LOCUS       XM_075592003            7183 bp    mRNA    linear   VRT 22-JUL-2025
DEFINITION  PREDICTED: Ascaphus truei ATPase copper transporting beta (ATP7B),
            transcript variant X6, mRNA.
ACCESSION   XM_075592003
VERSION     XM_075592003.1
DBLINK      BioProject: PRJNA1291731
KEYWORDS    RefSeq.
SOURCE      Ascaphus truei (coastal tailed frog)
  ORGANISM  Ascaphus truei
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Amphibia; Batrachia; Anura; Ascaphidae; Ascaphus.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_134485) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_040206685.1-RS_2025_07
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.4
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 07/16/2025
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7183
                     /organism="Ascaphus truei"
                     /mol_type="mRNA"
                     /isolate="aAscTru1"
                     /db_xref="taxon:8439"
                     /chromosome="3"
                     /sex="male"
                     /tissue_type="kidney, liver"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Polk County, Oregon"
                     /lat_lon="44.796144 N 123.527922 W"
                     /collection_date="2020-07-15"
                     /collected_by="Mark Leppin, R. Bruce Bury"
     gene            1..7183
                     /gene="ATP7B"
                     /note="ATPase copper transporting beta; Derived by
                     automated computational analysis using gene prediction
                     method: Gnomon. Supporting evidence includes similarity
                     to: 24 Proteins, and 100% coverage of the annotated
                     genomic feature by RNAseq alignments, including 3 samples
                     with support for all annotated introns"
                     /db_xref="GeneID:142490142"
     CDS             69..4451
                     /gene="ATP7B"
                     /codon_start=1
                     /product="copper-transporting ATPase 2 isoform X3"
                     /protein_id="XP_075448118.1"
                     /db_xref="GeneID:142490142"
                     /translation="

MPVSLSSKRHGASSTYKTLSSTGSPECARKPALKPHFAFDNECFDGGLDDFPTDMSSVVVSIVGMTCQSCVQSIEGRISKVAGIVSIKVCLEQNNAVLKYIRSAITPQEICEEIEDMGFDASMSAIKETPPPLKPGYSGDDFIKIRIDGMTCQSCVNTIEGKIGKLQGVQKIKVSLSSQEAVIVYQPYVIQPEDLKKYIDSMGFEASIKSRPDPAKIGTIDIERLQNLSTQHNVDALNNSPIFTMDNTETVTLGIEGVHCKSCVHNIERCISGLAGVQSIKVSLENKNAVVCFNPGITTTFFSLKEAIEALPPGEFKVALPLGVKESPESKTKSNWSSSRKQDQLLGTTTALMSIGGMTCGSCVSSIENLLLQRKGVLMASVCLADGIGTIHYNPSETKSEELRAAIEDMGFDATLISDTLPSVNCSQTDSTAPENKQSTLQNTKKIDPCGDYILDVLPKKSHTDFSAVDIAPQKCFLQVTGMTCASCVSNIERNLHRKDGIVSVLVALMAGKAEVKYKPDRIQPFEIAQLIENLGFGAAVMEDYTGTDGSIELFITGMTCASCVHNIESKLMKSRGILYASVALATCKAQVKFDPETVGPRDIIGIVTGLGFQASLAKRDITAHNLDHKEEIKQWRKSFLFSLIFGIPVICLMVYMLFTDNKKHNPMVLERNIIPGLSIMNLIFFILCTFVQSLGGRHFYVQAYKSLKHRATNMDVLIVLATTIAYVYSVVILIVAIVEKAEQSPVTFFDTPPMLFMFIALGRWLEHIAKSKTSEALAKLISLQATEATVVTHGPNHSIVREEQVAVELVQRGDIVKVVPGGKFPVDGKVIEGSSMADESLITGEPMPVRKKPGSTVIAGSINAYGSVLVEATHVGSDTTLAQIVKLVEEAQMSKAPIQQLADKISGYFVPFIIIISIVTSLTWITIGFVNFDIIVKYFPSYSKNISEAEVVIRVAFQTSITVLSIACPCALGLATPTAVMVGTGVAAQNGILIKGGEPLEMAHKIKTVMFDKTGTITHGVPKVMRVLLLGDVVKLPLKMMLAVIGTAEASSEHPLGMAVTKYCKEELGTDSLAYCTDFQAVPGCGISCKVSNIESVLVQNEEGLNEQNSYLSGLINTQDHPVIIMPESEGAAAPQTHSALIGNREWMRRNGLHISNDVDEAMTSHEMKGQTAILVAIDGELCGMIAIADTVKPEAALAVHTLKAMGVDVVLITGDNRKTAKAIATQVGINKVFAEVLPSHKVAKVQALQSEGKTVAMVGDGVNDSPALAKADIGIAIGTGTDVAIEAADIVLIRNDLLDVVASIHLSKRTVRRIRINFIFALIYNLLGVPIAAGLFMPLGLVLQPWMGSAAMAASSVSVVLSSLQLKCYKKPDSKMYEARAQGRMKPLTPSQISVHIGMDDRRRDLPKTNAWDQISYISQVSLSSLSSAQQTRQNTLAGESPDKWSLLINERDEDQYM"

     misc_feature    243..434
                     /gene="ATP7B"
                     /note="Heavy-metal-associated domain (HMA) is a conserved
                     domain of approximately 30 amino acid residues found in a
                     number of proteins that transport or detoxify heavy
                     metals, for example, the CPx-type heavy metal ATPases and
                     copper chaperones. HMA domain...; Region: HMA; cd00371"
                     /db_xref="CDD:238219"
     misc_feature    order(261..269,276..278)
                     /gene="ATP7B"
                     /note="metal-binding site [ion binding]"
                     /db_xref="CDD:238219"
     misc_feature    498..689
                     /gene="ATP7B"
                     /note="Heavy-metal-associated domain (HMA) is a conserved
                     domain of approximately 30 amino acid residues found in a
                     number of proteins that transport or detoxify heavy
                     metals, for example, the CPx-type heavy metal ATPases and
                     copper chaperones. HMA domain...; Region: HMA; cd00371"
                     /db_xref="CDD:238219"
     misc_feature    order(516..524,531..533)
                     /gene="ATP7B"
                     /note="metal-binding site [ion binding]"
                     /db_xref="CDD:238219"
     misc_feature    822..1001
                     /gene="ATP7B"
                     /note="Heavy-metal-associated domain (HMA) is a conserved
                     domain of approximately 30 amino acid residues found in a
                     number of proteins that transport or detoxify heavy
                     metals, for example, the CPx-type heavy metal ATPases and
                     copper chaperones. HMA domain...; Region: HMA; cd00371"
                     /db_xref="CDD:238219"
     misc_feature    order(840..848,855..857)
                     /gene="ATP7B"
                     /note="metal-binding site [ion binding]"
                     /db_xref="CDD:238219"
     misc_feature    1110..1322
                     /gene="ATP7B"
                     /note="Copper chaperone CopZ [Inorganic ion transport and
                     metabolism]; Region: CopZ; COG2608"
                     /db_xref="CDD:442020"
     misc_feature    1500..1688
                     /gene="ATP7B"
                     /note="Heavy-metal-associated domain (HMA) is a conserved
                     domain of approximately 30 amino acid residues found in a
                     number of proteins that transport or detoxify heavy
                     metals, for example, the CPx-type heavy metal ATPases and
                     copper chaperones. HMA domain...; Region: HMA; cd00371"
                     /db_xref="CDD:238219"
     misc_feature    order(1515..1523,1530..1532)
                     /gene="ATP7B"
                     /note="metal-binding site [ion binding]"
                     /db_xref="CDD:238219"
     misc_feature    1725..1916
                     /gene="ATP7B"
                     /note="Heavy-metal-associated domain (HMA) is a conserved
                     domain of approximately 30 amino acid residues found in a
                     number of proteins that transport or detoxify heavy
                     metals, for example, the CPx-type heavy metal ATPases and
                     copper chaperones. HMA domain...; Region: HMA; cd00371"
                     /db_xref="CDD:238219"
     misc_feature    order(1743..1751,1758..1760)
                     /gene="ATP7B"
                     /note="metal-binding site [ion binding]"
                     /db_xref="CDD:238219"
     misc_feature    1980..4115
                     /gene="ATP7B"
                     /note="P-type heavy metal-transporting ATPase, similar to
                     human copper-transporting ATPases, ATP7A and ATP7B;
                     Region: P-type_ATPase_Cu-like; cd02094"
                     /db_xref="CDD:319783"
     misc_feature    order(2973..2975,2979..2981,4044..4046)
                     /gene="ATP7B"
                     /note="putative Cu binding site [ion binding]; other site"
                     /db_xref="CDD:319783"
     misc_feature    order(3105..3113,3315..3317,3495..3503,3591..3593,
                     3711..3719,3777..3779,3786..3788,3795..3797,3852..3854,
                     3861..3863)
                     /gene="ATP7B"
                     /note="putative ATP binding site [chemical binding]; other
                     site"
                     /db_xref="CDD:319783"
     polyA_site      7183
                     /gene="ATP7B"
                     /experiment="COORDINATES: polyA evidence [ECO:0006239]"
ORIGIN      

tttgtccattgagtttctggacagttacttgaatctacactcctcaccaaaactgggtcaggaaaaacatgcctgtctctttgtccagtaagagacatggtgcatcatcaacttacaaaactctgtcaagtactggctctcctgaatgtgcacggaaaccagcactcaagccacattttgcctttgacaacgagtgctttgatggtggtctagatgatttccctactgatatgagctctgttgtggtcagtattgttggcatgacctgccaatcgtgtgttcaatccatcgagggaagaatctccaaagtagcgggcatagtgagcatcaaggtgtgcttagaacagaacaacgctgttctaaagtacatacgttcagcaataactcctcaggaaatttgtgaggaaatagaagatatggggtttgatgccagtatgtcggcaataaaagagactcctccacctctgaaacctggatattctggagatgatttcattaagatcaggatagatggtatgacatgtcaatcttgtgtcaacacaattgaagggaaaataggcaaacttcaaggggtgcagaaaatcaaagtgtctctatcgagccaagaagctgtcattgtgtatcagccatatgtcattcaaccagaagacctcaagaaatacatagacagcatgggatttgaggcttctatcaagagcagaccagatcccgcaaagattggaacaattgacatcgaacgcttgcagaatttgtctacacaacacaatgtagatgctctaaacaatagccccattttcactatggacaacactgaaactgtgacacttgggatagaaggcgtgcattgcaagtcttgtgtacacaatattgaaagatgcatatctggccttgctggtgttcagagtatcaaagtgtcccttgagaacaaaaatgctgttgtgtgttttaacccaggtataactacaacatttttttccctaaaagaagctattgaagcccttcctccaggtgagtttaaagtagcacttccattgggtgtaaaagagagccccgaatcaaaaacaaaatctaattggtcttcatcacgtaaacaagaccagctgttgggcacaactacagcattgatgagtattggaggaatgacctgtggatcttgtgtatcatcaattgaaaacctgttattacaaagaaaaggtgtactaatggcatcagtttgtttagctgatggaattgggacaatccactacaatccatctgaaacgaagtccgaggagttacgtgcagctatagaagacatgggatttgacgccacacttatttcagatactttgccctccgttaactgtagccagactgactccaccgcacctgaaaataagcagagcaccctgcagaatacaaagaaaattgatccttgtggagattacattttggatgtccttcccaagaaatctcatacagacttttccgcagttgacatcgcaccgcaaaagtgctttttacaggtgacaggcatgacatgcgcttcttgtgtgtcaaacatcgagaggaatctgcataggaaagacggtatcgtttcagtgcttgttgcactgatggctggtaaagcagaggtcaagtacaaacccgacagaatccaaccatttgaaatagcacaactcattgaaaacttgggttttggtgcagcggttatggaagattacaccggtacggatggcagcatcgagctttttattacaggaatgacatgcgcttcatgtgttcacaacattgaatccaaattgatgaaaagccgtggtattttatatgcatccgtagctcttgctacttgcaaagctcaagtcaagtttgacccagagactgtcggtccacgagatatcatagggattgtaacgggccttggtttccaagcttcattggctaaaagggatataacggctcataacctggatcataaagaggaaataaaacagtggaggaagtcctttctttttagcctcatttttgggattcctgtcatatgcctcatggtttatatgttatttactgacaacaaaaaacacaaccctatggtgctggaaagaaacattatcccagggttatcaataatgaatctcatcttcttcattctctgtacctttgttcagtcccttggagggcggcatttctacgtccaggcttacaagtccctgaagcacagagccactaacatggacgtgctgatcgttctagccactaccattgcctatgtttactcagtggttatcctgattgtggccatcgtggaaaaggctgaacagagcccagtgacgttttttgacacccctcccatgctgttcatgttcattgccctggggagatggcttgagcatatagcaaagagtaaaacttctgaagcccttgccaagctcatatccctacaagcgacggaagccacagttgttacccatggaccaaaccactccatcgtgagggaagagcaggtggctgtagaattggtccaacgaggagatattgtaaaagttgtccccggggggaagtttccagttgatggaaaagtaattgaaggcagttctatggcagacgagtctcttatcacaggtgaacctatgcctgtccgaaagaaacctgggagcacggtcattgctggttccataaatgcatacggctcagtacttgttgaagcaacacatgttggttctgataccactcttgcacagattgtcaaattagtagaagaagctcagatgtcaaaggcaccaatccaacagctggcagacaaaataagtggatactttgttccttttatcatcattatttccatagtaacctcattgacctggatcaccattggctttgtgaattttgatatcattgtgaaatactttcctagttacagtaaaaatatctcggaagctgaagtagttatacgggtggcgttccagacctcaatcacagtgctctctatcgcctgcccctgtgctctcggactggcgactcccacagccgtgatggttggcacaggtgttgctgcacaaaatggtatccttatcaaaggtggtgaacctctggagatggcacacaagatcaagacggtgatgtttgacaagactggcacaataacgcatggagtccctaaagtcatgcgagtgctgctgctgggcgacgtggtcaagctgcctctgaaaatgatgctagcagtgattgggacggcagaggccagcagcgaacatcccttaggaatggctgtgacgaaatactgcaaagaggagcttggtacagattctttggcatattgtacagatttccaggctgtcccgggatgtggaatcagctgtaaagtgagcaacattgaaagtgttcttgtgcagaatgaggaaggtttgaatgagcagaattcttacctgagtggtctcatcaacacacaagaccaccccgtgattatcatgcctgaatcagaaggtgcagcagccccgcaaactcactctgctttaatcggaaaccgcgaatggatgcgacgtaacggtttacatatctctaatgatgttgatgaggccatgaccagtcacgagatgaaaggacaaacggcaatactggtagcaatagatggtgagctgtgtggaatgattgccatagcagacacggtgaagccggaggccgcccttgctgtgcatactctaaaggcgatgggagtagacgtggtgttaattactggggacaaccggaaaacggcaaaagcaattgctactcaggttggcatcaacaaggtttttgcagaagtccttccctctcataaagttgcaaaggtccaggcgctccagagcgaagggaagacggtggctatggtaggggatggggtcaacgattcccccgccctggctaaagctgatattggtattgccattggaactggaacagatgtggccatcgaagctgccgatatcgtcctgatcagaaatgatttattagatgttgttgccagtatccacctctcaaagaggacagtgagaagaatacggataaattttatatttgcattaatatataacctgcttggtgtacccatagctgcaggtttgttcatgccattaggattggtgttacagccatggatgggctcagctgcaatggcagcctcctcggtctctgtggttctgtcctcactgcaactgaaatgctacaaaaagccagattctaaaatgtatgaagcccgagcccagggtcgcatgaagccgcttaccccgtcgcagataagtgttcacattgggatggatgaccggagacgagacctacccaagacaaacgcctgggatcagatcagctacatcagtcaggtgtccctgtcctctctgtcttccgcccaacagactcggcagaacaccttagcaggagaaagtccagataagtggtccctgctaatcaatgaacgggatgaggaccagtacatgtgaatcactacattaagaggaagtctgataacatcatgtaaacagctctaagtgaccggcacaaacccagaaactgtgttataaagcacagtatgtttggacctgtatatccgtagcttaatatattcacaactggttaaagtttagtgctgttcttttcatcttacactgggagtagaatatccaagtatcaaatatggaataaatcctgcattgctaaaatacctttggtgcacagtgctcaagaggatgttccccatgtaaaacaatagtggcttcacataatcttacgcattggcactttgtcaatactaaagccatatattcaatacacatacagtgtacgtgaagtatatcacattcaaacaattgagttaaaatactgcgtgcaatgcatccgagcctcaggggagcttagtctagtttttggtaccacagaaataggaaaatatatagtcaggtgggggccttgaggctatttattaaaatctacagcaaatcaaaatatcacttatgagcacattcatatgtctcagagagatccacaaattccctttccccataatcactcagcatacaatacttacactgcagccagggattctgggtaatgacatacaaacgagcactcacagggtgtcactttttgcttctttaaagtcgatccctataggcccgtcgttgtactcagcttttacagcaaagggctgggttaaagatgtgcatggtcagcaaccctccagtactcggacaggcgttttgaccttttgagtctcatcagtggttactgaccttgcaatgtgaggctggtagtggccacaaaccaggcacacaacgtacattcccgtgacttacaaagataaaattggaccaactgcaaaacaatggcacaaagtatcgctggattcatgaaagttaaaaacattgctttgaagtcgatttttgttttccttgataaacatgttgctatttgtgcctatttgtgttttacaggctcttcaatgcttaattccaccaatcaacgagtttattggccagtaatgtaaatacttggtcagtaggaccaatactttttcctgtctacttctccttctgtcttaacacggcatcggacacaaaagagcattggaaacttctggatttatagcagagtggctttgtgcaatgctctacaaatataccaaacggacatgtccatctttggttttgcattgtgcaagattgtacaagattgtaccaaagtttgcaaccagggtgcataggggtgaggggagtcatttctggatccgggctaacattcagctaaaagcatgggagcctgggcactgcatctccagctgaatggcagagccatcttaagatgggtgaaaagtttgtgaacttgggaaattaggccgcacttatagtgccggcgacgtgaccgatgacgtcacccgtcgccactagtgaaagttataattcagttttaagcaacgtcgctggcgacaaggccagtgacatcacgaagggggcgggcagcgctctctgatttggtttagagacagtcacatgtggcgactgtctctaaaaaataaagttgacccggcttcaaaatttttggtcgctctgtcgctccgttgccgtcgcgcttactataagggcttgcgatggagtcaatgtatttgttttggagcgccgtcgccaggcactgtaagcgcagcctaacagacatttatggctggtgacaaattggtagttgcaagtatcttaatacaaaagcacatggaccccacctttcctgtacattttgtgaataacggtggagaatattggcaaaatgtcaccaatattcactcaaaaaataattagagaggagcatggatttgtgactgtgaattgagaaatagcctttgctcaaatgttttgctaattcacaacttgctttaaaaaattgcaggtagattttgctaagtgaattggtgtacaaatctgtacttggtatgatactgatccaagtatcggcaagtttagcactaaatctctggtcactgcggtcatacacacgagatcaggactttgcagttaaagtagctaaacactacagacgtgcacattttgggggcggaagttcgcaaaccaggtgaaatgcgccaggttttttttctttgtgaaacattttatcaagcaaatttgaagacatttgcgcaacttttttcatatattcacaaaatcctgcaaaagttgtcacatttttcagtaatctggctaaaatgttgctttcctgaaaaatacaaacattcgtggaaattttgtggaaatgtaaaacaacggaaaaaacttaagacatttgcacaaccctgctaaacacagcagaatatcaaaaactagctgagtaaattatgcttcataatactgtacatctaaaaacaaaacgttgggttactaatgaatgaatacatctgtgtatccatatctaagcaatcaattattcttgtaatgaaagatgtatattgttttttttttttatttgctttgttttcttcaattaatttttaacattgtattataatgtaagtttaagcaagtgccatacatagaattttatataaaaataatatacaactggtcaaatgttatttattctatatgtagtgtgatcattttttctgtgctactccatatgtttaatcctgcatattattctgttcatggtttcattttttatagtcccctaataaacgttgcattcctttgta

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