesch,P., Wilks,C. and Huala,E.
  CONSRTM   TAIR
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2011) Department of Plant Biology, Carnegie
            Institution, 260 Panama Street, Stanford, CA, USA
COMMENT     REVIEWED REFSEQ: This record has been curated by TAIR and Araport.
            This record is derived from an annotated genomic sequence
            (NC_003071).
            
            On Sep 12, 2016 this sequence version replaced NM_128424.2.
FEATURES             Location/Qualifiers
     source          1..2162
                     /organism="Arabidopsis thaliana"
                     /mol_type="mRNA"
                     /db_xref="taxon:3702"
                     /chromosome="2"
                     /ecotype="Columbia"
     gene            1..2162
                     /gene="KCS12"
                     /locus_tag="AT2G28630"
                     /gene_synonym="3-ketoacyl-CoA synthase 12; T8O18.8;
                     T8O18_8"
                     /note="Encodes KCS12, a member of the 3-ketoacyl-CoA
                     synthase family involved in the biosynthesis of VLCFA
                     (very long chain fatty acids)."
                     /db_xref="Araport:AT2G28630"
                     /db_xref="GeneID:817412"
                     /db_xref="TAIR:AT2G28630"
     CDS             269..1699
                     /gene="KCS12"
                     /locus_tag="AT2G28630"
                     /gene_synonym="3-ketoacyl-CoA synthase 12; T8O18.8;
                     T8O18_8"
                     /codon_start=1
                     /product="3-ketoacyl-CoA synthase 12"
                     /protein_id="NP_180431.1"
                     /db_xref="Araport:AT2G28630"
                     /db_xref="GeneID:817412"
                     /db_xref="TAIR:AT2G28630"
                     /translation="

MDLLFLFFSLLLSYLFFKIWKLIDSKQDKDCYILDYQCHKPTDDRMVSTQFSGEIIYRNQNLGLTEYKFLLKAIVSSGIGEQTYAPRLVFEGREERPSLQDGISEMEEFYVDSIGKLLERNQISPKDIDILVVNVSMLSSTPSLASRIINHYKMRDDVKVFNLTGMGCSASLISVDIVKNIFKSYANKLALVATSESLSPNWYSGNNRSMILANCLFRSGGCAILLTNKRSLRKKAMFKLKCMVRTHHGAREESYNCCIQAEDEQGRVGFYLGKNLPKAATRAFVENLKVITPKILPVTELIRFMLKLLIKKIKIRQNPSKGSTNLPPGTPLKAGINFKTGIEHFCIHTGGKAVIDGIGHSLDLNEYDIEPARMTLHRFGNTSASSLWYVLAYMEAKKRLKRGDRVFMISFGAGFKCNSCVWEVVRDLTGGESKGNVWNHCIDDYPPKSILNPYLEKFGWIQDEDPDTFKVPDAFM"

     misc_feature    335..1183
                     /gene="KCS12"
                     /locus_tag="AT2G28630"
                     /gene_synonym="3-ketoacyl-CoA synthase 12; T8O18.8;
                     T8O18_8"
                     /note="FAE1/Type III polyketide synthase-like protein;
                     Region: FAE1_CUT1_RppA; pfam08392"
                     /db_xref="CDD:429970"
     misc_feature    order(422..424,431..436,917..919,1136..1138,1319..1327)
                     /gene="KCS12"
                     /locus_tag="AT2G28630"
                     /gene_synonym="3-ketoacyl-CoA synthase 12; T8O18.8;
                     T8O18_8"
                     /note="malonyl-CoA binding site [chemical binding]; other
                     site"
                     /db_xref="CDD:238427"
     misc_feature    order(551..556,563..565,686..691,704..706,716..718,
                     734..754,764..769,794..799,806..808,815..823,1028..1030,
                     1034..1042,1076..1087,1514..1516)
                     /gene="KCS12"
                     /locus_tag="AT2G28630"
                     /gene_synonym="3-ketoacyl-CoA synthase 12; T8O18.8;
                     T8O18_8"
                     /note="dimer interface [polypeptide binding]; other site"
                     /db_xref="CDD:238427"
     misc_feature    order(770..772,1310..1312,1409..1411)
                     /gene="KCS12"
                     /locus_tag="AT2G28630"
                     /gene_synonym="3-ketoacyl-CoA synthase 12; T8O18.8;
                     T8O18_8"
                     /note="active site"
                     /db_xref="CDD:238427"
     misc_feature    order(854..862,920..922,1070..1078,1133..1138,1415..1417,
                     1505..1507)
                     /gene="KCS12"
                     /locus_tag="AT2G28630"
                     /gene_synonym="3-ketoacyl-CoA synthase 12; T8O18.8;
                     T8O18_8"
                     /note="product binding site [active]"
                     /db_xref="CDD:238427"
     misc_feature    1265..1537
                     /gene="KCS12"
                     /locus_tag="AT2G28630"
                     /gene_synonym="3-ketoacyl-CoA synthase 12; T8O18.8;
                     T8O18_8"
                     /note="Beta-ketoacyl synthase, C-terminal domain; Region:
                     Ketoacyl-synt_C; cl21486"
                     /db_xref="CDD:451266"
ORIGIN      

acttgttatatcttaccgtcaacgaaaatttattaaaattccctttcttcaaaaatactattaaaactcaatggttatgtagaacccactgggaaattcagagctggcgtttcctcatccattctcatatattctcttatataatcccctttacaatctccacttcatttccatgcatcttactcacttcatcatctcttcttaaaccccaaaacaaacaaacaaacaaacaaaaaataccaaaacaaagaagaaaaaaaaaataactatggatcttctctttctctttttctctcttctcctctcttacctctttttcaagatctggaaactcatagactcaaagcaagacaaagattgctacattttagactaccaatgtcataaaccaaccgacgatcgaatggtgagcactcaattcagtggagagatcatttatcgaaaccaaaaccttggtttaaccgaatacaagttccttctcaaagccatagtaagctcagggattggagaacaaacctacgctccaagacttgtctttgaaggtcgagaagagcgtccttcgttacaagatgggatctccgagatggaagagttctacgtggacagcatcggaaaactcctggagagaaatcaaatatctcctaaagacatagacatcctcgtagtcaacgtctccatgctctcttcaacgccatctttagcttcaagaatcattaaccattacaagatgagagacgacgtcaaagtcttcaacttgaccgggatgggatgcagcgcaagtctaatctccgtagacattgtcaagaacatttttaagagctacgcaaacaagttagctcttgttgccacctccgagtctttgagccctaattggtatagcggaaacaaccgttcaatgatcttagccaactgtttgttccggtccggtggatgtgctattctcttgactaacaaacgtagcttgagaaagaaagcaatgtttaaactcaagtgtatggtacggactcaccatggagctagagaggagtcttataattgttgcatccaagccgaagatgaacaaggccgtgtcgggttttacttggggaagaatctaccaaaagccgctactcgtgcttttgtagaaaacctcaaggttataacacccaagattcttcccgtaaccgagcttataaggttcatgttaaagcttcttatcaagaaaatcaagattcgtcaaaaccctagcaagggctccacgaatctcccaccggggactccattaaaggcaggaatcaacttcaagaccgggatcgaacatttttgcatacataccggaggaaaagctgtgattgatgggattggacatagcttggatttaaacgagtatgatattgaacctgcgaggatgactcttcaccggtttggcaatacttcggcgagtagtttgtggtatgtgttggcttacatggaggccaagaagagattgaagagaggagatagagtttttatgataagctttggagctggttttaagtgtaatagctgcgtttgggaagttgtgagagatcttactggtggtgaatcaaaaggaaatgtgtggaatcattgcattgatgattatccaccaaaatcgattctgaatccttatttggagaagtttggctggattcaagatgaagatcctgacactttcaaggtccctgacgctttcatgtaaaacgtttacgcacaaaaacgcaaacgcaaacgcaaaaacaacacaaggtgatacaatattctctctttcttaatttcttcttttttgcctttagttaaaatttttacgtttttttgtttaaatggattggaaaggagatatgaatgtaaaagaaactttaattagttttttttttaatatgattttttttgggtgttataatttgttaattacatactttgaaataagggttttaagggacatgcacttccaatacgatgagaatttactaatttgccatcatattggaatgctgccttctttttttcttgttaaatgttgtatcaattttatattttttgtaatgttcatttttcactctatgttgtacttgtttcgcattaactcaagcttgccactgtacatattcattctatttttctgtaacttaaaaaaaaaactatacaagaaggaaattaaggaa

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