LOCUS       XM_077298129            6316 bp    mRNA    linear   VRT 27-SEP-2025
DEFINITION  PREDICTED: Ranitomeya variabilis ATPase copper transporting beta
            (ATP7B), transcript variant X1, mRNA.
ACCESSION   XM_077298129
VERSION     XM_077298129.1
DBLINK      BioProject: PRJNA1327461
KEYWORDS    RefSeq.
SOURCE      Ranitomeya variabilis (Zimmermann's poison frog)
  ORGANISM  Ranitomeya variabilis
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Amphibia; Batrachia; Anura; Neobatrachia; Hyloidea; Dendrobatidae;
            Dendrobatinae; Ranitomeya.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135234) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051348905.1-RS_2025_09
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 09/12/2025
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6316
                     /organism="Ranitomeya variabilis"
                     /mol_type="mRNA"
                     /isolate="aRanVar5"
                     /db_xref="taxon:490064"
                     /chromosome="3"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Palo Alto, California"
                     /lat_lon="37.4275 N 122.1697 W"
                     /collection_date="2024-02-24"
                     /collected_by="Billie Goolsby"
     gene            1..6316
                     /gene="ATP7B"
                     /note="ATPase copper transporting beta; Derived by
                     automated computational analysis using gene prediction
                     method: Gnomon. Supporting evidence includes similarity
                     to: 14 Proteins, and 100% coverage of the annotated
                     genomic feature by RNAseq alignments, including 1 sample
                     with support for all annotated introns"
                     /db_xref="GeneID:143817055"
     CDS             104..4588
                     /gene="ATP7B"
                     /codon_start=1
                     /product="copper-transporting ATPase 2 isoform X1"
                     /protein_id="XP_077154244.1"
                     /db_xref="GeneID:143817055"
                     /translation="

MILAKHIFNINDTEERVKSMDNLALTDHRKSHDIIQHADPNSPDDCKKRDQSGIMTGDPGDPKEENYDILKTSHAPAPRKPRFAFDNRGYDGSLNDLTKPCLEVSSVVVGIIGMTCQSCVQSIEGRISKIPSVVNIKVYLDKANAEVCYIPSEITAQKICEEIEDMGFDTHIDICKELKGGLYKEDVVKIRVEGMTCQSCVNSIEGKIGKIQGVEKIKVSLTNQEAMIVYCPQYIQPEDLQKRIEDMGFVASVKCKPDPTKLGPINIELLQSISTDPKLRNTVGPIGNTIARTIHIEGMHCKSCVHNIEGSISELPGVQNIKVSLENKNAVISLVQDLTSLLMLKEVIEALPPGHFKVTLPEEDEPSPALRTKSTLHSSPNQIERTTTRTASIQIGGMTCGSCVSTIEDTISQRKGVQSISVSLPEGMGTILYIHTETNSGELRLAIEDMGFDATLISDSLLATSGDQLSFPVKQKLQETTKNANLHQDYIVDVLPKKPHLDIPLMENRTPQKCVLQVTGMTCASCVSNIERNLKKKDGIISVLVALMAGKAEVKYIADQIEPSEIAQLIENLGFGAVVLEDCTASHGSIELVITGMTCASCVHNIESRLIRMPGILQASVALATCKAQFKFDPEIVGPRDIIQVIEGIGFQASLSRREPSVHNLDHKEEIRQWRKSFLFSLIFGIPVICIMVYMLIANSNHHGQSALDNNIVPGLSIMNLIFFILCTLVQTFGGWYFYVQAYKSLKHKAANMDVLIVLATTIAYIYSVVILIVAIVEQSAQSPETFFDTPPMLFMFIALGRWLEHIAKSKTSEALAKLISLQATEATVVTLGPNHSVLREEQVAVDLVQRGDIVKVVPGGKFPVDGKVIEGTSMADESLITGEPMPVRKKPGSIVIAGSINAHGSVLVEATHVGSDTTLAQIVKLVEEAQMSKAPIQQLADKISGYFVPFIISISVVTLIGWTTVGFVNFDVILKYFPDYSKTISKAEIIIRVGFQTAITVLSIACPCALGLATPTAVMVGTGVAAQNGILIKGGEPLEMAHKINTVMFDKTGTITHGVPKVMRVLLLGDVVRMPLRRMLAVVGTAEASSEHPLGMAMTKYCKEELGTGSLGYCTDFQAVPGCGIRCKVNNVESVLVQSEKGLNEQNAYISGLTRPHEDGSLIIAPELQGASAPQPHSVLIGNREWMRRNGLHISSDVDEAMTSHEMKGQTAVLIAIDGALCGMIAIADTVKQEAALAVHTLKAMGVDVVLITGDNRKTAKAIATQVGINKVFAEVLPSHKVAKVEALQNEGKKVAMVGDGVNDSPALARADIGIAIGTGTDVAIEAADVVLIRNDLLDVVASIHLSKRTVRRIRINFMFALIYNLVGIPIAAGVFLPVGLVLQPWMGSAAMAASSVSVVLSSLQLKCYRKPESERYEARAQGRMKPLTPSQISVHVGMDDRRRDIPMASAWDQISYISQASSSRNTRQDSLTGDRQDKWSLLLNEAEDDLYM"

     misc_feature    440..610
                     /gene="ATP7B"
                     /note="copper chaperone CopZ; Region: chaper_CopZ_Bs;
                     NF033795"
                     /db_xref="CDD:411375"
     misc_feature    668..859
                     /gene="ATP7B"
                     /note="Heavy-metal-associated domain (HMA) is a conserved
                     domain of approximately 30 amino acid residues found in a
                     number of proteins that transport or detoxify heavy
                     metals, for example, the CPx-type heavy metal ATPases and
                     copper chaperones. HMA domain...; Region: HMA; cd00371"
                     /db_xref="CDD:238219"
     misc_feature    order(686..694,701..703)
                     /gene="ATP7B"
                     /note="metal-binding site [ion binding]"
                     /db_xref="CDD:238219"
     misc_feature    980..1156
                     /gene="ATP7B"
                     /note="Copper chaperone CopZ [Inorganic ion transport and
                     metabolism]; Region: CopZ; COG2608"
                     /db_xref="CDD:442020"
     misc_feature    1280..1468
                     /gene="ATP7B"
                     /note="Heavy-metal-associated domain (HMA) is a conserved
                     domain of approximately 30 amino acid residues found in a
                     number of proteins that transport or detoxify heavy
                     metals, for example, the CPx-type heavy metal ATPases and
                     copper chaperones. HMA domain...; Region: HMA; cd00371"
                     /db_xref="CDD:238219"
     misc_feature    order(1295..1303,1310..1312)
                     /gene="ATP7B"
                     /note="metal-binding site [ion binding]"
                     /db_xref="CDD:238219"
     misc_feature    1646..1834
                     /gene="ATP7B"
                     /note="Heavy-metal-associated domain (HMA) is a conserved
                     domain of approximately 30 amino acid residues found in a
                     number of proteins that transport or detoxify heavy
                     metals, for example, the CPx-type heavy metal ATPases and
                     copper chaperones. HMA domain...; Region: HMA; cd00371"
                     /db_xref="CDD:238219"
     misc_feature    order(1664..1672,1679..1681)
                     /gene="ATP7B"
                     /note="metal-binding site [ion binding]"
                     /db_xref="CDD:238219"
     misc_feature    1874..2065
                     /gene="ATP7B"
                     /note="Heavy-metal-associated domain (HMA) is a conserved
                     domain of approximately 30 amino acid residues found in a
                     number of proteins that transport or detoxify heavy
                     metals, for example, the CPx-type heavy metal ATPases and
                     copper chaperones. HMA domain...; Region: HMA; cd00371"
                     /db_xref="CDD:238219"
     misc_feature    order(1892..1900,1907..1909)
                     /gene="ATP7B"
                     /note="metal-binding site [ion binding]"
                     /db_xref="CDD:238219"
     misc_feature    2129..4267
                     /gene="ATP7B"
                     /note="P-type heavy metal-transporting ATPase, similar to
                     human copper-transporting ATPases, ATP7A and ATP7B;
                     Region: P-type_ATPase_Cu-like; cd02094"
                     /db_xref="CDD:319783"
     misc_feature    order(3122..3124,3128..3130,4196..4198)
                     /gene="ATP7B"
                     /note="putative Cu binding site [ion binding]; other site"
                     /db_xref="CDD:319783"
     misc_feature    order(3254..3262,3464..3466,3647..3655,3743..3745,
                     3863..3871,3929..3931,3938..3940,3947..3949,4004..4006,
                     4013..4015)
                     /gene="ATP7B"
                     /note="putative ATP binding site [chemical binding]; other
                     site"
                     /db_xref="CDD:319783"
ORIGIN      

aggattctgctcattgctgcatcccttttacatggattatattttggatgtctcatccacagttttgtattaatttcactgcccttgtaaaattattcttaaaatgattttggcaaagcacatttttaacatcaatgacacggaagaaagggttaaaagtatggataatctagctctgaccgaccataggaagtctcatgatattattcagcatgcagatcctaactctccagatgattgcaagaaaagggatcaatcgggaataatgaccggagatcctggagaccccaaggaagagaactatgacattctcaagacaagccatgcaccagcaccaagaaaaccaagatttgcttttgacaacagaggctatgatggtagcctcaatgacctcaccaaaccatgccttgaagttagttcggttgtggtaggaattataggtatgacctgccagtcatgcgtccagtccattgaaggaaggatctcaaagataccaagtgtggtcaacataaaggtttatcttgataaagccaatgcagaagtatgttacataccttctgaaataacggcacagaagatttgcgaggaaatcgaagatatggggtttgatacccacatagatatatgcaaagaactgaaaggtggtctttataaagaggatgtagtaaaaatcagggtagagggtatgacgtgccaatcatgcgtcaatagcattgaagggaaaattggaaaaattcaaggagtagaaaaaataaaggtttctcttacaaaccaagaagcaatgattgtttattgcccgcagtacatccagccagaagacctacaaaaacggatcgaagatatgggctttgttgcttctgtcaaatgcaaacctgatccaacaaaactgggaccaattaatattgaacttctacaaagtatctctacagaccccaagcttaggaatacagttggacctataggtaacacaatagcaagaactattcatatagaagggatgcattgtaaatcatgtgtacacaacattgagggctccatttctgagctccctggtgttcagaacattaaagtctctctggagaacaaaaatgctgtaatatctcttgtccaggatcttactagtctactgatgttaaaagaagtaattgaagctcttccaccaggacattttaaggtgacccttccagaagaagatgaaccgagtcctgcattacgaactaaatcaacccttcattcatctcctaatcaaatcgaaaggaccaccacaaggacagcgtccatccagatcggtggaatgacttgtgggtcttgcgtgtcaacgattgaagatacgatctctcaaagaaagggtgtccaatccatatctgtgtcattacctgaggggatgggaacaattctctatattcatacagaaactaactctggagaattaagactggctatagaagacatgggctttgacgctactcttatttctgattcactattggctaccagcggtgaccagttaagctttccagtgaagcaaaagttacaagagacaaccaagaatgcaaatctccaccaagattacattgtggatgtcctccctaaaaaacctcacctagacatccctcttatggaaaaccgtacgccccagaaatgtgtcttacaagtcacagggatgacgtgtgcctcatgtgtctccaacatcgagaggaacctgaagaagaaagatggtattatttctgtgctggtggcactgatggctggtaaagcagaggtcaaatacattgcagaccagattgaaccatctgaaatcgcacagcttattgaaaacctgggctttggagctgttgttttggaagattgcactgcatcccatggtagtatcgagctggtgattacaggtatgacatgtgcctcatgtgttcacaatattgaatcacgactgataagaatgccaggtattctacaagcctcagtggctcttgccacctgcaaagctcaattcaagtttgatcctgaaattgttggtcctcgagacatcatacaagtcattgaggggattggatttcaagcctctttatctagaagagagccatcagttcacaacctggaccataaagaagaaataagacagtggagaaagtcctttttgttcagcctcatatttggcatccccgtcatctgcatcatggtctacatgttaatcgccaacagtaatcaccatggacaatcagcgctggacaacaacatcgtccctggtttatccataatgaacctcattttctttatactttgcacattagtccagacctttggcggatggtacttctatgtccaggcttacaagtctctgaaacacaaggcagccaatatggatgtactcattgtcctggctaccaccattgcctatatttactctgtggttatcctgattgtggccattgtggagcaatcagcccagagccctgagaccttcttcgatacaccccccatgttgttcatgttcattgctctggggagatggcttgaacatatagcaaagagtaaaacttcggaagctcttgccaagctaatatccctgcaagctacagaggcgaccgttgtgacactaggacccaatcactcagtactaagggaagaacaagtagctgtggatcttgttcagaggggagatattgtgaaagttgttcccgggggaaaatttcctgtggatgggaaagttattgaagggacatctatggcggacgagtccttaatcacaggtgaacctatgcccgtgaggaagaagccagggagcatagtcatcgctgggtctattaacgcccatggatcagtccttgtagaagcgacacacgtgggctctgacaccaccctggcgcaaatagtcaagctggtagaagaggctcaaatgtccaaggcaccaatccaacagctggcagataagataagtggctacttcgtccccttcataattagcatttccgttgtgacattgattggatggactaccgtcggctttgtgaattttgatgtcatactgaaatatttcccagattacagtaaaaccatctcgaaggctgagataatcatacgggttggcttccagactgccatcactgtgctgtctattgcctgtccctgtgctttaggtctggctactcccacggcagtgatggtgggtactggagtagctgcacaaaatggcatccttatcaaaggtggagagccgctggagatggcacataagataaatacagtgatgtttgataagacagggacaataacacatggggtccctaaggtaatgagagtgctgctgctgggtgatgtagtcaggatgcccctgagaagaatgcttgccgtggtcggtactgcagaagccagcagtgagcatcccctgggaatggccatgacgaagtactgcaaggaggagcttgggacagggtcactgggatattgtacagatttccaggcggtcccgggatgcggaatccgctgtaaagtgaataatgtggaaagtgtcctggtgcagagtgagaagggcctaaatgagcagaatgcatacataagtggcctcacgaggccacacgaggatggatccctaatcattgctcccgagctacaaggtgcctcagctcctcaaccgcattctgtgttaattggaaaccgggaatggatgagacgcaatggactgcacatttccagtgatgtagatgaagctatgactagtcatgaaatgaaaggacagactgcagtgctgatagctatagatggtgcattgtgtggaatgatcgctattgcggatacagtaaagcaggaggcggctcttgctgtgcacacactgaaagcaatgggagtggatgtagtactgatcaccggagacaaccggaagaccgccaaggcaatcgccactcaggtcgggatcaacaaggtgtttgcagaagttcttccatctcacaaggtggcaaaagtggaagcattacaaaacgaagggaagaaagtagccatggtgggcgatggcgtcaatgactctcctgcattggccagggctgatattgggattgccattggcacaggaacagatgttgccattgaagctgcagatgttgttcttatcagaaatgatttgcttgatgttgtcgccagcattcacctctcaaagaggacggtgagaagaataagaattaacttcatgtttgcgctcatctataatcttgtcgggattcccattgcagcaggtgtgttcctgccggtgggattggtgttacagccatggatgggatcagcggctatggctgcctcctctgtctcagtggttttgtcctctttgcaactgaagtgctacaggaaaccagaatctgagagatatgaagctcgagcccaggggcgcatgaagcctcttaccccatcacagataagtgttcatgtggggatggatgaccggagaagagatatcccaatggccagcgcctgggaccagatcagctacatcagccaagcgtcatccagcagaaacacgcggcaagattcactaacaggcgacagacaagacaagtggtctcttctcctcaacgaggctgaggatgatctgtacatgtagtgtcatgggtttacaccaagacatgaaggaccattatctataataatgagactactcatcacatgcactggaatagtgagcaaaactgctacactgaagcactataggaagagcaaaatggatagaagtgtatagatgagaacatgctggtgttcttggtttgtattttcaagttctttaatggatataattgcaaagtgctcgcaaaaaaaggaactttgttcttggcagaatataagaagttaattacagagggattttatttctatagtttactgcactgtgggtaggttactagccacctctgtagtctgtcagctgtggttggtctccttcctaaaagagcttgtaagcgctgctcagattcttacagaatctcgctgcatccagtctgaattattgtgcatgcacgcctgcgatggtgcggacacagaacggttcctgtttgcagcacataccaatagggttttacctaacaacaattatattggtgcggactgcttgctcacctgatagggttgtgtgttcacaacatttttaaggcataaataatccacttgctgcaagaccacgggtcacaaagctaggaaaggtgctgctgtgaaaaaatgaagtcatttttgttctctcctggtggagatccacggaaagcatccagaacacagcatacaagaaatgtttaatatctatgcttttcgaaattatccaacttgaataacttggaaacgtgtagatattagaccgtgtttcttctatcctgtgtgtcctggatcctttctgtggatctccacctgcagcgcagaaaaaaaaaccttaattttgtagcatgtttccagcccggtgcagtgcacatttcgtgccagtggtggcgctatgctgctggttagaacaagctatttagcaggagtgcatcgcctcccaacaagccagaagcagcgaggccgaggagcgatgccctcctgaaaggcttaaatcttctcttcgtattacacatgatttcgctaatgactatatctggtttctaattattagcatagtctgattcacccatccgaggtccaagtacagactgtgattcacaagctggctgctggggtcctcacccaaattcaacaccccccatatatgcccatgaggcttttgaaagtctgatcaggagaccagcagctggtcgcttcactgcactcgaaaaacggatgtgtgaatctgaccttagacacaagtttatatgctgtaatacccaatattctgtctctataatatataagattacaaaaacatggctgattttttaaaagagaaatatggattgtgtgtgattctgtgcctcacttccactgacgagaccgggttggagctgcagtatcctacacaacccttagacgagcgcggctctaggcttccaattctggatatccccccttttaagaagaataacagccaggtgagagagtgcagtgtgctatttaatctgtggattatagttgtatcaggaggcccacgtgatcaggaatctatggtataacctatcaatatgcgtgaaggggaaacgcgtaggtctgcgcatctcaacactcgttccggctgctgtactgatgaaggcattctaggaaacgcttagtaatgtaaatcactaatttgtgctgcaaagagttaaactaccaagtgcttgaagggttaaattatatatatatatatatatatatatatatatatatatatatatat

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