LOCUS       XM_072116265            3673 bp    mRNA    linear   VRT 14-MAR-2025
DEFINITION  PREDICTED: Engystomops pustulosus dicer 1, ribonuclease III
            (DICER1), transcript variant X2, mRNA.
ACCESSION   XM_072116265
VERSION     XM_072116265.1
DBLINK      BioProject: PRJNA1226277
KEYWORDS    RefSeq.
SOURCE      Engystomops pustulosus (Tungara frog)
  ORGANISM  Engystomops pustulosus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Amphibia; Batrachia; Anura; Neobatrachia; Hyloidea;
            Leptodactylidae; Leiuperinae; Engystomops.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_092417) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_040894005.1-RS_2025_03
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.3
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 03/12/2025
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3673
                     /organism="Engystomops pustulosus"
                     /mol_type="mRNA"
                     /db_xref="taxon:76066"
                     /chromosome="7"
     gene            1..3673
                     /gene="DICER1"
                     /note="dicer 1, ribonuclease III; Derived by automated
                     computational analysis using gene prediction method:
                     Gnomon. Supporting evidence includes similarity to: 3
                     Proteins"
                     /db_xref="GeneID:140069953"
     CDS             337..3582
                     /gene="DICER1"
                     /codon_start=1
                     /product="endoribonuclease Dicer isoform X2"
                     /protein_id="XP_071972366.1"
                     /db_xref="GeneID:140069953"
                     /translation="

MASLQLMTPASSPMGPFFGLPWQQEAIHDNIYTPRKYQVELLEAALDHNTIVCLNSGSGKTFIAVLLTKELSYQIRGDFNKAAKRTVFLVNSAKQVAQQVSAVRTHSDLTVGEYSSPEVVESWSKEKWQEEFVTHQILVMTCHIFLHVLKRENLSLSNINLLVFDECHLAIQDHPYREIMKICESCQTCPRILGLTASILNGKCDPRDLKEKIQKLEEILKSNAETATDLVVLDRYASQPCEIVLDCGPYHDRSGLYQRLLSELDDALHFLDDCNIPILSKERDSTSISKQILSDCQAVLLVLGPWCADKVAGMMVRELQKYIKHEQEELHRKFLLFTDTTLRKIHALCEEHFSPASLDLKFVTPKVIKLLEILRKYKPYERQQFESVEWYNNRNQDNYVSWSDSEDDDDEDEEIEEKEKPETSFPSPFTNILCGIIFVERRYTAVVLNRLIKEAGKQDPELAYISSNFITGHGIGKSQLRNKQMEVEFRKQEEVLRKFRAHETNLLIATSIVEEGVDIPKCNLVVRFDLPTEYRSYVQSKGRARAPISNYIMLADTERIKGFEEDLKTYKAIEKILRNKCSKSMDCGDTEPDSTVDDDGIFPPYVLRQDDGSPRVTLNNAIGHINRYCARLPSDPFTHLAPKCKTREVSCGVCQSTLYLPINSPLRAPIVGPSMNCARLAERAVALICCKMLHQIGELDDHLMPVGKETVKYEEELDLHDEEETSVPGRPGSTKRRQCYPKAIPECLRNSYPKPGQSCYLYVIGMVLTTPLPDELNFRRRKLYPPEDTTRCFGILTAKPIPQIPHFPVYTRSGEVTISIELKKSGFTLTLQQLELITRLHQYIFSHILRLEKPALEFKPTAADAAYCVLPLNVVNDCGTLDIDFKFVEDIEKSEARTGIPSSQYSAENPFIFKLEDYQDAVIIPRYRNFDQPHRFYVADVYTDLTPLSKFPSPEYETFAEYYKTKYNLDLTNLNQPLLDVDHTSSRLNLLTPRHLNQKGKALPLSSAEKRKAKWESLQNKQILVPELCAIHPIPASLWRKAVCLPSILYRLHCLLTAEELRAHTAIDAGVGVKSLPEDFR"

     misc_feature    430..1023
                     /gene="DICER1"
                     /note="DEXH-box helicase domain of endoribonuclease Dicer;
                     Region: DEXHc_dicer; cd18034"
                     /db_xref="CDD:350792"
     misc_feature    order(430..441,448..450,499..522,643..645,832..834,
                     925..927)
                     /gene="DICER1"
                     /note="ATP binding site [chemical binding]; other site"
                     /db_xref="CDD:350792"
     misc_feature    order(607..612,679..684,757..759,763..768,775..777,
                     850..858)
                     /gene="DICER1"
                     /note="nucleic acid binding site [nucleotide binding];
                     other site"
                     /db_xref="CDD:350792"
     misc_feature    1117..1401
                     /gene="DICER1"
                     /note="Partner-binding domain of the endoribonuclease
                     Dicer; Region: Dicer_PBD; cd15903"
                     /db_xref="CDD:277191"
     misc_feature    order(1132..1137,1144..1146,1150..1155,1330..1335,
                     1342..1347,1354..1356,1363..1368,1375..1377)
                     /gene="DICER1"
                     /note="Trbp binding interface [polypeptide binding]; other
                     site"
                     /db_xref="CDD:277191"
     misc_feature    1420..2001
                     /gene="DICER1"
                     /note="C-terminal helicase domain of the endoribonuclease
                     Dicer; Region: SF2_C_dicer; cd18802"
                     /db_xref="CDD:350189"
     misc_feature    order(1654..1662,1870..1872,1927..1929,1933..1935)
                     /gene="DICER1"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:350189"
     misc_feature    order(1882..1884,1888..1890,1963..1965,1969..1971)
                     /gene="DICER1"
                     /note="ATP binding site [chemical binding]; other site"
                     /db_xref="CDD:350189"
     misc_feature    2197..2463
                     /gene="DICER1"
                     /note="Dicer dimerization domain; Region: Dicer_dimer;
                     pfam03368"
                     /db_xref="CDD:460900"
     misc_feature    2965..3333
                     /gene="DICER1"
                     /note="PAZ domain, dicer_like subfamily. Dicer is an RNAse
                     involved in cleaving dsRNA in the RNA interference
                     pathway. It generates dsRNAs which are approximately 20 bp
                     long (siRNAs), which in turn target hydrolysis of
                     homologous RNAs. PAZ domains are named...; Region:
                     PAZ_dicer_like; cd02843"
                     /db_xref="CDD:239209"
     misc_feature    order(3154..3156,3193..3195,3211..3213,3223..3225,
                     3277..3279,3301..3303,3307..3309)
                     /gene="DICER1"
                     /note="nucleic acid-binding interface [nucleotide
                     binding]; other site"
                     /db_xref="CDD:239209"
ORIGIN      

cggtcacatgaccgtgacgtcaggcggcgagctgttggcggcggccggtggatgggcccgtcatcctcccgtagctaggatcgcacgctctgggagaggagcccggtggccggcctcgtacctggcagtgcggggtcacatgagcgcggcccgggggtaactaggtctggcaaactctgtggcggctcctaatctgctctaaggtgaattactcagttatgacgatgttacaggatggttcaagcctttttgcatttgtgagctatcagacagaaagagagagctgtgctgaaccggaactgcaatgatctgaccactggatgaaagaccgtcagcatggcaagcctgcagctcatgaccccggcctcctcacccatgggtcctttctttggactgccttggcagcaggaggcaattcatgacaatatatataccccaaggaaatatcaggtggagctgctggaagccgctttggaccacaatacaatagtgtgtctgaactctggctcagggaagacgttcattgccgtactacttactaaagagttatcctatcagatccggggagactttaataaagctgccaagagaacagtgttccttgtcaattctgctaagcaagttgcccagcaagtgtctgccgtacgcactcattcagatctgacagtcggtgaatattccagcccagaagttgtggaaagttggtcaaaggaaaaatggcaggaggagtttgtgactcatcagattctggttatgacttgtcacatattcctccatgtcctaaagagagagaacctgtctttatcgaacatcaacctcctggtgttcgacgaatgccaccttgccatccaggatcacccatatcgtgagatcatgaagatctgtgaaagttgccagacatgtccacgaatcttgggactgaccgcctccatactgaacgggaaatgtgacccccgcgaccttaaagagaagatccagaaactagaggaaattttgaagagtaatgctgagacggcaactgatctggtggttttagacaggtatgcctcgcagccttgtgagattgtgttggactgtggcccctatcatgacagaagcggactctatcagaggctcctatctgaactggacgatgcacttcacttcctggatgactgcaacattcctattctttctaaagagagagattcaacatcaatctccaaacagatcctgtcagactgccaggcagttttgctcgttctgggtccgtggtgtgctgataaagttgctggaatgatggtgagagaactgcagaaatatataaaacatgagcaagaagagctgcatcggaagttcctgctgtttacagacaccaccctgaggaagatccatgctctctgtgaggagcatttctccccagcatcccttgacttgaaatttgtcacccccaaagtcatcaagcttttggagatcctacggaagtataaaccctatgaacggcagcagtttgagagtgtggaatggtacaacaaccgcaatcaggataactatgtgtcatggagtgactctgaggacgacgacgatgaagatgaggagatagaggagaaagagaagccagagactagctttccctctccatttaccaacatcttgtgtggaatcatctttgtagagcgaagatacaccgcagttgtcttgaataggttgatcaaggaggccgggaagcaggaccctgagctggcatacattagcagcaacttcataactggacacggcattgggaagagccagctccgaaacaagcagatggaagtagagttcagaaaacaggaggaggttctccgtaagttcagagctcatgaaaccaacctgctcattgccaccagcattgtggaggagggggtggacatccctaaatgcaacttggttgtccgatttgacctgccaacagagtacaggtcgtatgttcaatccaaaggacgagcgcgggcccccatctcaaactacatcatgttggcagacacagagcggataaaggggtttgaagaggatctgaaaacctataaagccattgagaagatcctgcgcaataagtgctccaagtccatggattgtggtgacacagagccggactccactgtggatgatgacgggatctttcctccatacgtgttgcggcaagatgacggcagccccagagttaccctaaacaatgctattggtcacatcaaccgttattgtgcccggctgcccagtgacccattcactcacctcgctccaaaatgtaaaactcgggaggtgtcctgtggtgtctgccagtcaaccctttacctgccaatcaactccccgctcagagcccccattgttggcccatccatgaactgtgccaggcttgccgaacgagcagtagccctcatatgctgcaagatgctgcatcagataggtgaactggatgaccatttgatgccagttggcaaggaaactgttaagtatgaggaggagcttgaccttcacgatgaggaggaaaccagtgtcccaggccgaccaggctcaaccaagaggaggcaatgctatccaaaagctatccccgagtgtctgcggaacagttatcccaagcccggccaatcatgttacctatatgttataggaatggtgttgacaacccctctacccgatgaactgaacttcaggagacggaagctctacccacctgaggacacaactagatgcttcggcatcctgactgccaaacccatacctcagatcccacacttccctgtctacacccgttctggagaagtgaccatatcgattgagctgaagaagtctggatttactttaacattgcaacagcttgagctgatcacccggctgcaccagtacatattctctcacatccttcgtcttgagaaacctgcactagaattcaaacccacagcagccgatgctgcttattgtgttctacctcttaatgttgttaatgactgcggcactttggatatagattttaagtttgtggaagatattgagaaatcagaagctcgtactggcattcctagttcacagtattcagctgagaatccttttattttcaagctggaagactaccaggatgcagttatcattccaagatatcgcaattttgaccagcctcatcgattttatgttgcggatgtatacactgacctaactccactcagtaaattcccatccccggaatatgaaacatttgctgaatattataaaacaaagtacaacctcgacttgacaaacctcaaccagccactcttggatgttgaccacacctcatcacgactgaacctgctgaccccacggcacctgaatcagaaggggaaggctctacctttaagcagtgcagaaaaacgcaaagccaagtgggagagtttgcagaacaaacagatccttgttccagagctctgtgctattcatcccatcccagcgtccttgtggaggaaagcggtgtgcttgccaagcattctatatcggctgcattgtctcctgacagccgaggagctcagagcccacacagcaatagatgcaggcgttggagtgaaatcccttcctgaagatttccggtgagtaactgcaatctttaccgtcttggaaagaagaaaggatcaccaagccgcatggttgtgtcgatatttgatccacctgtcaactggctccc

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