LOCUS       XM_072151132           13593 bp    mRNA    linear   VRT 14-MAR-2025
DEFINITION  PREDICTED: Engystomops pustulosus argonaute RISC catalytic
            component 2 (AGO2), transcript variant X4, mRNA.
ACCESSION   XM_072151132
VERSION     XM_072151132.1
DBLINK      BioProject: PRJNA1226277
KEYWORDS    RefSeq.
SOURCE      Engystomops pustulosus (Tungara frog)
  ORGANISM  Engystomops pustulosus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Amphibia; Batrachia; Anura; Neobatrachia; Hyloidea;
            Leptodactylidae; Leiuperinae; Engystomops.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_092415) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_040894005.1-RS_2025_03
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.3
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 03/12/2025
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13593
                     /organism="Engystomops pustulosus"
                     /mol_type="mRNA"
                     /db_xref="taxon:76066"
                     /chromosome="5"
     gene            1..13593
                     /gene="AGO2"
                     /note="argonaute RISC catalytic component 2; Derived by
                     automated computational analysis using gene prediction
                     method: Gnomon. Supporting evidence includes similarity
                     to: 39 Proteins"
                     /db_xref="GeneID:140132393"
     CDS             316..2901
                     /gene="AGO2"
                     /codon_start=1
                     /product="protein argonaute-2 isoform X4"
                     /protein_id="XP_072007233.1"
                     /db_xref="GeneID:140132393"
                     /translation="

MYAGAGPVIAPPPPAPPLPMPAYTFKPPPRPDFGTSGRTIKLQANFFEMDIPKIEIYHYEIDIKPEKCPRRVNREIVEHMVLHFKGQIFGDRKPVFDGRKNLYTAMPLPIARDKVELEVTLPGEGKDRIFKVAIKWMACVSLQALHDALTGRLPSVPFETIQALDVVMRHLPSMRYTPVGRSFFTASEGCANPLGGGREVWFGFHQSVRPSLWKMMLNIDVSATAFYKAQPVIEFMCEVLDFKSIEEQQKPLTDSQRVKFTKEIKGLKVEITHCGQMKRKYRVCNVTRRPASHQTFPLQQESGQTVECTVAQYFKDRHKLVLRYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIRATARSAPDRQEEISKLMRSASFNTDPFVREFGIMVKDEMTDVTGRVLQPPSILYGGRNKAIATPVQGVWDMRNKQFHTGIEIKVWAIACFAPQRQCTELHLKTFTEQLRKISRDAGMPIQGQPCFCKYAQGADSVEPMFRHLKNTYTGLQLIVVILPGKTPVYAEVKRVGDTVLGMATQCVQMKNVQRTTPQTLSNLCLKINVKLGGVNNILLPQGRPPVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDAHPNRYCATVRVQQHRQEIIQDLAAMVRELLIQFYKSTRFKPTRIIFYRDGVSEGQFQQVLHHELLAIREACIKLEKDYQPGITFIVVQKRHHTRLFCTDRNERVGKSGNIPAGTTVDTKITHPSEFDFYLCSHAGIQGTSRPSHYHVLWDDNRFSSDELQILTYQLCHTYVRCTRSVSIPAPAYYAHLVAFRARYHLVDKEHDSAEGSHTSGQSNGRDHQALAKAVQVHQDTLRTMYFA"

     misc_feature    595..819
                     /gene="AGO2"
                     /note="N-terminal domain of argonaute; Region: ArgoN;
                     pfam16486"
                     /db_xref="CDD:465134"
     misc_feature    847..999
                     /gene="AGO2"
                     /note="Argonaute linker 1 domain; Region: ArgoL1;
                     pfam08699"
                     /db_xref="CDD:462567"
     misc_feature    1000..1362
                     /gene="AGO2"
                     /note="PAZ domain, argonaute_like subfamily. Argonaute is
                     part of the RNA-induced silencing complex (RISC), and is
                     an endonuclease that plays a key role in the RNA
                     interference pathway. The PAZ domain has been named after
                     the proteins Piwi,Argonaut, and Zwille; Region:
                     PAZ_argonaute_like; cd02846"
                     /db_xref="CDD:239212"
     misc_feature    order(1156..1158,1201..1203,1243..1245,1255..1257,
                     1309..1311,1330..1332,1336..1338)
                     /gene="AGO2"
                     /note="nucleic acid-binding interface [nucleotide
                     binding]; other site"
                     /db_xref="CDD:239212"
     misc_feature    1495..2772
                     /gene="AGO2"
                     /note="PIWI domain, Argonaute-like subfamily. Argonaute is
                     the central component of the RNA-induced silencing complex
                     (RISC) and related complexes. The PIWI domain is the
                     C-terminal portion of Argonaute and consists of two
                     subdomains, one of which provides the...; Region:
                     Piwi_ago-like; cd04657"
                     /db_xref="CDD:240015"
     misc_feature    order(1906..1908,1918..1920,1954..1965,1972..1974,
                     1996..1998,2005..2007,2017..2019,2029..2031)
                     /gene="AGO2"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240015"
     misc_feature    order(2110..2112,2116..2118,2326..2328,2740..2742)
                     /gene="AGO2"
                     /note="active site"
                     /db_xref="CDD:240015"
     polyA_site      13593
                     /gene="AGO2"
                     /experiment="COORDINATES: polyA evidence [ECO:0006239]"
ORIGIN      

cccaccccttagttttacccgccctttttttcccctcaggtgacatcagtgagtccggctagcgactccgcccccaaaggaagacgcagcgaatggggttcccgaaacgccccgtgacgtcgccgccccgcccaccgcctttctcccctcagttcatgtcgcgtacagtctagagacggcggcggcggagtgcacggagctcgatggagacctgactgaggagagtttacccgccgggagccgccattagcgattcgtgggcaactttatctctattattttctcctatctgtgaaactgactggtggaatcaagatgtacgccggagccggccctgtgattgcccctccaccccctgcaccacctctcccaatgccagcatacactttcaaaccaccgccaaggccagattttggcacctcaggaaggactatcaagctgcaggccaacttttttgaaatggacattcccaaaatagagatttaccattatgaaatagatatcaagccagaaaaatgccctagaagagtgaacagggaaattgtagaacacatggtattgcattttaagggtcaaatcttcggagatcgaaagccagtgtttgacggtagaaaaaatctttacactgcgatgcctcttcccattgccagagacaaagtggaattggaagttacactgccgggggaaggaaaggatcgcatctttaaagtagcaatcaaatggatggcctgtgtgagcttacaagcgttacacgatgcattgaccggacgtcttcccagcgttccttttgagactatccaggcattagatgtggtgatgagacatttgccctctatgaggtacacgcctgtgggccgctctttctttactgcatccgaaggatgtgcaaatcctcttggtggaggtagagaagtttggtttggcttccatcaatctgttagaccatccctctggaaaatgatgctcaatatagatgtgtctgctacagcgttttacaaagcacagccagtaatagagttcatgtgtgaagttttggactttaagagcatagaggagcaacagaagcctctgactgattcccaaagggttaagtttaccaaagaaatcaaaggcttaaaagttgaaattactcactgtggacaaatgaaaaggaaatacagagtgtgcaatgtgacaagacgaccagcaagtcatcaaacattccctctccaacaggagagtgggcagacggtggagtgcaccgtcgcacagtacttcaaggatagacacaaattagtattgcgctacccccatcttccatgtttacaggtcgggcaggagcagaaacacacgtacctccctctcgaggtatgcaacatagtggcgggacaaaggtgcattaaaaaactgacagacaatcaaacctccactatgattcgtgcgacagcccgatcagctccagatcgccaagaagagatcagtaaattgatgcgcagtgcaagcttcaatactgatccctttgtccgtgagtttgggattatggtgaaagatgaaatgaccgatgtcactggcagagtccttcaacctccttcaattctttacggaggcaggaataaggccattgctacccctgtgcaaggtgtgtgggatatgaggaacaagcaatttcacaccggtattgaaataaaagtgtgggccattgcttgctttgctcctcagcggcagtgtacagaacttcacctcaagactttcactgaacagttgcggaagatatctcgtgatgccggtatgcccattcaggggcagccatgtttctgcaaatatgcccaaggtgctgacagcgtggagccaatgttcagacatcttaagaacacgtacactggtctacaactgatcgttgtaatcctccctgggaagacaccggtctatgctgaggtgaagcgcgttggagacactgtgttggggatggcaacgcagtgtgtgcagatgaagaatgtgcagcgaaccacgccacagaccctgtcaaacctctgcttaaagattaacgtcaaattaggaggagtcaataatatcctcttaccccagggaagacctccagtgtttcagcagccggtcatatttttaggagcagatgtcacacatcctcccgcaggggatggaaaaaagccttcaatcgcagcggttgtgggtagcatggatgctcatcccaatagatactgtgccactgtgagagtgcagcagcaccgacaagagattatccaggacctggccgccatggtccgagagctgctcatccagttctacaagtctacacgattcaagcccacaaggatcattttctatagggatggcgtctctgaggggcagtttcagcaggttttacatcacgagctgcttgccatcagagaagcttgcattaaattggagaaggattatcagcctggaatcaccttcatcgtagtacagaaaaggcatcatacgagactcttctgtacagataggaacgaacgggttggaaaaagtggaaatattcctgctggtaccacagtggacacaaaaatcacgcacccgtcagagtttgatttttatctatgtagtcatgctggtatacagggaacaagccgaccctcgcactatcacgttctctgggacgataaccgtttctcctcggacgaactgcaaattctaacttaccagctgtgccacacttatgttcgctgcactcgatccgtctcaatccccgctcctgcatactacgcacacttagtcgcttttagagccagatatcacctggtggataaagaacatgacagcgctgaaggcagccacacttctggtcagagtaacggccgagaccatcaagcacttgctaaggcggttcaagttcaccaggacacgctgcgtacaatgtactttgcttaactggtgctggaagcaggcactacacgaaggagcattcacgccagaccagctgcacttacctgtaacgtcgtctcagctacagggacatccagtaaaagtgcaatcaactcatagccctgtcagtctcctacggccctcggcgtcccaccagccttccgtccagaccctgaattttacttttatcttgaacttcatacctgcattgaatcatgtgaggtttgtgaaaaaccatataaatatatatatagaagcgtttacaaaaaaaaaattacccttttcaacacaaaggttaaactttactgttctctcagggctcacaaaaacaaatgaaaaacgctatgaaataattttttctctgtggaaattttgtgtcgaatatcagataaatgttacagcctagggataaaaaataacacacaaaaaagaggaagaaaatattgacatttttaaagccttaaaatattttaagtgaatttatcaagattttattacaagaaaaaaaaatcttttttttgttttttttttgttttcttcctctgacatttgtgtcaaatattctagtacataaagttatgtatcggaataaagtcggtcactaattgctatggaggtcaggcctgacctcttggtgaatataatagtattcattagggttttttttgtttttaataattgtgccattcagaaaaaaaaaaccctgaggtttagtttttaattattgcgttttctttgtggctgcactcatacttttaatatatatttttccccgaggttgctgactaatacgggtctgtggcaatgtggacagtacaatcatgttaagctaatgtacagaaaaaaataatctattttagcagtattaatgtgagatgacgtgctatttattttatttacacagatcaccagatttcttttttttaacctatggttttatgtacttgggcttaaatagtgtcctgtttaacccttgcactgacaatgcgacctgtagagccttttacaggaccaccggcagtgaatggattaaaaaaaaaaattgaaaaaaatttttgaaaaataaaaagtaaaacctcattttatgtaactgcttcatccctccagaggactcgtaaatgagactttgcaatgcaataaaaagcaatgtctctttaatccctctggttgctaacagggtttgttgtgaggagcaaaatcctaagggttaaagagaaaaaaattgtaaaactctattcttttttttttaaatgcaaaaaatacaaaaaggctgcagcgtgtaacagcagctaagatctggcttgtaaccaccaatactgacttgttcctcgctcctctgtctttggggttcccgcactgcgtccctttgtaacatccatcgttataagggaatcctcatgccgtcgcctcggagacgttcatatgcaaacctgtattagttgtctttttttattttctaataaatatctatttatctttttcccactcctcctatgctttaaggaatatgcaattctgtaatgatattcaatacaaggactcagtttttaagcaatatcatgacaagatttctacagtgctgttgtgtggctctatctggaaccaggggcacacaattttattctgtattgagccttaacactctaaacattggcatagaattgtggctgcggctctgcagctcacgctaacctacagctcccagcaagcaatgggccgtgttgggagttgtagtgcggccggagagtaatgctgtgtagtgtaagttttcattggcctaattgtaaattgaaacctaggatgttgcaggggatagggatgtatttaaatttttgtagtttttttttttttattttaaattgcccattttctccttgtgggggtaaatgattcacttgttaaatgttttaggtgctatatatttactgtctgctttgcaagcgctggtctaatcaaccaatggcaaacacggtttgtcatggccttgatagttccatagatcaaacagttagaacaatgatctccacctttaaataatgaagtcttatccgctatccttatcaactgatggagattgtgcagtgctcagagggggaaggggcggg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